Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,134,528 C→A 100% D40Y (GAT→TAT)  hybD ← maturation protease for hydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,134,5280CA97.0% 614.5 / 7.7 200D40Y (GAT→TAT) hybDmaturation protease for hydrogenase 2
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (103/91);  total (106/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

TTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCG  >  CP009273/3134383‑3134672
                                                                                                                                                 |                                                                                                                                                
ttgGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGa                                                                                                                                                <  2:2887014/148‑1 (MQ=255)
 tgGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATagag                                                                                                                                               <  1:1396012/148‑1 (MQ=255)
 tgGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATagag                                                                                                                                               <  1:1396343/148‑1 (MQ=255)
     aaaCAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtc                                                                                                                                           <  1:294543/148‑1 (MQ=255)
       acaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtctc                                                                                                                                         >  2:1043986/1‑148 (MQ=255)
         aacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCaa                                                                                                                                       <  2:3390592/148‑1 (MQ=255)
          acGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAAc                                                                                                                                      >  1:3294360/1‑148 (MQ=255)
             ccGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATa                                                                                                                                   <  2:3494141/148‑1 (MQ=255)
              cGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATaa                                                                                                                                  >  2:1541399/1‑148 (MQ=255)
               ggAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAAt                                                                                                                                 <  1:2033951/148‑1 (MQ=255)
                 aaCTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATcc                                                                                                                               >  2:1874812/1‑148 (MQ=255)
                      cttcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAg                                                                                                                          >  2:616935/1‑148 (MQ=255)
                        tcATCCCGCAGGATCATCATCGGTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGaa                                                                                                                        <  2:3650375/148‑1 (MQ=255)
                          aTCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATg                                                                                                                      <  1:3019277/148‑1 (MQ=255)
                          aTCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATg                                                                                                                      <  2:2624161/148‑1 (MQ=255)
                           tCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGt                                                                                                                     >  1:519512/1‑148 (MQ=255)
                           tCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGt                                                                                                                     <  2:3790974/148‑1 (MQ=255)
                            cccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTa                                                                                                                    <  2:70784/148‑1 (MQ=255)
                            cccGCAGGATCATCATCGGTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTa                                                                                                                    <  1:2068204/148‑1 (MQ=255)
                             ccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCACTAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTAt                                                                                                                   >  2:2895944/1‑148 (MQ=255)
                               gCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCg                                                                                                                 <  2:2431706/148‑1 (MQ=255)
                               gCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCg                                                                                                                 <  1:1307093/148‑1 (MQ=255)
                                cAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAAAGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGt                                                                                                                >  1:2923809/1‑148 (MQ=255)
                                  ggATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTg                                                                                                              >  1:386196/1‑148 (MQ=255)
                                       atcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTa                                                                                                         <  1:1743021/148‑1 (MQ=255)
                                       atcatcGTTCCCGGCGCGTTCTTTGTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTa                                                                                                         <  1:2675911/148‑1 (MQ=255)
                                          atcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACg                                                                                                      >  1:755955/1‑148 (MQ=255)
                                           tcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGc                                                                                                     <  1:1477364/148‑1 (MQ=255)
                                                  cGGCTCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACa                                                                                              <  2:3598098/148‑1 (MQ=255)
                                                  cGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACa                                                                                              <  2:1702396/148‑1 (MQ=255)
                                                  cGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACa                                                                                              <  2:3785382/148‑1 (MQ=255)
                                                  cGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACa                                                                                              <  2:3596681/148‑1 (MQ=255)
                                                  cGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACa                                                                                              <  2:3784472/148‑1 (MQ=255)
                                                     cgcgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATGTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATc                                                                                           <  1:2701620/148‑1 (MQ=255)
                                                          tCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGcac                                                                                      <  2:1362421/148‑1 (MQ=255)
                                                          tCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGcac                                                                                      >  2:2960592/1‑148 (MQ=255)
                                                              tttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCg                                                                                  >  2:3026253/1‑148 (MQ=255)
                                                                   agaCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGc                                                                             <  2:3273673/148‑1 (MQ=255)
                                                                    gaCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCt                                                                            <  1:2625544/148‑1 (MQ=255)
                                                                     aCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCtt                                                                           >  2:608862/1‑148 (MQ=255)
                                                                     aCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCtt                                                                           >  2:1053769/1‑148 (MQ=255)
                                                                     aCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCtt                                                                           >  2:1052922/1‑148 (MQ=255)
                                                                     aCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCtt                                                                           >  2:1967757/1‑148 (MQ=255)
                                                                     aCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCtt                                                                           >  1:1656636/1‑148 (MQ=255)
                                                                     aCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCtt                                                                           <  1:967880/148‑1 (MQ=255)
                                                                      cAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTc                                                                          >  1:3262256/1‑148 (MQ=255)
                                                                       aaTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATACTCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCa                                                                         <  2:3714374/148‑1 (MQ=255)
                                                                       aaTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCa                                                                         >  2:3804078/1‑148 (MQ=255)
                                                                       aaTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCa                                                                         >  2:355876/1‑148 (MQ=255)
                                                                       aaTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCa                                                                         <  2:700859/148‑1 (MQ=255)
                                                                         tGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATc                                                                       >  1:824170/1‑148 (MQ=255)
                                                                         tGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATc                                                                       >  1:1450012/1‑148 (MQ=255)
                                                                         tGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATc                                                                       >  2:3006910/1‑148 (MQ=255)
                                                                          ggCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCg                                                                      >  2:3069295/1‑148 (MQ=255)
                                                                            cATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGt                                                                    >  2:2097670/1‑148 (MQ=255)
                                                                               ccGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATNTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAg                                                                 >  1:1939405/1‑148 (MQ=255)
                                                                                  cAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaa                                                              >  1:3356640/1‑148 (MQ=255)
                                                                                   aataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaaa                                                             <  1:654415/148‑1 (MQ=255)
                                                                                    ataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaaaa                                                            >  2:33115/1‑148 (MQ=255)
                                                                                    ataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaaaa                                                            >  1:273131/1‑148 (MQ=255)
                                                                                     taatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAat                                                           >  2:485918/1‑148 (MQ=255)
                                                                                     taatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAat                                                           >  2:816257/1‑148 (MQ=255)
                                                                                      aatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAata                                                          >  2:3712410/1‑148 (MQ=255)
                                                                                      aatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAata                                                          >  1:2144824/1‑148 (MQ=255)
                                                                                       atCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                         >  1:3421151/1‑148 (MQ=255)
                                                                                       atCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                         >  1:3538314/1‑148 (MQ=255)
                                                                                        tCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATAtt                                                        <  1:2272571/148‑1 (MQ=255)
                                                                                        tCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATAtt                                                        >  2:3177710/1‑148 (MQ=255)
                                                                                         cAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                       <  1:3150376/148‑1 (MQ=255)
                                                                                         cAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                       <  1:2488900/148‑1 (MQ=255)
                                                                                         cAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                       <  2:755951/148‑1 (MQ=255)
                                                                                         cAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCAGTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                       <  2:1020412/148‑1 (MQ=255)
                                                                                          aaaTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGc                                                      <  2:2923809/148‑1 (MQ=255)
                                                                                           aaTGATCGCGATGTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGcc                                                     <  1:233027/148‑1 (MQ=255)
                                                                                             tGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGa                                                   >  1:1237657/1‑148 (MQ=255)
                                                                                                 cgcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAcccc                                               >  2:3120161/1‑148 (MQ=255)
                                                                                                 cgcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAcccc                                               <  2:2589535/148‑1 (MQ=255)
                                                                                                     aTTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAg                                           >  2:118076/1‑148 (MQ=255)
                                                                                                      tttGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGa                                          <  2:3775142/148‑1 (MQ=255)
                                                                                                       ttGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGAc                                         <  2:91321/148‑1 (MQ=255)
                                                                                                              gTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATa                                  <  1:1033928/148‑1 (MQ=255)
                                                                                                              gTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATa                                  >  1:2999009/1‑148 (MQ=255)
                                                                                                              gTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATa                                  >  1:3092467/1‑148 (MQ=255)
                                                                                                                cGCCAAGCAGCTCCAGTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACg                                <  2:2841155/148‑1 (MQ=255)
                                                                                                                     agcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa                           <  2:1757822/148‑1 (MQ=255)
                                                                                                                      gcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAc                          >  2:134938/1‑148 (MQ=255)
                                                                                                                      gcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAc                          >  2:2062785/1‑148 (MQ=255)
                                                                                                                          cTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGaa                      >  1:2849171/1‑148 (MQ=255)
                                                                                                                           tCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAc                     >  1:73428/1‑148 (MQ=255)
                                                                                                                              aTTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt                  <  1:1874812/148‑1 (MQ=255)
                                                                                                                               gtCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTc                 <  1:1730644/147‑1 (MQ=255)
                                                                                                                                   cgccgTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTg             >  1:3274424/1‑148 (MQ=255)
                                                                                                                                   cgccgTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTg             >  1:2771/1‑148 (MQ=255)
                                                                                                                                     ccgTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGaa           >  1:300436/1‑148 (MQ=255)
                                                                                                                                     ccgTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGaa           <  2:864016/148‑1 (MQ=255)
                                                                                                                                        tGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAAcc        <  1:3006921/148‑1 (MQ=255)
                                                                                                                                         gccgccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCa       <  2:2246657/148‑1 (MQ=255)
                                                                                                                                              cATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACTCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCg  <  1:3712470/148‑1 (MQ=255)
                                                                                                                                              cATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCg  >  2:1774279/1‑148 (MQ=255)
                                                                                                                                              cATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCg  >  1:2399191/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCG  >  CP009273/3134383‑3134672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑