BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,557,688	C→A	32.1%	D752Y (GAT→TAT)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,557,688	0	C	A	32.1%	472.7 / 217.6	268	D752Y (GAT→TAT)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base C (102/80); new base A (46/40); total (148/120)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 268 total aligned reads displayed.

ATAGTACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGC  >  CP009273/3557543‑3557833

aTAGTACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGcc                                                                                                                                                >  1:1386180/1‑149 (MQ=255)

  aGTACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGa                                                                                                                                              >  2:579881/1‑149 (MQ=255)

  aGTACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGa                                                                                                                                              <  2:1605478/149‑1 (MQ=255)

  aGTACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGa                                                                                                                                              <  2:1340240/149‑1 (MQ=255)

   gTACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGaa                                                                                                                                             >  2:1508051/1‑149 (MQ=255)

     aCGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGt                                                                                                                                           <  1:2715143/149‑1 (MQ=255)

     aCGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGt                                                                                                                                           <  1:2553550/149‑1 (MQ=255)

      cGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGtt                                                                                                                                          <  1:1583800/149‑1 (MQ=255)

       ggTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCTCCGAAGATg                                                                                                                                         >  1:736836/1‑149 (MQ=255)

        gTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGa                                                                                                                                        <  2:4080942/149‑1 (MQ=255)

           agaagagaagTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCa                                                                                                                                     >  2:3238605/1‑149 (MQ=255)

            gaagagaagTAGCCCATATTGGGAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGCAGTTGATAAg                                                                                                                                    >  2:2817090/1‑149 (MQ=255)

              agagaagTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAgag                                                                                                                                  >  2:3435845/1‑147 (MQ=255)

              agagaagTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCg                                                                                                                                  >  2:3449813/1‑149 (MQ=255)

               gagaagTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGa                                                                                                                                 >  1:93078/1‑149 (MQ=255)

               gagaagTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGa                                                                                                                                 >  1:2814743/1‑149 (MQ=255)

                agaagTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGaa                                                                                                                                >  1:667297/1‑149 (MQ=255)

                agaagTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGaa                                                                                                                                >  1:303285/1‑149 (MQ=255)

                   agTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCa                                                                                                                             >  1:209832/1‑149 (MQ=255)

                      aGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAAcc                                                                                                                          >  1:3689647/1‑149 (MQ=255)

                       gCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCa                                                                                                                         >  1:497211/1‑149 (MQ=255)

                       gCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTGCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCa                                                                                                                         <  1:3451020/149‑1 (MQ=255)

                         ccATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGa                                                                                                                       <  2:1351250/149‑1 (MQ=255)

                           atatTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATc                                                                                                                     >  2:194612/1‑149 (MQ=255)

                                ggCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGAt                                                                                                                <  1:1004180/149‑1 (MQ=255)

                                ggCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGAt                                                                                                                <  1:3057768/149‑1 (MQ=255)

                                ggCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGAt                                                                                                                <  1:132682/149‑1 (MQ=255)

                                  cAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAg                                                                                                              >  2:4102804/1‑149 (MQ=255)

                                   aaTGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGc                                                                                                             >  2:2949289/1‑149 (MQ=255)

                                     tGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGa                                                                                                           <  1:2169361/149‑1 (MQ=255)

                                     tGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGa                                                                                                           <  1:941613/149‑1 (MQ=255)

                                     tGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGa                                                                                                           <  1:2998904/149‑1 (MQ=255)

                                       ttCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGAcc                                                                                                         >  1:759656/1‑149 (MQ=255)

                                          aGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCgg                                                                                                      >  2:2689772/1‑149 (MQ=255)

                                             ttCGCTTTTGCGGTCCACTCTTCCTTAAGATAGTAGAGTTAATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGTTAGTGATCGCCGAATTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATc                                                                                                   <  2:1906709/148‑1 (MQ=255)

                                             aTCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCAGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATc                                                                                                   >  2:2170160/1‑149 (MQ=255)

                                              tCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCt                                                                                                  >  1:3986859/1‑149 (MQ=255)

                                              tCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCt                                                                                                  <  2:1456476/149‑1 (MQ=255)

                                              tCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCt                                                                                                  <  2:3502293/149‑1 (MQ=255)

                                                gCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTc                                                                                                >  1:3994752/1‑149 (MQ=255)

                                                gCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTc                                                                                                <  2:2373818/149‑1 (MQ=255)

                                                         gTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGaa                                                                                       <  2:3727639/149‑1 (MQ=255)

                                                          tCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAAt                                                                                      >  1:345464/1‑149 (MQ=255)

                                                            cACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATac                                                                                    >  1:3542494/1‑149 (MQ=255)

                                                             aCTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATaca                                                                                   >  1:2375464/1‑149 (MQ=255)

                                                              ctctTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATacac                                                                                  <  2:602030/149‑1 (MQ=255)

                                                              ctctTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATacac                                                                                  >  1:1255003/1‑149 (MQ=255)

                                                                 ttCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGc                                                                               >  2:3790476/1‑149 (MQ=255)

                                                                      gAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCg                                                                          >  1:3608073/1‑149 (MQ=255)

                                                                        aGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGAt                                                                        <  2:2814743/149‑1 (MQ=255)

                                                                        aGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGAt                                                                        <  2:909390/149‑1 (MQ=255)

                                                                        aGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGAt                                                                        <  2:751869/149‑1 (MQ=255)

                                                                         gCTCGTATATTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCTGTACCGGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCTATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGAtt                                                                       <  1:2500015/149‑1 (MQ=255)

                                                                         gCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGAtt                                                                       <  2:3182726/149‑1 (MQ=255)

                                                                         gCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGAtt                                                                       >  2:2861005/1‑149 (MQ=255)

                                                                         gCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCTAtt                                                                       >  1:824408/1‑149 (MQ=255)

                                                                          cTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGAttt                                                                      >  1:1799530/1‑149 (MQ=255)

                                                                          cTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGAttt                                                                      <  2:1859575/149‑1 (MQ=255)

                                                                            cGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGc                                                                    <  2:449605/149‑1 (MQ=255)

                                                                            cGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGc                                                                    <  2:2518195/149‑1 (MQ=255)

                                                                             gTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCg                                                                   >  1:1344073/1‑149 (MQ=255)

                                                                             gTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCg                                                                   >  1:3090674/1‑149 (MQ=255)

                                                                                  gTTCATCGACTTTATCCTGACAATCGACATCGCTGCGATAAGCCGCCAGTCCCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACAAGATCGCGTTAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGc                                                              <  1:2128619/149‑1 (MQ=255)

                                                                                    tCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCTCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCAc                                                            <  2:2415837/149‑1 (MQ=255)

                                                                                       tCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTg                                                         >  1:3278825/1‑149 (MQ=255)

                                                                                       tCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTg                                                         >  2:2987404/1‑149 (MQ=255)

                                                                                        cGACTTTATCCTGACAATCGACATAGCTGCTATAATCCGCCAGTACCTGGTAGTGATCGCCGATGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGa                                                        <  1:406486/149‑1 (MQ=255)

                                                                                        cGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGa                                                        <  1:406692/149‑1 (MQ=255)

                                                                                          aCTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATg                                                      >  2:1478166/1‑149 (MQ=255)

                                                                                          aCTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATg                                                      >  2:3522953/1‑149 (MQ=255)

                                                                                            tttATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCa                                                    <  2:493647/149‑1 (MQ=255)

                                                                                                tCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGctc                                                <  2:3627204/149‑1 (MQ=255)

                                                                                                 ccTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGctcc                                               <  2:1702370/149‑1 (MQ=255)

                                                                                                     aCAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCAt                                           <  2:3071457/149‑1 (MQ=255)

                                                                                                      cAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATc                                          <  2:2310286/149‑1 (MQ=255)

                                                                                                       aaTCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCt                                         <  2:2586339/149‑1 (MQ=255)

                                                                                                          cGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTtc                                      <  2:3659394/149‑1 (MQ=255)

                                                                                                          cGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTtc                                      <  2:946860/149‑1 (MQ=255)

                                                                                                           gACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTtct                                     >  2:735971/1‑149 (MQ=255)

                                                                                                               tAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCgta                                 <  2:4037238/149‑1 (MQ=255)

                                                                                                                   tGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTAt                             >  2:2701536/1‑149 (MQ=255)

                                                                                                                      gATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCa                          <  1:3767370/149‑1 (MQ=255)

                                                                                                                      gATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCa                          >  1:1039111/1‑149 (MQ=255)

                                                                                                                      gATAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCa                          <  2:2507036/149‑1 (MQ=255)

                                                                                                                       aTAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCAc                         >  1:2715913/1‑149 (MQ=255)

                                                                                                                        tAATCCGCCAGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTAtt                            <  1:450358/145‑1 (MQ=255)

                                                                                                                                cAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTgg                >  2:2660838/1‑148 (MQ=255)

                                                                                                                                cAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACAAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGt                <  1:1489214/149‑1 (MQ=255)

                                                                                                                                 aGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTa               >  1:4040110/1‑149 (MQ=255)

                                                                                                                                 aGTACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTa               >  1:997550/1‑149 (MQ=255)

                                                                                                                                   tACCTGGTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGc             >  2:3251937/1‑149 (MQ=255)

                                                                                                                                      cTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCt          <  2:667297/149‑1 (MQ=255)

                                                                                                                                      cTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCt          <  2:3826099/149‑1 (MQ=255)

                                                                                                                                        ggTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTga        <  1:3433065/149‑1 (MQ=255)

                                                                                                                                        ggTAGTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTga        <  2:3865756/149‑1 (MQ=255)

                                                                                                                                         gTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTgac       >  2:3408008/1‑149 (MQ=255)

                                                                                                                                         gTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTgac       <  1:2095356/149‑1 (MQ=255)

                                                                                                                                           aGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTgacga     <  1:3743382/149‑1 (MQ=255)

                                                                                                                                           aGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTgacga     <  1:3743407/149‑1 (MQ=255)

                                                                                                                                            gTGATAGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTgacgac    >  1:2795541/1‑149 (MQ=255)

ATAGTACGGTCAGAAGAGAAGTAGCCCATATTGGCAATGTTCAGCATCGCTTTTGCGGTCCACTCTTCCTGAAGCTCGTAGAGTTCATCGACTTTATCCTGACAATCGACATAGCTGCGATAATCCGCCAGTACCTGGTAGTGATCGCCGAAGTTGATCAGCGAATCAACCAGATCGCGATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGC  >  CP009273/3557543‑3557833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`