Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,201,209 C→A 100% F160L (TTC→TTA)  ttdB → L‑tartrate dehydratase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,201,2090CA99.4% 543.6 / ‑4.2 168F160L (TTC→TTA) ttdBL‑tartrate dehydratase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (71/96);  total (72/96)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 168 total aligned reads displayed.

TGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTC  >  CP009273/3201066‑3201352
                                                                                                                                               |                                                                                                                                               
tGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGcc                                                                                                                                             <  2:862423/148‑1 (MQ=255)
  ccAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCg                                                                                                                                           <  2:257003/148‑1 (MQ=255)
  ccAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCg                                                                                                                                           <  1:2875283/148‑1 (MQ=255)
   cAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGc                                                                                                                                          >  1:559927/1‑148 (MQ=255)
      aaaTTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGa                                                                                                                                       >  1:2844772/1‑148 (MQ=255)
      aaaTTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGa                                                                                                                                       >  1:596234/1‑148 (MQ=255)
      aaaTTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGa                                                                                                                                       >  1:2844817/1‑148 (MQ=255)
      aaaTTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGa                                                                                                                                       >  1:2170221/1‑148 (MQ=255)
        aTTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGAtt                                                                                                                                     >  1:2279507/1‑148 (MQ=255)
         ttCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTg                                                                                                                                    >  2:2111910/1‑148 (MQ=255)
          tCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGt                                                                                                                                   <  1:651479/148‑1 (MQ=255)
          tCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGt                                                                                                                                   <  1:434730/148‑1 (MQ=255)
           cAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGtc                                                                                                                                  <  1:3652428/148‑1 (MQ=255)
           cAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGtc                                                                                                                                  <  1:3632194/148‑1 (MQ=255)
           cAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGtc                                                                                                                                  <  1:762490/148‑1 (MQ=255)
             aGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGtctc                                                                                                                                >  1:2636599/1‑148 (MQ=255)
               gcgcTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTa                                                                                                                              >  2:2897632/1‑148 (MQ=255)
                 gcTACCTGTGATGGTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCCCTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTAtt                                                                                                                            <  2:2759877/148‑1 (MQ=255)
                   tACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGa                                                                                                                          <  2:3107912/148‑1 (MQ=255)
                     cATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATa                                                                                                                        >  2:902790/1‑148 (MQ=255)
                     cATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATa                                                                                                                        >  2:2813930/1‑148 (MQ=255)
                       tgtgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCATAGAGTTAGGCCCGCTGATTGTCTCTATTGATAcc                                                                                                                      <  1:1193914/148‑1 (MQ=255)
                          gATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCAc                                                                                                                   <  2:3487937/148‑1 (MQ=255)
                             tttCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGc                                                                                                                >  1:2670288/1‑148 (MQ=255)
                             tttCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGc                                                                                                                >  2:2354785/1‑148 (MQ=255)
                             tttCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGc                                                                                                                <  2:3147267/148‑1 (MQ=255)
                                  cGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGcaacaa                                                                                                           <  2:214402/148‑1 (MQ=255)
                                  cGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGcaacaa                                                                                                           <  2:1390760/148‑1 (MQ=255)
                                    gCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAAcc                                                                                                         <  2:3521205/148‑1 (MQ=255)
                                       ggCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGa                                                                                                      >  1:2021104/1‑148 (MQ=255)
                                       ggCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGa                                                                                                      <  2:1988713/148‑1 (MQ=255)
                                           gcgcGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGc                                                                                                  >  2:2515303/1‑148 (MQ=255)
                                                gTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGaaa                                                                                             <  2:648597/148‑1 (MQ=255)
                                                gTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGaaa                                                                                             <  2:1323785/148‑1 (MQ=255)
                                                gTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACGGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGaaa                                                                                             <  2:862251/148‑1 (MQ=255)
                                                 tGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACCGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGaaaa                                                                                            >  1:3673361/1‑148 (MQ=255)
                                                 tGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGaaaa                                                                                            <  2:1946337/148‑1 (MQ=255)
                                                     ggcggcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACaaa                                                                                        <  2:835482/148‑1 (MQ=255)
                                                      gcggcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACaaaa                                                                                       >  2:1460610/1‑148 (MQ=255)
                                                      gcggcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACaaaa                                                                                       <  1:979471/148‑1 (MQ=255)
                                                        ggcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAg                                                                                     <  1:1382259/148‑1 (MQ=255)
                                                        ggcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAg                                                                                     <  1:3562888/148‑1 (MQ=255)
                                                         gcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGc                                                                                    <  2:186319/148‑1 (MQ=255)
                                                         gcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGc                                                                                    >  2:2679812/1‑148 (MQ=255)
                                                          cAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCt                                                                                   <  2:530680/148‑1 (MQ=255)
                                                           aaCCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGACCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTg                                                                                  <  2:2090984/148‑1 (MQ=255)
                                                             cccAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGtt                                                                                <  2:1193398/148‑1 (MQ=255)
                                                               cAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCg                                                                              <  1:2912619/148‑1 (MQ=255)
                                                               cAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCg                                                                              <  1:1286999/148‑1 (MQ=255)
                                                                  gTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCg                                                                           >  2:155721/1‑148 (MQ=255)
                                                                    ggAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                                                         <  1:1568217/148‑1 (MQ=255)
                                                                    ggAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                                                         <  2:3359898/148‑1 (MQ=255)
                                                                    ggAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                                                         >  2:3384883/1‑148 (MQ=255)
                                                                    ggAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                                                         <  1:521499/148‑1 (MQ=255)
                                                                     gAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAc                                                                        >  2:3153805/1‑148 (MQ=255)
                                                                     gAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAc                                                                        >  2:2225580/1‑148 (MQ=255)
                                                                     gAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAc                                                                        >  1:3546002/1‑148 (MQ=255)
                                                                       agagATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgc                                                                      <  2:2636557/148‑1 (MQ=255)
                                                                         agaTTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgccg                                                                    <  1:2992871/148‑1 (MQ=255)
                                                                             tGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGAt                                                                >  2:1087083/1‑148 (MQ=255)
                                                                               aagaagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATcc                                                              <  1:1029516/148‑1 (MQ=255)
                                                                               aagaagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATcc                                                              <  1:1029198/148‑1 (MQ=255)
                                                                                agaagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATccc                                                             <  1:490665/148‑1 (MQ=255)
                                                                                 gaagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCa                                                            <  2:3797221/148‑1 (MQ=255)
                                                                                  aagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCAt                                                           >  2:1433990/1‑148 (MQ=255)
                                                                                  aagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCAt                                                           <  2:2442327/148‑1 (MQ=255)
                                                                                  aagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCAt                                                           <  2:2442749/148‑1 (MQ=255)
                                                                                  aagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCAt                                                           <  2:618711/148‑1 (MQ=255)
                                                                                  aagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCAt                                                           >  2:51693/1‑148 (MQ=255)
                                                                                  aagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCAt                                                           >  2:3094819/1‑148 (MQ=255)
                                                                                     tGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGt                                                        >  2:3695418/1‑148 (MQ=255)
                                                                                        aCTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGa                                                     <  2:3584975/148‑1 (MQ=255)
                                                                                                gagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCt                                             >  2:971632/1‑148 (MQ=255)
                                                                                                gagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCt                                             >  1:3407438/1‑148 (MQ=255)
                                                                                                gagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGACACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCt                                             >  1:3097448/1‑148 (MQ=255)
                                                                                                 agCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTg                                            >  1:3810376/1‑148 (MQ=255)
                                                                                                   cTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg                                          <  2:2170221/148‑1 (MQ=255)
                                                                                                      ggAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGc                                       <  1:1280313/148‑1 (MQ=255)
                                                                                                      ggAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGc                                       <  2:3426472/148‑1 (MQ=255)
                                                                                                      ggAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGc                                       >  1:3691586/1‑148 (MQ=255)
                                                                                                        aaTGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                     >  2:2045685/1‑148 (MQ=255)
                                                                                                        aaTGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                     >  1:1419082/1‑148 (MQ=255)
                                                                                                        aaTGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                     >  1:2934803/1‑148 (MQ=255)
                                                                                                             cGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCa                                <  2:1697178/148‑1 (MQ=255)
                                                                                                                aGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTa                             >  2:2976559/1‑148 (MQ=255)
                                                                                                                 gTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTAc                            <  1:1445320/148‑1 (MQ=255)
                                                                                                                  tCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACa                           >  2:2937351/1‑148 (MQ=255)
                                                                                                                      tgtgGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATaaa                       <  1:2813930/148‑1 (MQ=255)
                                                                                                                      tgtgGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaa                       <  1:2384377/148‑1 (MQ=255)
                                                                                                                      tgtgGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaa                       <  1:2449804/148‑1 (MQ=255)
                                                                                                                       gtgGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaaa                      <  1:3809592/148‑1 (MQ=255)
                                                                                                                       gtgGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaaa                      >  1:2851616/1‑148 (MQ=255)
                                                                                                                          ggTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATaa                   <  2:3192003/148‑1 (MQ=255)
                                                                                                                            tCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAcc                 <  1:3221756/148‑1 (MQ=255)
                                                                                                                                ccGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTcc             <  1:1755381/148‑1 (MQ=255)
                                                                                                                                ccGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTcc             <  1:1755377/148‑1 (MQ=255)
                                                                                                                                        aaGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAgg     >  2:559743/1‑148 (MQ=255)
                                                                                                                                        aaGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAgg     >  1:2564276/1‑148 (MQ=255)
                                                                                                                                          gagTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCt   >  1:1766969/1‑148 (MQ=255)
                                                                                                                                           agTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTc  >  2:3683394/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTC  >  CP009273/3201066‑3201352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑