Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,030,181 G→T 100% H429N (CAT→AAT)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,030,1810GT99.0% 616.0 / ‑1.7 192H429N (CAT→AAT) recJssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (132/58);  total (133/59)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 192 total aligned reads displayed.

ATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAA  >  CP009273/3030036‑3030325
                                                                                                                                                 |                                                                                                                                                
   tCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGc                                                                                                                                             <  1:1015477/148‑1 (MQ=255)
   tCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGc                                                                                                                                             <  1:1015502/148‑1 (MQ=255)
   tCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGc                                                                                                                                             <  1:1015473/148‑1 (MQ=255)
           gCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTc                                                                                                                                     <  1:1866031/148‑1 (MQ=255)
            cTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAGACTTCa                                                                                                                                    >  2:2428120/1‑148 (MQ=255)
                 cGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGcatc                                                                                                                               >  1:1464193/1‑148 (MQ=255)
                    aCCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATg                                                                                                                            >  2:3726199/1‑148 (MQ=255)
                      cGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGcc                                                                                                                          >  2:2741686/1‑148 (MQ=255)
                        tCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAg                                                                                                                        >  1:1350816/1‑148 (MQ=255)
                        tCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAg                                                                                                                        <  1:2391603/148‑1 (MQ=255)
                           gATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGt                                                                                                                     >  2:2877155/1‑148 (MQ=255)
                            aTACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAATGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTa                                                                                                                    >  2:623813/1‑148 (MQ=255)
                            aTACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTa                                                                                                                    >  1:2326224/1‑148 (MQ=255)
                              aCCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTaga                                                                                                                  >  2:3656848/1‑148 (MQ=255)
                              aCCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTaga                                                                                                                  <  2:3109469/148‑1 (MQ=255)
                               ccACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTagag                                                                                                                 >  2:85443/1‑148 (MQ=255)
                                  cTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtgt                                                                                                              >  1:1597222/1‑148 (MQ=255)
                                  cTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtgt                                                                                                              >  1:2579610/1‑148 (MQ=255)
                                  cTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtgt                                                                                                              >  2:1411783/1‑148 (MQ=255)
                                  cTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCATCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtgt                                                                                                              >  1:3496762/1‑148 (MQ=255)
                                    tCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtgtgt                                                                                                            >  2:3235340/1‑148 (MQ=255)
                                    tCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtgtgt                                                                                                            >  1:121238/1‑148 (MQ=255)
                                       ccTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTa                                                                                                         <  2:1130522/148‑1 (MQ=255)
                                       ccTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTa                                                                                                         <  1:876662/148‑1 (MQ=255)
                                       ccTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTa                                                                                                         <  2:3649409/148‑1 (MQ=255)
                                       ccTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTa                                                                                                         <  1:1428972/148‑1 (MQ=255)
                                        cTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCGTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTaa                                                                                                        <  2:225905/148‑1 (MQ=255)
                                        cTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGGTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTaa                                                                                                        >  1:3404388/1‑148 (MQ=255)
                                           gCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCg                                                                                                     <  2:3292570/148‑1 (MQ=255)
                                           gCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCg                                                                                                     <  1:311353/148‑1 (MQ=255)
                                              aTAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTc                                                                                                  >  2:3076059/1‑148 (MQ=255)
                                               tAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTcc                                                                                                 >  1:2874078/1‑148 (MQ=255)
                                                 gCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAg                                                                                               <  2:2148427/148‑1 (MQ=255)
                                                  cGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAgc                                                                                              >  1:3693445/1‑148 (MQ=255)
                                                    aaGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAgcgc                                                                                            >  2:2889464/1‑148 (MQ=255)
                                                       ggTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATc                                                                                         >  2:662436/1‑148 (MQ=255)
                                                              ccACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCata                                                                                  >  2:3539114/1‑148 (MQ=255)
                                                               cACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCatat                                                                                 >  2:392807/1‑148 (MQ=255)
                                                                aCTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATg                                                                                >  1:643808/1‑148 (MQ=255)
                                                                  tCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCa                                                                              >  1:2437202/1‑148 (MQ=255)
                                                                   cAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAg                                                                             >  1:3798694/1‑148 (MQ=255)
                                                                   cAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAg                                                                             >  2:1421306/1‑148 (MQ=255)
                                                                        aCCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCt                                                                        >  1:2629212/1‑148 (MQ=255)
                                                                         ccAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTg                                                                       >  1:2279096/1‑148 (MQ=255)
                                                                            gTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAAt                                                                    <  1:687419/148‑1 (MQ=255)
                                                                            gTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAAt                                                                    <  2:957499/148‑1 (MQ=255)
                                                                             ttCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATg                                                                   >  2:3740287/1‑148 (MQ=255)
                                                                             ttCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATg                                                                   >  2:478232/1‑148 (MQ=255)
                                                                             ttCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATg                                                                   >  1:1125013/1‑148 (MQ=255)
                                                                             ttCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATg                                                                   >  1:2852962/1‑148 (MQ=255)
                                                                             ttCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATg                                                                   >  1:2763552/1‑148 (MQ=255)
                                                                              tCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATgg                                                                  <  2:171588/148‑1 (MQ=255)
                                                                                 ccAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGc                                                               <  2:3511774/148‑1 (MQ=255)
                                                                                   aaaCCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGa                                                             >  1:3394767/1‑148 (MQ=255)
                                                                                     aCCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGAcc                                                           >  2:2666842/1‑148 (MQ=255)
                                                                                     aCCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGAcc                                                           >  2:1822877/1‑148 (MQ=255)
                                                                                      ccGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCg                                                          >  2:985361/1‑148 (MQ=255)
                                                                                         ttgttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGaa                                                       >  1:1872784/1‑148 (MQ=255)
                                                                                          tgttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAAc                                                      >  2:2308676/1‑148 (MQ=255)
                                                                                           gttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAAcc                                                     <  2:1481697/148‑1 (MQ=255)
                                                                                           gttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAAcc                                                     <  1:1566472/148‑1 (MQ=255)
                                                                                            ttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCt                                                    >  2:1951074/1‑148 (MQ=255)
                                                                                            ttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCt                                                    >  2:515430/1‑148 (MQ=255)
                                                                                               aaaGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTc                                                 >  2:130801/1‑148 (MQ=255)
                                                                                                 agagTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAg                                               >  2:3804737/1‑148 (MQ=255)
                                                                                                   agTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCg                                             >  2:592475/1‑148 (MQ=255)
                                                                                                   agTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCg                                             >  2:1198946/1‑148 (MQ=255)
                                                                                                     tttGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTa                                           >  1:2509177/1‑148 (MQ=255)
                                                                                                          aTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCTGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGACTGGAGCGACCGGAACCTTTCAGCGTACCGTc                                      <  1:3656848/148‑1 (MQ=255)
                                                                                                          aTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTc                                      >  2:1919832/1‑148 (MQ=255)
                                                                                                          aTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGAATCATGCCAGGTTAGTGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTc                                      <  1:470730/148‑1 (MQ=255)
                                                                                                           tttATCCTCTACCAGCGACAACCCCGCCGCCATCGCATTACCGCCAAACTACAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTCAATGGAGCGACCGGAACCTTTCAGCGTACCGTCa                                     <  1:405185/148‑1 (MQ=255)
                                                                                                               tCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctg                                 >  1:418359/1‑148 (MQ=255)
                                                                                                                ccTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgc                                >  1:2908143/1‑148 (MQ=255)
                                                                                                                          cGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAc                      >  2:1132985/1‑148 (MQ=255)
                                                                                                                          cGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAc                      >  2:978534/1‑148 (MQ=255)
                                                                                                                            aCAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgc                    >  2:855005/1‑148 (MQ=255)
                                                                                                                            aCAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgc                    >  2:582025/1‑148 (MQ=255)
                                                                                                                             cAAACCCGCCGCCATCGCCTTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCCTCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTGCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                   >  2:2468069/1‑148 (MQ=255)
                                                                                                                             cAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                   >  2:3009634/1‑148 (MQ=255)
                                                                                                                             cAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                   >  2:3012019/1‑148 (MQ=255)
                                                                                                                             cAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                   <  2:2874078/148‑1 (MQ=255)
                                                                                                                             cAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                   <  1:2070140/148‑1 (MQ=255)
                                                                                                                              aaaCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGa                  >  2:2546107/1‑148 (MQ=255)
                                                                                                                              aaaCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGa                  >  1:2090703/1‑148 (MQ=255)
                                                                                                                               aaCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGAt                 >  2:1236402/1‑148 (MQ=255)
                                                                                                                                aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATa                >  1:3811724/1‑148 (MQ=255)
                                                                                                                                aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATa                >  2:2313965/1‑148 (MQ=255)
                                                                                                                                aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATa                >  1:3811746/1‑148 (MQ=255)
                                                                                                                                 cccGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATaa               >  1:1366709/1‑148 (MQ=255)
                                                                                                                                 cccGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATaa               <  2:2589991/148‑1 (MQ=255)
                                                                                                                                     ccgccATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCgg           <  1:532772/148‑1 (MQ=255)
                                                                                                                                         cATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACgg       >  2:1970104/1‑148 (MQ=255)
                                                                                                                                           tCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTg     >  1:1256616/1‑148 (MQ=255)
                                                                                                                                           tCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTg     >  2:1463254/1‑148 (MQ=255)
                                                                                                                                            cGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGa    >  1:2351714/1‑148 (MQ=255)
                                                                                                                                             gCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaa   <  2:1320683/148‑1 (MQ=255)
                                                                                                                                             gCATTACCCCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaa   <  2:1462643/148‑1 (MQ=255)
                                                                                                                                              cATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaa  <  2:1597222/148‑1 (MQ=255)
                                                                                                                                              cATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaa  <  2:2852962/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAA  >  CP009273/3030036‑3030325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑