Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,546,300 G→T 11.1% intergenic (‑468/‑144) malP ← / → malT maltodextrin phosphorylase/mal regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,546,3000GT11.1% 877.3 / 16.4 301intergenic (‑468/‑144)malP/malTmaltodextrin phosphorylase/mal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (77/181);  new base T (41/2);  total (118/183)
Fisher's exact test for biased strand distribution p-value = 6.11e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

ATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTAACTATGC  >  CP009273/3546154‑3546447
                                                                                                                                                  |                                                                                                                                                   
aTAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTgg                                                                                                                                                    <  2:963866/148‑1 (MQ=255)
  aGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGaa                                                                                                                                                  <  2:2699857/148‑1 (MQ=255)
    aTTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAATATTTGGAAtt                                                                                                                                                >  2:3526370/1‑148 (MQ=255)
      ttattaAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTAGGTGAGTTGTTAATAAAGATTTTGAATtgt                                                                                                                                              >  1:290617/1‑148 (MQ=255)
          taAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTTGGTGTGTTGTTAATAAAGATTTTGTATtttgtaa                                                                                                                                          >  1:1747656/1‑145 (MQ=255)
             gATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTATGTGAGTTGTTAATAAAGATTTGGTATTTTGACACAg                                                                                                                                       >  2:3336502/1‑148 (MQ=255)
             gATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAg                                                                                                                                       <  1:3809808/148‑1 (MQ=255)
             gATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAg                                                                                                                                       <  2:1056899/148‑1 (MQ=255)
             gATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAg                                                                                                                                       <  2:2962777/148‑1 (MQ=255)
              atcatcTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGt                                                                                                                                      >  2:1253112/1‑148 (MQ=255)
               tcatcTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTTGGTGTGTTGTTAATAAAGATTTTGTATTTTGTCACACTg                                                                                                                                     >  1:2612821/1‑148 (MQ=255)
                catcTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTTGGTGAGTTGTTAATAAAGAGTTGGACTTGTGACCCAGTGc                                                                                                                                    >  2:1312023/1‑148 (MQ=255)
                  tcTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTTGAATTGTGACACAGTGCaa                                                                                                                                  >  2:2174505/1‑148 (MQ=255)
                  tcTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTTGGTGAGTGGTTAATAAAGATTTGGAATTGTGACACAGTGCaa                                                                                                                                  >  2:1357285/1‑148 (MQ=255)
                   cTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCaaa                                                                                                                                 >  2:159111/1‑148 (MQ=255)
                    tttttAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATCAAGATTTGGAATTGTGACACAGTGCAAAt                                                                                                                                >  2:2332819/1‑148 (MQ=255)
                    tttttAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAAt                                                                                                                                <  1:2689286/148‑1 (MQ=255)
                       ttAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCa                                                                                                                             >  2:455689/1‑148 (MQ=255)
                       ttAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTAGGTGTGGTGTTAATTAAGTTTTGGAATTGTGACACAGTGCAAATTCa                                                                                                                             >  2:1763498/1‑148 (MQ=255)
                        tAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAg                                                                                                                            <  2:3003204/148‑1 (MQ=255)
                           aTGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGaca                                                                                                                         <  2:2279327/148‑1 (MQ=255)
                           aTGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGGGAGGTGTTAATAAATTTTTTGAATTGTGACACAGTGCAAATTAAGaca                                                                                                                         >  1:608756/1‑148 (MQ=255)
                             ggCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGAcaca                                                                                                                       >  2:1144016/1‑148 (MQ=255)
                             ggCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTAGGTGAGTTGTTAATAAATTTTTGGTATTGTTACACCGTGCAAATTAAGAcaca                                                                                                                       >  2:3612711/1‑148 (MQ=255)
                                  tttCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATaaaa                                                                                                                  <  1:3347475/148‑1 (MQ=255)
                                   ttCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTATGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATaaaaa                                                                                                                 >  2:1734274/1‑148 (MQ=255)
                                     catcaGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTTGGTGTGTTGTTTATAAATATTTGGTATTGTTACACACTGCAAAATCAGACACAaaaaaaaa                                                                                                               >  1:1061227/1‑148 (MQ=255)
                                     catcaGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATaaaaaaa                                                                                                               <  1:2040400/148‑1 (MQ=255)
                                      atcaGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAAc                                                                                                              >  1:2547993/1‑148 (MQ=255)
                                        caGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTTGGTGAGTTGTTAATTAAGATTTTGTATTGTGTCACCGTTCAAAATCAGACACATAAAAAAACgg                                                                                                            >  1:251913/1‑147 (MQ=255)
                                         agtaaTGAAGAATAAACCCTTGCTTAAATGAATCTTATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTc                                                                                                           <  1:3637050/145‑1 (MQ=255)
                                         aGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTATGTGAGTTGTTAATAAAGATTTTGAATTGTGACAAACTGCAAAATCAGACACATAAAAAAAAGTc                                                                                                           >  2:791197/1‑148 (MQ=255)
                                         aGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTc                                                                                                           >  2:1221578/1‑148 (MQ=255)
                                         aGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTc                                                                                                           <  1:2138261/148‑1 (MQ=255)
                                          ggAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCa                                                                                                          <  1:1776585/148‑1 (MQ=255)
                                            aaTGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTATGTGTGTTGTTAATAAAGATTTTGTATTGTGACACAGTGCAAAATAAGACACAAAAAAAAAAGTCaaa                                                                                                        >  1:3681732/1‑146 (MQ=255)
                                            aaTGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTAGGTGAGGTTTTAATAAAGATTTGGAATTGTGACACACTGCAAATTCAGACACAAAAAAAAACGTCATc                                                                                                        >  1:3442659/1‑148 (MQ=255)
                                             aTGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCg                                                                                                       <  1:3663856/148‑1 (MQ=255)
                                              tGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGc                                                                                                      <  2:2678433/148‑1 (MQ=255)
                                              tGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTAGGTGAGTTGTTAATAAAGATTTGGAAATGTGACACACTGCAAATTCACACACAAAAAAAAAACTCATCGc                                                                                                      >  1:3082300/1‑148 (MQ=255)
                                                  gaagaaACCCTTGCTTAAATGAATCTGATGAACATACGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGc                                                                                                  <  1:3560738/148‑1 (MQ=255)
                                                    agaaACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCAt                                                                                                <  2:1198222/148‑1 (MQ=255)
                                                       aaCCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGTGTTGTTAATAAAGATTTTGAATTTTGACACAGTGCAAAATAAGACACATAAAAAAAAGTCATACCATGCATTAg                                                                                             >  1:2186974/1‑148 (MQ=255)
                                                         cccTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGaa                                                                                           <  2:2774925/148‑1 (MQ=255)
                                                         cccTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGaa                                                                                           <  2:2877653/148‑1 (MQ=255)
                                                             tGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGt                                                                                       <  2:935256/148‑1 (MQ=255)
                                                             tGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGt                                                                                       <  2:740770/148‑1 (MQ=255)
                                                                 tAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCt                                                                                   <  1:1564905/148‑1 (MQ=255)
                                                                 tAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTTGGTTAGTTTTTTATAAAGTTTTTGTTTTTTGTGAAAGTGCCAATTAAAAAAAAAAAAAAAAATTAATAGCTTGGCTTATaaactttttt                                                                                   >  1:6958/1‑141 (MQ=255)
                                                                 tAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTTGGTGTGTTGTTTATAAATATTTGGAATTGTGACACACTGCAAAATAAGAAAAAAAAAAAAAACTAAAAGCTTTCATTATAAATGtttgt                                                                                   >  1:1971120/1‑146 (MQ=255)
                                                                 tAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGGTAGGTGGGTTGTTTATAAAGATTTTGTATTGTGTCACAGTTCAAAAAAACACACAAAAAAAAAACACATCGCGTGCAGTAAAAATGTTTCt                                                                                   >  1:2902399/1‑148 (MQ=255)
                                                                         tCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGAcc                                                                           <  2:3757665/148‑1 (MQ=255)
                                                                         tCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTTGGTGTGTTGTTTATAAAGATTTTGTATTGTTTAAAAGTGCAAATTAAGACACAAAAAAAAAACTCAAAGCGTGCAATAGAAAGGTTTGTGGCCGAcc                                                                           >  1:1681455/1‑148 (MQ=255)
                                                                          cTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCt                                                                          >  2:2224183/1‑148 (MQ=255)
                                                                          cTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTAGGTGGGTTGTTAATAAAGATTTGGTATTGTGGCACAGTGCAAATTAAGACACAAAAAAAAAACTCAAAGCGTGCATTAGAAAGGTTTATGGCCGACCt                                                                          >  2:648237/1‑148 (MQ=255)
                                                                              tgaACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTtata                                                                      <  1:2456989/148‑1 (MQ=255)
                                                                                 aCATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAAcc                                                                   <  2:580771/148‑1 (MQ=255)
                                                                                  cATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCa                                                                  <  1:3025112/148‑1 (MQ=255)
                                                                                    tAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCatt                                                                <  2:2346844/148‑1 (MQ=255)
                                                                                       gggAAACCAGTATTCACGCTGGATCAGCGTCGTTTTTGGTGGGTTGTTAATAAAGATTTTGAATTTTGACACAGTGCAAAATAAGACACCAAAAAAAAAGTCAACGCTTGCCTTATAAAGGTTTCTGGCCGACCATATAACCaataat                                                             >  2:344448/1‑148 (MQ=255)
                                                                                        ggAAACCAGTATTCACGCTGGATCAGCGTCGTGTTAGGTGAGTTGTTAATAAAGATTTGGTATTGTGACACAGTGCAAATTAAGACACAAAAAAAAAAGTCATAGCTTGCATTAGAAAGGTTTATGGGCGACCTTATAAACattaatt                                                            >  2:385954/1‑148 (MQ=255)
                                                                                         gAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCattaatta                                                           <  1:1449142/148‑1 (MQ=255)
                                                                                         gAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCattaatta                                                           <  1:455689/148‑1 (MQ=255)
                                                                                                    tCACGCTGGATCAGCGTCGTGTTAGGTGAGTTGTTAATAAAGATTTGGGATTGTGTCACAGTGCAAATTAAGACACATAAAAAAAAGTCAACGCTTGCATTAGAAATGTTTGTGGCCGACCGTATAACCATTAATTACGAAGCGCaaa                                                >  2:1518657/1‑148 (MQ=255)
                                                                                                         cTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCaaaaaaaa                                           <  1:1431179/148‑1 (MQ=255)
                                                                                                          tGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAaat                                          <  2:3668769/148‑1 (MQ=255)
                                                                                                          tGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAaat                                          <  2:2577566/148‑1 (MQ=255)
                                                                                                           ggATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAaata                                         <  2:3597834/148‑1 (MQ=255)
                                                                                                            gATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACAAAAAAAAAAGTCAACGCTTGCATTAGAAAGGTTTCTGGCCGACCGTATAACCATTAATTACGAAGCGCAAAAAAaataa                                        >  2:2531237/1‑148 (MQ=255)
                                                                                                            gATCAGCGTCGTGTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACACTGCAAATTAAGTCACAGAAAAAAACGTCATCGCGTGCATTAGAAAGGGTTCTGGCCGACCTTATAACCAATAATTACGAAGCGCAAAAAAaataa                                        >  2:902274/1‑148 (MQ=255)
                                                                                                                 gCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATAttt                                   <  1:18968/148‑1 (MQ=255)
                                                                                                                 gCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATAttt                                   <  1:1144016/148‑1 (MQ=255)
                                                                                                                 gCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATAttt                                   <  1:3594665/148‑1 (MQ=255)
                                                                                                                 gCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATAttt                                   <  1:1953042/148‑1 (MQ=255)
                                                                                                                  cgtcgtGTTAGGTGCGTTTTTTATAAAGAATTTGTATTTTTACCCAGTTCAAAATAATACACATAAAAAAAAGTCATAGATTTCAATAGAAAGGTTTCTGGCCGACCTAATAAACAAAAAATACGAAACGCAAAAAAAAAAAAAttta                                  >  2:3068325/1‑147 (MQ=255)
                                                                                                                    tcgtTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCt                                <  2:108037/148‑1 (MQ=255)
                                                                                                                    tcgtTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCt                                <  2:2039551/148‑1 (MQ=255)
                                                                                                                    tcgtTTTAGGTGAGTTGTTAATAAAGATTTGGAAATGTGACACAGTGCAAATTCAGACACATAAAAAAACCTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCt                                >  2:3150096/1‑148 (MQ=255)
                                                                                                                     cgtTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTc                               <  1:285847/148‑1 (MQ=255)
                                                                                                                     cgtTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTc                               <  2:747057/148‑1 (MQ=255)
                                                                                                                     cgtTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTc                               <  2:185380/148‑1 (MQ=255)
                                                                                                                        tttAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCAtt                            <  1:1505830/148‑1 (MQ=255)
                                                                                                                        tttAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCAtt                            >  1:3173191/1‑148 (MQ=255)
                                                                                                                        tttAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCAtt                            <  1:920381/148‑1 (MQ=255)
                                                                                                                            ggTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTcc                        >  2:221238/1‑148 (MQ=255)
                                                                                                                               gAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCcaca                     >  1:3416592/1‑148 (MQ=255)
                                                                                                                                aGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCTACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACag                    <  2:611888/148‑1 (MQ=255)
                                                                                                                                   tgttAATAAAGATTTTGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACagtga                 <  1:648237/148‑1 (MQ=255)
                                                                                                                                   tgttAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACagtga                 >  2:1130905/1‑148 (MQ=255)
                                                                                                                                    gttAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACagtgaa                <  1:2326407/148‑1 (MQ=255)
                                                                                                                                       aataaAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACagtgaagtg             <  1:547152/148‑1 (MQ=255)
                                                                                                                                        ataaAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACagtgaagtga            >  1:1305520/1‑148 (MQ=255)
                                                                                                                                         taaAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGAt           >  2:2415121/1‑148 (MQ=255)
                                                                                                                                           aaGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTa         <  2:2879001/148‑1 (MQ=255)
                                                                                                                                            aGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTaa        <  2:2612804/148‑1 (MQ=255)
                                                                                                                                            aGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTaa        >  1:349947/1‑148 (MQ=255)
                                                                                                                                               tttGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTAACTa     >  1:3274652/1‑148 (MQ=255)
                                                                                                                                                  ggAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTAACTATGc  <  2:2778234/148‑1 (MQ=255)
                                                                                                                                                  ggAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTAACTATGc  <  2:1475118/148‑1 (MQ=255)
                                                                                                                                                  ggAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTAACTATGc  <  2:3564832/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTAACTATGC  >  CP009273/3546154‑3546447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑