Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,968,152 G→T 10.2% R408S (CGC→AGC)  aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,968,1520GT10.2% 878.6 / 46.4 260R408S (CGC→AGC) aasfused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base G (108/125);  new base T (16/11);  total (124/136)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

TGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTG  >  CP009273/2968005‑2968299
                                                                                                                                                   |                                                                                                                                                   
tGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCg                                                                                                                                                     <  1:3590903/148‑1 (MQ=255)
 gCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGa                                                                                                                                                    <  2:3274078/148‑1 (MQ=255)
  cAACTGCGGTCATACACCAACTCCGGCACATTGCGGTAATGCAGCGGGCTTGTATAAATGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGAt                                                                                                                                                   <  1:3035025/148‑1 (MQ=255)
     cTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGt                                                                                                                                                >  1:2866519/1‑148 (MQ=255)
     cTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGt                                                                                                                                                <  2:3310024/148‑1 (MQ=255)
       gCGGTCATACACCAACTCCGGCACAATGCTGTATTGCAGCGGGCTTGGATAAAGGAACACTTCTGCACATGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGTGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTtg                                                                                                                                              <  2:387169/148‑1 (MQ=255)
              tACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGa                                                                                                                                       >  1:2445001/1‑148 (MQ=255)
              tACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGa                                                                                                                                       <  2:2162690/148‑1 (MQ=255)
              tACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGa                                                                                                                                       >  1:1943194/1‑148 (MQ=255)
               acacCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCTATCGTTGGTGGTGaa                                                                                                                                      <  1:3698382/148‑1 (MQ=255)
                cacCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAACGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAg                                                                                                                                     <  1:323006/148‑1 (MQ=255)
                cacCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAg                                                                                                                                     <  1:498492/148‑1 (MQ=255)
                cacCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAg                                                                                                                                     >  1:118908/1‑148 (MQ=255)
                 acCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGt                                                                                                                                    >  2:1432682/1‑148 (MQ=255)
                 acCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGt                                                                                                                                    <  2:2659191/148‑1 (MQ=255)
                  ccAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTc                                                                                                                                   <  1:689309/148‑1 (MQ=255)
                  ccAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTc                                                                                                                                   >  1:2235998/1‑148 (MQ=255)
                  ccAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTc                                                                                                                                   <  1:1327194/148‑1 (MQ=255)
                      cTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCa                                                                                                                               <  2:3255497/148‑1 (MQ=255)
                        ccGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAAt                                                                                                                             >  1:1097849/1‑148 (MQ=255)
                        ccGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAAt                                                                                                                             >  1:2859819/1‑148 (MQ=255)
                           gCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGt                                                                                                                          <  2:2292378/148‑1 (MQ=255)
                            cacaATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGtt                                                                                                                         >  2:2604708/1‑148 (MQ=255)
                            cacaATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGtt                                                                                                                         >  1:563195/1‑148 (MQ=255)
                            cacaATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGtt                                                                                                                         <  1:3097015/148‑1 (MQ=255)
                             acaATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGttt                                                                                                                        <  2:2410898/148‑1 (MQ=255)
                               aaTGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTa                                                                                                                      <  1:1687222/148‑1 (MQ=255)
                                aTGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTaa                                                                                                                     >  2:3462/1‑148 (MQ=255)
                                  gCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATc                                                                                                                   <  2:3023333/148‑1 (MQ=255)
                                      tAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCt                                                                                                               >  1:3269903/1‑148 (MQ=255)
                                            aGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACAt                                                                                                         <  1:136707/148‑1 (MQ=255)
                                                    tGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGa                                                                                                 <  2:1441633/148‑1 (MQ=255)
                                                      gATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAAt                                                                                               >  2:1387809/1‑148 (MQ=255)
                                                       aTAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCTATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATg                                                                                              <  1:1450437/148‑1 (MQ=255)
                                                           aGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCttt                                                                                          >  2:685594/1‑148 (MQ=255)
                                                             gAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTa                                                                                        <  2:1135028/148‑1 (MQ=255)
                                                             gAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTa                                                                                        <  1:1865703/148‑1 (MQ=255)
                                                              aaCACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTAt                                                                                       >  1:1474931/1‑148 (MQ=255)
                                                               acacTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATg                                                                                      >  2:3734767/1‑148 (MQ=255)
                                                                  cTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCTATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCt                                                                                   >  1:1480738/1‑148 (MQ=255)
                                                                  cTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCt                                                                                   >  2:811276/1‑148 (MQ=255)
                                                                    tCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTAt                                                                                 >  2:793596/1‑148 (MQ=255)
                                                                      tGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATgg                                                                               <  2:2859819/148‑1 (MQ=255)
                                                                      tGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATgg                                                                               <  2:1097849/148‑1 (MQ=255)
                                                                         aCCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGgacg                                                                            <  2:127097/148‑1 (MQ=255)
                                                                          ccTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGgacga                                                                           >  2:3640952/1‑148 (MQ=255)
                                                                               aaGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCt                                                                      >  2:1335824/1‑148 (MQ=255)
                                                                               aaGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCt                                                                      <  2:1868451/148‑1 (MQ=255)
                                                                                 gCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCttt                                                                    >  2:2314708/1‑148 (MQ=255)
                                                                                 gCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCttt                                                                    >  2:392866/1‑148 (MQ=255)
                                                                                  cAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTc                                                                   >  2:1638525/1‑148 (MQ=255)
                                                                                    gTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCgg                                                                 <  1:2091616/148‑1 (MQ=255)
                                                                                     tGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGa                                                                >  2:565042/1‑148 (MQ=255)
                                                                                     tGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGa                                                                <  1:3412502/148‑1 (MQ=255)
                                                                                          tAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGcc                                                           >  1:3506176/1‑148 (MQ=255)
                                                                                          tAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGcc                                                           <  2:1703847/148‑1 (MQ=255)
                                                                                            aaCAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCtt                                                         <  2:1959942/148‑1 (MQ=255)
                                                                                            aaCAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCtt                                                         <  2:1845297/148‑1 (MQ=255)
                                                                                             aCAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCTATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTc                                                        <  2:2946325/148‑1 (MQ=255)
                                                                                             aCAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTc                                                        >  2:2099300/1‑148 (MQ=255)
                                                                                             aCAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTc                                                        >  2:2228669/1‑148 (MQ=255)
                                                                                             aCAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTc                                                        <  2:2593236/148‑1 (MQ=255)
                                                                                             aCAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTc                                                        <  2:675263/148‑1 (MQ=255)
                                                                                                ggCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGa                                                     <  2:814301/148‑1 (MQ=255)
                                                                                                 gCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCCATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCTGATGGCCTTCAGaa                                                    >  2:149410/1‑148 (MQ=255)
                                                                                                  ccTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAAc                                                   >  1:917834/1‑148 (MQ=255)
                                                                                                    tACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCTATCGTTGGTGGTGAAGTCGGCAATCGGTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCg                                                 >  1:3771242/1‑148 (MQ=255)
                                                                                                    tACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCg                                                 <  1:23076/148‑1 (MQ=255)
                                                                                                          cAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTa                                           >  2:2783551/1‑148 (MQ=255)
                                                                                                                 aaGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGa                                    <  2:118923/148‑1 (MQ=255)
                                                                                                                 aaGGAGTGAAACAGCGGTAACGACGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTGTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACAGGAGGTAAAAAGGa                                    >  2:220425/1‑148 (MQ=255)
                                                                                                                     aGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAg                                <  1:3734767/148‑1 (MQ=255)
                                                                                                                       tGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAgcg                              >  1:360422/1‑148 (MQ=255)
                                                                                                                         aaaCAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGcc                            <  1:708453/148‑1 (MQ=255)
                                                                                                                           aCAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCctc                          <  2:1532655/148‑1 (MQ=255)
                                                                                                                           aCAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCctc                          <  1:2660971/148‑1 (MQ=255)
                                                                                                                            cAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATCGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCctat                         >  2:3779374/1‑146 (MQ=255)
                                                                                                                             aGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCAAGAATGCTTTTATGGCTATGGACGACGCCTTTGGGATGGCCTTCATAACCGGAGGTAAAAAGGATCAGCGCCtctt                        <  1:2617303/148‑1 (MQ=255)
                                                                                                                               cGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCtcttct                      >  2:831483/1‑148 (MQ=255)
                                                                                                                               cGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCtcttct                      >  1:985649/1‑148 (MQ=255)
                                                                                                                                 gTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCttcttc                    <  1:603093/148‑1 (MQ=255)
                                                                                                                                  tAACGCCGACATAAAGCTATCGTTGGTGGTGAAGTCGGCACTCGTTTTAATCTGCTCGACATTCGCCAGAATGCTGTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTcc                   >  2:1375002/1‑148 (MQ=255)
                                                                                                                                    aCGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCgg                 >  2:1039475/1‑148 (MQ=255)
                                                                                                                                     cGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGgc                >  1:100331/1‑148 (MQ=255)
                                                                                                                                     cGCCGAAATAAAGCGATCGTGGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGgc                <  1:3515965/148‑1 (MQ=255)
                                                                                                                                      gCCGACATAAAGCTATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGgct               >  2:2105972/1‑148 (MQ=255)
                                                                                                                                       ccGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGgctg              <  2:3010252/148‑1 (MQ=255)
                                                                                                                                        cGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCAAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGgctgc             <  1:3064843/148‑1 (MQ=255)
                                                                                                                                          aCATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGctgctg           >  2:2397057/1‑148 (MQ=255)
                                                                                                                                           cATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGt          >  1:3627444/1‑148 (MQ=255)
                                                                                                                                             tAAAGCTATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGttt        >  1:2023261/1‑148 (MQ=255)
                                                                                                                                             tAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGttt        >  2:3223221/1‑148 (MQ=255)
                                                                                                                                              aaaGCTATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTa       >  1:1200071/1‑148 (MQ=255)
                                                                                                                                              aaaGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTa       >  1:2213411/1‑148 (MQ=255)
                                                                                                                                              aaaGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTa       >  1:819352/1‑148 (MQ=255)
                                                                                                                                              aaaGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTa       >  1:2213220/1‑148 (MQ=255)
                                                                                                                                               aaGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTaa      <  2:2445001/148‑1 (MQ=255)
                                                                                                                                                 gCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAAcc    >  1:2800776/1‑148 (MQ=255)
                                                                                                                                                 gCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAAcc    >  1:1364934/1‑148 (MQ=255)
                                                                                                                                                   gATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGAGGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCtg  <  1:1638561/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCAACTGCGGTCATACACCAACTCCGGCACAATGCGGTAATGCAGCGGGCTTGGATAAAGGAACACTTCTGCACCTGTAAGCAGTGGCGTAAACAGGCCTACCGTCAGCCCAAAGGAGTGAAACAGCGGTAACGCCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTG  >  CP009273/2968005‑2968299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑