BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,558,115	C→A	5.9%	A609A (GCG→GCT)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,558,115	0	C	A	5.9%	833.3 / 17.5	219	A609A (GCG→GCT)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base C (103/103); new base A (10/3); total (113/106)
Fisher's exact test for biased strand distribution p-value = 8.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 223 total aligned reads displayed.

GTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTC  >  CP009273/3557969‑3558262

gTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCg                                                                                                                                                    <  2:2041461/148‑1 (MQ=255)

gTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCg                                                                                                                                                    >  2:906809/1‑148 (MQ=255)

 tGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGc                                                                                                                                                   <  2:1856401/148‑1 (MQ=255)

 tGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGc                                                                                                                                                   >  2:1052253/1‑148 (MQ=255)

 tGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTATCGc                                                                                                                                                   >  2:3264935/1‑148 (MQ=255)

   ccGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTa                                                                                                                                                 >  1:1410706/1‑148 (MQ=255)

       aaGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTc                                                                                                                                             >  2:2614773/1‑148 (MQ=255)

       aaGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTc                                                                                                                                             >  1:332082/1‑148 (MQ=255)

       aaGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTc                                                                                                                                             >  1:3521667/1‑148 (MQ=255)

        aGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCNGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCCATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCa                                                                                                                                            <  1:1933191/148‑1 (MQ=255)

           ttCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTg                                                                                                                                         <  1:422295/148‑1 (MQ=255)

           ttCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTg                                                                                                                                         <  1:423834/148‑1 (MQ=255)

            tCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGa                                                                                                                                        >  2:846776/1‑148 (MQ=255)

            tCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGa                                                                                                                                        >  1:2259796/1‑148 (MQ=255)

              cGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATc                                                                                                                                      <  1:2816309/148‑1 (MQ=255)

              cGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATc                                                                                                                                      >  1:2153660/1‑148 (MQ=255)

              cGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATc                                                                                                                                      <  2:367422/148‑1 (MQ=255)

                tCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCaa                                                                                                                                    >  1:1328352/1‑148 (MQ=255)

                    tGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGa                                                                                                                                <  2:2745544/148‑1 (MQ=255)

                    tGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGa                                                                                                                                <  1:1019117/148‑1 (MQ=255)

                     gCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGaa                                                                                                                               <  1:995704/148‑1 (MQ=255)

                          cGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAAtg                                                                                                                          >  2:444255/1‑148 (MQ=255)

                          cGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAAtg                                                                                                                          >  1:2429582/1‑148 (MQ=255)

                          cGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAAtg                                                                                                                          >  2:925177/1‑148 (MQ=255)

                            aaaTCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAAtgtg                                                                                                                        >  2:1951196/1‑148 (MQ=255)

                                    tCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCa                                                                                                                >  1:3647918/1‑148 (MQ=255)

                                        acagaTCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTa                                                                                                            >  2:2924447/1‑148 (MQ=255)

                                         cagaTCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTaa                                                                                                           >  1:939970/1‑148 (MQ=255)

                                          agaTCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAAt                                                                                                          <  1:1352582/148‑1 (MQ=255)

                                          agaTCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGGAGTTCGGGATGAACACGACTTTCAGCTAATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGATTCTCCATGTAAt                                                                                                          >  2:1246964/1‑148 (MQ=255)

                                          agaTCTGCCGCCGGAATGATCAACTGAGCCAGGCTAACGCTTTAGTTCGGGATTAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAAt                                                                                                          <  1:3193500/148‑1 (MQ=255)

                                           gaTCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGTATAATGTGCTTCGCCATGTAATa                                                                                                         <  2:1791181/148‑1 (MQ=255)

                                             tCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAgg                                                                                                       <  2:491814/148‑1 (MQ=255)

                                              cTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGc                                                                                                      >  1:1931203/1‑148 (MQ=255)

                                                   gccgGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAg                                                                                                 <  2:1514438/148‑1 (MQ=255)

                                                   gccgGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAg                                                                                                 <  2:572891/148‑1 (MQ=255)

                                                    ccgGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAgc                                                                                                <  1:2091802/148‑1 (MQ=255)

                                                      ggAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAgccg                                                                                              >  1:399485/1‑148 (MQ=255)

                                                      ggAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAgccg                                                                                              >  1:1225606/1‑148 (MQ=255)

                                                       gAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTCATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAgccgc                                                                                             >  1:1561930/1‑148 (MQ=255)

                                                        aaTGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAgccgcc                                                                                            >  1:3343109/1‑148 (MQ=255)

                                                        aaTGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAgccgcc                                                                                            <  1:2779328/148‑1 (MQ=255)

                                                        aaTGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAgccgcc                                                                                            >  1:1069322/1‑148 (MQ=255)

                                                           gATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTa                                                                                         >  2:3023499/1‑148 (MQ=255)

                                                           gATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTa                                                                                         >  2:1115812/1‑148 (MQ=255)

                                                             tCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTAcc                                                                                       >  1:1944390/1‑148 (MQ=255)

                                                              cAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTACTAGGCCGAAGCCGCCTTACCg                                                                                      >  2:1871413/1‑148 (MQ=255)

                                                              cAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCg                                                                                      >  2:2768870/1‑148 (MQ=255)

                                                                aCTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGcc                                                                                    >  1:3726302/1‑148 (MQ=255)

                                                                 cTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCa                                                                                   >  2:3735421/1‑148 (MQ=255)

                                                                   gcgcCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTAGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCaaa                                                                                 >  2:2050961/1‑148 (MQ=255)

                                                                    cgcCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACAGCCaaaa                                                                                <  1:616324/148‑1 (MQ=255)

                                                                       cAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAata                                                                             <  1:1028223/148‑1 (MQ=255)

                                                                       cAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAata                                                                             <  2:2923994/148‑1 (MQ=255)

                                                                       cAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAata                                                                             <  1:1284305/148‑1 (MQ=255)

                                                                       cAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAata                                                                             <  1:1437039/148‑1 (MQ=255)

                                                                       cAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAata                                                                             <  2:1312828/148‑1 (MQ=255)

                                                                        aGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAatat                                                                            <  2:73624/148‑1 (MQ=255)

                                                                        aGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAatat                                                                            >  1:181400/1‑148 (MQ=255)

                                                                         ggCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATAtt                                                                           <  2:332082/148‑1 (MQ=255)

                                                                         ggCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTAGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATAtt                                                                           >  2:3213327/1‑148 (MQ=255)

                                                                            tAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCAc                                                                        >  1:1748127/1‑148 (MQ=255)

                                                                               cGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCAcgcg                                                                     >  2:94738/1‑148 (MQ=255)

                                                                                     aGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTAc                                                               >  1:2604989/1‑148 (MQ=255)

                                                                                       ttCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTAccc                                                             >  2:863220/1‑148 (MQ=255)

                                                                                       ttCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTAccc                                                             >  1:2203959/1‑148 (MQ=255)

                                                                                        tCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCa                                                            <  1:2362067/148‑1 (MQ=255)

                                                                                           ggATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACtt                                                         <  1:1542246/148‑1 (MQ=255)

                                                                                            gATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTc                                                        >  2:3421021/1‑148 (MQ=255)

                                                                                              tGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGc                                                      <  2:1544479/148‑1 (MQ=255)

                                                                                                       cTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTc                                             >  1:820563/1‑148 (MQ=255)

                                                                                                         ttCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCTGGTCgg                                           >  1:2824286/1‑148 (MQ=255)

                                                                                                         ttCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCgg                                           >  2:2676110/1‑148 (MQ=255)

                                                                                                         ttCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCgg                                           >  2:1642238/1‑148 (MQ=255)

                                                                                                                tATCGCCAATCTGCGGATCGTTGTTGATCACTTTAGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGAt                                    >  2:512023/1‑148 (MQ=255)

                                                                                                                tATCGCCAATCTGCGGATCGTTGTTGATCACTTTAGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGAt                                    >  2:511771/1‑148 (MQ=255)

                                                                                                                 aTCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATg                                   <  2:1931203/148‑1 (MQ=255)

                                                                                                                 aTCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATg                                   <  2:2287/148‑1 (MQ=255)

                                                                                                                     ccAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGt                               >  2:1743983/1‑148 (MQ=255)

                                                                                                                      cAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGtt                              <  2:1328471/148‑1 (MQ=255)

                                                                                                                      cAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGtt                              <  2:3134588/148‑1 (MQ=255)

                                                                                                                          cTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAg                          >  1:334114/1‑148 (MQ=255)

                                                                                                                           tGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGc                         <  2:3521667/148‑1 (MQ=255)

                                                                                                                              ggATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCggg                      <  1:2654188/148‑1 (MQ=255)

                                                                                                                                  cGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAAt                  <  2:3767065/148‑1 (MQ=255)

                                                                                                                                    ttgttgATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATca                >  2:2414711/1‑148 (MQ=255)

                                                                                                                                     tgttgATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATcac               <  2:651064/148‑1 (MQ=255)

                                                                                                                                       ttgATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACAt             >  1:2208553/1‑148 (MQ=255)

                                                                                                                                        tgATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATg            <  2:939185/148‑1 (MQ=255)

                                                                                                                                          aTCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCa          <  1:869048/148‑1 (MQ=255)

                                                                                                                                          aTCACTTTAGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCa          <  2:1704611/148‑1 (MQ=255)

                                                                                                                                           tCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCTTCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCaa         >  2:1100785/1‑148 (MQ=255)

                                                                                                                                           tCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCaa         >  2:2009834/1‑148 (MQ=255)

                                                                                                                                            cACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAac        <  1:3552613/148‑1 (MQ=255)

                                                                                                                                             aCTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAaca       >  1:52389/1‑148 (MQ=255)

                                                                                                                                              cTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAacac      >  1:2717216/1‑148 (MQ=255)

                                                                                                                                              cTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAacac      <  2:3649898/148‑1 (MQ=255)

                                                                                                                                              cTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAacac      >  2:209349/1‑148 (MQ=255)

                                                                                                                                                  cGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATtc  <  1:925177/148‑1 (MQ=255)

                                                                                                                                                  cGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATtc  <  1:444249/148‑1 (MQ=255)

GTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTC  >  CP009273/3557969‑3558262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`