Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,351,650 G→T 100% E1189D (GAG→GAT)  gltB → glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,351,6500GT99.0% 665.4 / ‑1.9 205E1189D (GAG→GAT) gltBglutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (92/110);  total (92/113)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

GTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATT  >  CP009273/3351506‑3351794
                                                                                                                                                |                                                                                                                                                
gTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACt                                                                                                                                               >  2:3399195/1‑148 (MQ=255)
 tttATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTg                                                                                                                                              <  2:3684478/148‑1 (MQ=255)
  ttATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGc                                                                                                                                             >  1:1410615/1‑148 (MQ=255)
  ttATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGc                                                                                                                                             >  1:3463404/1‑148 (MQ=255)
     tCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGa                                                                                                                                          >  2:2933703/1‑148 (MQ=255)
      cGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGaa                                                                                                                                         <  1:3678106/148‑1 (MQ=255)
         ccGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCg                                                                                                                                      <  1:636308/148‑1 (MQ=255)
          cGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGc                                                                                                                                     >  2:989336/1‑148 (MQ=255)
            tGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCAt                                                                                                                                   <  1:1104658/148‑1 (MQ=255)
             gAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATc                                                                                                                                  >  2:3454865/1‑148 (MQ=255)
             gAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATc                                                                                                                                  <  2:2113576/148‑1 (MQ=255)
             gAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATc                                                                                                                                  >  2:1309333/1‑148 (MQ=255)
              aaaCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATcc                                                                                                                                 <  2:776113/148‑1 (MQ=255)
               aaCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCa                                                                                                                                >  2:2315227/1‑148 (MQ=255)
               aaCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCa                                                                                                                                >  2:2310831/1‑148 (MQ=255)
               aaCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCa                                                                                                                                <  1:1242273/148‑1 (MQ=255)
                aCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAg                                                                                                                               >  1:118169/1‑148 (MQ=255)
                aCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAg                                                                                                                               >  2:1735876/1‑148 (MQ=255)
                 cccGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAgg                                                                                                                              <  1:1832811/148‑1 (MQ=255)
                 cccGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAgg                                                                                                                              <  1:3738866/148‑1 (MQ=255)
                 cccGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAgg                                                                                                                              <  1:1314036/148‑1 (MQ=255)
                           gttGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCGGTCGAAGCTGCTGGATACTGCCGAACCGCTTCCAGGTAAGGCActc                                                                                                                    <  1:3669515/146‑1 (MQ=255)
                            aTGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCActct                                                                                                                   >  2:588146/1‑148 (MQ=255)
                            aTGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCActct                                                                                                                   >  2:3287526/1‑148 (MQ=255)
                             tGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTa                                                                                                                  <  1:2679125/148‑1 (MQ=255)
                              ggCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTAc                                                                                                                 <  1:385388/148‑1 (MQ=255)
                               gCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACt                                                                                                                >  2:2418096/1‑148 (MQ=255)
                               gCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACt                                                                                                                >  2:3630491/1‑148 (MQ=255)
                                   aGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCAc                                                                                                            >  2:984552/1‑148 (MQ=255)
                                            tAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaacaa                                                                                                   <  1:43081/148‑1 (MQ=255)
                                                   tCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACccgccg                                                                                            <  1:2292356/148‑1 (MQ=255)
                                                     tggtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGtt                                                                                          <  2:1223287/148‑1 (MQ=255)
                                                       gtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTg                                                                                        <  1:3244647/148‑1 (MQ=255)
                                                        tggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGa                                                                                       >  1:3773048/1‑148 (MQ=255)
                                                          gATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATa                                                                                     <  2:1961548/148‑1 (MQ=255)
                                                           aTCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATaa                                                                                    <  2:2839880/148‑1 (MQ=255)
                                                            tCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAAc                                                                                   >  2:3247308/1‑148 (MQ=255)
                                                            tCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAAc                                                                                   >  2:1344827/1‑148 (MQ=255)
                                                             cTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACg                                                                                  >  2:2812684/1‑148 (MQ=255)
                                                              tGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACgg                                                                                 <  1:2860278/148‑1 (MQ=255)
                                                               gATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGc                                                                                <  2:680513/148‑1 (MQ=255)
                                                               gATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGc                                                                                <  1:2245390/148‑1 (MQ=255)
                                                                aTTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGcc                                                                               >  2:2835846/1‑148 (MQ=255)
                                                                   ggTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCctgc                                                                            <  2:2895247/148‑1 (MQ=255)
                                                                        cACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAc                                                                       >  1:2455512/1‑148 (MQ=255)
                                                                            gACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACgcgc                                                                   <  1:279246/148‑1 (MQ=255)
                                                                             aCCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCa                                                                  >  2:923627/1‑148 (MQ=255)
                                                                             aCCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGATGCTGGATACTGACGAACCGAATCCAGGTAAGGCACTATACAGCACCGAAAACAACCCGCCGTTTGATAAAGGACTGCTGAAAGCGCa                                                                  >  2:3406484/1‑148 (MQ=255)
                                                                               ctgctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGt                                                                <  2:1872680/148‑1 (MQ=255)
                                                                                tgctgAAAGAGCTGGACGGTTTCACCTCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGtt                                                               >  2:1869127/1‑148 (MQ=255)
                                                                                tgctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGtt                                                               >  2:1919153/1‑148 (MQ=255)
                                                                                 gctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtg                                                              >  2:267927/1‑148 (MQ=255)
                                                                                 gctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtg                                                              <  1:3014257/148‑1 (MQ=255)
                                                                                  ctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgc                                                             <  2:3270587/148‑1 (MQ=255)
                                                                                      aaGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCa                                                         >  2:2421181/1‑148 (MQ=255)
                                                                                        gagCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAAc                                                       <  2:1979087/148‑1 (MQ=255)
                                                                                        gagCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGATGCAAc                                                       <  1:2557012/148‑1 (MQ=255)
                                                                                         agCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACa                                                      >  2:3093872/1‑148 (MQ=255)
                                                                                               aCGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGaa                                                >  1:2996621/1‑148 (MQ=255)
                                                                                                cGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCTCCGTTTGATAACTGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGaaa                                               >  2:911070/1‑148 (MQ=255)
                                                                                                cGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGaaa                                               >  2:1078833/1‑148 (MQ=255)
                                                                                                cGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGaaa                                               <  2:412517/148‑1 (MQ=255)
                                                                                                 ggTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAAc                                              <  2:3720625/148‑1 (MQ=255)
                                                                                                  gTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAAcc                                             >  2:3533930/1‑148 (MQ=255)
                                                                                                       aCCTCCAAACAGCAGAAGCTGGCGCTGACGAAGCTGCTGGATACAGCCGAACAGCATCCACGTAAGGCACTCTACTGCACCGAAAACAACACGCCGTTTGATAACGGCCAGCTGAACGAGCAGTTGCTGCAACAGGCGAAACCGTTTg                                        >  2:3021345/1‑148 (MQ=255)
                                                                                                        ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGt                                       >  1:3159714/1‑148 (MQ=255)
                                                                                                           ccAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGa                                    >  2:3500712/1‑148 (MQ=255)
                                                                                                             aaaCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATg                                  <  1:3326186/148‑1 (MQ=255)
                                                                                                             aaaCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATg                                  <  2:2935705/148‑1 (MQ=255)
                                                                                                              aaCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGa                                 <  1:2769758/148‑1 (MQ=255)
                                                                                                              aaCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGa                                 <  2:627313/148‑1 (MQ=255)
                                                                                                               aCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAg                                <  1:3235311/148‑1 (MQ=255)
                                                                                                                     gAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCag                          >  1:2717907/1‑148 (MQ=255)
                                                                                                                      aaGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCaga                         >  1:194687/1‑148 (MQ=255)
                                                                                                                      aaGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCaga                         >  2:12922/1‑148 (MQ=255)
                                                                                                                           ggCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCaaa                    <  1:3132252/148‑1 (MQ=255)
                                                                                                                             cgcTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAAc                  <  1:566042/148‑1 (MQ=255)
                                                                                                                              gcTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAAcc                 <  2:1410615/148‑1 (MQ=255)
                                                                                                                                tGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCtt               <  2:2242305/148‑1 (MQ=255)
                                                                                                                                tGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCtt               <  2:3670540/148‑1 (MQ=255)
                                                                                                                                 gTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTc              <  1:2365223/148‑1 (MQ=255)
                                                                                                                                 gTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTc              <  2:1748811/148‑1 (MQ=255)
                                                                                                                                  tCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGt                                       >  2:1207157/1‑122 (MQ=255)
                                                                                                                                    gAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTgg           <  1:962787/148‑1 (MQ=255)
                                                                                                                                     aaGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTggg          <  2:1662581/148‑2 (MQ=255)
                                                                                                                                     aaGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGt          >  1:2459879/1‑148 (MQ=255)
                                                                                                                                       gctgctGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTc        >  2:3742304/1‑148 (MQ=255)
                                                                                                                                       gctgctGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTc        >  1:2810353/1‑148 (MQ=255)
                                                                                                                                         tgctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGa      <  2:2394306/148‑1 (MQ=255)
                                                                                                                                          gctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGat     <  2:3391364/148‑1 (MQ=255)
                                                                                                                                          gctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGat     <  1:1841355/148‑1 (MQ=255)
                                                                                                                                          gctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGat     <  1:3781931/148‑1 (MQ=255)
                                                                                                                                          gctgGATACTGCCGAACCGCAACCTGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCAGCTGAACGCGCAGTTGATGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCATAACCTTCTGGTTCGat     <  2:1228716/148‑1 (MQ=255)
                                                                                                                                           ctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGata    <  1:1545356/148‑1 (MQ=255)
                                                                                                                                           ctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGata    <  1:1128468/148‑1 (MQ=255)
                                                                                                                                           ctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGata    <  2:2567535/148‑1 (MQ=255)
                                                                                                                                           ctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGata    <  1:3037431/148‑1 (MQ=255)
                                                                                                                                            tgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGatat   <  1:3722392/148‑1 (MQ=255)
                                                                                                                                             ggATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATAtt  <  1:1663349/148‑1 (MQ=255)
                                                                                                                                             ggATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATAtt  <  1:539556/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATT  >  CP009273/3351506‑3351794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑