Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 279,334 C→A 100% I141I (ATC→ATA)  yagF → CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273279,3340CA98.9% 542.7 / ‑1.8 175I141I (ATC→ATA) yagFCP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (90/82);  total (91/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 175 total aligned reads displayed.

CATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGAC  >  CP009273/279190‑279477
                                                                                                                                                |                                                                                                                                               
cATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgtg                                                                                                                                              >  2:2376886/1‑148 (MQ=255)
     agatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcg                                                                                                                                         <  2:2519214/148‑1 (MQ=255)
      gatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTCTGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgc                                                                                                                                        >  2:3189078/1‑148 (MQ=255)
      gatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgc                                                                                                                                        <  1:1224510/148‑1 (MQ=255)
        tgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgccg                                                                                                                                      >  2:797475/1‑148 (MQ=255)
            ggcggcggcGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTg                                                                                                                                  <  1:2469591/148‑1 (MQ=255)
             gcggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGa                                                                                                                                 >  2:2621633/1‑148 (MQ=255)
              cggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGAt                                                                                                                                <  1:1080641/148‑1 (MQ=255)
              cggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGAt                                                                                                                                <  1:1080235/148‑1 (MQ=255)
                      aaGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTccc                                                                                                                        <  1:248713/148‑1 (MQ=255)
                       aGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCt                                                                                                                       <  1:2527213/148‑1 (MQ=255)
                       aGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCt                                                                                                                       >  1:3437063/1‑148 (MQ=255)
                        ggAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTg                                                                                                                      >  2:1417136/1‑148 (MQ=255)
                        ggAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTg                                                                                                                      <  2:3805213/148‑1 (MQ=255)
                         gagaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGc                                                                                                                     >  1:1160370/1‑148 (MQ=255)
                         gagaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGc                                                                                                                     >  1:3292723/1‑148 (MQ=255)
                         gagaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGc                                                                                                                     >  1:3108958/1‑148 (MQ=255)
                           gaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCg                                                                                                                   >  1:2296704/1‑148 (MQ=255)
                            aTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGa                                                                                                                  >  1:578611/1‑148 (MQ=255)
                            aTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGa                                                                                                                  >  1:1770090/1‑148 (MQ=255)
                             tCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAc                                                                                                                 <  1:2786552/148‑1 (MQ=255)
                               aCCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAcgc                                                                                                               >  2:2260787/1‑148 (MQ=255)
                               aCCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAcgc                                                                                                               >  2:1611281/1‑148 (MQ=255)
                                cccGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAcgcg                                                                                                              >  2:791697/1‑148 (MQ=255)
                                cccGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAcgcg                                                                                                              >  2:2270591/1‑148 (MQ=255)
                                   gCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACgcggcg                                                                                                           <  2:1195218/148‑1 (MQ=255)
                                   gCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACgcggcg                                                                                                           <  2:2447552/148‑1 (MQ=255)
                                    cAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGTCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGcggcgg                                                                                                          >  1:2822447/1‑148 (MQ=255)
                                    cAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGcggcgg                                                                                                          >  1:2693313/1‑148 (MQ=255)
                                       tGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCggcgggcg                                                                                                       <  2:2115888/148‑1 (MQ=255)
                                        ggCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGgcgggcgg                                                                                                      <  2:2931404/148‑1 (MQ=255)
                                             gATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATc                                                                                                 <  2:2023613/148‑1 (MQ=255)
                                              aTCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCg                                                                                                >  2:3535751/1‑148 (MQ=255)
                                                cccGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGc                                                                                              >  2:2150188/1‑148 (MQ=255)
                                                   gTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTa                                                                                           >  1:854432/1‑148 (MQ=255)
                                                   gTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTa                                                                                           <  1:3654709/148‑1 (MQ=255)
                                                     tCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGc                                                                                         >  1:2877704/1‑148 (MQ=255)
                                                     tCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGc                                                                                         >  2:2431758/1‑148 (MQ=255)
                                                     tCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGc                                                                                         >  2:1208069/1‑148 (MQ=255)
                                                         ggCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGAcc                                                                                     <  2:399965/148‑1 (MQ=255)
                                                           ccTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTg                                                                                   <  1:2673438/148‑1 (MQ=255)
                                                           ccTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTg                                                                                   <  1:61098/148‑1 (MQ=255)
                                                              tCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGa                                                                                <  2:2339315/148‑1 (MQ=255)
                                                                 tCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaa                                                                             <  1:184073/148‑1 (MQ=255)
                                                                  cAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                            <  2:450694/148‑1 (MQ=255)
                                                                  cAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                            <  2:2605663/148‑1 (MQ=255)
                                                                   aGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAg                                                                           <  2:2843740/148‑1 (MQ=255)
                                                                   aGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAg                                                                           <  2:2843515/148‑1 (MQ=255)
                                                                   aGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAg                                                                           <  2:756310/148‑1 (MQ=255)
                                                                    gCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAgg                                                                          >  2:2133182/1‑148 (MQ=255)
                                                                     cGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAggg                                                                         >  2:964137/1‑148 (MQ=255)
                                                                      gATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGc                                                                        <  1:1198093/148‑1 (MQ=255)
                                                                       aTCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCt                                                                       >  1:352657/1‑148 (MQ=255)
                                                                       aTCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCt                                                                       >  1:352652/1‑148 (MQ=255)
                                                                       aTCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCt                                                                       >  1:535573/1‑148 (MQ=255)
                                                                        tCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTg                                                                      <  1:1859648/148‑1 (MQ=255)
                                                                         ccGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGc                                                                     >  1:248472/1‑148 (MQ=255)
                                                                          cGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGcc                                                                    <  1:1618995/148‑1 (MQ=255)
                                                                             gCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCAGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGc                                                                 >  2:3412455/1‑148 (MQ=255)
                                                                               gACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGcca                                                               <  1:3268623/148‑1 (MQ=255)
                                                                                  gggCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccacca                                                            <  1:3416428/148‑1 (MQ=255)
                                                                                    gcgcTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATg                                                          <  2:1654147/148‑1 (MQ=255)
                                                                                    gcgcTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATg                                                          <  2:1263133/148‑1 (MQ=255)
                                                                                      gcTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGAt                                                        <  2:3331182/148‑1 (MQ=255)
                                                                                          gCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTgcg                                                    <  1:1489601/148‑1 (MQ=255)
                                                                                          gCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTgcg                                                    <  1:2150188/148‑1 (MQ=255)
                                                                                           cAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCGCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTgcgc                                                   <  1:202/148‑1 (MQ=255)
                                                                                           cAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTgcgc                                                   <  1:1611281/148‑1 (MQ=255)
                                                                                           cAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTgcgc                                                   <  1:2260787/148‑1 (MQ=255)
                                                                                              ggCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTgg                                                >  2:1109398/1‑148 (MQ=255)
                                                                                              ggCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTgg                                                >  1:2147887/1‑148 (MQ=255)
                                                                                              ggCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTgg                                                >  1:217698/1‑148 (MQ=255)
                                                                                                    cacgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATGGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGa                                          <  2:1368276/148‑1 (MQ=255)
                                                                                                    cacgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGa                                          >  2:3066063/1‑148 (MQ=255)
                                                                                                    cacgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGa                                          >  2:1161303/1‑148 (MQ=255)
                                                                                                    cacgGTATGTTCGAGTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGa                                          <  2:1368212/148‑1 (MQ=255)
                                                                                                      cgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATg                                        <  1:1840190/148‑1 (MQ=255)
                                                                                                      cgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATg                                        >  2:2257458/1‑148 (MQ=255)
                                                                                                      cgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATg                                        >  2:3744403/1‑148 (MQ=255)
                                                                                                            gTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGAc                                  >  2:1370843/1‑148 (MQ=255)
                                                                                                             ttCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGAcc                                 <  1:2955672/148‑1 (MQ=255)
                                                                                                             ttCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGAcc                                 <  1:1044979/148‑1 (MQ=255)
                                                                                                               cGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTg                               <  1:1021289/148‑1 (MQ=255)
                                                                                                               cGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTg                               <  1:1021639/148‑1 (MQ=255)
                                                                                                                  ttCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCg                            >  1:1001001/1‑148 (MQ=255)
                                                                                                                  ttCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATAGCGCAGGCCGCGATGCACGACCTGCCg                            >  1:2236348/1‑148 (MQ=255)
                                                                                                                   tCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGa                           >  1:1459289/1‑148 (MQ=255)
                                                                                                                   tCCCGGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGa                           <  1:259779/148‑1 (MQ=255)
                                                                                                                    cccTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGAc                          >  2:14181/1‑148 (MQ=255)
                                                                                                                    cccTGCAGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGAc                          >  2:1684492/1‑148 (MQ=255)
                                                                                                                      cTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTa                        >  2:833256/1‑148 (MQ=255)
                                                                                                                       tGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTag                       >  2:2154832/1‑147 (MQ=255)
                                                                                                                            tACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTAGTCTgg                  >  2:3757349/1‑148 (MQ=255)
                                                                                                                             aCCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGt                 >  2:2060905/1‑148 (MQ=255)
                                                                                                                                   acgacgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcggg           >  1:673235/1‑148 (MQ=255)
                                                                                                                                    cgacgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggc          >  1:2622894/1‑148 (MQ=255)
                                                                                                                                    cgacgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggc          >  1:1944226/1‑148 (MQ=255)
                                                                                                                                      acgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggcgg        <  1:2689351/148‑1 (MQ=255)
                                                                                                                                      acgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggcgg        <  2:1770090/148‑1 (MQ=255)
                                                                                                                                            cgATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGAGTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGAc  >  1:1113215/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGAC  >  CP009273/279190‑279477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑