Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,120,216 C→A 100% D389Y (GAT→TAT)  glcD ← glycolate oxidase subunit, FAD‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,120,2160CA100.0% 516.2 / ‑6.7 158D389Y (GAT→TAT) glcDglycolate oxidase subunit, FAD‑linked
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (83/74);  total (84/74)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 159 total aligned reads displayed.

TTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGG  >  CP009273/3120071‑3120357
                                                                                                                                                 |                                                                                                                                             
ttttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCAtatc                                                                                                                                             >  2:1879709/1‑148 (MQ=255)
    ctcGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGc                                                                                                                                         <  2:1003356/148‑1 (MQ=255)
    ctcGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGc                                                                                                                                         <  2:1002610/148‑1 (MQ=255)
    ctcGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGc                                                                                                                                         <  2:2017407/148‑1 (MQ=255)
      cGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCAt                                                                                                                                       >  2:3594592/1‑148 (MQ=255)
      cGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCAt                                                                                                                                       >  2:121558/1‑148 (MQ=255)
        cccGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGa                                                                                                                                     >  1:2437757/1‑148 (MQ=255)
         ccGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGaa                                                                                                                                    <  2:89364/148‑1 (MQ=255)
          cGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGaaa                                                                                                                                   >  2:2413131/1‑148 (MQ=255)
          cGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGaaa                                                                                                                                   >  1:2239206/1‑148 (MQ=255)
           gATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAACAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAg                                                                                                                                  >  2:3126601/1‑148 (MQ=255)
           gATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAg                                                                                                                                  >  2:2106251/1‑148 (MQ=255)
            aTGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGa                                                                                                                                 <  2:659134/148‑1 (MQ=255)
            aTGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGa                                                                                                                                 <  2:659082/148‑1 (MQ=255)
               ccATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGt                                                                                                                              <  2:2089405/148‑1 (MQ=255)
               ccATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGt                                                                                                                              >  1:162621/1‑148 (MQ=255)
               ccATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGt                                                                                                                              <  2:2918020/148‑1 (MQ=255)
                 aTGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTg                                                                                                                            <  2:1069186/148‑1 (MQ=255)
                  tGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTgg                                                                                                                           >  2:2840117/1‑148 (MQ=255)
                   gTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGc                                                                                                                          >  1:1096771/1‑148 (MQ=255)
                      cGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAac                                                                                                                       <  1:3697606/148‑1 (MQ=255)
                        ccACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAacac                                                                                                                     >  1:338116/1‑148 (MQ=255)
                            tGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTaa                                                                                                                 >  2:2193383/1‑148 (MQ=255)
                            tGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTaa                                                                                                                 <  1:3817296/148‑1 (MQ=255)
                            tGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTaa                                                                                                                 <  1:2037722/148‑1 (MQ=255)
                            tGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTaa                                                                                                                 >  2:127572/1‑148 (MQ=255)
                            tGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTaa                                                                                                                 >  2:1599771/1‑148 (MQ=255)
                            tGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTaa                                                                                                                 >  2:127578/1‑148 (MQ=255)
                            tGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTaa                                                                                                                 <  1:3817765/148‑1 (MQ=255)
                             gATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTaaa                                                                                                                >  2:1520774/1‑148 (MQ=255)
                                 ctgcCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCat                                                                                                            <  2:1847198/148‑1 (MQ=255)
                                       ccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgct                                                                                                      >  2:3017905/1‑148 (MQ=255)
                                           cTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGa                                                                                                  <  2:360948/148‑1 (MQ=255)
                                              cAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATaa                                                                                               >  2:1205236/1‑148 (MQ=255)
                                              cAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATaa                                                                                               <  1:1605398/148‑1 (MQ=255)
                                              cAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATaa                                                                                               <  2:2006105/148‑1 (MQ=255)
                                                 cGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACg                                                                                            <  1:1043547/148‑1 (MQ=255)
                                                 cGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACg                                                                                            >  2:3554720/1‑148 (MQ=255)
                                                   cAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACggg                                                                                          <  1:3206738/148‑1 (MQ=255)
                                                   cAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACggg                                                                                          >  2:3531766/1‑148 (MQ=255)
                                                   cAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACggg                                                                                          >  2:1420776/1‑148 (MQ=255)
                                                     gagTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCa                                                                                        >  1:1403138/1‑148 (MQ=255)
                                                      agTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCaa                                                                                       >  1:1018335/1‑148 (MQ=255)
                                                      agTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCaa                                                                                       <  2:1000516/148‑1 (MQ=255)
                                                      agTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCaa                                                                                       <  2:2889901/148‑1 (MQ=255)
                                                         tCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGc                                                                                    <  2:1096771/148‑1 (MQ=255)
                                                              gATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACTGGAAATGCCTTcc                                                                               >  2:3765002/1‑148 (MQ=255)
                                                              gATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTcc                                                                               <  1:731548/148‑1 (MQ=255)
                                                                 cTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGt                                                                            <  1:2238431/148‑1 (MQ=255)
                                                                 cTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGACATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGt                                                                            >  2:3416703/1‑148 (MQ=255)
                                                                  ttCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTa                                                                           >  1:2978965/1‑148 (MQ=255)
                                                                     ccGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGc                                                                        >  1:507479/1‑148 (MQ=255)
                                                                       gCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCca                                                                      <  1:3171071/148‑1 (MQ=255)
                                                                             ctctTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggcagg                                                                >  2:2338509/1‑148 (MQ=255)
                                                                             ctctTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggcagg                                                                <  1:1027725/148‑1 (MQ=255)
                                                                             ctctTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggcagg                                                                <  1:1334741/148‑1 (MQ=255)
                                                                              tctTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAggg                                                               <  1:1679657/148‑1 (MQ=255)
                                                                                         ggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCggg                                                    >  2:3400239/1‑148 (MQ=255)
                                                                                          gCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGa                                                   <  1:3017905/148‑1 (MQ=255)
                                                                                             aaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATgg                                                <  2:1403138/148‑1 (MQ=255)
                                                                                               ttCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTg                                              >  1:2063403/1‑148 (MQ=255)
                                                                                               ttCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTg                                              >  2:3637668/1‑148 (MQ=255)
                                                                                                tCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGc                                             >  1:2461211/1‑148 (MQ=255)
                                                                                                tCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGc                                             >  1:2459616/1‑148 (MQ=255)
                                                                                                tCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGc                                             <  1:3126601/148‑1 (MQ=255)
                                                                                                tCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGc                                             <  1:2106096/148‑1 (MQ=255)
                                                                                                   ccGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccat                                          <  1:3531766/148‑1 (MQ=255)
                                                                                                   ccGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccat                                          <  1:1879709/148‑1 (MQ=255)
                                                                                                     gggTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatcc                                        <  1:2370765/148‑1 (MQ=255)
                                                                                                           gTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCag                                  <  1:1563259/148‑1 (MQ=255)
                                                                                                            ttGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGTCGCGACGCGGGATGGTGCCATCCATGCagt                                 >  2:849733/1‑148 (MQ=255)
                                                                                                            ttGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagt                                 >  1:2281745/1‑148 (MQ=255)
                                                                                                            ttGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagt                                 <  2:310735/148‑1 (MQ=255)
                                                                                                              ggCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagtag                               <  2:979269/148‑1 (MQ=255)
                                                                                                                cATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAgtagta                             >  1:2827533/1‑148 (MQ=255)
                                                                                                                cATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAgtagta                             >  1:1756537/1‑148 (MQ=255)
                                                                                                                cATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAgtagta                             >  1:912945/1‑148 (MQ=255)
                                                                                                                  tCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAAt                           <  1:1366383/148‑1 (MQ=255)
                                                                                                                   cGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATc                          >  2:245575/1‑148 (MQ=255)
                                                                                                                     aaaaGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCg                        <  1:252142/148‑1 (MQ=255)
                                                                                                                     aaaaGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCg                        >  2:1006124/1‑148 (MQ=255)
                                                                                                                      aaaGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCgg                       >  1:2979968/1‑148 (MQ=255)
                                                                                                                      aaaGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCgg                       >  1:2980283/1‑148 (MQ=255)
                                                                                                                         ggATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGga                    >  2:621421/1‑148 (MQ=255)
                                                                                                                         ggATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGga                    >  2:2090277/1‑148 (MQ=255)
                                                                                                                          gATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGgag                   >  1:3090744/1‑148 (MQ=255)
                                                                                                                           aTTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGgaga                  >  1:2592613/1‑148 (MQ=255)
                                                                                                                           aTTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGgaga                  >  1:3718370/1‑148 (MQ=255)
                                                                                                                            ttAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAt                 <  2:2529149/148‑1 (MQ=255)
                                                                                                                            ttAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAt                 <  1:2208376/148‑1 (MQ=255)
                                                                                                                            ttAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAt                 >  1:2415975/1‑148 (MQ=255)
                                                                                                                            ttAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAt                 >  2:1559673/1‑148 (MQ=255)
                                                                                                                              aaCGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATAc               >  2:1019215/1‑148 (MQ=255)
                                                                                                                                 gggTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTc            >  2:1175443/1‑148 (MQ=255)
                                                                                                                                 gggTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTc            <  1:826201/148‑1 (MQ=255)
                                                                                                                                  ggTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTcc           <  1:2364861/148‑1 (MQ=255)
                                                                                                                                   gTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCt          >  2:3570021/1‑148 (MQ=255)
                                                                                                                                      cATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAcc       >  2:2042531/1‑148 (MQ=255)
                                                                                                                                       aTGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccg      >  1:3418483/1‑148 (MQ=255)
                                                                                                                                         gTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgcc    >  2:2642252/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGG  >  CP009273/3120071‑3120357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑