Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,024,081 A→C 100% V70V (GTT→GTG)  ygfT ← putative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,024,0810AC97.8% 887.9 / 1.6 221V70V (GTT→GTG) ygfTputative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base C (152/64);  total (156/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 221 total aligned reads displayed.

TGCGCTGGTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCG  >  CP009273/3023936‑3024223
                                                                                                                                                 |                                                                                                                                              
tGCGCTGGTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCAc                                                                                                                                              <  1:263603/148‑1 (MQ=255)
      ggTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACa                                                                                                                                        >  2:2720898/1‑148 (MQ=255)
       gTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACaa                                                                                                                                       <  2:1636274/148‑1 (MQ=255)
             aaGGTCGCATTTCTGCGCAATCTTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACCTTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTa                                                                                                                                 <  1:2353560/148‑1 (MQ=255)
             aaGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTa                                                                                                                                 <  1:1532577/148‑1 (MQ=255)
               ggTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAAc                                                                                                                               >  1:2045366/1‑148 (MQ=255)
                    cATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAACGTCAGAGCATTCACCGGACAAGCCGTAACGCAAg                                                                                                                          >  2:2245938/1‑148 (MQ=255)
                    cATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAg                                                                                                                          >  2:1249567/1‑148 (MQ=255)
                       ttCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAgggg                                                                                                                       >  2:595271/1‑148 (MQ=255)
                       ttCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAgggg                                                                                                                       <  1:1391782/148‑1 (MQ=255)
                       ttCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAgggg                                                                                                                       >  2:3764011/1‑148 (MQ=255)
                         cTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCa                                                                                                                     <  2:1076286/148‑1 (MQ=255)
                           gcgcAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCAtt                                                                                                                   >  1:2627798/1‑148 (MQ=255)
                            cgcAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCAttg                                                                                                                  <  2:3140916/148‑1 (MQ=255)
                              cAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCAttgtt                                                                                                                >  1:288710/1‑148 (MQ=255)
                                 tCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCa                                                                                                             >  1:884762/1‑148 (MQ=255)
                                 tCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACA‑TTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAg                                                                                                            >  2:2191903/1‑148 (MQ=255)
                                  cGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAg                                                                                                            <  1:2401343/148‑1 (MQ=255)
                                    tATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTg                                                                                                          <  2:3004967/148‑1 (MQ=255)
                                    tATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTg                                                                                                          <  1:1184336/148‑1 (MQ=255)
                                       cGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATg                                                                                                       <  2:1287945/148‑1 (MQ=255)
                                         aCCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATTCATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGc                                                                                                     >  2:3531680/1‑148 (MQ=255)
                                         aCCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGc                                                                                                     >  1:2237322/1‑148 (MQ=255)
                                          ccATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCa                                                                                                    >  1:2953891/1‑148 (MQ=255)
                                              ctcAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGcc                                                                                                >  1:1507353/1‑148 (MQ=255)
                                                cAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCAc                                                                                              >  2:1809062/1‑148 (MQ=255)
                                                 aaCGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCAcc                                                                                             >  2:2197841/1‑148 (MQ=255)
                                                  acgacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCg                                                                                            >  1:1991265/1‑148 (MQ=255)
                                                  acgacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCg                                                                                            >  2:2585304/1‑148 (MQ=255)
                                                   cgacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCgg                                                                                           >  2:11530/1‑148 (MQ=255)
                                                   cgacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCgg                                                                                           >  2:2857760/1‑148 (MQ=255)
                                                   cgacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCgg                                                                                           >  1:716556/1‑148 (MQ=255)
                                                    gacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGa                                                                                          <  1:854192/148‑1 (MQ=255)
                                                     acgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGAt                                                                                         >  2:1334453/1‑148 (MQ=255)
                                                     acgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTACAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCAACGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGAt                                                                                         >  2:3450200/1‑148 (MQ=255)
                                                        ccAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCATCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCg                                                                                      <  2:3788217/148‑1 (MQ=255)
                                                            aGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGc                                                                                  >  1:3414637/1‑148 (MQ=255)
                                                            aGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGc                                                                                  <  1:745319/148‑1 (MQ=255)
                                                             ggggCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGcc                                                                                 <  1:1125007/148‑1 (MQ=255)
                                                             ggggCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGcc                                                                                 <  1:3742423/148‑1 (MQ=255)
                                                              gggCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGACAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCt                                                                                >  2:3474335/1‑148 (MQ=255)
                                                              gggCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCt                                                                                >  1:1904400/1‑148 (MQ=255)
                                                              gggCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCt                                                                                <  2:794618/148‑1 (MQ=255)
                                                              gggCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCt                                                                                >  2:535976/1‑148 (MQ=255)
                                                                 cAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGc                                                                             >  1:256764/1‑148 (MQ=255)
                                                                  aaGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGcc                                                                            <  1:99782/148‑1 (MQ=255)
                                                                     cGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCttt                                                                         >  1:1057286/1‑148 (MQ=255)
                                                                           cgcATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTAc                                                                   >  2:3179998/1‑148 (MQ=255)
                                                                             cATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACaa                                                                 >  2:40169/1‑148 (MQ=255)
                                                                              aTCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAAc                                                                >  1:2820541/1‑148 (MQ=255)
                                                                               tCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTGTCCCTACAACg                                                               >  2:2074671/1‑148 (MQ=255)
                                                                                    tACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGAt                                                          <  2:3412378/148‑1 (MQ=255)
                                                                                    tACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGAt                                                          >  1:123308/1‑148 (MQ=255)
                                                                                     aCAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATa                                                         >  2:1614969/1‑148 (MQ=255)
                                                                                     aCAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATa                                                         >  2:3509880/1‑148 (MQ=255)
                                                                                      cAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATAc                                                        >  2:3578578/1‑148 (MQ=255)
                                                                                       aaCCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATAcg                                                       >  2:2663823/1‑148 (MQ=255)
                                                                                           aaTACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGt                                                   >  2:2221502/1‑148 (MQ=255)
                                                                                            aTACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTc                                                  >  1:1818906/1‑148 (MQ=255)
                                                                                             tACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCg                                                 >  2:1767614/1‑148 (MQ=255)
                                                                                             tACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCg                                                 >  2:2444234/1‑148 (MQ=255)
                                                                                              aCATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGa                                                >  1:3777468/1‑148 (MQ=255)
                                                                                                aTTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGaaa                                              <  1:2720898/148‑1 (MQ=255)
                                                                                                 tttttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAg                                             >  2:1863667/1‑148 (MQ=255)
                                                                                                 tttttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAg                                             >  1:3633445/1‑148 (MQ=255)
                                                                                                  ttttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGt                                            >  2:3432731/1‑148 (MQ=255)
                                                                                                  ttttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGt                                            >  2:437068/1‑148 (MQ=255)
                                                                                                    ttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCa                                          <  1:580144/148‑1 (MQ=255)
                                                                                                    ttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCa                                          <  1:3531719/148‑1 (MQ=255)
                                                                                                         cGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACtgtg                                     >  2:177163/1‑148 (MQ=255)
                                                                                                            ccAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACt                                  <  2:2483826/148‑1 (MQ=255)
                                                                                                            ccAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACt                                  <  2:2773844/148‑1 (MQ=255)
                                                                                                             cAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGAGGATACGCGGTCGAAAGTCACTGTGACTc                                 <  2:2045366/148‑1 (MQ=255)
                                                                                                              aGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCa                                >  1:1340581/1‑148 (MQ=255)
                                                                                                                ttGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGc                              >  1:1963209/1‑148 (MQ=255)
                                                                                                                ttGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGc                              <  1:11524/148‑1 (MQ=255)
                                                                                                                ttGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGc                              >  1:2802792/1‑148 (MQ=255)
                                                                                                                ttGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGc                              >  1:3689256/1‑148 (MQ=255)
                                                                                                                   tACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGc                           >  2:2310557/1‑148 (MQ=255)
                                                                                                                    aCGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGcc                          <  1:1288271/148‑1 (MQ=255)
                                                                                                                     cGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCa                         >  2:3337862/1‑148 (MQ=255)
                                                                                                                       cTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGt                       >  1:3504983/1‑148 (MQ=255)
                                                                                                                       cTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGt                       >  1:1318816/1‑148 (MQ=255)
                                                                                                                         aTCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGttt                     >  1:169885/1‑148 (MQ=255)
                                                                                                                         aTCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGttt                     >  1:2468206/1‑148 (MQ=255)
                                                                                                                         aTCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGttt                     >  2:1732328/1‑148 (MQ=255)
                                                                                                                              aCTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTg                >  2:426563/1‑148 (MQ=255)
                                                                                                                               cTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGa               <  1:321197/148‑1 (MQ=255)
                                                                                                                               cTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGa               >  1:782015/1‑148 (MQ=255)
                                                                                                                                 ggAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGAtt             >  2:487173/1‑148 (MQ=255)
                                                                                                                                     aGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGACTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtgtgt         >  1:2552510/1‑148 (MQ=255)
                                                                                                                                      gTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtgtgtg        >  1:3575722/1‑148 (MQ=255)
                                                                                                                                      gTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtgtgtg        >  1:1681407/1‑148 (MQ=255)
                                                                                                                                       tCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGc       >  1:465543/1‑148 (MQ=255)
                                                                                                                                       tCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGc       >  1:2391679/1‑148 (MQ=255)
                                                                                                                                           agCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCAcc   >  1:3270476/1‑148 (MQ=255)
                                                                                                                                           agCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCAcc   >  1:3019088/1‑148 (MQ=255)
                                                                                                                                           agCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCAcc   >  1:1382484/1‑148 (MQ=255)
                                                                                                                                           agCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCAcc   >  2:2901053/1‑148 (MQ=255)
                                                                                                                                            gCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCg  >  2:1503144/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TGCGCTGGTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCG  >  CP009273/3023936‑3024223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑