Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,932,835 C→T 100% R37C (CGC→TGC)  fucR → l‑fucose operon activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,932,8350CT99.5% 708.9 / ‑4.5 213R37C (CGC→TGC) fucRl‑fucose operon activator
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (109/103);  total (109/104)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

TGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATCGCGC  >  CP009273/2932691‑2932979
                                                                                                                                                |                                                                                                                                                
tGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTtgcg                                                                                                                                               >  1:2491576/1‑148 (MQ=255)
  aCGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGAt                                                                                                                                             >  1:2105750/1‑148 (MQ=255)
      gggcggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCa                                                                                                                                         <  2:1111255/148‑1 (MQ=255)
       ggcggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCaa                                                                                                                                        >  1:3423995/1‑148 (MQ=255)
       ggcggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCaa                                                                                                                                        <  2:1987169/148‑1 (MQ=255)
       ggcggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCaa                                                                                                                                        >  1:2327443/1‑148 (MQ=255)
         cggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATg                                                                                                                                      <  1:1765046/148‑1 (MQ=255)
          ggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGa                                                                                                                                     <  1:1811472/148‑1 (MQ=255)
            ttATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAAt                                                                                                                                   >  1:734382/1‑148 (MQ=255)
              aTCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTa                                                                                                                                 <  2:1586135/148‑1 (MQ=255)
              aTCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTa                                                                                                                                 <  2:56860/148‑1 (MQ=255)
                cGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACa                                                                                                                               >  2:2453587/1‑148 (MQ=255)
                 taaTGATGGGTTGAAAAATATGAAAGCGGCACGCCATCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAg                                                                                                                              <  1:1654269/147‑1 (MQ=255)
                    tgatgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACg                                                                                                                           >  2:674168/1‑148 (MQ=255)
                      atgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCa                                                                                                                         <  2:774478/148‑1 (MQ=255)
                        ggggTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGAAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAgg                                                                                                                       >  2:2162954/1‑148 (MQ=255)
                               aaaaTATGAACGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAAt                                                                                                                <  1:5391/148‑1 (MQ=255)
                                aaaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAAtt                                                                                                               >  1:3778789/1‑148 (MQ=255)
                                aaaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAAtt                                                                                                               <  1:50233/148‑1 (MQ=255)
                                aaaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAAtt                                                                                                               <  2:2375179/148‑1 (MQ=255)
                                aaaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAAtt                                                                                                               >  1:2445519/1‑148 (MQ=255)
                                  atatGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCt                                                                                                             >  1:2567670/1‑148 (MQ=255)
                                   tatGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTg                                                                                                            >  2:3107882/1‑148 (MQ=255)
                                   tatGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTg                                                                                                            <  1:144109/148‑1 (MQ=255)
                                   tatGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTg                                                                                                            <  1:119976/148‑1 (MQ=255)
                                   tatGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTg                                                                                                            >  2:1201847/1‑148 (MQ=255)
                                       aaaGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCa                                                                                                        >  1:2718597/1‑148 (MQ=255)
                                          gCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATc                                                                                                     <  1:1419042/148‑1 (MQ=255)
                                           cGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCa                                                                                                    <  2:1721329/148‑1 (MQ=255)
                                            ggCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCCATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCAt                                                                                                   <  1:863861/148‑1 (MQ=255)
                                            ggCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCAt                                                                                                   <  1:3046253/148‑1 (MQ=255)
                                              cACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATgg                                                                                                 <  2:2327443/148‑1 (MQ=255)
                                                cGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGAc                                                                                               >  2:282255/1‑148 (MQ=255)
                                                   cAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACTGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAACCCATTCGTTGCGATCTCCATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGAcgcg                                                                                            <  1:3176418/148‑1 (MQ=255)
                                                   cAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGAcgcg                                                                                            >  1:1676014/1‑148 (MQ=255)
                                                   cAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGAcgcg                                                                                            <  2:437706/148‑1 (MQ=255)
                                                    aGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAACCCATTCGTTGCGATCTCACTGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACgcgc                                                                                           <  1:1681700/148‑1 (MQ=255)
                                                        aGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTaaa                                                                                       <  2:1805585/148‑1 (MQ=255)
                                                         gCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAat                                                                                      <  2:2039358/148‑1 (MQ=255)
                                                         gCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAat                                                                                      <  2:1247554/148‑1 (MQ=255)
                                                         gCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAat                                                                                      >  2:1933501/1‑148 (MQ=255)
                                                          cGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGTATTACAGACGCAGGGTAAAATTCTGCGCAATCAGGGACGCGCGAAata                                                                                     >  1:3714143/1‑148 (MQ=255)
                                                          cGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAata                                                                                     >  1:3715006/1‑148 (MQ=255)
                                                           gATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAatat                                                                                    <  2:885582/148‑1 (MQ=255)
                                                             tAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAatatat                                                                                  <  2:2975189/148‑1 (MQ=255)
                                                               gTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATcc                                                                                <  1:1003586/148‑1 (MQ=255)
                                                                tCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCa                                                                               >  1:394942/1‑148 (MQ=255)
                                                                    ccTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAACCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGt                                                                           <  2:915228/148‑1 (MQ=255)
                                                                    ccTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGt                                                                           >  2:1764391/1‑148 (MQ=255)
                                                                    ccTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGt                                                                           >  2:3486044/1‑148 (MQ=255)
                                                                         tgctgAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCaaaa                                                                      <  1:3537593/148‑1 (MQ=255)
                                                                               aCCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGa                                                                <  1:2816917/148‑1 (MQ=255)
                                                                                    aCCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTg                                                           >  1:619985/1‑148 (MQ=255)
                                                                                         ccTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGAt                                                      >  1:3339224/1‑148 (MQ=255)
                                                                                            gACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATccc                                                   <  2:1460142/148‑1 (MQ=255)
                                                                                               cACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCttt                                                >  1:878437/1‑148 (MQ=255)
                                                                                               cACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCttt                                                >  1:97792/1‑148 (MQ=255)
                                                                                                  ggAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTcac                                             <  1:966262/148‑1 (MQ=255)
                                                                                                    aaGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACAt                                           >  2:3555422/1‑148 (MQ=255)
                                                                                                     aGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATc                                          <  1:92640/148‑1 (MQ=255)
                                                                                                      gCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCa                                         >  2:1166678/1‑148 (MQ=255)
                                                                                                      gCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCa                                         >  1:2239256/1‑148 (MQ=255)
                                                                                                       ctctctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAg                                        >  2:1646117/1‑148 (MQ=255)
                                                                                                           ctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTg                                    >  1:3754408/1‑148 (MQ=255)
                                                                                                            tctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGa                                   >  2:723238/1‑148 (MQ=255)
                                                                                                              tGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGaaa                                 <  2:3552670/148‑1 (MQ=255)
                                                                                                                  cAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGcc                             >  1:2021078/1‑148 (MQ=255)
                                                                                                                    gCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCCAGGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAt                           <  1:47167/148‑1 (MQ=255)
                                                                                                                     cTACAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAtt                          <  2:2336257/148‑1 (MQ=255)
                                                                                                                     cTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAtt                          <  1:817934/148‑1 (MQ=255)
                                                                                                                     cTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGAAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAtt                          >  1:862732/1‑148 (MQ=255)
                                                                                                                      tAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTa                         <  1:132056/148‑1 (MQ=255)
                                                                                                                      tAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTa                         <  2:2115151/148‑1 (MQ=255)
                                                                                                                       aaaGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTAt                        >  1:19834/1‑148 (MQ=255)
                                                                                                                        aaGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATg                       <  2:3089063/148‑1 (MQ=255)
                                                                                                                           gTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATgcgc                    <  1:2129813/148‑1 (MQ=255)
                                                                                                                            tCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCa                   <  1:3655012/148‑1 (MQ=255)
                                                                                                                               gTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATaa                <  2:394942/148‑1 (MQ=255)
                                                                                                                                 aaaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAg              >  2:589530/1‑148 (MQ=255)
                                                                                                                                  aaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGc             <  2:3180637/148‑1 (MQ=255)
                                                                                                                                   aGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCa            >  1:1103550/1‑148 (MQ=255)
                                                                                                                                    gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAg           <  2:2491576/148‑1 (MQ=255)
                                                                                                                                    gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAg           >  2:1167325/1‑148 (MQ=255)
                                                                                                                                    gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAg           >  2:1211754/1‑148 (MQ=255)
                                                                                                                                    gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAg           <  1:1335536/148‑1 (MQ=255)
                                                                                                                                    gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAg           >  2:749542/1‑148 (MQ=255)
                                                                                                                                    gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAg           >  2:1212641/1‑148 (MQ=255)
                                                                                                                                     aaaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGa          <  2:2105750/148‑1 (MQ=255)
                                                                                                                                     aaaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGa          <  2:1676097/148‑1 (MQ=255)
                                                                                                                                     aaaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGa          >  1:1435617/1‑148 (MQ=255)
                                                                                                                                       aCCATTCGTTTCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCGTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGata        <  2:1382343/148‑1 (MQ=255)
                                                                                                                                       aCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGata        <  2:3378547/148‑1 (MQ=255)
                                                                                                                                       aCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGata        >  1:3165223/1‑148 (MQ=255)
                                                                                                                                       aCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGata        <  1:2948378/148‑1 (MQ=255)
                                                                                                                                        ccATTCGTTGCGATTTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGatat       >  1:3256901/1‑148 (MQ=255)
                                                                                                                                          aTTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcg     >  1:3798133/1‑148 (MQ=255)
                                                                                                                                          aTTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcg     >  1:1978502/1‑148 (MQ=255)
                                                                                                                                          aTTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcg     >  2:340111/1‑148 (MQ=255)
                                                                                                                                            tcgttgCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgcg   <  1:3263100/148‑1 (MQ=255)
                                                                                                                                            tcgttgCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgcg   <  1:3486044/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATCGCGC  >  CP009273/2932691‑2932979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑