Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,983,568 C→A 23.0% R51I (AGA→ATA)  yqeK ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,983,5680CA23.0% 1016.7 / 220.3 417R51I (AGA→ATA) yqeKuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (159/162);  new base A (54/41);  total (213/204)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

Only 100 of 435 total aligned reads displayed.

TCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGT  >  CP009273/2983421‑2983715
                                                                                                                                                   |                                                                                                                                                   
tCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATc                                                                                                                                                     <  1:23846/148‑1 (MQ=255)
 cAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCt                                                                                                                                                    <  1:1020901/148‑1 (MQ=255)
    tAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGa                                                                                                                                                 <  1:66657/148‑1 (MQ=255)
    tAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGa                                                                                                                                                 >  2:3484571/1‑148 (MQ=255)
     agagGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGaa                                                                                                                                                <  1:1628662/148‑1 (MQ=255)
      gagGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAAt                                                                                                                                               <  1:3046824/148‑1 (MQ=255)
       agGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAAtt                                                                                                                                              >  1:1341498/1‑148 (MQ=255)
         gAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTa                                                                                                                                            >  1:3699731/1‑148 (MQ=255)
         gAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTa                                                                                                                                            <  1:1228630/148‑1 (MQ=255)
         gAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTa                                                                                                                                            <  1:3307222/148‑1 (MQ=255)
          aaaGGACTAACGTTTCTTTAAAAGAATTGATTACATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTaa                                                                                                                                           <  2:2080602/148‑1 (MQ=255)
            aGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATg                                                                                                                                         <  2:696237/148‑1 (MQ=255)
            aGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATg                                                                                                                                         >  1:2117473/1‑148 (MQ=255)
            aGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATg                                                                                                                                         <  2:3633341/148‑1 (MQ=255)
             ggACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGt                                                                                                                                        <  1:972054/148‑1 (MQ=255)
                cTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCa                                                                                                                                     >  2:219987/1‑148 (MQ=255)
                    cGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCatgat                                                                                                                                 >  1:1186909/1‑148 (MQ=255)
                    cGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCatgat                                                                                                                                 >  2:2715487/1‑148 (MQ=255)
                          tttAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATgttgtt                                                                                                                           <  2:2798725/148‑1 (MQ=255)
                            tAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTg                                                                                                                         <  2:1118682/148‑1 (MQ=255)
                               aaGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGt                                                                                                                      >  2:256146/1‑148 (MQ=255)
                               aaGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGt                                                                                                                      >  2:2753828/1‑148 (MQ=255)
                                aGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTg                                                                                                                     >  1:3261314/1‑148 (MQ=255)
                                    ttGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGaa                                                                                                                 >  1:2747711/1‑148 (MQ=255)
                                     tGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAAc                                                                                                                >  1:1578652/1‑148 (MQ=255)
                                     tGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAAc                                                                                                                >  2:2170612/1‑148 (MQ=255)
                                      gATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACa                                                                                                               <  2:2624802/148‑1 (MQ=255)
                                           catcatCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATtctc                                                                                                          <  1:3184215/148‑1 (MQ=255)
                                              catcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGc                                                                                                       >  1:1828931/1‑148 (MQ=255)
                                               atcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCa                                                                                                      <  1:480235/148‑1 (MQ=255)
                                               atcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCa                                                                                                      >  1:2445586/1‑148 (MQ=255)
                                                tcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCAt                                                                                                     >  2:1668273/1‑148 (MQ=255)
                                                tcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCAt                                                                                                     <  1:2308896/148‑1 (MQ=255)
                                                   gTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTaa                                                                                                  <  2:73718/148‑1 (MQ=255)
                                                     taaactaaacTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAgg                                                                                                >  2:1985836/1‑148 (MQ=255)
                                                       aactaaactCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGaa                                                                                              <  1:2948674/148‑1 (MQ=255)
                                                         ctaaactCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAAtt                                                                                            <  1:3732784/148‑1 (MQ=255)
                                                           aaactCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAAtttt                                                                                          <  2:418354/148‑1 (MQ=255)
                                                               tcatcaTTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTa                                                                                      <  1:1918102/148‑1 (MQ=255)
                                                               tcatcaGTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTa                                                                                      <  2:2328790/148‑1 (MQ=255)
                                                                      tGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAAt                                                                               >  2:3760575/1‑148 (MQ=255)
                                                                      tGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAAt                                                                               >  1:3544837/1‑148 (MQ=255)
                                                                      tGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGAATTAAGGAATTTTTTTAACGGAAt                                                                               >  2:2520187/1‑148 (MQ=255)
                                                                        aCAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATcc                                                                             <  2:2453122/148‑1 (MQ=255)
                                                                        aCAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATcc                                                                             <  1:1854568/148‑1 (MQ=255)
                                                                        aCAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATcc                                                                             >  2:3612057/1‑148 (MQ=255)
                                                                         cAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCt                                                                            <  2:1520561/148‑1 (MQ=255)
                                                                         cAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCt                                                                            >  2:844317/1‑148 (MQ=255)
                                                                            aTCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTgg                                                                         >  2:1183606/1‑148 (MQ=255)
                                                                            aTCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTgg                                                                         >  1:870291/1‑148 (MQ=255)
                                                                             tCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTg                                                                          <  2:2126051/146‑1 (MQ=255)
                                                                             tCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGt                                                                        >  2:1032096/1‑148 (MQ=255)
                                                                             tCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGt                                                                        <  1:2843751/148‑1 (MQ=255)
                                                                               gTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGttt                                                                      <  1:456120/148‑1 (MQ=255)
                                                                                tGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTc                                                                     <  1:1389052/148‑1 (MQ=255)
                                                                                 gagaTATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCt                                                                    <  1:3120205/148‑1 (MQ=255)
                                                                                    atatAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCtttt                                                                 <  2:1341498/148‑1 (MQ=255)
                                                                                    atatAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCtttt                                                                 <  1:3796288/148‑1 (MQ=255)
                                                                                      ataACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTc                                                               >  2:327617/1‑148 (MQ=255)
                                                                                        aaCTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGa                                                             <  1:3437238/148‑1 (MQ=255)
                                                                                         aCTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAg                                                            <  2:526874/148‑1 (MQ=255)
                                                                                            gTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTcc                                                         >  1:2141203/1‑148 (MQ=255)
                                                                                             tttttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCg                                                        >  2:3447932/1‑148 (MQ=255)
                                                                                                ttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATTATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAAt                                                     <  2:1361662/148‑1 (MQ=255)
                                                                                                 tAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAAtt                                                    >  1:278068/1‑148 (MQ=255)
                                                                                                   aCTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTa                                                  <  2:947423/148‑1 (MQ=255)
                                                                                                      ttACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACaa                                               >  1:77445/1‑148 (MQ=255)
                                                                                                       tACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAat                                              >  2:182149/1‑148 (MQ=255)
                                                                                                       tACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAat                                              <  2:2267377/148‑1 (MQ=255)
                                                                                                          tctTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATc                                           <  1:3668272/148‑1 (MQ=255)
                                                                                                             ttACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATg                                        >  2:3470524/1‑148 (MQ=255)
                                                                                                                  ttGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCatat                                   >  1:3086517/1‑148 (MQ=255)
                                                                                                                   tGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAACGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCAtata                                  >  1:1484451/1‑148 (MQ=255)
                                                                                                                     aCTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGa                                >  2:1557968/1‑148 (MQ=255)
                                                                                                                       tttATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGAcc                              <  1:3484492/148‑1 (MQ=255)
                                                                                                                       tttATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGAcc                              >  1:2665934/1‑148 (MQ=255)
                                                                                                                        ttATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCa                             >  2:2837705/1‑148 (MQ=255)
                                                                                                                        ttATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCa                             <  2:177159/148‑1 (MQ=255)
                                                                                                                        ttATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCa                             <  1:519007/148‑1 (MQ=255)
                                                                                                                        ttATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCa                             <  2:3028684/148‑1 (MQ=255)
                                                                                                                         tATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCAt                            >  1:561403/1‑148 (MQ=255)
                                                                                                                           ttGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCAttt                          >  2:1466487/1‑148 (MQ=255)
                                                                                                                            tGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTc                         <  2:3246896/148‑1 (MQ=255)
                                                                                                                             gACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCa                        <  2:916808/148‑1 (MQ=255)
                                                                                                                             gACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCa                        <  1:249898/148‑1 (MQ=255)
                                                                                                                              aCAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCAt                       <  1:2160553/148‑1 (MQ=255)
                                                                                                                               cAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATg                      >  2:3345669/1‑148 (MQ=255)
                                                                                                                                  aaTTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAAt                   <  1:697872/148‑1 (MQ=255)
                                                                                                                                    ttAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCt                 <  1:1689293/148‑1 (MQ=255)
                                                                                                                                        cATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGa             >  2:3202319/1‑148 (MQ=255)
                                                                                                                                          ttCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGaaa           <  2:3074896/148‑1 (MQ=255)
                                                                                                                                           tCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGaaaa          <  1:1116576/148‑1 (MQ=255)
                                                                                                                                               atatCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCa      <  2:3678276/148‑1 (MQ=255)
                                                                                                                                               atatCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCa      <  1:219987/148‑1 (MQ=255)
                                                                                                                                               atatCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCa      >  1:2054996/1‑148 (MQ=255)
                                                                                                                                               atatCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCa      >  1:2704515/1‑148 (MQ=255)
                                                                                                                                               atatCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCa      <  2:3179374/148‑1 (MQ=255)
                                                                                                                                                   cTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGt  <  1:256146/148‑1 (MQ=255)
                                                                                                                                                   cTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGt  >  2:2364838/1‑148 (MQ=255)
                                                                                                                                                   cTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGt  >  2:2109547/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGT  >  CP009273/2983421‑2983715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑