Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,562,778 A→T 7.4% A634A (GCT→GCA)  glgX ← glycogen debranching enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,562,7780AT 7.4% 776.0 / 40.0 258A634A (GCT→GCA) glgXglycogen debranching enzyme
Reads supporting (aligned to +/- strand):  ref base A (130/108);  new base T (11/8);  total (142/116)
Fisher's exact test for biased strand distribution p-value = 8.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 259 total aligned reads displayed.

ATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTGCCACTCATCCGTGCTTAAAGGTT  >  CP009273/3562631‑3562926
                                                                                                                                                   |                                                                                                                                                    
aTTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAg                                                                                                                                                     <  2:1234030/149‑1 (MQ=255)
aTTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAg                                                                                                                                                     >  1:1423905/1‑149 (MQ=255)
 tttCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCCGc                                                                                                                                                    >  2:3558441/1‑149 (MQ=255)
 tttCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGc                                                                                                                                                    <  1:1171399/149‑1 (MQ=255)
   tCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGa                                                                                                                                                  >  1:255508/1‑149 (MQ=255)
     aaTGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAAt                                                                                                                                                <  1:142524/149‑1 (MQ=255)
     aaTGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAAt                                                                                                                                                <  2:2106685/149‑1 (MQ=255)
     aaTGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAAt                                                                                                                                                <  2:2106606/149‑1 (MQ=255)
       tGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATgg                                                                                                                                              <  1:2207982/149‑1 (MQ=255)
        ggCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCTGCGAATggg                                                                                                                                             <  1:2608020/149‑1 (MQ=255)
        ggCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATggg                                                                                                                                             <  1:443488/149‑1 (MQ=255)
         gCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATgggg                                                                                                                                            >  2:2652171/1‑149 (MQ=255)
          cAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATggggg                                                                                                                                           <  2:1930759/149‑1 (MQ=255)
           aGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGa                                                                                                                                          <  1:3347924/149‑1 (MQ=255)
            gCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGaa                                                                                                                                         <  1:1351333/149‑1 (MQ=255)
            gCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGaa                                                                                                                                         <  1:273351/149‑1 (MQ=255)
               ggCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATgg                                                                                                                                      >  2:2623494/1‑149 (MQ=255)
                gcgcgcCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGc                                                                                                                                     >  1:311965/1‑149 (MQ=255)
                  gcgcCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGt                                                                                                                                   >  2:3772470/1‑149 (MQ=255)
                     ccAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGcc                                                                                                                                >  1:1144758/1‑149 (MQ=255)
                     ccAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGcc                                                                                                                                <  1:2017617/149‑1 (MQ=255)
                     ccAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGcc                                                                                                                                <  2:657233/149‑1 (MQ=255)
                           ttAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTccc                                                                                                                          <  2:1688826/149‑1 (MQ=255)
                            tAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTcccc                                                                                                                         <  2:1134001/149‑1 (MQ=255)
                            tAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTcccc                                                                                                                         <  1:3532486/149‑1 (MQ=255)
                            tAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTcccc                                                                                                                         >  2:3173605/1‑149 (MQ=255)
                               gTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCcagc                                                                                                                      <  1:521812/149‑1 (MQ=255)
                                tGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCcagca                                                                                                                     >  2:1494866/1‑149 (MQ=255)
                                   tCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGt                                                                                                                  >  1:1946624/1‑149 (MQ=255)
                                    cGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACGCAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTa                                                                                                                 >  1:826073/1‑149 (MQ=255)
                                     gTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTaa                                                                                                                >  1:2840090/1‑149 (MQ=255)
                                      ttcttcTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTaaa                                                                                                               >  1:2345658/1‑149 (MQ=255)
                                      ttcttcTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCAAGCAGGTaaa                                                                                                               >  2:3802145/1‑149 (MQ=255)
                                         ttctCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACa                                                                                                            >  2:3110397/1‑149 (MQ=255)
                                           ctctAGACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAAt                                                                                                          <  1:2925712/149‑1 (MQ=255)
                                             ctAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAAtct                                                                                                        <  1:2321009/149‑1 (MQ=255)
                                               aaccTAACCATGACTCCCTCCTATTTTATCATCTCTGGAACACACACAATCCGTGTGCCGGTCCCTGCCAGACAGCCGTAATCACTGGGTGATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATctct                                                                                                      <  2:1512248/146‑1 (MQ=255)
                                                aaCTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTg                                                                                                     <  1:33622/149‑1 (MQ=255)
                                                aaCTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTg                                                                                                     >  1:2692428/1‑149 (MQ=255)
                                                aaCTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTg                                                                                                     <  1:2110358/149‑1 (MQ=255)
                                                 aCTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCTGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGt                                                                                                    >  1:1758654/1‑149 (MQ=255)
                                                    aaCCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGACTGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTAc                                                                                                 >  2:1219740/1‑149 (MQ=255)
                                                    aaCCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTAc                                                                                                 >  2:3530403/1‑149 (MQ=255)
                                                        aTGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCa                                                                                             >  1:3705616/1‑149 (MQ=255)
                                                        aTGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCa                                                                                             <  2:1607250/149‑1 (MQ=255)
                                                        aTGACTAACTCCTGTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCa                                                                                             <  2:2671017/149‑1 (MQ=255)
                                                         tGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCTGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCaa                                                                                            >  2:3917346/1‑149 (MQ=255)
                                                           aCTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCTGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGc                                                                                          >  1:3081086/1‑149 (MQ=255)
                                                            cTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCg                                                                                         >  1:3621781/1‑149 (MQ=255)
                                                             tAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGt                                                                                        <  2:3432198/149‑1 (MQ=255)
                                                              aaCTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTg                                                                                       >  2:3146447/1‑149 (MQ=255)
                                                              aaCTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTg                                                                                       >  1:2715944/1‑149 (MQ=255)
                                                               aCTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTgg                                                                                      >  2:405712/1‑149 (MQ=255)
                                                               aCTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTgg                                                                                      <  2:133528/149‑1 (MQ=255)
                                                                cTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGc                                                                                     >  1:1777298/1‑149 (MQ=255)
                                                                      ttttATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAAt                                                                               <  1:149170/149‑1 (MQ=255)
                                                                       tttATCATCTCTGGAACACACACACTCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAAtt                                                                              <  1:3587257/149‑1 (MQ=255)
                                                                         tATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGc                                                                            >  2:2337430/1‑149 (MQ=255)
                                                                            catcTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGAt                                                                         <  1:3963444/149‑1 (MQ=255)
                                                                             atcTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATc                                                                        <  1:903745/149‑1 (MQ=255)
                                                                             atcTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATc                                                                        <  1:1505550/149‑1 (MQ=255)
                                                                              tctcTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCa                                                                       >  1:3001636/1‑149 (MQ=255)
                                                                              tctcTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCa                                                                       >  1:451809/1‑149 (MQ=255)
                                                                                  tGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCaaaaa                                                                   >  2:2875914/1‑149 (MQ=255)
                                                                                   ggAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAg                                                                  <  2:4076706/149‑1 (MQ=255)
                                                                                       cacacacaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGAt                                                              <  1:2624319/149‑1 (MQ=255)
                                                                                           cacaATCCGTGTGCAGGTCCCTGCCATACAGCCGTAATCACAGGGTTATCCTCTCCTGCGAAAGGGGCAAAGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGAGCCGTTAATTGCTATCAAAAAGCGATCCga                                                          >  2:3745137/1‑149 (MQ=255)
                                                                                           cacaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCga                                                          <  1:3055867/149‑1 (MQ=255)
                                                                                           cacaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCga                                                          <  2:1693233/149‑1 (MQ=255)
                                                                                           cacaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCga                                                          >  1:2376444/1‑149 (MQ=255)
                                                                                           cacaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCga                                                          >  2:2740274/1‑149 (MQ=255)
                                                                                            acaATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCgag                                                         >  1:750122/1‑149 (MQ=255)
                                                                                             caATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCTGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCgaga                                                        <  2:2976512/149‑1 (MQ=255)
                                                                                               aTCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGc                                                      >  1:779033/1‑149 (MQ=255)
                                                                                                tCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCa                                                     <  1:3213209/149‑1 (MQ=255)
                                                                                                         aGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCa                                            >  2:2510321/1‑149 (MQ=255)
                                                                                                         aGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCa                                            >  1:282292/1‑149 (MQ=255)
                                                                                                          ggTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAg                                           <  2:109194/149‑1 (MQ=255)
                                                                                                            tCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCt                                         >  2:833711/1‑149 (MQ=255)
                                                                                                             cccTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTg                                        <  1:1601709/149‑1 (MQ=255)
                                                                                                              ccTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGt                                       <  1:592953/149‑1 (MQ=255)
                                                                                                               cTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGtt                                      <  2:2038137/149‑1 (MQ=255)
                                                                                                                   cAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCgg                                  >  1:4045290/1‑149 (MQ=255)
                                                                                                                     gACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGcc                                >  1:2305771/1‑149 (MQ=255)
                                                                                                                     gACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCAGAGAGCAGAATTTGCAGCTGTTTCGGcc                                >  1:3936618/1‑149 (MQ=255)
                                                                                                                      aCAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGccc                               >  1:677431/1‑149 (MQ=255)
                                                                                                                      aCAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGccc                               <  1:2281380/149‑1 (MQ=255)
                                                                                                                        aGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGt                             >  2:1979030/1‑149 (MQ=255)
                                                                                                                         gCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGtt                            <  2:451809/149‑1 (MQ=255)
                                                                                                                          ccGTAATCACTGGGTTATCCTCTCCTGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGttt                           <  2:1758654/149‑1 (MQ=255)
                                                                                                                          ccGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGttt                           <  1:1852716/149‑1 (MQ=255)
                                                                                                                             tAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTGc                        >  2:3360076/1‑149 (MQ=255)
                                                                                                                                          aTCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTGCCACTCATCCGTGc           <  2:255508/149‑1 (MQ=255)
                                                                                                                                          aTCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTGCCACTCATCCGTGc           <  2:1423905/149‑1 (MQ=255)
                                                                                                                                           tCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTGCCACTCATCCGTGCt          >  2:535290/1‑149 (MQ=255)
                                                                                                                                               ctcCTGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTGCCACTCATCCGTGCTTaaa      >  1:848730/1‑149 (MQ=255)
                                                                                                                                               ctcCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTGCCACTCATCCGTGCTTaaa      >  1:3685072/1‑149 (MQ=255)
                                                                                                                                                tcCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTGCCACTCATCCGTGCTTAAAg     >  1:2155021/1‑149 (MQ=255)
                                                                                                                                                tcCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTGCCACTCATCCGTGCTTAAAg     >  1:1338908/1‑149 (MQ=255)
                                                                                                                                                  cAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTGCCACTCATCCGTGCTTAAAGGt   >  2:94607/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTTTTATCATCTCTGGAACACACACAATCCGTGTGCAGGTCCCTGCCAGACAGCCGTAATCACTGGGTTATCCTCTCCAGCGAATGGGGGAATGGCGTGCCACTCCCCAGCAGGTAAAACAATCTCTGTTACCTCAAGCGTGGCGTTAATTGCGATCAAAAAGCGATCCGAGAGCAGAATTTGCAGCTGTTTCGGCCCGTTTTGCCACTCATCCGTGCTTAAAGGTT  >  CP009273/3562631‑3562926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑