Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,561,574 C→A 100% W372L (TGG→TTG)  glgC ← glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,561,5740CA99.6% 765.4 / ‑4.4 232W372L (TGG→TTG) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (117/114);  total (117/115)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

TTTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTAC  >  CP009273/3561428‑3561718
                                                                                                                                                  |                                                                                                                                                
tttCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACTATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAAt                                                                                                                                                >  1:1230241/1‑149 (MQ=255)
tttCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAAt                                                                                                                                                >  1:598194/1‑149 (MQ=255)
  tCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATAc                                                                                                                                              <  1:166976/149‑1 (MQ=255)
   cgcgcgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACt                                                                                                                                             <  2:1507347/149‑1 (MQ=255)
      gcgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTcc                                                                                                                                          <  2:506514/149‑1 (MQ=255)
        gTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCgg                                                                                                                                        >  1:1032162/1‑149 (MQ=255)
        gTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCgg                                                                                                                                        >  1:3475174/1‑149 (MQ=255)
        gTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCgg                                                                                                                                        >  1:1324627/1‑149 (MQ=255)
         ttACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGt                                                                                                                                       <  2:1246325/149‑1 (MQ=255)
          tACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTa                                                                                                                                      <  2:2864421/149‑1 (MQ=255)
            cccgCACGTAGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAAGAACACAAGCACGATCGCTGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAAc                                                                                                                                    <  1:822435/146‑1 (MQ=255)
               gCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAAt                                                                                                                                 <  1:3807168/149‑1 (MQ=255)
                 aCGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAc                                                                                                                               <  2:326858/149‑1 (MQ=255)
                   gATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcgg                                                                                                                             >  2:4025140/1‑149 (MQ=255)
                    aTGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcggc                                                                                                                            >  1:1264320/1‑149 (MQ=255)
                     tGCCTTCTTCTGAACGATAGAAACTACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATANCACAAGCGCGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcggcg                                                                                                                           >  1:2086422/1‑149 (MQ=255)
                     tGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcggcg                                                                                                                           >  1:2697164/1‑149 (MQ=255)
                        cttcttCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGaa                                                                                                                        >  2:2460173/1‑149 (MQ=255)
                         ttcttcTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAAt                                                                                                                       <  2:1887155/149‑1 (MQ=255)
                           cttctGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCa                                                                                                                     >  2:2633750/1‑149 (MQ=255)
                            ttctGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAAGAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCaa                                                                                                                    <  2:3559844/149‑1 (MQ=255)
                                gAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGtt                                                                                                                <  1:98307/149‑1 (MQ=255)
                                gAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGtt                                                                                                                >  1:2827517/1‑149 (MQ=255)
                                gAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGtt                                                                                                                <  1:521525/149‑1 (MQ=255)
                                  actaTACAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGc                                                                                                              <  1:1394732/146‑1 (MQ=255)
                                  aCGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGc                                                                                                              >  2:607190/1‑149 (MQ=255)
                                   cGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCa                                                                                                             <  1:2248150/149‑1 (MQ=255)
                                     aTAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAga                                                                                                           >  1:519850/1‑149 (MQ=255)
                                      tAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaa                                                                                                          >  1:3814377/1‑149 (MQ=255)
                                       aGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaat                                                                                                         <  1:3691797/149‑1 (MQ=255)
                                       aGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaat                                                                                                         <  1:1644950/149‑1 (MQ=255)
                                       aGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaat                                                                                                         <  1:3217365/149‑1 (MQ=255)
                                           acgacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatgaat                                                                                                     >  1:2961195/1‑149 (MQ=255)
                                            cgacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAAtt                                                                                                    <  2:2114889/149‑1 (MQ=255)
                                              acgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCa                                                                                                  <  1:671761/149‑1 (MQ=255)
                                              acgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCa                                                                                                  <  1:3523718/149‑1 (MQ=255)
                                              acgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCa                                                                                                  >  2:2302903/1‑149 (MQ=255)
                                                 tGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcgc                                                                                               >  2:2669044/1‑149 (MQ=255)
                                                   cATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGa                                                                                             >  2:1876753/1‑149 (MQ=255)
                                                       ttCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcgc                                                                                         >  2:1360839/1‑149 (MQ=255)
                                                       gtCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcgc                                                                                         <  2:2163941/148‑1 (MQ=255)
                                                         ccTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcgcgc                                                                                       >  2:914425/1‑149 (MQ=255)
                                                         ccTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcgcgc                                                                                       >  1:338008/1‑149 (MQ=255)
                                                            ctGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCgag                                                                                    >  1:110236/1‑149 (MQ=255)
                                                             tGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCgaga                                                                                   >  1:1880349/1‑149 (MQ=255)
                                                              gCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGCGGACGCAGCGGCGCAGACGGCAAGAGCGACCTACCAATACTTCCGGTTACAATACGGCGGAATCAATGTTTCAGAATGAATTCACGCGAACGCGCGagaa                                                                                  <  2:3048741/149‑1 (MQ=255)
                                                                 ttttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacag                                                                               >  1:1048573/1‑149 (MQ=255)
                                                                 ttttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacag                                                                               >  1:1962952/1‑149 (MQ=255)
                                                                  tttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacaga                                                                              <  2:1032162/149‑1 (MQ=255)
                                                                   ttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacagaa                                                                             <  2:1436754/149‑1 (MQ=255)
                                                                      aCCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACgg                                                                          <  2:3285763/149‑1 (MQ=255)
                                                                        cAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGAc                                                                        >  1:1845478/1‑149 (MQ=255)
                                                                        cAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGAc                                                                        >  1:2650010/1‑149 (MQ=255)
                                                                         aaTCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACt                                                                       >  2:75504/1‑149 (MQ=255)
                                                                          aTCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTg                                                                      >  2:3997306/1‑149 (MQ=255)
                                                                          aTCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTg                                                                      >  2:3878725/1‑149 (MQ=255)
                                                                            cACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGca                                                                    >  1:1348092/1‑149 (MQ=255)
                                                                               cATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcacca                                                                 >  1:2347222/1‑149 (MQ=255)
                                                                                  gCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcaccacca                                                              >  2:3199356/1‑149 (MQ=255)
                                                                                        cGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACCGCGCGAGAACAGAACGGACTGCACCACCACCGacc                                                        >  2:2589555/1‑147 (MQ=255)
                                                                                         ggAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACCGCGCGAGAACAGAACGGACTGCACCACCACCGAAcc                                                       >  1:1137846/1‑149 (MQ=255)
                                                                                         ggAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAcc                                                       >  1:3983551/1‑149 (MQ=255)
                                                                                          gAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCg                                                      >  2:793124/1‑149 (MQ=255)
                                                                                           aaTAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCgg                                                     >  1:2368203/1‑149 (MQ=255)
                                                                                            aTAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGga                                                    <  2:3583826/149‑1 (MQ=255)
                                                                                              aaCACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGgaga                                                  >  1:461283/1‑149 (MQ=255)
                                                                                                  caAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcac                                              >  2:1262132/1‑149 (MQ=255)
                                                                                                    aGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacac                                            >  2:4080734/1‑149 (MQ=255)
                                                                                                    aGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacac                                            <  1:2630509/149‑1 (MQ=255)
                                                                                                      cACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACaa                                          >  1:3745337/1‑149 (MQ=255)
                                                                                                       aCGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAc                                         <  2:1827568/149‑1 (MQ=255)
                                                                                                       aCGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAc                                         <  2:3865343/149‑1 (MQ=255)
                                                                                                       aCGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAc                                         <  1:3103819/149‑1 (MQ=255)
                                                                                                         gatcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccg                                       <  1:1050700/149‑1 (MQ=255)
                                                                                                          atcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGTATGAATTCACGCGACCGCGCGAGAACAGAACGGACTGCACCACCACCGACCCGGAGATCACGCAAccgc                                      >  2:3377421/1‑149 (MQ=255)
                                                                                                                  cGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAAcc                              >  1:275578/1‑149 (MQ=255)
                                                                                                                   gCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCa                             <  2:3814377/149‑1 (MQ=255)
                                                                                                                   gCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCa                             >  1:911370/1‑149 (MQ=255)
                                                                                                                     aGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGt                           <  1:3638217/149‑1 (MQ=255)
                                                                                                                     aGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGt                           >  1:1785562/1‑149 (MQ=255)
                                                                                                                     aGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGt                           <  1:3353737/149‑1 (MQ=255)
                                                                                                                       cggcgCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGa                         <  2:3138945/149‑1 (MQ=255)
                                                                                                                       cggcgCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGa                         <  2:704577/149‑1 (MQ=255)
                                                                                                                        ggcgCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAg                        >  2:3322177/1‑149 (MQ=255)
                                                                                                                        ggcgCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAg                        <  1:3334161/149‑1 (MQ=255)
                                                                                                                            cAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACTACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTaa                    <  2:1223602/149‑1 (MQ=255)
                                                                                                                              gACTGCACGAGCGACCTACAAATACTTCCGGTAACAAAACGGCGGACTCAAAGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCAACGAAACGGAGATCACACAACCGCCCGAAACCAGTCAGATAAgg                  >  2:1688397/1‑149 (MQ=255)
                                                                                                                              gACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAgg                  >  2:1856190/1‑149 (MQ=255)
                                                                                                                               aCGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAggg                 <  1:3560788/149‑1 (MQ=255)
                                                                                                                                  gCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCa              >  1:2145427/1‑149 (MQ=255)
                                                                                                                                  gCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCa              >  1:341369/1‑149 (MQ=255)
                                                                                                                                  gCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCa              <  1:1661726/149‑1 (MQ=255)
                                                                                                                                       aGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCg         <  1:597063/149‑1 (MQ=255)
                                                                                                                                        gCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGt        <  1:793062/149‑1 (MQ=255)
                                                                                                                                        gCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGt        >  2:1635801/1‑149 (MQ=255)
                                                                                                                                          gACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACACCCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTgg      <  2:1417059/149‑1 (MQ=255)
                                                                                                                                          gACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTgg      <  1:2743565/149‑1 (MQ=255)
                                                                                                                                          gACCTACAAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTgg      <  2:2398766/149‑1 (MQ=255)
                                                                                                                                           aCCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGc     <  1:3301021/149‑1 (MQ=255)
                                                                                                                                           aCCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGc     <  1:3997306/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTAC  >  CP009273/3561428‑3561718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑