Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,528,406 G→T 100% I274I (ATC→ATA)  envZ ← sensory histidine kinase in two‑component regulatory system with OmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,528,4060GT97.9% 641.7 / 3.5 207I274I (ATC→ATA) envZsensory histidine kinase in two‑component regulatory system with OmpR
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (115/87);  total (118/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 211 total aligned reads displayed.

GCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACA  >  CP009273/3528261‑3528550
                                                                                                                                                 |                                                                                                                                                
gcgcGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTat                                                                                                                                                <  1:2546256/148‑1 (MQ=255)
 cgcgGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCtata                                                                                                                                               <  1:2861349/148‑1 (MQ=255)
      tttCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCttt                                                                                                                                          <  1:2775755/148‑1 (MQ=255)
       ttCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTa                                                                                                                                         <  2:2655099/148‑1 (MQ=255)
          aaTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTg                                                                                                                                      <  1:3430128/148‑1 (MQ=255)
          aaTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTg                                                                                                                                      <  1:1529915/148‑1 (MQ=255)
          aaTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTg                                                                                                                                      <  1:240842/148‑1 (MQ=255)
           aTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGa                                                                                                                                     >  1:1020783/1‑148 (MQ=255)
           aTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGa                                                                                                                                     <  2:3741769/148‑1 (MQ=255)
            tttCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTATTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGAt                                                                                                                                    >  1:416338/1‑148 (MQ=255)
             ttCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATc                                                                                                                                   >  1:3168286/1‑148 (MQ=255)
                 cGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGAtt                                                                                                                               <  2:3353536/148‑1 (MQ=255)
                 cGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGAtt                                                                                                                               >  2:2294771/1‑148 (MQ=255)
                 cGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGAtt                                                                                                                               <  1:557322/148‑1 (MQ=255)
                  tcTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTc                                                                                                                              <  2:2342130/147‑1 (MQ=255)
                  gCTCAGAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTc                                                                                                                              <  2:765503/148‑1 (MQ=255)
                   cTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCt                                                                                                                             >  2:35104/1‑148 (MQ=255)
                   cTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCt                                                                                                                             >  2:1860645/1‑148 (MQ=255)
                     cATAGCCACTATCGGCAGCAAACACCTCAAAGAGTACTGAATTAATATCCGCCATTTCCAACTGCATCTCCTGCCCGGTGCGCATGTATTCTATAAAAAGCTCAAACATGTAGTTGAACTCTTATATATCTTAATTGTTCAAttaagc                                                                                                                           >  2:2525495/1‑144 (MQ=255)
                       tAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCa                                                                                                                         >  2:408966/1‑148 (MQ=255)
                            aCTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAg                                                                                                                    <  1:3233503/148‑1 (MQ=255)
                            aCTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAg                                                                                                                    >  2:1622851/1‑148 (MQ=255)
                             cTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGc                                                                                                                   >  2:2973484/1‑148 (MQ=255)
                                tCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCAt                                                                                                                <  2:2447378/148‑1 (MQ=255)
                                 cGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATc                                                                                                               <  1:3361242/148‑1 (MQ=255)
                                          tcacctcaccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTgctcgct                                                                                                      <  2:1772253/148‑1 (MQ=255)
                                          tcacctcaccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTgctcgct                                                                                                      >  2:221076/1‑148 (MQ=255)
                                            acctcaccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCtca                                                                                                    >  2:2128024/1‑148 (MQ=255)
                                             cctcaccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCtcat                                                                                                   >  2:1209447/1‑148 (MQ=255)
                                             cctcaccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCtcat                                                                                                   <  1:2322795/148‑1 (MQ=255)
                                              ctcaccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCtcatc                                                                                                  <  2:1979015/148‑1 (MQ=255)
                                                caccGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTcatcat                                                                                                >  2:1574544/1‑148 (MQ=255)
                                                    gAGGACGGCATTAAGATCCGCCATTTCCATCGGCATCTCCGGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCa                                                                                            <  2:2478904/148‑1 (MQ=255)
                                                     aGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAg                                                                                           >  1:1071638/1‑148 (MQ=255)
                                                       tACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTc                                                                                         >  2:447044/1‑148 (MQ=255)
                                                       tACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGAGTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTc                                                                                         >  2:2091075/1‑148 (MQ=255)
                                                        aCTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCg                                                                                        >  1:3109400/1‑148 (MQ=255)
                                                             aTTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAgg                                                                                   <  2:65622/148‑1 (MQ=255)
                                                               tAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCg                                                                                 >  2:2216700/1‑148 (MQ=255)
                                                                  gATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAAt                                                                              <  1:1506217/148‑1 (MQ=255)
                                                                   aTCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGACTa                                                                             >  2:3053307/1‑148 (MQ=255)
                                                                   aTCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATa                                                                             >  2:3197247/1‑148 (MQ=255)
                                                                   aTCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATa                                                                             >  2:1146569/1‑148 (MQ=255)
                                                                   aTCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATa                                                                             >  2:3198788/1‑148 (MQ=255)
                                                                     ccGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATAcg                                                                           <  1:77297/148‑1 (MQ=255)
                                                                     ccGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATAcg                                                                           <  1:495031/148‑1 (MQ=255)
                                                                        ccATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGt                                                                        <  1:3402226/148‑1 (MQ=255)
                                                                        ccATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGt                                                                        <  1:3278586/148‑1 (MQ=255)
                                                                        ccATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGt                                                                        <  1:1622851/148‑1 (MQ=255)
                                                                          aTTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCa                                                                      >  1:1764907/1‑148 (MQ=255)
                                                                          aTTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCa                                                                      <  2:1319308/148‑1 (MQ=255)
                                                                           tttCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAg                                                                     >  1:3002847/1‑148 (MQ=255)
                                                                           tttCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAg                                                                     >  1:3002402/1‑148 (MQ=255)
                                                                            ttCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAgc                                                                    >  1:269882/1‑148 (MQ=255)
                                                                            ttCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAgc                                                                    >  2:1146358/1‑148 (MQ=255)
                                                                              ccATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAgcgg                                                                  <  1:1927011/148‑1 (MQ=255)
                                                                              ccATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAgcgg                                                                  <  1:3079645/148‑1 (MQ=255)
                                                                                    gCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTgcg                                                            >  1:1436510/1‑148 (MQ=255)
                                                                                    gCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTgcg                                                            >  1:2148665/1‑148 (MQ=255)
                                                                                     cATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTgcgc                                                           >  2:2235991/1‑148 (MQ=255)
                                                                                      aTCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCa                                                          <  1:1191184/148‑1 (MQ=255)
                                                                                      aTCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCa                                                          <  1:2068758/148‑1 (MQ=255)
                                                                                       tctcCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCaa                                                         >  1:8930/1‑148 (MQ=255)
                                                                                       tctcCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCaa                                                         >  1:8839/1‑148 (MQ=255)
                                                                                        ctcCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAg                                                        >  2:1876849/1‑148 (MQ=255)
                                                                                        ctcCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAg                                                        >  2:1742923/1‑148 (MQ=255)
                                                                                         tcCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGt                                                       >  2:2662767/1‑148 (MQ=255)
                                                                                           cTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCg                                                     <  1:2637815/148‑1 (MQ=255)
                                                                                             gCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTg                                                   <  1:1372411/148‑1 (MQ=255)
                                                                                              cccGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGa                                                  <  2:1940073/148‑1 (MQ=255)
                                                                                                          gTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGcc                                      >  1:2506323/1‑148 (MQ=255)
                                                                                                            aGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCAt                                    <  2:3168285/148‑1 (MQ=255)
                                                                                                                  tAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATcagcag                              >  1:3632806/1‑148 (MQ=255)
                                                                                                                  tAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATcagcag                              >  2:3495010/1‑148 (MQ=255)
                                                                                                                  tAAACTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATcagcag                              >  1:3819239/1‑148 (MQ=255)
                                                                                                                   aaaCTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCagcagc                             >  1:864540/1‑148 (MQ=255)
                                                                                                                    aaCTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCg                            >  1:650938/1‑148 (MQ=255)
                                                                                                                      cTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTg                          >  2:904098/1‑148 (MQ=255)
                                                                                                                      cTGCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTg                          >  1:3191505/1‑148 (MQ=255)
                                                                                                                        gCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCg                        >  2:2469988/1‑148 (MQ=255)
                                                                                                                        gCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCg                        >  1:1862248/1‑148 (MQ=255)
                                                                                                                        gCTCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCg                        >  2:549914/1‑148 (MQ=255)
                                                                                                                          tCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGAGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGt                      >  2:1354894/1‑148 (MQ=255)
                                                                                                                          tCAATGATGGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCCCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGAGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGt                      >  2:3259225/1‑148 (MQ=255)
                                                                                                                             atgatgGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCAt                   <  1:2241064/148‑1 (MQ=255)
                                                                                                                             atgatgGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCAt                   <  1:2905833/148‑1 (MQ=255)
                                                                                                                             atgatgGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCAt                   <  2:1850395/148‑1 (MQ=255)
                                                                                                                              tgatgGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATc                  >  1:3807038/1‑148 (MQ=255)
                                                                                                                              tgatgGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATc                  >  1:1049430/1‑148 (MQ=255)
                                                                                                                              tgatgGCGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATc                  >  2:838348/1‑148 (MQ=255)
                                                                                                                                    cGTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAg            >  2:687220/1‑148 (MQ=255)
                                                                                                                                     gTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGt           <  1:221076/148‑1 (MQ=255)
                                                                                                                                     gTTGCACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCACCATCAGCAGCGTGCGGTCATCCGCCAGt           <  2:3371335/148‑1 (MQ=255)
                                                                                                                                     gTTGCACTCTTCTATATCTTTATTGATCGAGTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGt           <  2:218348/148‑1 (MQ=255)
                                                                                                                                         cACTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCt       >  1:3732647/1‑148 (MQ=255)
                                                                                                                                          aCTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCtt      >  2:3077958/1‑148 (MQ=255)
                                                                                                                                          aCTCTTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCtt      >  2:53582/1‑148 (MQ=255)
                                                                                                                                           ctctTCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTa     >  2:2813114/1‑148 (MQ=255)
                                                                                                                                            tcttctATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTaa    >  1:665200/1‑148 (MQ=255)
                                                                                                                                              ttCTATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAaca  >  1:1035049/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACA  >  CP009273/3528261‑3528550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑