Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,544,164 G→T 13.7% R557S (CGT→AGT)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,544,1640GT13.7% 933.1 / 80.0 298R557S (CGT→AGT) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (107/149);  new base T (19/22);  total (127/171)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 298 total aligned reads displayed.

CTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACGGACTT  >  CP009273/3544018‑3544310
                                                                                                                                                  |                                                                                                                                                  
cTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGa                                                                                                                                                   >  2:2023683/1‑148 (MQ=255)
   aTCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAAtt                                                                                                                                                >  2:934727/1‑148 (MQ=255)
    tCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAAttt                                                                                                                                               <  1:1018333/148‑1 (MQ=255)
      gCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCt                                                                                                                                             <  1:3448302/148‑1 (MQ=255)
        cAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCttt                                                                                                                                           <  1:1146899/148‑1 (MQ=255)
          aCCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGt                                                                                                                                         >  1:1843844/1‑148 (MQ=255)
             aGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACa                                                                                                                                      >  2:480737/1‑148 (MQ=255)
              gCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACaa                                                                                                                                     >  1:83365/1‑148 (MQ=255)
              gCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACaa                                                                                                                                     <  1:2029761/148‑1 (MQ=255)
              gCGGATCGTTGTTGATAACGTCAGCCACTTTGTTGATAGCAAAGATAATAATCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCCAACAGGAACACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACCAATTTCTTTGTaaaa                                                                                                                                     >  2:1417796/1‑148 (MQ=255)
                   tCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCa                                                                                                                                >  2:2159253/1‑148 (MQ=255)
                    cGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAg                                                                                                                               >  2:3689100/1‑148 (MQ=255)
                      ttgttgATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGaa                                                                                                                             <  1:696530/148‑1 (MQ=255)
                         ttgATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAatat                                                                                                                          <  2:2227902/148‑1 (MQ=255)
                          tgATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATg                                                                                                                         >  2:2674552/1‑148 (MQ=255)
                           gATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATgc                                                                                                                        <  1:3063243/148‑1 (MQ=255)
                           gATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATgc                                                                                                                        <  2:1111971/148‑1 (MQ=255)
                            aTCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATgca                                                                                                                       <  1:2469211/148‑1 (MQ=255)
                             tCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATgcag                                                                                                                      >  2:1230690/1‑148 (MQ=255)
                                   cAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTATCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGAtt                                                                                                                >  1:1033407/1‑148 (MQ=255)
                                     gCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCa                                                                                                              >  1:3583707/1‑148 (MQ=255)
                                     gCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCa                                                                                                              <  1:3196526/148‑1 (MQ=255)
                                      ccACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAg                                                                                                             >  1:804271/1‑148 (MQ=255)
                                      ccACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAg                                                                                                             <  1:2891147/148‑1 (MQ=255)
                                       cACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATGTCTTTGTACAACGCCAGAATATGCAGCAGATTCAgg                                                                                                            <  2:1656923/148‑1 (MQ=255)
                                       cACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAgg                                                                                                            <  1:2364453/148‑1 (MQ=255)
                                       cACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAgg                                                                                                            >  2:1317335/1‑148 (MQ=255)
                                       cACTTGGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAgg                                                                                                            <  1:3540386/148‑1 (MQ=255)
                                        aCTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGt                                                                                                           >  2:2386564/1‑148 (MQ=255)
                                         cTTTGTTGATCGCAAAGATAATATTCGTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTg                                                                                                          <  2:3165104/148‑1 (MQ=255)
                                          tttGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGc                                                                                                         >  2:1864795/1‑148 (MQ=255)
                                               tgATCGCAAAGATAATATTCTTCGCCAGTTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCg                                                                                                    <  1:3020385/148‑1 (MQ=255)
                                               tgATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGATGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCg                                                                                                    <  2:355591/148‑1 (MQ=255)
                                               tgATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCg                                                                                                    <  2:675198/148‑1 (MQ=255)
                                               tgATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCg                                                                                                    <  2:1259235/148‑1 (MQ=255)
                                                 aTCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGtt                                                                                                  >  1:640039/1‑148 (MQ=255)
                                                 aTCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGtt                                                                                                  <  2:2501990/148‑1 (MQ=255)
                                                  tCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGttt                                                                                                 >  1:2121012/1‑148 (MQ=255)
                                                  tCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGttt                                                                                                 <  1:144698/148‑1 (MQ=255)
                                                  tCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGttt                                                                                                 <  1:3473470/148‑1 (MQ=255)
                                                  tCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGttt                                                                                                 <  1:2972039/148‑1 (MQ=255)
                                                    gCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGt                                                                                               <  1:662117/148‑1 (MQ=255)
                                                     cAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTa                                                                                              <  1:1392121/148‑1 (MQ=255)
                                                        aGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTc                                                                                           <  2:1284259/148‑1 (MQ=255)
                                                        aGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTc                                                                                           <  1:2917777/148‑1 (MQ=255)
                                                         gATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCa                                                                                          >  2:1944634/1‑148 (MQ=255)
                                                         gATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCa                                                                                          >  2:1700773/1‑148 (MQ=255)
                                                           taataTTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATg                                                                                        <  1:990560/148‑1 (MQ=255)
                                                              tatTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCaa                                                                                     <  1:2675464/148‑1 (MQ=255)
                                                              tatTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCaa                                                                                     <  2:3477989/148‑1 (MQ=255)
                                                              tatTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCaa                                                                                     >  2:3437936/1‑148 (MQ=255)
                                                              tatTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCaa                                                                                     >  1:135017/1‑148 (MQ=255)
                                                               atTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCaaa                                                                                    <  1:3659206/148‑1 (MQ=255)
                                                               atTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCaaa                                                                                    >  2:150465/1‑148 (MQ=255)
                                                                ttcttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGt                                                                                                   <  2:1876841/132‑1 (MQ=255)
                                                                 tcttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACg                                                                                  <  2:3689698/148‑1 (MQ=255)
                                                                 tcttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACg                                                                                  <  1:1576322/148‑1 (MQ=255)
                                                                  cttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGt                                                                                 >  2:2513177/1‑148 (MQ=255)
                                                                   ttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACggt                                                                                >  2:672827/1‑146 (MQ=255)
                                                                      gCCAGGTAGTAGCCCGGTGCAGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGTGGGTTTTCACGAATTTCTTTGTACAACGCCAGTATATGCTGAAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGa                                                                             <  2:1646250/148‑1 (MQ=255)
                                                                       ccAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGAt                                                                            <  2:615396/148‑1 (MQ=255)
                                                                       ccAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGAt                                                                            <  2:3228234/148‑1 (MQ=255)
                                                                       ccAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGAt                                                                            <  1:241880/148‑1 (MQ=255)
                                                                         aGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCt                                                                          >  2:2547960/1‑148 (MQ=255)
                                                                          ggTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGGTTGTACTCATGCAAACGTTTGATCTg                                                                         >  1:2844855/1‑148 (MQ=255)
                                                                          ggTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTGTGTTCTCAAGCACACCTTTTATATg                                                                         >  1:1638594/1‑148 (MQ=255)
                                                                           gtagtaGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGa                                                                        <  2:2328512/148‑1 (MQ=255)
                                                                            tagtagCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTTATCTGaa                                                                       >  1:422952/1‑148 (MQ=255)
                                                                            tagtagCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGaa                                                                       <  2:1075309/148‑1 (MQ=255)
                                                                            tagtagCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGaa                                                                       <  2:3476892/148‑1 (MQ=255)
                                                                             agtagCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAat                                                                      <  1:92726/148‑1 (MQ=255)
                                                                               tagCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAatat                                                                    <  2:91855/148‑1 (MQ=255)
                                                                                  cccGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGa                                                                 >  1:1907144/1‑148 (MQ=255)
                                                                                     ggTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCAAGCAAAAGTTTGATCTGAATATAGaaaa                                                              >  1:1093825/1‑148 (MQ=255)
                                                                                      gTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAAt                                                             <  1:1516759/148‑1 (MQ=255)
                                                                                      gTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAAt                                                             <  1:1772017/148‑1 (MQ=255)
                                                                                         ccGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGc                                                          >  1:3268980/1‑148 (MQ=255)
                                                                                         ccGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGc                                                          <  1:769419/148‑1 (MQ=255)
                                                                                           gCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCAAGCAAACGTTTGATCTGAATATCGAAAATCGCCt                                                        >  1:730089/1‑148 (MQ=255)
                                                                                                cgcgCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGa                                                   <  1:2535060/148‑1 (MQ=255)
                                                                                                 gcgcCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGAt                                                  <  2:2997070/148‑1 (MQ=255)
                                                                                                    ccGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGa                                               <  2:2149230/148‑1 (MQ=255)
                                                                                                       aaGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGAtct                                            <  1:1271620/148‑1 (MQ=255)
                                                                                                       aaGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTGTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGAtct                                            >  1:1182508/1‑148 (MQ=255)
                                                                                                        agagGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGAtctc                                           <  2:521133/148‑1 (MQ=255)
                                                                                                         gagGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCa                                          >  2:1159632/1‑148 (MQ=255)
                                                                                                          agGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCaa                                         <  2:3761823/148‑1 (MQ=255)
                                                                                                           ggAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATTCAAACGTTTGATCNGAATATAGAAAATAGCCTGTGGATTGTTCTCAAt                                        >  1:1432325/1‑148 (MQ=255)
                                                                                                           ggAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAAt                                        >  1:3779167/1‑148 (MQ=255)
                                                                                                            gAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATa                                       <  2:2121011/148‑1 (MQ=255)
                                                                                                                     cgGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGa                              <  1:1230266/148‑1 (MQ=255)
                                                                                                                        tACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACt                           <  2:1544166/148‑1 (MQ=255)
                                                                                                                         aCGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACtt                          <  2:2829593/148‑1 (MQ=255)
                                                                                                                                  gCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACt                 <  1:2102585/148‑1 (MQ=255)
                                                                                                                                     tGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCg              <  1:2021386/148‑1 (MQ=255)
                                                                                                                                            tttCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACg       >  2:2716357/1‑148 (MQ=255)
                                                                                                                                             ttCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACgg      <  1:1858373/148‑1 (MQ=255)
                                                                                                                                               cACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACGGAc    <  1:3689100/148‑1 (MQ=255)
                                                                                                                                               cACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACGGAc    <  1:333101/148‑1 (MQ=255)
                                                                                                                                                aCGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACGGACt   <  2:1028160/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACGGACTT  >  CP009273/3544018‑3544310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑