Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 250,909 A→T 100% L432Q (CTG→CAG)  pepD ← aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273250,9090AT97.7% 692.5 / 3.8 217L432Q (CTG→CAG) pepDaminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base T (116/95);  total (120/97)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

TTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTT  >  CP009273/250765‑251053
                                                                                                                                                |                                                                                                                                                
      agTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGt                                                                                                                                         <  2:3552342/148‑1 (MQ=255)
        ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTgg                                                                                                                                       >  2:861894/1‑148 (MQ=255)
        ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTgg                                                                                                                                       <  1:1225487/148‑1 (MQ=255)
         tcagtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGa                                                                                                                                      <  1:503460/148‑1 (MQ=255)
         tcagtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGa                                                                                                                                      >  1:2680858/1‑148 (MQ=255)
         tcagtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGa                                                                                                                                      >  2:3028344/1‑148 (MQ=255)
           agtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATa                                                                                                                                    <  2:3421839/148‑1 (MQ=255)
           agtcagCAGTGTCCAGTAATGACCTACGCGTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATa                                                                                                                                    <  2:2389857/148‑1 (MQ=255)
             tcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAAt                                                                                                                                  <  2:2668459/148‑1 (MQ=255)
              cagcagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATc                                                                                                                                 >  2:1546697/1‑148 (MQ=255)
               agcagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCt                                                                                                                                <  2:1202506/148‑1 (MQ=255)
                gcagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTg                                                                                                                               >  1:886013/1‑148 (MQ=255)
                 cagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTgg                                                                                                                              >  2:3257285/1‑148 (MQ=255)
                    tgtCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATg                                                                                                                           <  2:2086420/148‑1 (MQ=255)
                      tCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGtt                                                                                                                         >  2:1894831/1‑148 (MQ=255)
                         aGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCgg                                                                                                                      >  1:908649/1‑148 (MQ=255)
                            aaTGACCTACGCTTTCGATGTGAACTTGCTCATCCTGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGt                                                                                                                   <  2:1847251/148‑1 (MQ=255)
                            aaTGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGt                                                                                                                   <  1:1624270/148‑1 (MQ=255)
                            aaTGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGt                                                                                                                   <  1:3518687/148‑1 (MQ=255)
                            aaTGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGt                                                                                                                   <  1:1949434/148‑1 (MQ=255)
                             aTGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTc                                                                                                                  >  1:2787858/1‑148 (MQ=255)
                               gACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGTATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCtt                                                                                                                >  2:2059017/1‑148 (MQ=255)
                               gACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCtt                                                                                                                >  1:477560/1‑148 (MQ=255)
                                aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGTACAGACCTCATTCCTGGCCCGCGTGGATATTCTGGATGTTCGGCGTCttg                                                                                                               <  2:679115/148‑1 (MQ=255)
                                aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttg                                                                                                               >  2:1278476/1‑148 (MQ=255)
                                aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttg                                                                                                               <  2:518866/148‑1 (MQ=255)
                                aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttg                                                                                                               <  1:978584/148‑1 (MQ=255)
                                   tACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttgttg                                                                                                            >  2:3664226/1‑148 (MQ=255)
                                    aCGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGTATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                           >  2:2542912/1‑148 (MQ=255)
                                    aCGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                           >  2:1092859/1‑148 (MQ=255)
                                    aCGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                           >  2:3319902/1‑148 (MQ=255)
                                    aCGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                           <  2:3627400/148‑1 (MQ=255)
                                    aCGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                           >  1:1158268/1‑148 (MQ=255)
                                    aCGCTTTCGACGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                           >  1:1157258/1‑148 (MQ=255)
                                        tttCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAg                                                                                                       >  2:810337/1‑148 (MQ=255)
                                        tttCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAg                                                                                                       <  2:2236343/148‑1 (MQ=255)
                                        tttCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAg                                                                                                       >  2:3040413/1‑148 (MQ=255)
                                         ttCGATGTGAACGTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGTCCGATAGAAACCATGTCCATTTAAGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAgg                                                                                                      <  1:3652346/148‑1 (MQ=255)
                                           cGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGgcg                                                                                                    <  1:2589472/148‑1 (MQ=255)
                                             aTGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCt                                                                                                  >  1:3230318/1‑148 (MQ=255)
                                             aTGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCt                                                                                                  >  2:1814721/1‑148 (MQ=255)
                                             aTGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCt                                                                                                  >  1:1323613/1‑148 (MQ=255)
                                              tgtgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTg                                                                                                 <  1:811588/148‑1 (MQ=255)
                                               gtgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGa                                                                                                >  2:1955072/1‑148 (MQ=255)
                                               gtgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGa                                                                                                >  1:3581839/1‑148 (MQ=255)
                                               gtgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGa                                                                                                >  1:2642784/1‑148 (MQ=255)
                                                  aaCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAg                                                                                             >  1:1638012/1‑148 (MQ=255)
                                                    cTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGt                                                                                           <  2:2525642/148‑1 (MQ=255)
                                                      tGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGttt                                                                                         >  2:1385550/1‑148 (MQ=255)
                                                       gCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTc                                                                                        <  2:348313/148‑1 (MQ=255)
                                                        cTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCa                                                                                       <  1:2516480/148‑1 (MQ=255)
                                                          cATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACg                                                                                     >  2:2849515/1‑148 (MQ=255)
                                                          cATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACg                                                                                     >  1:820329/1‑148 (MQ=255)
                                                           aTCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGt                                                                                    <  1:839323/148‑1 (MQ=255)
                                                                gagaGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAg                                                                               <  1:309510/148‑1 (MQ=255)
                                                                gagaGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAg                                                                               <  1:1513240/148‑1 (MQ=255)
                                                                gagaGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAg                                                                               >  2:1597373/1‑148 (MQ=255)
                                                                 agagTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGa                                                                              <  1:2325853/148‑1 (MQ=255)
                                                                   agTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATg                                                                            >  2:829373/1‑148 (MQ=255)
                                                                      gtgGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCAt                                                                         <  2:3133954/148‑1 (MQ=255)
                                                                      gtgGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCAt                                                                         <  1:3656219/148‑1 (MQ=255)
                                                                        ggACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCa                                                                       <  1:1278476/148‑1 (MQ=255)
                                                                         gACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCAc                                                                      >  2:2993431/1‑148 (MQ=255)
                                                                              gTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGgag                                                                 <  2:3226111/148‑1 (MQ=255)
                                                                                gATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGaa                                                               <  1:2026750/148‑1 (MQ=255)
                                                                                   aGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTa                                                            <  2:3746981/148‑1 (MQ=255)
                                                                                        gCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTc                                                       >  1:3561587/1‑148 (MQ=255)
                                                                                             aTAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCt                                                  >  1:605016/1‑148 (MQ=255)
                                                                                               aGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgc                                                >  1:2237418/1‑148 (MQ=255)
                                                                                                 aaaCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgcca                                              >  2:1287948/1‑148 (MQ=255)
                                                                                                   aCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgccagc                                            <  1:1418672/148‑1 (MQ=255)
                                                                                                   aCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgccagc                                            <  1:183338/148‑1 (MQ=255)
                                                                                                        gtacATTTCCGGATACGTTTTTTTGAACAGACAACATTCGTGGCCGGAGTGGATAATGTGGATGTTGGGGGTCTTGTTGAACTGGCGGTTATAGGTTTAACGTACCTGTTGCATCACGGGTTATTTAGCGTCCGGCTGCCAGCCAGGa                                       <  1:2388231/145‑1 (MQ=255)
                                                                                                        gTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGa                                       <  2:886014/148‑1 (MQ=255)
                                                                                                          ccATTTCCGGATACGGTTTTTTGAACAGACCACCTGCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGata                                     <  2:2343942/148‑1 (MQ=255)
                                                                                                          ccATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGata                                     >  2:586052/1‑148 (MQ=255)
                                                                                                          ccATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGata                                     >  2:585999/1‑148 (MQ=255)
                                                                                                             tttCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATgc                                  >  2:3751034/1‑148 (MQ=255)
                                                                                                              ttCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATgcg                                 >  2:775132/1‑148 (MQ=255)
                                                                                                                 cGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCt                              <  2:447241/148‑1 (MQ=255)
                                                                                                                  ggATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCtt                             >  2:2671025/1‑148 (MQ=255)
                                                                                                                  ggATACGGTTTTTTGAACAGACCACATTCCGGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCtt                             <  1:1673255/148‑1 (MQ=255)
                                                                                                                      aCGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGc                         <  2:2742950/148‑1 (MQ=255)
                                                                                                                      aCGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGc                         >  2:3635163/1‑148 (MQ=255)
                                                                                                                            ttttGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGt                   <  2:1417162/148‑1 (MQ=255)
                                                                                                                            ttttGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGt                   >  1:423530/1‑148 (MQ=255)
                                                                                                                            ttttGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGt                   >  1:3304074/1‑148 (MQ=255)
                                                                                                                             tttGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGtt                  <  1:3812997/148‑1 (MQ=255)
                                                                                                                             tttGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTTTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGtt                  <  1:2059017/148‑1 (MQ=255)
                                                                                                                               tGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGtttt                <  1:1597373/148‑1 (MQ=255)
                                                                                                                               tGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGtttt                <  2:2691263/148‑1 (MQ=255)
                                                                                                                                 aaCAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTcg              >  1:1124374/1‑148 (MQ=255)
                                                                                                                                    aGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCgcgc           >  1:2383924/1‑148 (MQ=255)
                                                                                                                                    aGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCgcgc           >  2:635886/1‑148 (MQ=255)
                                                                                                                                    aGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCgcgc           >  1:3485165/1‑148 (MQ=255)
                                                                                                                                        cacaTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgccagc       >  2:1497844/1‑148 (MQ=255)
                                                                                                                                         actgtCCTGGCCCGCGTGGATAATCTGGAGGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgccagcc      <  2:172235/144‑1 (MQ=255)
                                                                                                                                         acaTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgccagcc      >  1:3809531/1‑148 (MQ=255)
                                                                                                                                            ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGt   >  1:173068/1‑148 (MQ=255)
                                                                                                                                             tCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGtt  >  1:1819207/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTT  >  CP009273/250765‑251053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑