BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,950,431	T→A	22.0%	N605Y (AAT→TAT)	ptrA ←	protease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,950,431	0	T	A	22.0%	490.5 / 145.5	227	N605Y (AAT→TAT)	ptrA	protease III
Reads supporting (aligned to +/- strand): ref base T (88/89); new base A (25/25); total (113/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 235 total aligned reads displayed.

ACGCTTTACCCTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGAT  >  CP009273/2950284‑2950577

  gCTTTACCCTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTaa                                                                                                                                                  <  2:2290173/148‑1 (MQ=255)

   cTTTACCCTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAAc                                                                                                                                                 <  2:184366/148‑1 (MQ=255)

    tttACCCTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAAcc                                                                                                                                                >  1:143026/1‑148 (MQ=255)

     ttACCCTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCa                                                                                                                                               >  2:224413/1‑148 (MQ=255)

     ttACCCTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCa                                                                                                                                               >  2:1183164/1‑148 (MQ=255)

      tACCCTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCAt                                                                                                                                              >  1:1194611/1‑148 (MQ=255)

      tACCCTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCAt                                                                                                                                              >  1:3100788/1‑148 (MQ=255)

       aCCCTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATa                                                                                                                                             <  2:2483989/148‑1 (MQ=255)

        cccTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATaa                                                                                                                                            <  1:165801/148‑1 (MQ=255)

        cccTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATaa                                                                                                                                            <  1:60974/148‑1 (MQ=255)

            tttCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGcc                                                                                                                                        <  1:2935047/148‑1 (MQ=255)

              tCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCgt                                                                                                                                      <  1:136090/148‑1 (MQ=255)

               cTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCgtt                                                                                                                                     <  1:3397256/148‑1 (MQ=255)

                tGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCgttg                                                                                                                                    <  2:1808525/148‑1 (MQ=255)

                 gCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCgttgt                                                                                                                                   <  2:3622907/148‑1 (MQ=255)

                  cGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCgttgtt                                                                                                                                  <  1:2393666/148‑1 (MQ=255)

                    gAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAg                                                                                                                                <  1:3602953/148‑1 (MQ=255)

                    gAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTGGTTAg                                                                                                                                >  1:1324670/1‑148 (MQ=255)

                    gAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAg                                                                                                                                >  1:2022845/1‑148 (MQ=255)

                     aaTCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAAACATAAGGCCGTTGTTAGc                                                                                                                               >  2:3289938/1‑148 (MQ=255)

                       tCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGt                                                                                                                             >  1:2531537/1‑148 (MQ=255)

                        ccATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGtt                                                                                                                            >  2:2089213/1‑148 (MQ=255)

                        ccATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGtt                                                                                                                            >  2:2089223/1‑148 (MQ=255)

                           tcatcTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGACCAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTtggt                                                                                                                         >  1:1591406/1‑148 (MQ=255)

                           tcatcTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTtggt                                                                                                                         >  2:1568375/1‑148 (MQ=255)

                            catcTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATCAGGCCGTTGTTAGCGTtggtg                                                                                                                        >  2:2874308/1‑148 (MQ=255)

                                tGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGaaa                                                                                                                    <  1:2553022/148‑1 (MQ=255)

                                 ggTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGaaaa                                                                                                                   <  1:3186242/148‑1 (MQ=255)

                                     ataCCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACtt                                                                                                               <  1:2012384/148‑1 (MQ=255)

                                     ataCCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACtt                                                                                                               <  1:516329/148‑1 (MQ=255)

                                     ataCCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACtt                                                                                                               <  2:3093203/148‑1 (MQ=255)

                                        ccAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATg                                                                                                            <  2:343470/148‑1 (MQ=255)

                                         cAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGc                                                                                                           >  2:1284154/1‑148 (MQ=255)

                                          aGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGcc                                                                                                          >  1:879851/1‑148 (MQ=255)

                                            gACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGccac                                                                                                        >  2:1870356/1‑148 (MQ=255)

                                            gACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGccac                                                                                                        <  1:1294919/148‑1 (MQ=255)

                                                tCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAAc                                                                                                    >  1:2305847/1‑148 (MQ=255)

                                                tCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAAc                                                                                                    >  1:2305092/1‑148 (MQ=255)

                                                  gCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCg                                                                                                  <  1:3356766/148‑1 (MQ=255)

                                                      gCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGc                                                                                              <  2:3295099/148‑1 (MQ=255)

                                                       cTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGcc                                                                                             >  2:1764897/1‑148 (MQ=255)

                                                       cTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGcc                                                                                             <  2:2134186/148‑1 (MQ=255)

                                                           aGCTGAACTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGt                                                                                         <  2:3310700/148‑1 (MQ=255)

                                                            gCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGtt                                                                                        >  1:391131/1‑148 (MQ=255)

                                                               gATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCt                                                                                     <  1:3162226/148‑1 (MQ=255)

                                                               gATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGGTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCt                                                                                     >  2:617601/1‑148 (MQ=255)

                                                                aTCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCtt                                                                                    >  1:2906748/1‑148 (MQ=255)

                                                                 tCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTa                                                                                   >  1:3457423/1‑148 (MQ=255)

                                                                  cTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTaa                                                                                  >  2:1623953/1‑148 (MQ=255)

                                                                  cTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTaa                                                                                  >  2:1623843/1‑148 (MQ=255)

                                                                       gTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGAt                                                                             <  1:3081073/148‑1 (MQ=255)

                                                                           cGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAg                                                                         <  1:889657/148‑1 (MQ=255)

                                                                                   cTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGcc                                                                 >  1:3534501/1‑148 (MQ=255)

                                                                                   cTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGcc                                                                 >  2:2457833/1‑148 (MQ=255)

                                                                                    tAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGccc                                                                <  2:853935/148‑1 (MQ=255)

                                                                                       aGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGc                                                             >  2:1264010/1‑148 (MQ=255)

                                                                                           ccccTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCgaga                                                         <  1:1039861/148‑1 (MQ=255)

                                                                                             ccTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATa                                                       <  1:2739722/148‑1 (MQ=255)

                                                                                             ccTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATa                                                       <  2:1071486/148‑1 (MQ=255)

                                                                                              cTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATaa                                                      <  2:1933479/148‑1 (MQ=255)

                                                                                              cTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATaa                                                      <  1:1870356/148‑1 (MQ=255)

                                                                                              cTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATaa                                                      >  2:3666630/1‑148 (MQ=255)

                                                                                                cGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATc                                                    <  2:676596/148‑1 (MQ=255)

                                                                                                cGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATc                                                    <  2:2051953/148‑1 (MQ=255)

                                                                                                 gAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCa                                                   <  2:2156242/148‑1 (MQ=255)

                                                                                                   gCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCAtt                                                 <  2:2818317/148‑1 (MQ=255)

                                                                                                   gCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCAtt                                                 <  2:2308231/148‑1 (MQ=255)

                                                                                                     aaTGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGa                                               <  1:736469/148‑1 (MQ=255)

                                                                                                       tGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAgc                                             >  1:2252456/1‑148 (MQ=255)

                                                                                                         ccTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAgcgc                                           <  2:2163698/148‑1 (MQ=255)

                                                                                                             gAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAAc                                       >  2:953556/1‑148 (MQ=255)

                                                                                                             gAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAAc                                       >  2:953648/1‑148 (MQ=255)

                                                                                                                cAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATc                                    >  1:686370/1‑148 (MQ=255)

                                                                                                                  gCTGCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCAc                                  <  2:867719/148‑1 (MQ=255)

                                                                                                                     gCGGCAGACGCTGGGTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAAGATCACCTg                               <  1:2137179/148‑1 (MQ=255)

                                                                                                                       ggCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGAt                             <  2:3156854/148‑1 (MQ=255)

                                                                                                                           gACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCg                         >  2:1914584/1‑148 (MQ=255)

                                                                                                                            aCGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCAATGAGCGCAAACATCACCTGATTGCgg                        >  1:1180740/1‑148 (MQ=255)

                                                                                                                                  ggTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTg                  >  2:2651987/1‑148 (MQ=255)

                                                                                                                                  ggTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGAGCTg                  >  1:2193476/1‑148 (MQ=255)

                                                                                                                                  ggTGTAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTg                  <  1:2316562/148‑1 (MQ=255)

                                                                                                                                   gtgtAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGt                 <  2:3500249/148‑1 (MQ=255)

                                                                                                                                   gtgtAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGt                 <  1:3118288/148‑1 (MQ=255)

                                                                                                                                   gtgtAACCATTAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGCAAAAACTTATGGCAGGTACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGt                 >  1:3746744/1‑148 (MQ=255)

                                                                                                                                    tgtAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTc                <  1:3313814/148‑1 (MQ=255)

                                                                                                                                    tgtAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTc                >  1:1854811/1‑148 (MQ=255)

                                                                                                                                     gtAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTcc               >  2:1884686/1‑148 (MQ=255)

                                                                                                                                      tAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCa              >  1:3628446/1‑148 (MQ=255)

                                                                                                                                        aCCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATg            >  2:3784187/1‑148 (MQ=255)

                                                                                                                                         ccATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATgg           >  1:1553451/1‑148 (MQ=255)

                                                                                                                                           aTTAGCATAAACCATACGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCt         <  2:3053128/148‑1 (MQ=255)

                                                                                                                                            ttAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCtt        >  2:1092692/1‑148 (MQ=255)

                                                                                                                                            ttAGCATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCtt        <  2:400433/148‑1 (MQ=255)

                                                                                                                                              aGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTc      >  2:628545/1‑148 (MQ=255)

                                                                                                                                                cATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCgg    >  2:3302710/1‑148 (MQ=255)

                                                                                                                                                cATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCgg    <  1:242002/148‑1 (MQ=255)

                                                                                                                                                cATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCgg    >  2:2025921/1‑148 (MQ=255)

                                                                                                                                                cATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCgg    <  1:263052/148‑1 (MQ=255)

                                                                                                                                                cATAAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCgg    <  1:2068387/148‑1 (MQ=255)

                                                                                                                                                  ttAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGAt  >  1:600416/1‑148 (MQ=255)

ACGCTTTACCCTTTTCTGCGGAATCCATCATCTGGTTATACCAGGACTTCGCCTGCTCAAGCTGATCTTCCGTAGCGGTATAGCTAAAGTACCCCTCGAGCAATGCCTGGAACAGCTGCGGCAGACGCTGGGTGTAACCATTAGCATTAACCATAAGGCCGTTGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGAT  >  CP009273/2950284‑2950577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`