Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,920,050 C→A 100% I128I (ATC→ATA)  ygdH → UPF0717 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,920,0500CA99.4% 586.6 / ‑4.2 179I128I (ATC→ATA) ygdHUPF0717 family protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (102/76);  total (103/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

AAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCC  >  CP009273/2919908‑2920192
                                                                                                                                              |                                                                                                                                              
aaGAGGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTaa                                                                                                                                           >  2:2647353/1‑148 (MQ=255)
aaGAGGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTaa                                                                                                                                           >  2:1370617/1‑148 (MQ=255)
aaGAGGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTaa                                                                                                                                           >  2:452274/1‑148 (MQ=255)
 agagGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACAtaactaac                                                                                                                                          <  1:3213183/148‑1 (MQ=255)
 agagGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACAtaactaac                                                                                                                                          <  1:1811819/148‑1 (MQ=255)
  gagGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAAcc                                                                                                                                         >  1:349922/1‑148 (MQ=255)
  gagGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAAcc                                                                                                                                         >  1:17168/1‑148 (MQ=255)
    ggCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTg                                                                                                                                       >  1:1010775/1‑148 (MQ=255)
     gCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTgg                                                                                                                                      >  1:2775705/1‑148 (MQ=255)
     gCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTgg                                                                                                                                      >  1:2935251/1‑148 (MQ=255)
     gCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTgg                                                                                                                                      >  1:207251/1‑148 (MQ=255)
     gCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTgg                                                                                                                                      >  1:1454363/1‑148 (MQ=255)
       ttttGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTc                                                                                                                                    <  2:2189739/148‑1 (MQ=255)
       ttttGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTc                                                                                                                                    >  2:2898194/1‑148 (MQ=255)
           gTCTATGGGCTAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTATCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCAGATAACTAACCTGGTCtttt                                                                                                                                <  1:894573/148‑1 (MQ=255)
           gTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCtttt                                                                                                                                <  1:140033/148‑1 (MQ=255)
             ctaTGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTcc                                                                                                                              >  1:3584730/3‑148 (MQ=255)
             ctaTGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTcc                                                                                                                              >  1:3585646/3‑148 (MQ=255)
               aTGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCAt                                                                                                                            <  1:2256555/148‑1 (MQ=255)
                tGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATc                                                                                                                           <  2:2683705/148‑1 (MQ=255)
                tGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATc                                                                                                                           <  2:36377/148‑1 (MQ=255)
                 gggCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCt                                                                                                                          <  2:2117290/148‑1 (MQ=255)
                  ggCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCtt                                                                                                                         >  1:1279978/1‑148 (MQ=255)
                  ggCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCtt                                                                                                                         >  2:2353159/1‑148 (MQ=255)
                    cGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGc                                                                                                                       >  2:3157832/1‑148 (MQ=255)
                    cGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGc                                                                                                                       >  2:2658661/1‑148 (MQ=255)
                       attattCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTa                                                                                                                    >  1:1711536/1‑148 (MQ=255)
                            tCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCt                                                                                                               >  2:448258/1‑148 (MQ=255)
                             cgcgCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTc                                                                                                              <  1:1600863/148‑1 (MQ=255)
                                 cTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTcgcgc                                                                                                          >  2:3584870/1‑148 (MQ=255)
                                   ttGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCgcgcgc                                                                                                        >  2:1072785/1‑148 (MQ=255)
                                   ttGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCgcgcgc                                                                                                        >  2:1663071/1‑148 (MQ=255)
                                   ttGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCgcgcgc                                                                                                        >  2:796176/1‑148 (MQ=255)
                                     gCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTg                                                                                                      <  2:3287426/148‑1 (MQ=255)
                                         gCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCAtg                                                                                                  <  1:3291857/148‑1 (MQ=255)
                                         gCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCAtg                                                                                                  <  1:687399/148‑1 (MQ=255)
                                          ccAACTTGTTCGCGGTCCTGCGTGACATACTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCAtgt                                                                                                 >  2:730239/1‑148 (MQ=255)
                                           cAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTGTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCAtgtg                                                                                                <  2:2783591/148‑1 (MQ=255)
                                            aaCTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTgg                                                                                               <  1:2019174/148‑1 (MQ=255)
                                            aaCTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTgg                                                                                               >  1:2415981/1‑148 (MQ=255)
                                              cTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGt                                                                                             >  1:1332717/1‑148 (MQ=255)
                                                  ttCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAg                                                                                         >  1:1905102/1‑148 (MQ=255)
                                                         tCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCaaa                                                                                  >  1:3139520/1‑148 (MQ=255)
                                                          ccTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAat                                                                                 <  2:1925475/148‑1 (MQ=255)
                                                           cTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAata                                                                                <  1:2487863/148‑1 (MQ=255)
                                                                tGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtggtg                                                                           >  2:1398715/1‑148 (MQ=255)
                                                                tGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtggtg                                                                           >  1:3317587/1‑148 (MQ=255)
                                                                tGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtggtg                                                                           >  1:3317579/1‑148 (MQ=255)
                                                                  aCATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATggtggt                                                                         <  1:1917957/148‑1 (MQ=255)
                                                                        tctTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTctgctg                                                                   <  2:1870920/148‑1 (MQ=255)
                                                                        tctTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTctgctg                                                                   <  2:3760124/148‑1 (MQ=255)
                                                                        tctTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTctgctg                                                                   <  2:600777/148‑1 (MQ=255)
                                                                         ctTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTgg                                                                  >  2:1529805/1‑148 (MQ=255)
                                                                           tCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTgggg                                                                <  2:3769505/148‑1 (MQ=255)
                                                                            cGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGc                                                               <  2:1594246/148‑1 (MQ=255)
                                                                            cGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGc                                                               <  2:3492662/148‑1 (MQ=255)
                                                                                    ggCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTc                                                       >  1:3299902/1‑148 (MQ=255)
                                                                                     gCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCa                                                      <  1:3170197/148‑1 (MQ=255)
                                                                                     gCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCa                                                      <  1:2289160/148‑1 (MQ=255)
                                                                                      cAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCaa                                                     <  2:2481472/148‑1 (MQ=255)
                                                                                       aaaTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAAt                                                    >  1:2737665/1‑148 (MQ=255)
                                                                                       aaaTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAAt                                                    >  1:2737727/1‑148 (MQ=255)
                                                                                         aTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTa                                                  >  2:1594858/1‑148 (MQ=255)
                                                                                             aTAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGa                                              >  1:626113/1‑148 (MQ=255)
                                                                                              tAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaa                                             >  1:957145/1‑148 (MQ=255)
                                                                                               aaCACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaaa                                            <  1:3753039/148‑1 (MQ=255)
                                                                                               aaCACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaaa                                            <  2:207251/148‑1 (MQ=255)
                                                                                               aaCACCGTTAGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaaa                                            <  1:478326/148‑1 (MQ=255)
                                                                                                acacCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaaaa                                           <  2:1251411/148‑1 (MQ=255)
                                                                                                 cacCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAAc                                          >  1:2339310/1‑148 (MQ=255)
                                                                                                 cacCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAAc                                          >  2:421171/1‑148 (MQ=255)
                                                                                                  acCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACg                                         <  1:2658661/148‑1 (MQ=255)
                                                                                                  acCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACg                                         <  1:3157832/148‑1 (MQ=255)
                                                                                                     gttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGt                                      <  2:1856489/148‑1 (MQ=255)
                                                                                                      ttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTa                                     <  1:2653743/148‑1 (MQ=255)
                                                                                                      ttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTa                                     <  2:1713455/148‑1 (MQ=255)
                                                                                                       tcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTAt                                    >  1:2698591/1‑148 (MQ=255)
                                                                                                       tcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTAt                                    >  1:3392371/1‑148 (MQ=255)
                                                                                                          ttttCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAACACGAGTATTTg                                 >  2:3047907/1‑148 (MQ=255)
                                                                                                          ttttCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTg                                 >  2:2648628/1‑148 (MQ=255)
                                                                                                              cccAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATg                             <  1:1349629/148‑1 (MQ=255)
                                                                                                               ccAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGc                            <  1:2345183/148‑1 (MQ=255)
                                                                                                                cAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGcc                           <  2:482612/148‑1 (MQ=255)
                                                                                                                cAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGcc                           >  1:1085063/1‑148 (MQ=255)
                                                                                                                cAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGcc                           >  1:412470/1‑148 (MQ=255)
                                                                                                                 aaCCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGccc                          >  1:1467915/1‑148 (MQ=255)
                                                                                                                  aCCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcg                         <  2:3584730/148‑1 (MQ=255)
                                                                                                                  aCCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcg                         >  2:1496769/1‑148 (MQ=255)
                                                                                                                      gAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgc                     >  2:103657/1‑148 (MQ=255)
                                                                                                                       aaTCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcg                    <  2:1983292/148‑1 (MQ=255)
                                                                                                                       aaTCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcg                    <  2:1308555/148‑1 (MQ=255)
                                                                                                                            cGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGa               >  2:2258381/1‑148 (MQ=255)
                                                                                                                            cGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGa               >  1:3033890/1‑148 (MQ=255)
                                                                                                                                 aCTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCa          >  2:3106922/1‑148 (MQ=255)
                                                                                                                                    ccGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCt       >  2:3344357/1‑148 (MQ=255)
                                                                                                                                    ccGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCt       >  2:1442158/1‑148 (MQ=255)
                                                                                                                                     cGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTg      >  2:2530460/1‑148 (MQ=255)
                                                                                                                                       tCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTggg    >  2:1733772/1‑148 (MQ=255)
                                                                                                                                        ccACATAACTAACCTGGTCTTGTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGc   <  2:601030/148‑1 (MQ=255)
                                                                                                                                         cacaTAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGcc  >  1:2857350/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                              
AAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCC  >  CP009273/2919908‑2920192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑