Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,290,970 A→T 100% R176R (CGT→CGA)  yraQ ← putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,290,9700AT97.4% 486.6 / 2.6 154R176R (CGT→CGA) yraQputative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base T (79/70);  total (83/71)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 154 total aligned reads displayed.

TAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCA  >  CP009273/3290826‑3291114
                                                                                                                                                |                                                                                                                                                
 aGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGaa                                                                                                                                              >  1:3283283/1‑148 (MQ=255)
 aGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGaa                                                                                                                                              <  1:869053/148‑1 (MQ=255)
  gcgcCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAAt                                                                                                                                             <  2:1945434/148‑1 (MQ=255)
   cgcCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATc                                                                                                                                            >  2:3503134/1‑148 (MQ=255)
   cgcCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATc                                                                                                                                            >  2:3124232/1‑148 (MQ=255)
     cccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATcgc                                                                                                                                          >  1:1174802/1‑148 (MQ=255)
     cccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATcgc                                                                                                                                          <  2:2437162/148‑1 (MQ=255)
       cTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATcgccg                                                                                                                                        >  2:240183/1‑148 (MQ=255)
        tGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATcgccgc                                                                                                                                       >  1:459019/1‑148 (MQ=255)
            ccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCaaaa                                                                                                                                   <  2:2103377/148‑1 (MQ=255)
                     aaaaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCg                                                                                                                          >  1:985982/1‑148 (MQ=255)
                     aaaaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCg                                                                                                                          <  2:278851/148‑1 (MQ=255)
                       aaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAg                                                                                                                        >  1:2000160/1‑148 (MQ=255)
                       aaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAg                                                                                                                        >  1:255070/1‑148 (MQ=255)
                       aaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACACCAGCCGAg                                                                                                                        >  1:2314982/1‑148 (MQ=255)
                        aaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAgg                                                                                                                       >  2:262106/1‑148 (MQ=255)
                          aCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCACTCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAATCCCCAGCCGAGGAc                                                                                                                     >  1:3019886/1‑148 (MQ=255)
                          aCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGAc                                                                                                                     >  1:880483/1‑148 (MQ=255)
                           cccGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACa                                                                                                                    <  2:131950/148‑1 (MQ=255)
                           cccGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACa                                                                                                                    >  1:2284759/1‑148 (MQ=255)
                            ccGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACaa                                                                                                                   >  1:2444793/1‑148 (MQ=255)
                                  tgtgCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCTCCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCa                                                                                                             >  1:1802447/1‑148 (MQ=255)
                                  tgtgCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCa                                                                                                             >  2:569648/1‑148 (MQ=255)
                                   gtgCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCAt                                                                                                            >  2:3498107/1‑148 (MQ=255)
                                   gtgCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCAt                                                                                                            >  1:187515/1‑148 (MQ=255)
                                     gCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATaa                                                                                                          <  2:3256675/148‑1 (MQ=255)
                                     gCTTCCGGGATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATaa                                                                                                          <  2:1625363/148‑1 (MQ=255)
                                        tCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAaca                                                                                                       <  1:1139813/148‑1 (MQ=255)
                                          cGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACAcc                                                                                                     <  1:1255386/148‑1 (MQ=255)
                                              aTGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCg                                                                                                 >  1:3209127/1‑148 (MQ=255)
                                              aTGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCg                                                                                                 >  1:1303901/1‑148 (MQ=255)
                                              aTGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCg                                                                                                 <  1:800632/148‑1 (MQ=255)
                                               tGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGt                                                                                                <  2:3389297/148‑1 (MQ=255)
                                               tGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGt                                                                                                <  2:157510/148‑1 (MQ=255)
                                               tGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGt                                                                                                <  2:1488372/148‑1 (MQ=255)
                                                gTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTc                                                                                               >  1:3046287/1‑148 (MQ=255)
                                                  cAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGc                                                                                             >  1:3118233/1‑148 (MQ=255)
                                                  cAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGc                                                                                             <  2:580720/148‑1 (MQ=255)
                                                    aTTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCg                                                                                           <  2:483063/148‑1 (MQ=255)
                                                    aTTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCg                                                                                           <  2:805076/148‑1 (MQ=255)
                                                      ttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCggg                                                                                         >  2:3670368/1‑148 (MQ=255)
                                                      ttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCggg                                                                                         <  1:1003796/148‑1 (MQ=255)
                                                      ttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCggg                                                                                         <  1:1003788/148‑1 (MQ=255)
                                                      ttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCggg                                                                                         >  2:1778525/1‑148 (MQ=255)
                                                        cGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGtt                                                                                       <  1:3695083/148‑1 (MQ=255)
                                                          aCCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTa                                                                                     <  2:1828856/148‑1 (MQ=255)
                                                           ccGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTaa                                                                                    >  2:1753545/1‑148 (MQ=255)
                                                                 ccTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCgg                                                                              <  2:3017678/148‑1 (MQ=255)
                                                                 ccTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCgg                                                                              <  2:3017681/148‑1 (MQ=255)
                                                                  cTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGa                                                                             <  1:3503134/148‑1 (MQ=255)
                                                                   tGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGAt                                                                            <  1:65477/148‑1 (MQ=255)
                                                                   tGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGAt                                                                            <  1:2279845/148‑1 (MQ=255)
                                                                    gCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGAtt                                                                           >  2:2062314/1‑148 (MQ=255)
                                                                    gCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGAtt                                                                           >  2:2062273/1‑148 (MQ=255)
                                                                          gCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCAt                                                                     <  2:2377591/148‑1 (MQ=255)
                                                                          gCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCAt                                                                     <  2:426942/148‑1 (MQ=255)
                                                                           cGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATc                                                                    <  2:256273/148‑1 (MQ=255)
                                                                           cGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATc                                                                    <  2:2019743/148‑1 (MQ=255)
                                                                              tgtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACACAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAg                                                                 >  1:617436/1‑148 (MQ=255)
                                                                              tgtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAg                                                                 >  1:2790017/1‑148 (MQ=255)
                                                                                  tCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATg                                                             <  2:3381356/148‑1 (MQ=255)
                                                                                   cACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGc                                                            <  1:3458143/148‑1 (MQ=255)
                                                                                       cACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAgc                                                        >  1:426749/1‑148 (MQ=255)
                                                                                         cccATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAgcgc                                                      <  2:1413148/148‑1 (MQ=255)
                                                                                          ccATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCa                                                     >  2:3814118/1‑148 (MQ=255)
                                                                                          ccATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCa                                                     <  2:2699433/148‑1 (MQ=255)
                                                                                            aTTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCAcc                                                   >  2:1666048/1‑148 (MQ=255)
                                                                                            aTTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCAcc                                                   >  2:1666047/1‑148 (MQ=255)
                                                                                              ttttGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGc                                                 >  2:879752/1‑148 (MQ=255)
                                                                                               tttGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGcc                                                <  2:187515/148‑1 (MQ=255)
                                                                                                ttGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGccc                                               <  2:3254463/148‑1 (MQ=255)
                                                                                                  gCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCAt                                             <  2:880483/148‑1 (MQ=255)
                                                                                                  gCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCAACAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCAt                                             <  2:3023714/148‑1 (MQ=255)
                                                                                                     ccAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGa                                          <  1:3101744/148‑1 (MQ=255)
                                                                                                     ccAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGa                                          <  1:3100834/148‑1 (MQ=255)
                                                                                                      cAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGac                                         >  1:377290/1‑148 (MQ=255)
                                                                                                      cAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGac                                         >  1:1403825/1‑148 (MQ=255)
                                                                                                        gCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGacac                                       <  2:294508/148‑1 (MQ=255)
                                                                                                         cGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACAcc                                      >  1:468376/1‑148 (MQ=255)
                                                                                                         cGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACAcc                                      >  1:45273/1‑148 (MQ=255)
                                                                                                           tCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTg                                    <  1:445731/148‑1 (MQ=255)
                                                                                                           tCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTg                                    <  1:2368369/148‑1 (MQ=255)
                                                                                                              cAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTg                                 <  2:3019906/148‑1 (MQ=255)
                                                                                                              cAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTg                                 >  1:2387082/1‑148 (MQ=255)
                                                                                                                aTCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGc                               <  1:2668537/148‑1 (MQ=255)
                                                                                                                    gCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACg                           >  2:2292244/1‑148 (MQ=255)
                                                                                                                    gCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACg                           >  1:2465389/1‑148 (MQ=255)
                                                                                                                     cAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGc                          >  1:992050/1‑148 (MQ=255)
                                                                                                                      aaCACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCCGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACCCCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCa                         <  1:17459/148‑1 (MQ=255)
                                                                                                                         acCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTc                      <  2:3580466/148‑1 (MQ=255)
                                                                                                                         acCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTc                      <  2:453516/148‑1 (MQ=255)
                                                                                                                         acCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTc                      <  1:3104046/148‑1 (MQ=255)
                                                                                                                         acCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTc                      <  2:3046287/148‑1 (MQ=255)
                                                                                                                           cATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTccc                    >  1:2910848/1‑148 (MQ=255)
                                                                                                                           cATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTccc                    >  1:2123622/1‑148 (MQ=255)
                                                                                                                                ccAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCcgccgc               >  2:1757120/1‑148 (MQ=255)
                                                                                                                                 cAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCcgcg              >  2:3595979/1‑148 (MQ=255)
                                                                                                                                        gCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAg       <  2:1238048/148‑1 (MQ=255)
                                                                                                                                             caGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGca  >  2:242524/1‑148 (MQ=255)
                                                                                                                                             caGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGca  >  1:1436570/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCA  >  CP009273/3290826‑3291114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑