Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 5,144 G→A 100% intergenic (+124/‑90) thrC → / → yaaX L‑threonine synthase/DUF2502 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092735,1440GA99.5% 585.5 / ‑4.4 181intergenic (+124/‑90)thrC/yaaXL‑threonine synthase/DUF2502 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (95/85);  total (95/86)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

ATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCAT  >  CP009273/4999‑5289
                                                                                                                                                 |                                                                                                                                                 
aTTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAAtt                                                                                                                                                <  2:961933/149‑1 (MQ=255)
  tgatgatgaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAg                                                                                                                                              <  1:3811925/149‑1 (MQ=255)
   gatgatgaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAgg                                                                                                                                             >  2:3716521/1‑149 (MQ=255)
    atgatgaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGa                                                                                                                                            <  1:1223989/149‑1 (MQ=255)
    atgatgaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGa                                                                                                                                            <  1:284349/149‑1 (MQ=255)
          aaTCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCgg                                                                                                                                      >  2:3902337/1‑149 (MQ=255)
          aaTCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCgg                                                                                                                                      >  1:2117269/1‑149 (MQ=255)
          aaTCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCgg                                                                                                                                      <  1:1909739/149‑1 (MQ=255)
           atcatcAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGga                                                                                                                                     >  2:2595510/1‑149 (MQ=255)
           atcatcAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGga                                                                                                                                     >  2:3579351/1‑149 (MQ=255)
              atcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGaa                                                                                                                                  >  2:1846888/1‑149 (MQ=255)
              atcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGaa                                                                                                                                  >  2:3520151/1‑149 (MQ=255)
               tcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAAt                                                                                                                                 <  2:2728989/149‑1 (MQ=255)
                    aaaaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATaacaa                                                                                                                            >  1:3269666/1‑149 (MQ=255)
                    aaaaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATaacaa                                                                                                                            <  1:1500495/149‑1 (MQ=255)
                     aaaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATaacaac                                                                                                                           <  2:1019025/149‑1 (MQ=255)
                      aaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAAcc                                                                                                                          >  1:1750785/1‑149 (MQ=255)
                        tCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAAccgc                                                                                                                        >  2:1229309/1‑149 (MQ=255)
                         cTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAAccgcc                                                                                                                       <  1:2221688/149‑1 (MQ=255)
                            ttCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGtt                                                                                                                    >  1:3393113/1‑149 (MQ=255)
                             tCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACTGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTtc                                                                                                                   <  2:3411291/149‑1 (MQ=255)
                             tCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTtc                                                                                                                   <  2:2390042/149‑1 (MQ=255)
                              cATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTtct                                                                                                                  >  1:704622/1‑149 (MQ=255)
                               aTTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTtctc                                                                                                                 <  1:1896642/149‑1 (MQ=255)
                                 tATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCAt                                                                                                               <  2:2810740/149‑1 (MQ=255)
                                  aTCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTACTCATc                                                                                                              >  2:2449562/1‑149 (MQ=255)
                                   tctcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCg                                                                                                             >  1:2504703/1‑149 (MQ=255)
                                   tctcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCg                                                                                                             <  1:3607675/149‑1 (MQ=255)
                                    ctcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATTGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATcta                                                                                                            >  1:1452637/1‑147 (MQ=255)
                                    ctcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGa                                                                                                            <  1:906014/149‑1 (MQ=255)
                                     tcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAg                                                                                                           <  1:3246249/149‑1 (MQ=255)
                                     tcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAg                                                                                                           <  2:4059545/149‑1 (MQ=255)
                                      cAATCAGGCGGGGTTTTCTTTTATGCAGCCCGGATTTTTTATGAAGAATTTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTTGGATTGCGGTTATTAACAACCGCCGTTCTCATCGAGt                                                                                                          <  1:560443/149‑1 (MQ=255)
                                        aTCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTaa                                                                                                        >  1:1071004/1‑149 (MQ=255)
                                           aGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAAtct                                                                                                     <  2:1634413/149‑1 (MQ=255)
                                            ggCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAAtctc                                                                                                    >  2:681740/1‑149 (MQ=255)
                                             gCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTcc                                                                                                   >  1:1959485/1‑149 (MQ=255)
                                              ccGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCg                                                                                                  <  2:1495487/149‑1 (MQ=255)
                                              ccGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCg                                                                                                  >  1:3996418/1‑149 (MQ=255)
                                                 ggTTTGCTTTTCTGCAGCCCGGCTTTTTTATGAATAAATTAGGTAGAAAAATGACAGGGAAATAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACCACCGCCGTTCTCATCGAGTAATCTCCGGat                                                                                               <  1:2885532/149‑1 (MQ=255)
                                                  gTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGata                                                                                              >  2:1308410/1‑149 (MQ=255)
                                                  gTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGata                                                                                              >  2:3777308/1‑149 (MQ=255)
                                                   tttGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGatat                                                                                             >  1:3347979/1‑149 (MQ=255)
                                                   tttGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGatat                                                                                             >  2:2339997/1‑149 (MQ=255)
                                                     tGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCg                                                                                           >  2:2631555/1‑149 (MQ=255)
                                                      gCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGa                                                                                          >  2:961404/1‑149 (MQ=255)
                                                              gCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAAc                                                                                  <  2:1532116/149‑1 (MQ=255)
                                                              gCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAAc                                                                                  <  1:2104103/149‑1 (MQ=255)
                                                                aGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACgg                                                                                >  2:1618982/1‑149 (MQ=255)
                                                                aGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACgg                                                                                <  1:256312/149‑1 (MQ=255)
                                                                   ccGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCa                                                                             >  1:651945/1‑149 (MQ=255)
                                                                      gCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATg                                                                          >  1:1470016/1‑149 (MQ=255)
                                                                       cTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGa                                                                         <  2:785168/149‑1 (MQ=255)
                                                                         tttttATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTTATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATa                                                                       <  2:1734965/149‑1 (MQ=255)
                                                                          ttttATTAAGAAATTATGGAGAAAAATGCCAGGGAAAAAGGAGATATTCTCAATATATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaa                                                                      <  2:2839746/149‑1 (MQ=255)
                                                                            ttATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaaa                                                                    <  2:2260789/149‑1 (MQ=255)
                                                                              aTGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAgg                                                                  >  2:691596/1‑149 (MQ=255)
                                                                               tGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGa                                                                 <  2:1207348/149‑1 (MQ=255)
                                                                                 aagaaATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGt                                                               >  2:1049263/1‑149 (MQ=255)
                                                                                 aagaaATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGt                                                               <  1:1313253/149‑1 (MQ=255)
                                                                                   gaaATTATGTAGAAAAATGACATTGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGTTTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTaa                                                             <  2:1801732/149‑1 (MQ=255)
                                                                                   gaaATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTaa                                                             >  2:2468286/1‑149 (MQ=255)
                                                                                   gaaATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTaa                                                             <  2:2092737/149‑1 (MQ=255)
                                                                                   gaaATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTaa                                                             >  1:1417547/1‑149 (MQ=255)
                                                                                     aaTTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAAcc                                                           <  1:3166061/149‑1 (MQ=255)
                                                                                     aaTTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAAcc                                                           <  2:473801/149‑1 (MQ=255)
                                                                                      aTTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCt                                                          <  2:520114/149‑1 (MQ=255)
                                                                                        tATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgt                                                        <  2:2609511/149‑1 (MQ=255)
                                                                                           ggAGAAAAATGACAGTGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaa                                                     <  2:2452758/149‑1 (MQ=255)
                                                                                            gagaAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaaa                                                    >  1:1657214/1‑149 (MQ=255)
                                                                                              gaAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaaaaa                                                  <  2:3068147/149‑1 (MQ=255)
                                                                                                aaaaTGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGa                                                >  2:3985891/1‑149 (MQ=255)
                                                                                                aaaaTGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGa                                                >  2:3209310/1‑149 (MQ=255)
                                                                                                 aaaTGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGAt                                               <  1:1522785/149‑1 (MQ=255)
                                                                                                 aaaTGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGAt                                               <  2:2777875/149‑1 (MQ=255)
                                                                                                 aaaTGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGAt                                               <  2:2785928/149‑1 (MQ=255)
                                                                                                   aTGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGc                                             <  1:1229309/149‑1 (MQ=255)
                                                                                                     gACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCaa                                           <  1:3184039/149‑1 (MQ=255)
                                                                                                         gggAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTa                                       >  2:1467034/1‑149 (MQ=255)
                                                                                                         gggAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTa                                       <  2:2583618/149‑1 (MQ=255)
                                                                                                         gggAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTa                                       >  1:3515732/1‑149 (MQ=255)
                                                                                                         gggAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTa                                       >  1:3515768/1‑149 (MQ=255)
                                                                                                            aaaaaGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCg                                    >  2:1941775/1‑149 (MQ=255)
                                                                                                             aaaaGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACTGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGt                                   >  1:3174592/1‑149 (MQ=255)
                                                                                                             aaaaGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGt                                   >  1:3489408/1‑149 (MQ=255)
                                                                                                             aaaaGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGt                                   >  2:3233785/1‑149 (MQ=255)
                                                                                                               aaGGAGAAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTAc                                 >  1:3511029/1‑149 (MQ=255)
                                                                                                                  gagaAATCCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCg                              >  1:238425/1‑149 (MQ=255)
                                                                                                                   agaAATTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGc                             >  2:3325173/1‑149 (MQ=255)
                                                                                                                     aaaTTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCAc                           <  1:3438570/149‑1 (MQ=255)
                                                                                                                       aTTCTCAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACtt                         >  1:121213/1‑149 (MQ=255)
                                                                                                                           tcAATAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTccc                     <  2:1545249/149‑1 (MQ=255)
                                                                                                                               tAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGt                 <  1:3842711/149‑1 (MQ=255)
                                                                                                                               tAAATGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGt                 >  1:51855/1‑149 (MQ=255)
                                                                                                                                aaaTGCGGTAAATTAGAAATTTGGATTGATGAGAATAACAACCGCCGTTCTGATCGAGTAATCTCCGGATATCGACCCATAACGGGCATTGATAAATGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGtt                <  2:3801469/149‑1 (MQ=255)
                                                                                                                                  aTGCGGTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCt              >  1:3444294/1‑149 (MQ=255)
                                                                                                                                      ggTAACTTAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTc          <  1:3373937/149‑1 (MQ=255)
                                                                                                                                            ttAGAAATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTccc    >  2:1336103/1‑149 (MQ=255)
                                                                                                                                              agaaATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCAt  <  1:2298552/149‑1 (MQ=255)
                                                                                                                                              agaaATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCAt  <  1:2298834/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCAT  >  CP009273/4999‑5289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑