Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,445,410 C→A 100% R79L (CGT→CTT)  rplD ← 50S ribosomal subunit protein L4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,445,4100CA99.6% 801.3 / ‑4.4 242R79L (CGT→CTT) rplD50S ribosomal subunit protein L4
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (135/106);  total (135/107)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 242 total aligned reads displayed.

TTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAA  >  CP009273/3445265‑3445554
                                                                                                                                                 |                                                                                                                                                
ttCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGc                                                                                                                                                >  2:239079/1‑148 (MQ=255)
 tCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGcc                                                                                                                                               >  2:2092090/1‑148 (MQ=255)
    aCAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGa                                                                                                                                            >  1:3327886/1‑148 (MQ=255)
    aCAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGa                                                                                                                                            <  2:3369385/148‑1 (MQ=255)
     cAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGAt                                                                                                                                           >  1:2475074/1‑148 (MQ=255)
       gagaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCg                                                                                                                                         >  2:2972794/1‑148 (MQ=255)
       gagaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCg                                                                                                                                         <  2:3353544/148‑1 (MQ=255)
       gagaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCg                                                                                                                                         >  2:1404816/1‑148 (MQ=255)
        agaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCgg                                                                                                                                        >  1:224916/1‑148 (MQ=255)
        agaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCgg                                                                                                                                        >  2:1986515/1‑148 (MQ=255)
         gaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCggg                                                                                                                                       <  1:1543484/148‑1 (MQ=255)
          aaCTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGc                                                                                                                                      <  2:1793650/148‑1 (MQ=255)
          aaCTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGc                                                                                                                                      >  2:1080654/1‑148 (MQ=255)
          aaCTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGc                                                                                                                                      >  1:1621092/1‑148 (MQ=255)
           aCTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGct                                                                                                                                     >  2:3075578/1‑148 (MQ=255)
            cTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGctc                                                                                                                                    >  1:523817/1‑148 (MQ=255)
            cTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGctc                                                                                                                                    >  2:69405/1‑148 (MQ=255)
            cTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGctc                                                                                                                                    <  2:1639630/148‑1 (MQ=255)
               ctcGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTg                                                                                                                                 <  1:2124780/148‑1 (MQ=255)
               ctcGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTg                                                                                                                                 >  1:2212632/1‑148 (MQ=255)
                 cGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGAt                                                                                                                               >  1:1881107/1‑148 (MQ=255)
                   aCAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAg                                                                                                                             >  1:2441751/1‑148 (MQ=255)
                    cAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGa                                                                                                                            >  2:2956353/1‑148 (MQ=255)
                    cAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGa                                                                                                                            >  2:2955257/1‑148 (MQ=255)
                     aaCGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGaa                                                                                                                           >  2:3323672/1‑148 (MQ=255)
                      aCGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAAc                                                                                                                          >  1:144561/1‑148 (MQ=255)
                      aCGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAAc                                                                                                                          >  1:1214456/1‑148 (MQ=255)
                      aCGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAAc                                                                                                                          >  1:48181/1‑148 (MQ=255)
                       cGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAAcc                                                                                                                         <  1:813270/148‑1 (MQ=255)
                       cGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAAcc                                                                                                                         >  2:1509587/1‑148 (MQ=255)
                          tCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGa                                                                                                                      <  1:2384568/148‑1 (MQ=255)
                          tCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGa                                                                                                                      >  2:3672894/1‑148 (MQ=255)
                            aGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAc                                                                                                                    >  1:3772961/1‑148 (MQ=255)
                             gACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcg                                                                                                                   >  2:3520761/1‑148 (MQ=255)
                             gACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcg                                                                                                                   >  2:853401/1‑148 (MQ=255)
                             gACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcg                                                                                                                   <  2:1617346/148‑1 (MQ=255)
                               cGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcgcg                                                                                                                 >  1:2073613/1‑148 (MQ=255)
                                gATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCTCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCTGAAGAGCAGCAAAGGTCACTCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACgcgc                                                                                                                <  2:3095676/148‑1 (MQ=255)
                                  tCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCAc                                                                                                              >  2:2248073/1‑148 (MQ=255)
                                          tACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTg                                                                                                      <  2:967514/148‑1 (MQ=255)
                                           aCCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGc                                                                                                     <  2:1433319/148‑1 (MQ=255)
                                           aCCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGc                                                                                                     >  1:1777772/1‑148 (MQ=255)
                                            ccAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc                                                                                                    <  1:3473323/148‑1 (MQ=255)
                                            ccAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc                                                                                                    <  1:1954227/148‑1 (MQ=255)
                                             cAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCt                                                                                                   <  2:2073613/148‑1 (MQ=255)
                                               gTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCttt                                                                                                 <  1:1355279/148‑1 (MQ=255)
                                                ttCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTc                                                                                                >  1:2101892/1‑148 (MQ=255)
                                                ttCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTc                                                                                                <  2:1059546/148‑1 (MQ=255)
                                                        ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCa                                                                                        >  2:396063/1‑148 (MQ=255)
                                                           tGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACgg                                                                                     >  1:244683/1‑148 (MQ=255)
                                                               tttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                                                                 <  2:3489173/148‑1 (MQ=255)
                                                                 tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttttt                                                                               <  1:1428870/148‑1 (MQ=255)
                                                                    gcgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAcc                                                                            >  1:3131260/1‑148 (MQ=255)
                                                                     cgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCg                                                                           >  1:2507913/1‑148 (MQ=255)
                                                                     cgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTATACCg                                                                           >  2:2000344/1‑148 (MQ=255)
                                                                         ccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAc                                                                       >  2:1869677/1‑148 (MQ=255)
                                                                            cgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                                                                    >  2:2199026/1‑148 (MQ=255)
                                                                             ggTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGt                                                                   <  1:3163456/148‑1 (MQ=255)
                                                                               tACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTa                                                                 <  2:262877/148‑1 (MQ=255)
                                                                               tACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTa                                                                 <  2:2212632/148‑1 (MQ=255)
                                                                                aCATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc                                                                >  1:1056514/1‑148 (MQ=255)
                                                                                  aTCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACtt                                                              >  1:975624/1‑148 (MQ=255)
                                                                                  aTCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACtt                                                              >  1:1645345/1‑148 (MQ=255)
                                                                                   tcttctTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc                                                             >  2:2547695/1‑148 (MQ=255)
                                                                                     ttcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAg                                                           >  2:1293152/1‑148 (MQ=255)
                                                                                     ttcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAg                                                           >  2:3198338/1‑148 (MQ=255)
                                                                                      tcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGc                                                          <  1:185677/148‑1 (MQ=255)
                                                                                      tcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGc                                                          >  1:3811887/1‑148 (MQ=255)
                                                                                         tGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACg                                                       <  1:1739668/148‑1 (MQ=255)
                                                                                             aaCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtc                                                   <  2:3130034/148‑1 (MQ=255)
                                                                                              aCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtct                                                  >  1:2790799/1‑148 (MQ=255)
                                                                                              aCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtct                                                  >  1:2790682/1‑148 (MQ=255)
                                                                                               cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtctt                                                 >  1:2520233/1‑148 (MQ=255)
                                                                                               cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtctt                                                 >  1:378478/1‑148 (MQ=255)
                                                                                                tttttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttc                                                <  2:2534711/148‑1 (MQ=255)
                                                                                                 ttttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttct                                               >  1:1104614/1‑148 (MQ=255)
                                                                                                  tttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTg                                              <  2:1214456/148‑1 (MQ=255)
                                                                                                     gACTGTGGTCCAGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGc                                           <  2:2837953/148‑1 (MQ=255)
                                                                                                        tgtgGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACg                                        >  1:2854568/1‑148 (MQ=255)
                                                                                                        tgtgGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACg                                        <  1:766119/148‑1 (MQ=255)
                                                                                                         gtgGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGa                                       >  2:3493114/1‑148 (MQ=255)
                                                                                                              ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGCTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTAcc                                  <  1:2350582/148‑1 (MQ=255)
                                                                                                              ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTAcc                                  >  1:2896439/1‑148 (MQ=255)
                                                                                                              ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTAcc                                  <  2:1902562/148‑1 (MQ=255)
                                                                                                              ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTAcc                                  >  2:2020202/1‑148 (MQ=255)
                                                                                                                tGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCt                                <  2:523817/148‑1 (MQ=255)
                                                                                                                tGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCt                                <  2:21446/148‑1 (MQ=255)
                                                                                                                tGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCt                                >  1:3122888/1‑148 (MQ=255)
                                                                                                                 gCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTg                               <  1:2972794/148‑1 (MQ=255)
                                                                                                                       gAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGc                         <  1:3691836/148‑1 (MQ=255)
                                                                                                                           agcaAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCa                     <  1:1306841/148‑1 (MQ=255)
                                                                                                                             caAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGc                   >  2:218364/1‑148 (MQ=255)
                                                                                                                               aaGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAACAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTg                 >  2:568460/1‑148 (MQ=255)
                                                                                                                                       gCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGca         <  1:607889/148‑1 (MQ=255)
                                                                                                                                       gCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGca         <  1:2583126/148‑1 (MQ=255)
                                                                                                                                            caGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTATGGCGCCACGGTTTTTTAACGGAACCACTTACTTAAGCAAGAGTCTTCAGATCAAGAGTAACCAGACGAGCACCAGCTGCATAAGcaacaac    >  1:18883/1‑148 (MQ=255)
                                                                                                                                            caGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCGTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaacaac    >  1:19917/1‑148 (MQ=255)
                                                                                                                                             aGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaacaaca   <  2:3772961/148‑1 (MQ=255)
                                                                                                                                             aGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaacaaca   <  2:2461719/148‑1 (MQ=255)
                                                                                                                                              gAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaacaacaa  >  1:2074562/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAA  >  CP009273/3445265‑3445554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑