Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,295,911 C→A 100% Q22K (CAG→AAG)  yhbV → U32 peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,295,9110CA99.4% 562.9 / ‑4.0 173Q22K (CAG→AAG) yhbVU32 peptidase family protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (101/71);  total (102/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 177 total aligned reads displayed.

GGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCACGC  >  CP009273/3295766‑3296052
                                                                                                                                                 |                                                                                                                                             
 gatggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATaagc                                                                                                                                            <  1:1270554/148‑1 (MQ=255)
  atggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATaagca                                                                                                                                           <  1:252955/148‑1 (MQ=255)
        aCGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGgccgc                                                                                                                                     >  2:2670590/1‑148 (MQ=255)
             cGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGccacca                                                                                                                                >  1:1600078/1‑148 (MQ=255)
                    aTGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAgcgc                                                                                                                         >  1:2931425/1‑148 (MQ=255)
                        ccGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGACGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGAc                                                                                                                     >  2:2728806/1‑148 (MQ=255)
                        ccGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGAc                                                                                                                     >  2:812869/1‑148 (MQ=255)
                         cGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACg                                                                                                                    >  2:2311306/1‑148 (MQ=255)
                            aGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGa                                                                                                                 <  2:1444499/148‑1 (MQ=255)
                             ggCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGAt                                                                                                                >  2:1594063/1‑148 (MQ=255)
                             ggCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGAt                                                                                                                >  1:717386/1‑148 (MQ=255)
                             ggCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGAt                                                                                                                <  2:382712/148‑1 (MQ=255)
                             ggCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATAGTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGAt                                                                                                                <  2:3602701/148‑1 (MQ=255)
                               cACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGAttt                                                                                                              <  2:871661/148‑1 (MQ=255)
                                 cGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTAt                                                                                                            <  1:2311769/148‑1 (MQ=255)
                                 cGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTAt                                                                                                            <  2:1266482/148‑1 (MQ=255)
                                 cGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTAt                                                                                                            >  2:3696445/1‑148 (MQ=255)
                                    aaaCCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCtt                                                                                                         >  1:3694413/1‑148 (MQ=255)
                                    aaaCCACTCGTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCtt                                                                                                         <  2:3573231/148‑1 (MQ=255)
                                      aCCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTgg                                                                                                       <  1:846747/148‑1 (MQ=255)
                                       ccACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGt                                                                                                      >  2:1669072/1‑148 (MQ=255)
                                       ccACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGt                                                                                                      >  2:3155194/1‑148 (MQ=255)
                                       ccACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGt                                                                                                      <  2:870565/148‑1 (MQ=255)
                                       ccACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGt                                                                                                      <  1:921823/148‑1 (MQ=255)
                                         aCTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGa                                                                                                    <  2:3818757/148‑1 (MQ=255)
                                          ctctTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGaa                                                                                                   >  1:1230593/1‑148 (MQ=255)
                                             ttGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCg                                                                                                <  1:2537845/148‑1 (MQ=255)
                                             ttGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCg                                                                                                <  2:2676636/148‑1 (MQ=255)
                                              tGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCgg                                                                                               >  2:1537497/1‑148 (MQ=255)
                                              tGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCgg                                                                                               >  1:1019399/1‑148 (MQ=255)
                                                gcgcATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTa                                                                                             <  1:2816650/148‑1 (MQ=255)
                                                     tatCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATgcag                                                                                        >  1:96793/1‑148 (MQ=255)
                                                      atCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATgcagc                                                                                       >  2:2612624/1‑148 (MQ=255)
                                                       tCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATgcagca                                                                                      >  1:3292589/1‑148 (MQ=255)
                                                         aCCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGACGCGGTATGCAGCAAg                                                                                    >  2:2527816/1‑148 (MQ=255)
                                                            gTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGt                                                                                 <  2:2385974/148‑1 (MQ=255)
                                                              aaaTGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCg                                                                               >  2:772657/1‑148 (MQ=255)
                                                              aaaTGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCg                                                                               >  1:319064/1‑148 (MQ=255)
                                                                aTGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCggg                                                                             >  1:3321560/1‑148 (MQ=255)
                                                                aTGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCggg                                                                             >  1:3035446/1‑148 (MQ=255)
                                                                 tGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGc                                                                            >  1:3027584/1‑148 (MQ=255)
                                                                    cAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAAc                                                                         >  2:2901083/1‑148 (MQ=255)
                                                                    cAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAAc                                                                         >  1:562073/1‑148 (MQ=255)
                                                                       tGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCaa                                                                      >  2:561818/1‑148 (MQ=255)
                                                                        gagaAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCaaa                                                                     <  1:3696445/148‑1 (MQ=255)
                                                                        gagaAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCaaa                                                                     <  2:1323667/148‑1 (MQ=255)
                                                                         agaAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAg                                                                    >  1:715339/1‑148 (MQ=255)
                                                                             aaGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTgg                                                                >  2:2137874/1‑148 (MQ=255)
                                                                              aGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGc                                                               >  1:1954917/1‑148 (MQ=255)
                                                                              aGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGc                                                               >  1:1699698/1‑148 (MQ=255)
                                                                              aGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGc                                                               >  1:1592877/1‑148 (MQ=255)
                                                                              aGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGc                                                               >  1:20891/1‑148 (MQ=255)
                                                                               gCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCg                                                              >  2:1697722/1‑148 (MQ=255)
                                                                                cAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTAGACGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAACTTGGCGa                                                             <  1:2860959/148‑1 (MQ=255)
                                                                                  aTGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGAct                                                           >  1:597523/1‑148 (MQ=255)
                                                                                   tGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGActg                                                          >  1:1791786/1‑148 (MQ=255)
                                                                                    gAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGActgg                                                         >  1:2459532/1‑148 (MQ=255)
                                                                                    gAAATATTCCTTAGGGCCAGTGCAGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGTGGActgg                                                         >  2:2161774/1‑148 (MQ=255)
                                                                                    gAAATAGTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGActgg                                                         <  1:3271289/148‑1 (MQ=255)
                                                                                        tatTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGActggctgg                                                     <  1:203348/148‑1 (MQ=255)
                                                                                          ttCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGgag                                                   >  2:2011089/1‑148 (MQ=255)
                                                                                          gtCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGgag                                                   <  2:3067039/147‑1 (MQ=255)
                                                                                          gtCCGTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGTCGCTGGAAGAATTTTATAAGCAGGCCGCCAGCAGCAGCGCCGACGTGATTTATCTTGGTGACTCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTTGCGACTGGCTGtag                                                   <  2:1162611/147‑1 (MQ=255)
                                                                                           tCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGgaga                                                  >  2:1154458/1‑148 (MQ=255)
                                                                                             cTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATg                                                <  2:319064/148‑1 (MQ=255)
                                                                                              ttAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAGGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATgg                                               >  1:1969371/1‑148 (MQ=255)
                                                                                              ttAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATgg                                               >  1:1966545/1‑148 (MQ=255)
                                                                                               tAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGc                                              >  2:1089080/1‑148 (MQ=255)
                                                                                               tAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGc                                              >  1:609572/1‑148 (MQ=255)
                                                                                                  ggCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCaaa                                           >  1:47293/1‑148 (MQ=255)
                                                                                                   gCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCaaaa                                          <  1:1004600/148‑1 (MQ=255)
                                                                                                   gCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCGTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCaaaa                                          >  2:1852108/1‑148 (MQ=255)
                                                                                                       gTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcgc                                      <  2:1990093/148‑1 (MQ=255)
                                                                                                        tGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcgct                                     <  1:1652566/148‑1 (MQ=255)
                                                                                                        tGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcgct                                     >  2:549452/1‑148 (MQ=255)
                                                                                                        tGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcgct                                     >  2:2675306/1‑148 (MQ=255)
                                                                                                        tGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcgct                                     <  1:418220/148‑1 (MQ=255)
                                                                                                               gTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCggg                              <  1:356743/148‑1 (MQ=255)
                                                                                                               gTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCggg                              <  1:3633453/148‑1 (MQ=255)
                                                                                                                     gCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGt                        <  2:96793/148‑1 (MQ=255)
                                                                                                                        aaaaGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAg                     >  1:1638531/1‑148 (MQ=255)
                                                                                                                         aaaGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGc                    >  2:501303/1‑148 (MQ=255)
                                                                                                                           agagACGCTGGAAGAATTTTCTAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAg                  <  2:1150117/148‑1 (MQ=255)
                                                                                                                           agagACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAg                  >  2:3331958/1‑148 (MQ=255)
                                                                                                                           agagACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAg                  >  2:937054/1‑148 (MQ=255)
                                                                                                                            gagaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGTAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGa                 >  1:2233704/1‑148 (MQ=255)
                                                                                                                            gagaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGa                 >  1:2380786/1‑148 (MQ=255)
                                                                                                                            gagaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGa                 <  1:3266373/148‑1 (MQ=255)
                                                                                                                             agaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGAt                <  2:959727/148‑1 (MQ=255)
                                                                                                                               aCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATtg              >  1:2039950/1‑148 (MQ=255)
                                                                                                                                   tGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGct          <  1:236596/148‑1 (MQ=255)
                                                                                                                                   tGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGct          <  1:217490/148‑1 (MQ=255)
                                                                                                                                   tGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGct          <  1:537031/148‑1 (MQ=255)
                                                                                                                                    ggAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGctc         <  2:716840/148‑1 (MQ=255)
                                                                                                                                    ggAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGctc         <  2:717386/148‑1 (MQ=255)
                                                                                                                                     gaagaaTTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGctct        <  2:3035422/148‑1 (MQ=255)
                                                                                                                                         aaTTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCAc    >  1:3807949/1‑148 (MQ=255)
                                                                                                                                           ttttATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCAcgc  >  2:3677691/1‑148 (MQ=255)
                                                                                                                                           ttttATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCAcgc  >  2:1714516/1‑148 (MQ=255)
                                                                                                                                           ttttATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCAcgc  >  2:2562072/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCACGC  >  CP009273/3295766‑3296052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑