Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,110,751 C→A 100% R574L (CGT→CTT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,110,7510CA100.0% 555.8 / ‑6.7 186R574L (CGT→CTT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (89/95);  total (89/97)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

GGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGT  >  CP009273/3110606‑3110895
                                                                                                                                                 |                                                                                                                                                
ggCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCTTACACTGCCCATCTTTATTAACGCCCCCCATACAGCTGTAACCGTTCGGGCAAAGc                                                                                                                                                >  2:1483992/1‑148 (MQ=255)
ggCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGc                                                                                                                                                >  2:2421849/1‑148 (MQ=255)
ggCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGc                                                                                                                                                >  2:883817/1‑148 (MQ=255)
  cTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCaa                                                                                                                                              >  1:1180479/1‑148 (MQ=255)
    ttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCaaaa                                                                                                                                            <  2:3257460/148‑1 (MQ=255)
    ttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCaaaa                                                                                                                                            <  1:1556669/148‑1 (MQ=255)
         gTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtg                                                                                                                                       <  2:371061/148‑1 (MQ=255)
          tCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgt                                                                                                                                      <  1:1070776/148‑1 (MQ=255)
           ccGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgtt                                                                                                                                     >  2:2482800/1‑148 (MQ=255)
               cTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCCAAGCAAAATGCTGTTGTAg                                                                                                                                 >  1:3288423/1‑148 (MQ=255)
               cTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAg                                                                                                                                 >  2:1265937/1‑148 (MQ=255)
               cTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAg                                                                                                                                 <  1:2608589/148‑1 (MQ=255)
                ttCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgt                                                                                                                                >  2:3804352/1‑148 (MQ=255)
                 tCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgtg                                                                                                                               <  1:2245758/148‑1 (MQ=255)
                  ccATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgtgt                                                                                                                              <  2:708727/148‑1 (MQ=255)
                  ccATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgtgt                                                                                                                              <  1:3195381/148‑1 (MQ=255)
                    aTTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTgg                                                                                                                            <  2:603358/148‑1 (MQ=255)
                      ttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGt                                                                                                                          <  1:2865190/148‑1 (MQ=255)
                      ttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGt                                                                                                                          >  2:467848/1‑148 (MQ=255)
                      ttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGt                                                                                                                          >  1:2402458/1‑148 (MQ=255)
                      ttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGt                                                                                                                          >  2:3672387/1‑148 (MQ=255)
                           gtcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCg                                                                                                                     <  2:320704/148‑1 (MQ=255)
                            tcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGa                                                                                                                    >  1:2107384/1‑148 (MQ=255)
                             cgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGAt                                                                                                                   <  2:300603/148‑1 (MQ=255)
                               gACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATaa                                                                                                                 <  1:345358/148‑1 (MQ=255)
                                aCAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAAc                                                                                                                >  2:409069/1‑148 (MQ=255)
                                  aGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCa                                                                                                              <  2:8816/148‑1 (MQ=255)
                                  aGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAAAGCTGTTGTAGTGTGGGTTACCGATAACCa                                                                                                              >  1:1789441/1‑148 (MQ=255)
                                   gATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCAt                                                                                                             <  1:470566/148‑1 (MQ=255)
                                    aTAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATc                                                                                                            >  1:1512348/1‑148 (MQ=255)
                                       gcgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGt                                                                                                         <  2:3496934/148‑1 (MQ=255)
                                        cgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGtt                                                                                                        >  2:2992328/1‑148 (MQ=255)
                                        cgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGtt                                                                                                        >  1:157457/1‑148 (MQ=255)
                                        cgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGtt                                                                                                        <  2:2590307/148‑1 (MQ=255)
                                             caCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGccc                                                                                                   >  2:3270255/1‑148 (MQ=255)
                                               cGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTc                                                                                                 <  1:318147/148‑1 (MQ=255)
                                               cGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCAACAGTTTGCCCTc                                                                                                 >  1:1008977/1‑148 (MQ=255)
                                                     ccATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTTCCCATCTTTATTAACGCCCCCCGTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGAc                                                                                           >  1:2674163/1‑148 (MQ=255)
                                                      cATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACt                                                                                          <  2:2180288/148‑1 (MQ=255)
                                                           aGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGccc                                                                                     <  1:3653927/148‑1 (MQ=255)
                                                           aGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGccc                                                                                     >  2:2706406/1‑148 (MQ=255)
                                                           aGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGccc                                                                                     <  1:356476/148‑1 (MQ=255)
                                                            gTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGcccc                                                                                    >  2:824738/1‑148 (MQ=255)
                                                            gTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGcccc                                                                                    >  2:202771/1‑148 (MQ=255)
                                                             ttcttcATGTCATCCGGGTCGCTGTTGAGCGTACACAGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCAACGACTTGCCCCa                                                                                   >  1:1453686/1‑148 (MQ=255)
                                                              tcttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAg                                                                                  <  2:3482364/148‑1 (MQ=255)
                                                              tcttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAg                                                                                  <  2:1758240/148‑1 (MQ=255)
                                                                 tcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGa                                                                               >  2:65170/1‑148 (MQ=255)
                                                                 tcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACGTGCCCCAGCGa                                                                               >  2:1995398/1‑148 (MQ=255)
                                                                  cATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGaa                                                                              <  2:515715/148‑1 (MQ=255)
                                                                  cATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGaa                                                                              <  1:1996711/148‑1 (MQ=255)
                                                                  cATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGaa                                                                              <  1:1996761/148‑1 (MQ=255)
                                                                      tCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATaa                                                                          >  1:316982/1‑148 (MQ=255)
                                                                         tCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACg                                                                       <  2:3190574/148‑1 (MQ=255)
                                                                          ccGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAAAAAACgg                                                                      >  2:737592/1‑148 (MQ=255)
                                                                                  cTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTaa                                                              <  2:1610912/148‑1 (MQ=255)
                                                                                  cTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTaa                                                              >  1:2023307/1‑148 (MQ=255)
                                                                                  cTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTaa                                                              <  2:2039592/148‑1 (MQ=255)
                                                                                   tGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTaaa                                                             <  2:1789441/148‑1 (MQ=255)
                                                                                    gTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAg                                                            <  2:3159964/148‑1 (MQ=255)
                                                                                     ttGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAgg                                                           >  2:1308116/1‑148 (MQ=255)
                                                                                        aGCGTACACTGCCCATCTTTATTACCGCCCCCGTTCCAGCTGTACCCGTTCGGGAAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTgg                                                        <  2:2311090/148‑1 (MQ=255)
                                                                                        aGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGAAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTgg                                                        <  2:393445/148‑1 (MQ=255)
                                                                                        aGCGAACACTCCCCATCTTTATTACCCCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAATTGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATACACGGCAGGTTAAAGGTgg                                                        <  1:943686/148‑1 (MQ=255)
                                                                                         gCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTAACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAATGGTGGc                                                       >  2:1160584/1‑148 (MQ=255)
                                                                                         gCGTACACTGCCCATATTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGAAAACCAACAGTTTGCCCTCACCGACTTGCCCCAGCGAAATACACGGCAGGTTAAAGGTGGc                                                       <  2:3197731/148‑1 (MQ=255)
                                                                                          cGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCg                                                      >  1:631845/1‑148 (MQ=255)
                                                                                            tACTCTGCCCATCTTTATTAACGCCCCCGTTACAGCTGTAACCGTTCGGGCAAAGCAAAATGGTGTTGTTGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCAAGCGAAATAAACGGCAGGTTAAAGGTGGCGGt                                                    <  2:946369/148‑1 (MQ=255)
                                                                                            tACAGTGCCCATCTTTATAACCCCCCCCGTTCCAGCTGTACCCGTTCGGGCAAAGAAAAATGCTGTTGTAGTGTGGGTTACCTATAACCAACAGTTGGCCCACACCGACTTGCCCCCGCGAAATAAACGGCAGGTTAAAGGTGGCGGt                                                    <  1:2518851/148‑1 (MQ=255)
                                                                                             acacTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTa                                                   >  1:2543058/1‑148 (MQ=255)
                                                                                             acacTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTa                                                   >  1:1050947/1‑148 (MQ=255)
                                                                                             acaaTGCCCATCTGTATTAACGCCCCCGTTCCAGCTGTAACCGTGCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCATCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGtc                                                   >  2:963588/1‑147 (MQ=255)
                                                                                              cacTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTAt                                                  <  2:2174553/148‑1 (MQ=255)
                                                                                                      aTCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTa                                          >  2:1380462/1‑148 (MQ=255)
                                                                                                       tCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTaa                                         >  1:1327172/1‑148 (MQ=255)
                                                                                                          ttattaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGt                                      >  2:1741584/1‑148 (MQ=255)
                                                                                                           tattaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGtt                                     <  1:1265937/148‑1 (MQ=255)
                                                                                                            attaACGCCCCCGTTCCAGCTGTAACCGTTCGGGAAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGttt                                    <  2:2387104/148‑1 (MQ=255)
                                                                                                            atgaaCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAACTGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGttt                                    <  2:2642910/145‑1 (MQ=255)
                                                                                                             ttaCCGCCCCCGTTCCAGCTGTAACCGTCCGCGCAAAGAAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTGGCCCCAGCGAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGtttt                                   <  1:95596/148‑1 (MQ=255)
                                                                                                                 cGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCgg                               >  2:3786343/1‑148 (MQ=255)
                                                                                                                   cccccGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCACACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCt                             <  2:341474/148‑1 (MQ=255)
                                                                                                                    ccccGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTc                            >  1:2809857/1‑148 (MQ=255)
                                                                                                                        gTTCCAGCTGTAACGGTTCGGGCAAAGAAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCAAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTcaaca                        <  1:3474013/148‑1 (MQ=255)
                                                                                                                           ccAGCTGGAACCGTTCGGGCAAAGCATAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCTACTTGCCCAAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAaga                     <  2:154420/148‑1 (MQ=255)
                                                                                                                             aGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCTATAACCATCATTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAgaga                   <  1:3338388/148‑1 (MQ=255)
                                                                                                                              gCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGaa                  >  1:450625/1‑148 (MQ=255)
                                                                                                                              gCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGaa                  >  1:2371546/1‑148 (MQ=255)
                                                                                                                              gCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGaa                  >  1:1844797/1‑148 (MQ=255)
                                                                                                                                 gTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgc               >  2:956512/1‑148 (MQ=255)
                                                                                                                                   aaCCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCTATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgcgc             <  2:3161510/148‑1 (MQ=255)
                                                                                                                                   aaCCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCCCCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgcgc             <  2:379019/148‑1 (MQ=255)
                                                                                                                                     ccGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCtt           <  2:2594483/148‑1 (MQ=255)
                                                                                                                                     ccGTTCGGGAAAAGAAAATTGCTGTTGTTGTGTGGTTTACCGATAACCTTCTGTTTGCCCTCACCGACTTGCCCCTGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCtt           <  1:202771/148‑1 (MQ=255)
                                                                                                                                      cGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTc          <  2:770448/148‑1 (MQ=255)
                                                                                                                                       gTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTcc         <  2:1846790/148‑1 (MQ=255)
                                                                                                                                        ttCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCg        >  1:697307/1‑148 (MQ=255)
                                                                                                                                            ggCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAt    >  2:3045341/1‑148 (MQ=255)
                                                                                                                                             gcaaagcaaaATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATg   >  2:136970/1‑148 (MQ=255)
                                                                                                                                              cAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGt  <  1:3192023/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGT  >  CP009273/3110606‑3110895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑