Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 31,710 C→A 100% I298I (ATC→ATA)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927331,7100CA97.4% 593.4 / 2.6 200I298I (ATC→ATA) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (1/6);  new base A (122/70);  total (123/77)
Fisher's exact test for biased strand distribution p-value = 1.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 201 total aligned reads displayed.

GTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCT  >  CP009273/31564‑31855
                                                                                                                                                  |                                                                                                                                                 
gTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGa                                                                                                                                                 >  1:1639439/1‑149 (MQ=255)
    cgcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATg                                                                                                                                             <  1:605895/149‑1 (MQ=255)
     gcCAGCCCAACCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGa                                                                                                                                            <  2:3605379/149‑1 (MQ=255)
      ccAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGaa                                                                                                                                           <  2:837515/149‑1 (MQ=255)
      ccAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGaa                                                                                                                                           >  1:2169170/1‑149 (MQ=255)
      ccAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGaa                                                                                                                                           >  1:2832519/1‑149 (MQ=255)
        aGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAcc                                                                                                                                         <  1:3828396/149‑1 (MQ=255)
        aGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAcc                                                                                                                                         <  1:1816239/149‑1 (MQ=255)
            cAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcg                                                                                                                                     >  1:2919536/1‑149 (MQ=255)
            cAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcg                                                                                                                                     >  2:2264285/1‑149 (MQ=255)
              aaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcgcg                                                                                                                                   >  1:2159173/1‑149 (MQ=255)
                cGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCgtg                                                                                                                                 <  1:2714109/149‑1 (MQ=255)
                 gCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCgtgt                                                                                                                                >  1:3532125/1‑149 (MQ=255)
                  cTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTc                                                                                                                               <  2:2445506/149‑1 (MQ=255)
                    gACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTccc                                                                                                                             >  1:1396663/1‑149 (MQ=255)
                       cGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGtt                                                                                                                          <  1:96329/149‑1 (MQ=255)
                           aaaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                      >  2:2351062/1‑149 (MQ=255)
                           aaaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                      <  2:3348237/149‑1 (MQ=255)
                            aaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                     >  1:2474463/1‑149 (MQ=255)
                            aaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                     >  1:4025961/1‑149 (MQ=255)
                                 tatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCt                                                                                                                <  1:105555/149‑1 (MQ=255)
                                     aaaTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCg                                                                                                            <  1:2278985/149‑1 (MQ=255)
                                      aaTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGt                                                                                                           >  1:3289733/1‑149 (MQ=255)
                                         cATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGa                                                                                                        >  1:27270/1‑149 (MQ=255)
                                            gCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAg                                                                                                     <  1:279135/149‑1 (MQ=255)
                                              gTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                   >  1:367218/1‑149 (MQ=255)
                                               tAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                  >  1:1107887/1‑149 (MQ=255)
                                               tAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                  >  2:1330957/1‑149 (MQ=255)
                                               tAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                  <  1:2351062/149‑1 (MQ=255)
                                                aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAc                                                                                                 >  1:3131365/1‑149 (MQ=255)
                                                 aCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                                >  2:2908965/1‑149 (MQ=255)
                                                 aCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                                >  1:3357163/1‑149 (MQ=255)
                                                 aCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                                >  2:1262258/1‑149 (MQ=255)
                                                   gCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCgg                                                                                              >  1:26263/1‑149 (MQ=255)
                                                   gCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCgg                                                                                              >  2:595491/1‑149 (MQ=255)
                                                      tCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGttt                                                                                           >  2:2192213/1‑149 (MQ=255)
                                                      tCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGttt                                                                                           >  2:1929519/1‑149 (MQ=255)
                                                      tCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGttt                                                                                           >  1:492387/1‑149 (MQ=255)
                                                       cGATGGCGGTGCTGCGTGACATCGGCGTGTAACCCGGTGGTGCAACCGTTCTGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGACCCCCCGCGGGGCCCGTTGTTCGGCGCGGGGGTCGAAAGCGACCGGTTTc                                                                                          <  2:2964457/149‑1 (MQ=255)
                                                        gATGGCGGTGCTGCGTGAAATCGGCGTTGAAACAGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAACCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTcc                                                                                         >  1:2386162/1‑149 (MQ=255)
                                                         aTGGCGGTGCTGCGTGAAATCGGCGTTGAAAACGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc                                                                                        >  2:1768600/1‑149 (MQ=255)
                                                          gggCGGTGGTGCTTGAAATCGGGGTTTAAACGGGTGGTTCAAACTTTCATTTTGGGGTTAACCCTAAAAAGGTTCGTCTTATTTTTATATAATTTAACCCACGCGGGTCCCGTTCTTCGGCGCTGGCGTCCAAAGCGACCGGTTTCCCg                                                                                       <  2:821221/148‑1 (MQ=255)
                                                            gCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGACCCCACGCGGGTCCCGTTCTTCGGGGCTGGCTTCGAAAGCGACCGGTTTCCCGAt                                                                                     <  2:3737120/149‑1 (MQ=255)
                                                                tgctgcGTGATAGGGGCGTTTAAACGGGTGGTTCCAAGGTTCTGTTTGCGGTGAACCCTAAAACGGGTCGTCTGTTTTTTTTAGAAATTACCCCCCGCGGGGCCCGGTCGTGGGCGCGGGCGTCGAACGCGACCGGTTTCCCGATTGCt                                                                                 <  2:3501425/149‑1 (MQ=255)
                                                                    gcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAg                                                                             >  1:1842763/1‑149 (MQ=255)
                                                                      gTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTg                                                                           >  2:2548723/1‑149 (MQ=255)
                                                                        gAAATCGGCGTTGAAACCGGTGTTTCCAACGTTATGTTTGCGTTGAACCCGAAAAACGGTCGTCTGTTTTTTATATAAATGAACCCCCGCGGGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggc                                                                         <  2:3369581/149‑1 (MQ=255)
                                                                        gAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggc                                                                         >  2:2615763/1‑149 (MQ=255)
                                                                          aaTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTtgcgg                                                                       >  1:1833089/1‑149 (MQ=255)
                                                                            tCGGCGTTGAAACCGGTGGTTCCAAAGTTCAGTTTTCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATATAAATTAACCCACGCGTGTCCCGTTCTTCGGCGCGGGCGTCGAAAGCGAACGGTTTACCGATTGCTAAATTGgcggcg                                                                     >  1:3197652/1‑149 (MQ=255)
                                                                               gCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaaa                                                                  >  1:1804232/1‑149 (MQ=255)
                                                                               gCGATGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaca                                                                  >  2:826689/1‑147 (MQ=255)
                                                                                 gTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGGTTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACt                                                                >  1:3801441/1‑149 (MQ=255)
                                                                                 gTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACt                                                                >  2:3231590/1‑149 (MQ=255)
                                                                                  ttGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTg                                                               >  2:3098488/1‑149 (MQ=255)
                                                                                   tGAACCGGGTGGTTCCAAGTTACTGTGGGGGGTGAACCCGAAAACGGGTGGTCTGATTGTTATATAAATGACCCCCCGGGTGCCCCGTTCTTGGGCGGTGGCGGCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTgg                                                              <  1:1337317/149‑1 (MQ=255)
                                                                                      aaCCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCgg                                                           >  2:2147830/1‑149 (MQ=255)
                                                                                       aCCGGTGTTTCAAACGTTCTTTTTGCGTTTAACCCCAAAAACGGTCTTCTTTTTTTTATATAAATCAACCCACGCGTGCCCCGTTATTCGGCGCTGGCGTCCAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGt                                                          <  2:1618837/149‑1 (MQ=255)
                                                                                       aCCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGt                                                          >  1:4036165/1‑149 (MQ=255)
                                                                                          ggtggtTCAAAGGTTCTGTTTGCGGGCAACCCTAAAACCGGTCGTCTGTTTGTTATCGAAAGCAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCCAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTggg                                                       <  1:3762653/149‑1 (MQ=255)
                                                                                          gggggtACCACCGTTCAGTTTGCGGTGCACCCGAAAAACGGTCGTCGGATTGTTATAGAATTGACCCCACGGGTGGCCGGTTATTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTggg                                                       <  2:3549927/146‑1 (MQ=255)
                                                                                           gtggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGt                                                      >  1:299824/1‑149 (MQ=255)
                                                                                           gtggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCGAAAGTGGCGGCGACACTGGCGGTGGGt                                                      >  1:2172734/1‑149 (MQ=255)
                                                                                            tggcTCCAACGTCCAGTTGGCGGTGAACCCGAAAACCGGTCGTCTGATGGTTATCGACTCGACCCCCGGCGGGTCCCGTTGTTCGGCGCGGGGGTCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGtt                                                     <  2:2414934/149‑1 (MQ=255)
                                                                                             ggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTa                                                    >  1:2351583/1‑149 (MQ=255)
                                                                                             ggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTa                                                    >  1:3838680/1‑149 (MQ=255)
                                                                                                  cAACGTTCTTTTTGCGGTGAACCCGAAAACCGGTCGTCTGATTGTTATACAAATGACCCCACGCGTGCCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAccc                                               <  2:3473486/149‑1 (MQ=255)
                                                                                                           gTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc                                      >  2:440934/1‑149 (MQ=255)
                                                                                                           gTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc                                      >  2:440983/1‑149 (MQ=255)
                                                                                                           gTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc                                      >  1:650194/1‑149 (MQ=255)
                                                                                                             ttGCGGTGAACCCGAAAAAGGGTGGTCTGATTTTTATAGAAATCACCCCCCGCGGGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtg                                    <  2:905473/149‑1 (MQ=255)
                                                                                                             ttGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtg                                    >  1:2877042/1‑149 (MQ=255)
                                                                                                             ttGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtg                                    >  1:815676/1‑149 (MQ=255)
                                                                                                                 ggTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtgatga                                >  2:1970734/1‑149 (MQ=255)
                                                                                                                  ttGAACCCTAAAAACGTTCTTCTTTTTTTTATAGAAATGAACCCACGGGGGGCCGGTTCTTGGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGaa                               <  2:3532125/148‑1 (MQ=255)
                                                                                                                  gTGACCCCCAAACACGGTCTTCTGTTTTTTATAGAAATGAACCCCCGGGTGGCGGGTTGTGGGCGGCTGGCGTCTAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGaa                               <  2:2787493/149‑1 (MQ=255)
                                                                                                                  gTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGaa                               >  1:1980849/1‑149 (MQ=255)
                                                                                                                  gTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGaa                               >  1:1979898/1‑149 (MQ=255)
                                                                                                                   tGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAAc                              >  1:1267529/1‑149 (MQ=255)
                                                                                                                    gAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACg                             >  1:3467917/1‑149 (MQ=255)
                                                                                                                     acccccAAAAAGGGTCTTCTTTTTTTTATAGAAATGAACCCACGGGTGTCCCGTTCTTCGGGGCGGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGa                            <  2:3824771/147‑1 (MQ=255)
                                                                                                                     aaCCCGAAAAACGGTCGTCTGATTGTTATAGAAATGACCCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGa                            <  2:3648938/149‑1 (MQ=255)
                                                                                                                        ccGAAAAACGGTCGTCTGATTGTTATAGAAATTAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACAGATGAACGACAt                         >  1:205964/1‑149 (MQ=255)
                                                                                                                         ctaaaaaGGGTCGTCTTATTTTTAAAGAAATCAACCCCCGGGGGGCCGGTTCTTGGGCGCGGGCTCCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATc                        <  1:1921427/147‑1 (MQ=255)
                                                                                                                          gAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCa                       <  2:4026040/149‑1 (MQ=255)
                                                                                                                            aaacgggtggtCTGTTTGTTATTTAATTGAACCCACGGGTGTCCCGTTCTTGGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTACAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACt                     <  2:3798398/144‑1 (MQ=255)
                                                                                                                                  gtcttcTGTTTGTTATACAAATCAACCCCGGCGGGCCCGGTTCTCGGGCGCGGGGGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGa               <  1:1330957/149‑1 (MQ=255)
                                                                                                                                   tcgtcTTATTGTTATAGAAATGAACCCACGCGGGTCCGGTTCGTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGAc              <  2:2357218/149‑1 (MQ=255)
                                                                                                                                    cgtgttaTTTTTATCTAAATGAACCCACGGGTGTCCCGTTCTTCGGCGCGGGCGTCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACg             <  2:1475829/143‑1 (MQ=255)
                                                                                                                                      tcTGATTGTTATAGAACTGACCCCCCGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTa           <  2:3429687/149‑1 (MQ=255)
                                                                                                                                      tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTa           >  1:1510580/1‑149 (MQ=255)
                                                                                                                                      tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTa           >  1:1720083/1‑149 (MQ=255)
                                                                                                                                      tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTa           >  1:3573565/1‑149 (MQ=255)
                                                                                                                                        tGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGGACGACATCACTGGCGGACGTACt         >  2:1134629/1‑149 (MQ=255)
                                                                                                                                          aTTGTTATAGAACTGAACCCACGCGTGGCCCGTACTTCGGCGCTGGCGTCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTcc       <  2:2247070/149‑1 (MQ=255)
                                                                                                                                           ttGTTATATAAATGACCCCCCGCGGGTCCCGTTCTTGGGCGGGGGCGTCCAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCg      <  2:367218/149‑1 (MQ=255)
                                                                                                                                             gTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGc    >  2:905133/1‑149 (MQ=255)
                                                                                                                                             gTTATACAAATTAACCCACGGGGGGCCCGTTCTGGGGCGGGGGCGTCTAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGc    <  1:1685387/149‑1 (MQ=255)
                                                                                                                                              ttATAGAAATGAACCCCCGCGGGGCCGGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcc   <  1:3367528/149‑1 (MQ=255)
                                                                                                                                              ttATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcc   >  2:1071383/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCT  >  CP009273/31564‑31855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑