Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,525,215 A→C 8.7% L195R (CTG→CGG)  yhgE ← DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,525,2150AC 8.7% 392.9 / 5.4 179L195R (CTG→CGG) yhgEDUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (59/99);  new base C (19/2);  total (78/101)
Fisher's exact test for biased strand distribution p-value = 5.24e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

ATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCA  >  CP009273/3525068‑3525362
                                                                                                                                                   |                                                                                                                                                   
    cgcTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACGTGACTGTGGTAGCGCGAGGATAACCCCCAgcgc                                                                                                                                                 >  2:1681780/1‑148 (MQ=255)
      cTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCgg                                                                                                                                               >  1:3118557/1‑148 (MQ=255)
      cTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCgg                                                                                                                                               >  1:3118818/1‑148 (MQ=255)
           gCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATAACCGCCAGCGCGGTAAcc                                                                                                                                          >  2:1577222/1‑148 (MQ=255)
           gCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAAcc                                                                                                                                          >  2:1915094/1‑148 (MQ=255)
           gCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATAACCGCCAGCGCGGTAAcc                                                                                                                                          >  2:3804595/1‑148 (MQ=255)
               gaaaaCGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGAc                                                                                                                                      <  1:1681780/146‑1 (MQ=255)
               gTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGAc                                                                                                                                      <  2:1036022/148‑1 (MQ=255)
                     ggCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACg                                                                                                                                <  2:3568997/148‑1 (MQ=255)
                      gCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGt                                                                                                                               <  1:61359/148‑1 (MQ=255)
                      gCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGGAACCAGACCTAACGt                                                                                                                               >  2:3143561/1‑148 (MQ=255)
                       cAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTg                                                                                                                              <  1:1515589/148‑1 (MQ=255)
                         gggTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTgag                                                                                                                            <  2:1145023/148‑1 (MQ=255)
                         gggTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTgag                                                                                                                            <  1:432054/148‑1 (MQ=255)
                         gggTGATGATAAACATCAGGGTTAGCAATGATACTAACAGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACAGTGTTCGCGCGAGGATAACCGCCAGCGCGGTAAACACACCTAACCTgag                                                                                                                            >  2:667326/1‑148 (MQ=255)
                          ggTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCCGCGCGGTAACCAGACCTAACGTgaga                                                                                                                           >  2:3734547/1‑148 (MQ=255)
                           gTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGat                                                                                                                          <  2:1424510/148‑1 (MQ=255)
                             gatgatAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGataa                                                                                                                        <  2:816905/148‑1 (MQ=255)
                              atgatAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCACACCTAACGTGAGataaa                                                                                                                       >  2:50013/1‑148 (MQ=255)
                                gatAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGataaata                                                                                                                     <  1:811189/148‑1 (MQ=255)
                                gatAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGataaata                                                                                                                     <  1:540869/148‑1 (MQ=255)
                                 atAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTTAAAGTGGCTGTGGTCGCGCGAGGGTAACCGCCCGCGCGGGAACCAGACCGAAAGGGAGataaataa                                                                                                                    >  1:1067371/1‑148 (MQ=255)
                                 atAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGataaataa                                                                                                                    <  2:1744184/148‑1 (MQ=255)
                                 atAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGataaataa                                                                                                                    <  1:1394392/148‑1 (MQ=255)
                                  tAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGAtaaataaa                                                                                                                   <  2:2175874/148‑1 (MQ=255)
                                  tAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGAtaaataaa                                                                                                                   <  1:1190633/148‑1 (MQ=255)
                                   aaaCATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAAc                                                                                                                  <  1:3449354/148‑1 (MQ=255)
                                   aaaCATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATCACCGCCCGCGCGGTAAACAGACCGAACGTGAGATAAATAAAc                                                                                                                  >  1:1197980/1‑148 (MQ=255)
                                    aaCATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAAcc                                                                                                                 <  1:2584382/148‑1 (MQ=255)
                                    aaCATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAAcc                                                                                                                 <  1:1440278/148‑1 (MQ=255)
                                      cATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTTTCTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCGAAACTGAGATAAATAAAACaa                                                                                                               >  2:2963589/1‑147 (MQ=255)
                                         cAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCTATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCg                                                                                                            <  1:207341/148‑1 (MQ=255)
                                         cAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTTACCCGACCTAAAGTTAGAGAAATAAAACAGTCg                                                                                                            >  2:1104137/1‑148 (MQ=255)
                                         cAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAAAGTGAGATAAATAAACCACTCg                                                                                                            >  2:3307757/1‑148 (MQ=255)
                                         cAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACAGTGTTAGCGCGAGGATAACCCCCAGCCCGGTAACCAGACCAAAAGTGAGATAAATAAAACAGtag                                                                                                            >  1:884060/1‑146 (MQ=255)
                                         cAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATAACCGCCAGCGCGGTAACCCGACCTAACGGGAGATAAATAAACCACTCg                                                                                                            >  1:2877084/1‑148 (MQ=255)
                                         cAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAAAGTGACTGTGTTCGCGCGAGGATAACCGCCAGCGCGGGAACCCGACCTAACGTGTGATAAATAAACCAGTCg                                                                                                            >  1:2876441/1‑148 (MQ=255)
                                          aGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCgg                                                                                                           <  2:619796/148‑1 (MQ=255)
                                          aGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCgg                                                                                                           <  2:3410808/148‑1 (MQ=255)
                                            ggTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGtt                                                                                                         >  2:886424/1‑148 (MQ=255)
                                                     tGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGCGGATCACCCCCACCGCGGGAACCACACCAAACGGGAGTTAAATAAAACCGTCGGGTGCAAAAAag                                                                                                >  1:2308578/1‑148 (MQ=255)
                                                      gATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAaga                                                                                               <  2:2774054/148‑1 (MQ=255)
                                                      gATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGGGACTGTGTTCGCGCGAGGATAACCCCCAGCGCGGTTACCAGACCAAACGTGAGATAAATAAAACAGTCGGTTGCAAAAAaga                                                                                               >  2:1787428/1‑148 (MQ=255)
                                                      gATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTAGCGCGAGGATCAACGCCAGCGCGGTAACCAGACCTAAAGTGAGATAAATAAAACAGTCGGTTGCAAAAAaga                                                                                               >  2:3678897/1‑148 (MQ=255)
                                                      gATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACCTGACTGTGTTCGCGCGAGGATAACCGCCCCCGCGGTAAACCGACCAAAACTGTGAGAAATAAAACAATAGGTTGGaaaaaaca                                                                                               >  1:2786827/1‑146 (MQ=255)
                                                           tAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTa                                                                                          <  2:2648178/148‑1 (MQ=255)
                                                            aaCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGCCTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCGAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTAt                                                                                         >  2:3084263/1‑148 (MQ=255)
                                                            aaCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACGGGACTGTGTTCGCGCGCGGATCACCGCCCCCGCGGTAACCCGACCAAACGGGAGGTAAATAAACCAGTCGGGTGGAAAAAAGAGCGGAt                                                                                         >  1:3372052/1‑148 (MQ=255)
                                                             aCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAAAGTGAGATAAATAAAACAGTCGGGTGCAAAAAAGAGCGGAtt                                                                                        >  1:1928514/1‑148 (MQ=255)
                                                                gcagcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACGTGACTGTGTTGGCGCGCGGATCACCGCCCGCGCGGGAACCAGACCTAACGTGAGATAAAAAAACCAGTCGGGTGCAAAAAAGAGCGGATTgta                                                                                     >  1:2449006/1‑146 (MQ=255)
                                                                   gcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGaaaaa                                                                                  <  1:3391656/148‑1 (MQ=255)
                                                                   gcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGaaaaa                                                                                  <  1:2062772/148‑1 (MQ=255)
                                                                   gcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTTAACGTGACAGTGTTGGCGCGAGGATAACCCCCCCCCCGGTAACCAGACCGAAACTGGGATAAAAAAAACAGTCGGGTTCAAAAAAAATCGGATTTtaaaa                                                                                  >  1:1731944/1‑148 (MQ=255)
                                                                       ccccGTGGCGATTAACGTTAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTg                                                                              <  1:1259524/148‑1 (MQ=255)
                                                                       ccccGTGGCGATTAACGTTAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTg                                                                              <  1:199724/148‑1 (MQ=255)
                                                                       ccccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACATAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTg                                                                              <  1:2381901/148‑1 (MQ=255)
                                                                       ccccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAAAGTGACTGTGTTAGCGCGAGGATCACCGCCAGCGCGGTAACCCGACCTAACCTGAGATAAATAAAACAGTCGGTTGCAAAAAATAGCGTATTGTAAAACGTg                                                                              >  2:1382187/1‑148 (MQ=255)
                                                                           gTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGc                                                                          <  2:1489011/148‑1 (MQ=255)
                                                                              gCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCcaa                                                                       <  1:172085/148‑1 (MQ=255)
                                                                                 aTTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCCCCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCcaacaa                                                                    >  2:1526301/1‑148 (MQ=255)
                                                                                  ttAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAAGGTGATATAAATAAACCAGTCGGTTGCACAAAAGAGCGTATTGAAAAACGTGATGCCAACAAg                                                                   <  2:1236608/148‑1 (MQ=255)
                                                                                  ttAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAg                                                                   <  1:1385400/148‑1 (MQ=255)
                                                                                  ttAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGGGGCTCTTGTCGCGCGAGGATCACCGCCACCGAGGTAAACATAACTAACCGGTGAAAAATAAAACAGTGGGTTTCTAAAAAGAGCGTATTGAAAAAAGTGATGcacaaaag                                                                   >  2:1403285/1‑141 (MQ=255)
                                                                                   tAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGCTCACCGCCCGCGCGGTAACCAGACCTAACGTGAGATAAATAAAACAGTCGGTTGCAAAAAATAGCGTATTGAAAAAACTGTTTCCCACAAgg                                                                  >  2:354125/1‑147 (MQ=255)
                                                                                   tAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGt                                                                  <  2:1635083/148‑1 (MQ=255)
                                                                                   tAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTAGCGCGAGGATAAACCCCCGCGCCGTAAACAGAACTAAAGTGAGATAAAAAAACCACTCGGGTGAAAAAAAAAGCGTATTTAAAAAACAGATGCcaacaatt                                                                  >  2:2279436/1‑146 (MQ=255)
                                                                                   tAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGTTCACCCCCAGCGCGGTAACCAGACCTAAAGTGAGATAAAAAAAACAGTCGGGTGCAAAAAAGAGCGTATTTAAAAAAGTGATGCccacaaat                                                                  >  1:1272457/1‑146 (MQ=255)
                                                                                   tAACGTGAAAAACTTTTTAATAGATTCTATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCTGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGt                                                                  <  1:887293/148‑1 (MQ=255)
                                                                                      cGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTa                                                               <  1:3503896/148‑1 (MQ=255)
                                                                                       gTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTaa                                                              <  2:3147731/148‑1 (MQ=255)
                                                                                       gTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTaa                                                              <  2:3147722/148‑1 (MQ=255)
                                                                                       gTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTaa                                                              <  1:1862435/148‑1 (MQ=255)
                                                                                        tGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTaaa                                                             <  1:1577222/148‑1 (MQ=255)
                                                                                        tGAACAACTTTTGAATAGAGTCGATTAAAAGTGACATTGTTCGCGCGAGGAAAACCCCCAGCGCGGTAAACAGACCAAACGTGAGATAAATAAAACAGTCGGTTGCAAAAAAATGCGTAATGAAAAAAGTTATTCCAACAAGTTTaaa                                                             >  2:1740580/1‑148 (MQ=255)
                                                                                                ttttGAATAGAGTCGATTAAACGTGACTGTGTTAGCGCCAGGATAACCGCCAGCGCGGTAACCAGACCTAAAGTGAGATAAATAAAACAGTCGGTTTCAAAAAATAGCGTAATTAAAAAAGTGATGCCAACAACTTTAaacaaaaaaa                                                     >  2:277105/1‑143 (MQ=255)
                                                                                                       tAGAGTCGATTACACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCACCAAGTTTAAACAACTCACTCCACAg                                              <  2:3354702/148‑1 (MQ=255)
                                                                                                        atagTCTATTAAACGTGACTGTGTTCGCGCGAGGATCCCCGCCAGCGCGTTAACCAGACCTAACGTTATATAAAAAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGt                                             <  1:1104137/146‑1 (MQ=255)
                                                                                                        agagTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTACCGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGt                                             <  1:3307757/148‑1 (MQ=255)
                                                                                                           gTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTaaa                                          >  2:2696607/1‑148 (MQ=255)
                                                                                                            tCGATTAAAGGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTaaaa                                         <  2:2255196/148‑1 (MQ=255)
                                                                                                                ttAAACGTGAGTGTGTTCGCGCGAGGATCACCGCCCGCGCGTTAACCAGACCTAACGTGATATAAATAAACCAGTCGGTTGAAAAAAAGAGCGTATTGAAAAACGTTATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAc                                     <  1:1964481/148‑1 (MQ=255)
                                                                                                                 tAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAcc                                    >  2:2525989/1‑148 (MQ=255)
                                                                                                                   aaCGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAg                                  >  1:112426/1‑148 (MQ=255)
                                                                                                                    aCGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGATATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGc                                 <  2:3118818/148‑1 (MQ=255)
                                                                                                                     cGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGcc                                >  2:1833611/1‑148 (MQ=255)
                                                                                                                         aCTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGt                            >  1:3166871/1‑148 (MQ=255)
                                                                                                                           tgtgTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCa                          <  1:1520899/148‑1 (MQ=255)
                                                                                                                              gtTCGCGCGAGGAACACCCCCAGCGCGTTAACCAGACCTAACGTTAGATAAATAAACCCGTGGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCAAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGc                       <  1:681361/148‑1 (MQ=255)
                                                                                                                                 cgcgcgAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCAt                    <  2:603785/148‑1 (MQ=255)
                                                                                                                                  gcgcgAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCAtt                   <  1:1276910/148‑1 (MQ=255)
                                                                                                                                  gcgcgAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCAtt                   <  2:808084/148‑1 (MQ=255)
                                                                                                                                  gcgcgAGGACCCCCGCCCGCGCGGTACCCAGACCTAACGTGAGATAAATAACCCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCAtt                   <  1:1648686/148‑1 (MQ=255)
                                                                                                                                     cgAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGc                >  2:1524038/1‑148 (MQ=255)
                                                                                                                                      gAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCg               <  2:2004107/148‑1 (MQ=255)
                                                                                                                                      gAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCg               <  2:2308578/148‑1 (MQ=255)
                                                                                                                                         gATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCgaga            >  1:446181/1‑148 (MQ=255)
                                                                                                                                           tCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGaaa          >  1:2255897/1‑148 (MQ=255)
                                                                                                                                            ccccGCCAGCGCGGTAACCACACCAAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGaaaa         <  1:2685897/146‑1 (MQ=255)
                                                                                                                                             aCCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGaaaaa        <  2:137246/148‑1 (MQ=255)
                                                                                                                                                  cAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCAcc   <  2:1967931/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCA  >  CP009273/3525068‑3525362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑