Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,921,245 G→T 100% intergenic (+214/‑343) ygdH → / → sdaC UPF0717 family protein/putative serine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,921,2450GT99.7% 935.6 / ‑4.5 283intergenic (+214/‑343)ygdH/sdaCUPF0717 family protein/putative serine transporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (153/129);  total (154/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 285 total aligned reads displayed.

ATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAATCTATCTT  >  CP009273/2921102‑2921392
                                                                                                                                               |                                                                                                                                                   
  ttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAAc                                                                                                                                               <  1:3197629/148‑1 (MQ=255)
     ttATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTaa                                                                                                                                            >  1:2640948/1‑148 (MQ=255)
      tataTGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTaaa                                                                                                                                           <  1:3333318/148‑1 (MQ=255)
       atatGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTaaaa                                                                                                                                          >  2:2790210/1‑148 (MQ=255)
         atGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGt                                                                                                                                        >  1:969618/1‑148 (MQ=255)
         atGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGt                                                                                                                                        >  1:3422247/1‑148 (MQ=255)
          tGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTa                                                                                                                                       <  2:2019290/148‑1 (MQ=255)
          tGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTa                                                                                                                                       >  1:3025848/1‑148 (MQ=255)
             aTGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTg                                                                                                                                    <  1:3179509/148‑1 (MQ=255)
             aTGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTg                                                                                                                                    >  2:657646/1‑148 (MQ=255)
             aTGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTg                                                                                                                                    >  1:1319965/1‑148 (MQ=255)
               gTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTCTTGAt                                                                                                                                  >  2:2486175/1‑148 (MQ=255)
               gTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGAt                                                                                                                                  <  2:2722686/148‑1 (MQ=255)
                      accaccCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCa                                                                                                                           >  2:1093082/1‑148 (MQ=255)
                          cccTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTc                                                                                                                       >  1:2586321/1‑148 (MQ=255)
                          cccTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTc                                                                                                                       >  2:164428/1‑148 (MQ=255)
                           ccTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCa                                                                                                                      >  1:3616150/1‑148 (MQ=255)
                            cTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCaa                                                                                                                     <  2:3682618/148‑1 (MQ=255)
                             tCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAAc                                                                                                                    >  2:1412128/1‑148 (MQ=255)
                             tCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATAATCGCCATTATTAACTAAAAGTATTTATTTTTTCAGTTCatc                                                                                                                    >  1:1910114/1‑146 (MQ=255)
                              cACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAAcc                                                                                                                   <  2:1871519/148‑1 (MQ=255)
                              cACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAAcc                                                                                                                   <  1:1004939/148‑1 (MQ=255)
                              cACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAAcc                                                                                                                   <  1:3754530/148‑1 (MQ=255)
                                     cAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACatat                                                                                                            >  2:2871746/1‑148 (MQ=255)
                                     cAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACatat                                                                                                            >  2:1252127/1‑148 (MQ=255)
                                     cAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACatat                                                                                                            <  2:1044656/148‑1 (MQ=255)
                                      aaaTATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACatata                                                                                                           <  1:2906944/148‑1 (MQ=255)
                                        atatCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTCCATATAtt                                                                                                         >  1:3695164/1‑148 (MQ=255)
                                        atatCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATAtt                                                                                                         >  1:1200724/1‑148 (MQ=255)
                                        atatCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATAtt                                                                                                         >  2:2150415/1‑148 (MQ=255)
                                         tataATCACAGTTAATATGTAATACAATTTATGTTGCAACGAAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTg                                                                                                        <  1:1800579/148‑1 (MQ=255)
                                         tatCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTg                                                                                                        >  2:911232/1‑148 (MQ=255)
                                          atcatcACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTgc                                                                                                       >  2:738548/1‑148 (MQ=255)
                                          atcatcACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTgc                                                                                                       >  2:2695072/1‑148 (MQ=255)
                                             atcaCAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTgcgcg                                                                                                    >  1:570002/1‑148 (MQ=255)
                                              tcaCAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTACAAGTATTGATTTTTTCGGGTCAACCTACATATATTgcgcgc                                                                                                   >  2:1002389/1‑148 (MQ=255)
                                               cacaGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGcc                                                                                                  >  2:170360/1‑148 (MQ=255)
                                               cacaGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGcc                                                                                                  <  2:66406/148‑1 (MQ=255)
                                                  aGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCg                                                                                               >  2:1985464/1‑148 (MQ=255)
                                                   gTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCTCGCCCCgg                                                                                              >  2:3399505/1‑148 (MQ=255)
                                                    ttAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGga                                                                                             <  1:2606214/148‑1 (MQ=255)
                                                     tAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGgaa                                                                                            >  2:2667255/1‑148 (MQ=255)
                                                         atGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGaagaag                                                                                        >  2:30731/1‑148 (MQ=255)
                                                          tGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGTGCGCCCCGGAAGAAGt                                                                                       >  2:460894/1‑148 (MQ=255)
                                                           gTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTc                                                                                      >  2:1481529/1‑148 (MQ=255)
                                                               tACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGAt                                                                                  >  2:2817077/1‑148 (MQ=255)
                                                               tACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGAt                                                                                  >  2:3437595/1‑148 (MQ=255)
                                                                aCAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATg                                                                                 >  1:1256148/1‑148 (MQ=255)
                                                                 cAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGt                                                                                <  1:1429560/148‑1 (MQ=255)
                                                                   aTTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACGTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCg                                                                              >  1:3465289/1‑148 (MQ=255)
                                                                   aTTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCg                                                                              >  1:112850/1‑148 (MQ=255)
                                                                   aTTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCCTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCg                                                                              >  1:3589540/1‑148 (MQ=255)
                                                                     ttATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGtt                                                                            <  1:2790210/148‑1 (MQ=255)
                                                                         gTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAAt                                                                        >  1:3119597/1‑148 (MQ=255)
                                                                          ttGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTCCATATATTGCGCGCCCCGGAAGAAGTCAGTTGTCGTTTAATg                                                                       >  1:2433708/1‑148 (MQ=255)
                                                                                 gCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAat                                                                <  1:663629/148‑1 (MQ=255)
                                                                                  cAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAata                                                               >  2:3635471/1‑148 (MQ=255)
                                                                                   aaaCGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAatat                                                              <  2:139657/148‑1 (MQ=255)
                                                                                      cGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGc                                                           >  2:1798125/1‑148 (MQ=255)
                                                                                        tttCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGccc                                                         <  1:1916422/148‑1 (MQ=255)
                                                                                           cccTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTa                                                      >  2:3767427/1‑148 (MQ=255)
                                                                                            ccTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCTCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTTCCCTTaa                                                     >  2:1676100/1‑148 (MQ=255)
                                                                                            ccTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTaa                                                     <  1:1452820/148‑1 (MQ=255)
                                                                                            ccTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTaa                                                     <  1:1783234/148‑1 (MQ=255)
                                                                                             cTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATAGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTaaa                                                    >  1:220972/1‑148 (MQ=255)
                                                                                                 tttCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGAGGTCGTTTAATGGGCAAATATTGCCCTTAAATtct                                                >  1:525650/1‑148 (MQ=255)
                                                                                                  ttCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATtctc                                               <  2:294274/148‑1 (MQ=255)
                                                                                                    cATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCtt                                             >  1:2885808/1‑148 (MQ=255)
                                                                                                      tAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCtttt                                           >  1:1519864/1‑148 (MQ=255)
                                                                                                        aaCCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTAc                                         <  1:3633422/148‑1 (MQ=255)
                                                                                                          ccGTATATTATCGCTATCAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATATTTTCAGTTCAACCTACACATATTGCGCGCCCCGGAAGAAGTCAGATGCCGATTAATGGGCAAAAAGTGCCCTTAAATTCTCTTTTAcat                                       <  2:1589297/148‑3 (MQ=255)
                                                                                                           cGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACttt                                      <  1:2329565/148‑1 (MQ=255)
                                                                                                           cGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACttt                                      <  1:2354210/148‑1 (MQ=255)
                                                                                                             tATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTg                                    <  2:3662961/148‑1 (MQ=255)
                                                                                                              aTTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGa                                   <  1:911247/148‑1 (MQ=255)
                                                                                                               ttttATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGAt                                  <  1:1975680/148‑1 (MQ=255)
                                                                                                                  tATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTa                               <  2:1536131/148‑1 (MQ=255)
                                                                                                                   aTCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTAc                              >  1:3369708/1‑148 (MQ=255)
                                                                                                                      gCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACaga                           <  1:2695144/148‑1 (MQ=255)
                                                                                                                      gCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACaga                           <  1:738548/148‑1 (MQ=255)
                                                                                                                       cTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACagag                          <  2:1924599/148‑1 (MQ=255)
                                                                                                                        tATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGt                         >  1:3396582/1‑148 (MQ=255)
                                                                                                                         aTGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTa                        <  1:2107980/148‑1 (MQ=255)
                                                                                                                         aTGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTa                        <  2:3299868/148‑1 (MQ=255)
                                                                                                                          tGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTaa                       <  1:164428/148‑1 (MQ=255)
                                                                                                                            aaaaaGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAg                     <  2:2277750/148‑1 (MQ=255)
                                                                                                                            aaaaaGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAg                     <  1:2014283/148‑1 (MQ=255)
                                                                                                                              aaaGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCg                   >  2:2570571/1‑148 (MQ=255)
                                                                                                                               aaGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGt                  >  2:2376203/1‑148 (MQ=255)
                                                                                                                               aaGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTCCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATTTTGCCCTTAAATTCTCTTTTAATATTGATTTACACAGACAAGCGt                  >  2:3715427/1‑148 (MQ=255)
                                                                                                                                     ttATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGAt            >  2:2038228/1‑148 (MQ=255)
                                                                                                                                      tATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATa           >  1:2083644/1‑148 (MQ=255)
                                                                                                                                      tATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATa           <  1:456472/148‑1 (MQ=255)
                                                                                                                                      tATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATa           >  1:1455212/1‑148 (MQ=255)
                                                                                                                                      tATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATa           <  1:865996/148‑1 (MQ=255)
                                                                                                                                        tCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAat         <  2:384720/148‑1 (MQ=255)
                                                                                                                                         cGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatc        >  2:3552343/1‑148 (MQ=255)
                                                                                                                                         cGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatc        >  1:2271161/1‑148 (MQ=255)
                                                                                                                                         cGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatc        <  1:3788514/148‑1 (MQ=255)
                                                                                                                                           ccATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAATGCGTTGGGATAatcta      >  1:3087787/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
ATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAATCTATCTT  >  CP009273/2921102‑2921392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑