Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,918,415 G→A 100% P75P (CCC→CCT)  syd ← SecY‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,918,4150GA99.2% 727.6 / ‑2.8 235P75P (CCC→CCT) sydSecY‑interacting protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (119/113);  total (120/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 243 total aligned reads displayed.

TCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGC  >  CP009273/2918270‑2918559
                                                                                                                                                 |                                                                                                                                                
  tGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGtt                                                                                                                                              <  2:2924538/148‑1 (MQ=255)
  tGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGtt                                                                                                                                              <  1:436737/148‑1 (MQ=255)
  tGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGtt                                                                                                                                              <  2:2595782/148‑1 (MQ=255)
   gAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTg                                                                                                                                             >  1:2014284/1‑148 (MQ=255)
    aGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGt                                                                                                                                            <  2:1240665/148‑1 (MQ=255)
    aGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGt                                                                                                                                            <  1:2737363/148‑1 (MQ=255)
    aGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGt                                                                                                                                            <  2:50706/148‑1 (MQ=255)
    aGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGt                                                                                                                                            <  2:1981384/148‑1 (MQ=255)
     gTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTa                                                                                                                                           <  2:860059/148‑1 (MQ=255)
      tCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTAt                                                                                                                                          <  1:227544/148‑1 (MQ=255)
         ccAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATcac                                                                                                                                       >  1:2659815/1‑148 (MQ=255)
          cAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATcaca                                                                                                                                      <  2:2729660/148‑1 (MQ=255)
           aGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACaa                                                                                                                                     <  1:1302286/148‑1 (MQ=255)
            gATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAat                                                                                                                                    <  1:3499905/148‑1 (MQ=255)
             aTGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAata                                                                                                                                   <  1:3767776/148‑1 (MQ=255)
                aCCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCa                                                                                                                                >  2:784887/1‑148 (MQ=255)
                aCCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCa                                                                                                                                >  2:1809886/1‑148 (MQ=255)
                 ccAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCaa                                                                                                                               <  1:3391324/148‑1 (MQ=255)
                  cAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCaaa                                                                                                                              <  2:3055446/148‑1 (MQ=255)
                    aTCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAg                                                                                                                            <  1:37047/148‑1 (MQ=255)
                    aTCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAg                                                                                                                            >  2:3096630/1‑148 (MQ=255)
                      cAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAgcg                                                                                                                          >  2:1186353/1‑148 (MQ=255)
                      cAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAgcg                                                                                                                          >  2:723401/1‑148 (MQ=255)
                        gATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGcgcg                                                                                                                        <  1:2371685/148‑1 (MQ=255)
                        gATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGcgcg                                                                                                                        <  1:2137149/148‑1 (MQ=255)
                         aTTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGt                                                                                                                       <  1:2421719/148‑1 (MQ=255)
                         aTTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGt                                                                                                                       <  1:3146417/148‑1 (MQ=255)
                           tttCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTc                                                                                                                     >  2:1918395/1‑148 (MQ=255)
                             tCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCaa                                                                                                                   <  2:2629148/148‑1 (MQ=255)
                                tGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACcg                                                                                                                >  1:609557/1‑148 (MQ=255)
                                 gCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGGATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACcgc                                                                                                               >  1:3545164/1‑148 (MQ=255)
                                   aCCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGt                                                                                                             >  1:3582128/1‑148 (MQ=255)
                                   aCCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGt                                                                                                             >  1:809368/1‑148 (MQ=255)
                                   aCCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGt                                                                                                             <  1:533303/148‑1 (MQ=255)
                                   aCCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGt                                                                                                             <  2:157424/148‑1 (MQ=255)
                                       gACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTAc                                                                                                         <  1:477532/148‑1 (MQ=255)
                                       gACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTAc                                                                                                         <  1:3470128/148‑1 (MQ=255)
                                         cGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAACCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTGTACAt                                                                                                       >  1:3094934/1‑148 (MQ=255)
                                          ggAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAtt                                                                                                      <  1:2847212/148‑1 (MQ=255)
                                           gAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAttt                                                                                                     <  1:1034151/148‑1 (MQ=255)
                                             aGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTg                                                                                                   >  2:3481605/1‑148 (MQ=255)
                                              gtcgtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGt                                                                                                  >  2:643552/1‑148 (MQ=255)
                                              gtcgtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGt                                                                                                  <  1:578580/148‑1 (MQ=255)
                                              gtcgtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGt                                                                                                  <  1:637524/148‑1 (MQ=255)
                                               tcgtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGtt                                                                                                 <  1:2667761/148‑1 (MQ=255)
                                                 gtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTcc                                                                                               >  1:1585956/1‑148 (MQ=255)
                                                     tCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCgg                                                                                           >  2:612991/1‑148 (MQ=255)
                                                     tCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCgg                                                                                           <  1:2240779/148‑1 (MQ=255)
                                                         tCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTaaa                                                                                       <  1:670717/148‑1 (MQ=255)
                                                          ccAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAAc                                                                                      >  2:1410442/1‑148 (MQ=255)
                                                             ggTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGc                                                                                   <  2:3194739/148‑1 (MQ=255)
                                                              gTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCt                                                                                  >  1:948437/1‑148 (MQ=255)
                                                                     gcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTg                                                                           >  2:2461754/1‑148 (MQ=255)
                                                                     gcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTg                                                                           <  1:3416391/148‑1 (MQ=255)
                                                                      caATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGc                                                                          <  2:2333587/148‑1 (MQ=255)
                                                                          gTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGt                                                                      >  2:2843037/1‑148 (MQ=255)
                                                                            cAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGtat                                                                    <  2:3242337/148‑1 (MQ=255)
                                                                                ttGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACgg                                                                >  2:1104854/1‑148 (MQ=255)
                                                                                ttGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCAAAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACgg                                                                <  1:692478/148‑1 (MQ=255)
                                                                                     atCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCt                                                           >  2:2524145/1‑148 (MQ=255)
                                                                                          cAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCACCCGCTTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGt                                                      <  1:1627155/148‑1 (MQ=255)
                                                                                          cAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGt                                                      <  1:2527375/148‑1 (MQ=255)
                                                                                           aaaCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTa                                                     >  2:1730852/1‑148 (MQ=255)
                                                                                           aaaCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTa                                                     >  2:1730879/1‑148 (MQ=255)
                                                                                              cTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGtt                                                  >  1:2671051/1‑148 (MQ=255)
                                                                                              cTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGtt                                                  >  2:703365/1‑148 (MQ=255)
                                                                                              cTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGtt                                                  >  2:3592393/1‑148 (MQ=255)
                                                                                                 cgcgTTCATATCCCCGGCAAACTGAGTCGTATAAAAGTTATGAATTGTAGGTTGTATAACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGGTGCCAGTATACGGCATCTTCGGTAGTTGaa                                               <  2:2709207/148‑1 (MQ=255)
                                                                                                     tGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAaataa                                           <  2:3303426/148‑1 (MQ=255)
                                                                                                       cATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATg                                         >  2:355073/1‑148 (MQ=255)
                                                                                                           tCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACg                                     >  1:1721544/1‑148 (MQ=255)
                                                                                                           tCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACg                                     <  1:3314058/148‑1 (MQ=255)
                                                                                                            ccccGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACgg                                    <  1:291566/148‑1 (MQ=255)
                                                                                                            ccccGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACgg                                    >  1:1167931/1‑148 (MQ=255)
                                                                                                            ccccGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGGTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACgg                                    >  1:3013994/1‑148 (MQ=255)
                                                                                                               cGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGa                                 <  2:159524/148‑1 (MQ=255)
                                                                                                                ggCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGaa                                <  2:3900/148‑1 (MQ=255)
                                                                                                                    aaCTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGaa                            <  2:3713029/148‑1 (MQ=255)
                                                                                                                    aaCTGAGTCGTATAAAAGGTATGAAGTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGaa                            >  2:271844/1‑148 (MQ=255)
                                                                                                                     aCTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAac                           <  1:1916604/148‑1 (MQ=255)
                                                                                                                     aCTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAac                           <  1:90275/148‑1 (MQ=255)
                                                                                                                     aCTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAac                           >  1:3184503/1‑148 (MQ=255)
                                                                                                                       tGAGTCGTATAAAAGTTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAacac                         <  1:1409711/148‑1 (MQ=255)
                                                                                                                       tGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAacac                         >  2:2082689/1‑148 (MQ=255)
                                                                                                                         aGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCg                       >  2:863062/1‑148 (MQ=255)
                                                                                                                         aGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCg                       >  1:2118072/1‑148 (MQ=255)
                                                                                                                         aGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAAAACCg                       >  1:3030455/1‑148 (MQ=255)
                                                                                                                          gTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGt                      <  1:3271927/148‑1 (MQ=255)
                                                                                                                           tCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTa                     >  1:602479/1‑148 (MQ=255)
                                                                                                                            cGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTAc                    >  2:2307045/1‑148 (MQ=255)
                                                                                                                                    aGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTc            >  1:1074107/1‑148 (MQ=255)
                                                                                                                                     ggTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCa           <  1:740472/148‑1 (MQ=255)
                                                                                                                                     ggTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCa           <  1:50078/148‑1 (MQ=255)
                                                                                                                                      gTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCAc          >  1:1531899/1‑148 (MQ=255)
                                                                                                                                      gTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCAc          >  1:1211283/1‑148 (MQ=255)
                                                                                                                                       tATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACt         >  2:1680339/1‑148 (MQ=255)
                                                                                                                                        aTGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGATTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCAcat        >  2:2006361/1‑146 (MQ=255)
                                                                                                                                         tGAATTGTCGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTa       <  1:2165713/148‑1 (MQ=255)
                                                                                                                                              tgtaGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGTGGTTGCCAGTATACGTCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGc  <  2:1870605/148‑1 (MQ=255)
                                                                                                                                              tgtaGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGc  >  1:45927/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGC  >  CP009273/2918270‑2918559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑