Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,582,807 G→T 100% P194Q (CCG→CAG)  ugpE ← glycerol‑3‑phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,582,8070GT98.7% 769.1 / 0.9 236P194Q (CCG→CAG) ugpEglycerol‑3‑phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (143/90);  total (143/93)
Fisher's exact test for biased strand distribution p-value = 6.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 236 total aligned reads displayed.

TTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGG  >  CP009273/3582662‑3582951
                                                                                                                                                 |                                                                                                                                                
ttCGCCTGGAGCGATCATCCCTTTTATCCCGGCCACGGTGGTGCCGAGACCCCCAGCGGAAAAAAACACCCACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGa                                                                                                                                                <  1:3510186/148‑1 (MQ=255)
    ccTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGaaaaa                                                                                                                                            <  2:1122479/148‑1 (MQ=255)
       gTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGa                                                                                                                                         >  2:731061/1‑148 (MQ=255)
       gTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGa                                                                                                                                         <  2:3570509/148‑1 (MQ=255)
        tAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGAt                                                                                                                                        <  2:3079669/148‑1 (MQ=255)
          gCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATACTCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGt                                                                                                                                      <  1:3780818/148‑1 (MQ=255)
          gCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGt                                                                                                                                      <  1:3635361/148‑1 (MQ=255)
           cGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTc                                                                                                                                     >  2:171546/1‑148 (MQ=255)
             atcatcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGc                                                                                                                                   >  2:1386034/1‑148 (MQ=255)
              tcatcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCa                                                                                                                                  >  1:1975507/1‑148 (MQ=255)
               catcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCaa                                                                                                                                 >  2:171955/1‑148 (MQ=255)
                atcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCaaa                                                                                                                                >  2:429615/1‑148 (MQ=255)
                    cTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaagaa                                                                                                                            <  1:3107709/148‑1 (MQ=255)
                      ttGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAgc                                                                                                                          >  1:2165674/1‑148 (MQ=255)
                      ttGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAgc                                                                                                                          >  2:3192564/1‑148 (MQ=255)
                         aTCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCa                                                                                                                       <  2:771524/148‑1 (MQ=255)
                         aTCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCa                                                                                                                       >  1:3095016/1‑148 (MQ=255)
                            ccTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCATAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTg                                                                                                                    <  1:586544/148‑1 (MQ=255)
                               gCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCg                                                                                                                 <  2:1058759/148‑1 (MQ=255)
                                ccACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGa                                                                                                                <  2:2589680/148‑1 (MQ=255)
                                    ggtggtGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATgcg                                                                                                            <  2:1155597/148‑1 (MQ=255)
                                         tgCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTc                                                                                                       >  1:2147113/1‑148 (MQ=255)
                                             gagaTCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATc                                                                                                   >  1:3352049/1‑148 (MQ=255)
                                              agaTCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATcc                                                                                                  >  1:1779450/1‑148 (MQ=255)
                                               gaTCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcg                                                                                                 >  2:3233774/1‑148 (MQ=255)
                                                aTCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgc                                                                                                >  2:1934774/1‑148 (MQ=255)
                                                 tCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcg                                                                                               <  1:3730122/148‑1 (MQ=255)
                                                  ccACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATCAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcgc                                                                                              >  1:716994/1‑148 (MQ=255)
                                                    acaTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCgg                                                                                            <  1:923569/148‑1 (MQ=255)
                                                     caTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGc                                                                                           >  1:76429/1‑148 (MQ=255)
                                                     caTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGc                                                                                           >  1:1006691/1‑148 (MQ=255)
                                                      aTCTGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCt                                                                                          >  2:2968092/1‑148 (MQ=255)
                                                      aTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCt                                                                                          >  1:1235072/1‑148 (MQ=255)
                                                       tCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCtt                                                                                         >  1:1486228/1‑148 (MQ=255)
                                                       tCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCtt                                                                                         >  2:2878531/1‑148 (MQ=255)
                                                       tCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCtt                                                                                         >  1:3806713/1‑148 (MQ=255)
                                                       tCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCtt                                                                                         >  1:2619762/1‑148 (MQ=255)
                                                        cGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTc                                                                                        <  1:3406305/148‑1 (MQ=255)
                                                         ggTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTcc                                                                                       >  1:2595874/1‑148 (MQ=255)
                                                          gTAATAATCAACAACGGCAACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGAATAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTcca                                                                                      >  2:2734192/1‑148 (MQ=255)
                                                            aataatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTccacc                                                                                    >  1:388903/1‑148 (MQ=255)
                                                             ataatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTccacca                                                                                   >  2:2555018/1‑148 (MQ=255)
                                                              taatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAg                                                                                  >  2:1022408/1‑148 (MQ=255)
                                                              taatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAg                                                                                  >  2:3432330/1‑148 (MQ=255)
                                                               aatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGc                                                                                 >  2:827745/1‑148 (MQ=255)
                                                               aatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGc                                                                                 >  1:2278831/1‑148 (MQ=255)
                                                                atCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCt                                                                                >  1:3791385/1‑148 (MQ=255)
                                                                 tCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTc                                                                               >  2:1066076/1‑148 (MQ=255)
                                                                  caacaaCGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCa                                                                              >  2:646643/1‑148 (MQ=255)
                                                                   aacaacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCAt                                                                             <  1:1213637/148‑1 (MQ=255)
                                                                   aacaacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCAt                                                                             <  1:1716090/148‑1 (MQ=255)
                                                                        cGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGc                                                                        >  2:1136619/1‑148 (MQ=255)
                                                                             acaAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGt                                                                   <  2:356551/148‑1 (MQ=255)
                                                                               aaaTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCa                                                                 >  1:134162/1‑148 (MQ=255)
                                                                                  tACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaa                                                              >  1:894537/1‑148 (MQ=255)
                                                                                   aCTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaaa                                                             >  1:2045423/1‑148 (MQ=255)
                                                                                   aCTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaaa                                                             >  2:1323796/1‑148 (MQ=255)
                                                                                      gATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGaa                                                          <  1:711121/148‑1 (MQ=255)
                                                                                      gATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGaa                                                          >  1:2416974/1‑148 (MQ=255)
                                                                                      gATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGaa                                                          <  2:1389927/148‑1 (MQ=255)
                                                                                       aTTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAAc                                                         >  1:345330/1‑148 (MQ=255)
                                                                                         tCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTg                                                       <  2:1070268/148‑1 (MQ=255)
                                                                                         tCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTg                                                       >  1:1166576/1‑148 (MQ=255)
                                                                                         tCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTg                                                       <  2:1862921/148‑1 (MQ=255)
                                                                                             gCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCgg                                                   >  1:771356/1‑148 (MQ=255)
                                                                                               cGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGaa                                                 <  2:2640596/148‑1 (MQ=255)
                                                                                                gTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAAc                                                >  2:2186729/1‑148 (MQ=255)
                                                                                                 tAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACa                                               <  2:3422939/148‑1 (MQ=255)
                                                                                                  aGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAg                                              >  2:2500538/1‑148 (MQ=255)
                                                                                                   gATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAgg                                             <  1:97391/148‑1 (MQ=255)
                                                                                                    aTAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGa                                            >  1:3042569/1‑148 (MQ=255)
                                                                                                      aaaGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAATGAACTGGCGGAACAGGaaa                                          >  2:1360354/1‑148 (MQ=255)
                                                                                                        aGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGt                                        <  1:3145701/148‑1 (MQ=255)
                                                                                                          gTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAg                                      <  2:2377201/148‑1 (MQ=255)
                                                                                                          gTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAg                                      <  1:445542/148‑1 (MQ=255)
                                                                                                            gATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCg                                    >  2:761937/1‑148 (MQ=255)
                                                                                                            gATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCg                                    <  2:3352049/148‑1 (MQ=255)
                                                                                                             aTCACAAACCGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCgg                                   <  2:224552/148‑1 (MQ=255)
                                                                                                               cacaAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTc                                 >  2:2586563/1‑148 (MQ=255)
                                                                                                               cacaAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGAGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTc                                 >  2:1965456/1‑148 (MQ=255)
                                                                                                                 caAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGc                               >  1:126882/1‑148 (MQ=255)
                                                                                                                 caAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGc                               >  1:2757553/1‑148 (MQ=255)
                                                                                                                   aaCAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCg                             <  2:2331764/148‑1 (MQ=255)
                                                                                                                        cgccgcCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGcc                        >  2:1277599/1‑148 (MQ=255)
                                                                                                                        cgccgcCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGcc                        >  2:1464687/1‑148 (MQ=255)
                                                                                                                            gccAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCa                    >  1:3648298/1‑148 (MQ=255)
                                                                                                                                gATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCgg                >  2:678312/1‑148 (MQ=255)
                                                                                                                                 atgaGTTTTGGAGAGCTGAAAAACGATGTCGCAACAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGc               <  2:2077341/145‑1 (MQ=255)
                                                                                                                                 aTTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGc               >  1:1088252/1‑148 (MQ=255)
                                                                                                                                 aTTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGc               >  1:3278768/1‑148 (MQ=255)
                                                                                                                                     gTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCCCCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCg           <  1:3373187/148‑1 (MQ=255)
                                                                                                                                      ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          >  2:2563630/1‑148 (MQ=255)
                                                                                                                                      ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          >  1:1890068/1‑148 (MQ=255)
                                                                                                                                      ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          >  2:948815/1‑148 (MQ=255)
                                                                                                                                       tttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGtt         >  1:2730912/1‑148 (MQ=255)
                                                                                                                                       tttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGtt         >  1:2678107/1‑148 (MQ=255)
                                                                                                                                         tGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTaa       <  1:1254730/148‑1 (MQ=255)
                                                                                                                                          ggAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTaaa      <  2:1029359/148‑1 (MQ=255)
                                                                                                                                           gagaGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAAc     >  1:2945988/1‑148 (MQ=255)
                                                                                                                                              agCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCgg  <  1:791875/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGG  >  CP009273/3582662‑3582951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑