Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,590,636 G→T 100% Y95* (TAC→TAA)  livK ← leucine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,590,6360GT97.1% 900.8 / 13.5 293Y95* (TAC→TAA) livKleucine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (4/5);  new base T (151/133);  total (155/138)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 293 total aligned reads displayed.

TCCAGCCCGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACGCGCGCCG  >  CP009273/3590490‑3590780
                                                                                                                                                  |                                                                                                                                                
tCCAGCCCGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTa                                                                                                                                                <  1:1797719/149‑1 (MQ=255)
  cAGCCCGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTAtt                                                                                                                                              <  1:3700589/149‑1 (MQ=255)
  cAGCCCGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTAtt                                                                                                                                              >  1:2372118/1‑149 (MQ=255)
      ccGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAAt                                                                                                                                          >  1:3160174/1‑149 (MQ=255)
        ggCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGc                                                                                                                                        <  2:2599700/149‑1 (MQ=255)
             tACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCa                                                                                                                                   >  1:1247289/1‑149 (MQ=255)
             tACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCa                                                                                                                                   >  1:3124782/1‑149 (MQ=255)
              aCGCATATTGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCAt                                                                                                                                  <  2:3105638/149‑1 (MQ=255)
                 cATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTaa                                                                                                                               <  1:936081/149‑1 (MQ=255)
                 cATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTaa                                                                                                                               <  1:3310921/149‑1 (MQ=255)
                   tAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACg                                                                                                                             >  1:2244874/1‑149 (MQ=255)
                     aTGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGAt                                                                                                                           <  1:3257517/149‑1 (MQ=255)
                      tgtgTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGagt                                                                                                                          >  2:2212230/1‑147 (MQ=255)
                       gtgtTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTACTGCCGTCATTAACGAttt                                                                                                                         <  2:2357412/149‑1 (MQ=255)
                       gtgtTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGAttt                                                                                                                         >  2:539750/1‑149 (MQ=255)
                          ttGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTttgt                                                                                                                      <  2:3319814/149‑1 (MQ=255)
                            gATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTttgttg                                                                                                                    >  1:2154502/1‑149 (MQ=255)
                                aCCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGa                                                                                                                <  1:427748/149‑1 (MQ=255)
                                  cgcgTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGAcc                                                                                                              <  1:629619/149‑1 (MQ=255)
                                        gggTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACg                                                                                                        >  1:1955391/1‑149 (MQ=255)
                                           tCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCt                                                                                                     >  2:2748781/1‑149 (MQ=255)
                                           tCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCt                                                                                                     >  2:502518/1‑149 (MQ=255)
                                            cAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCtt                                                                                                    >  2:2064859/1‑149 (MQ=255)
                                             aGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTg                                                                                                   >  1:373172/1‑149 (MQ=255)
                                                    ggTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCggg                                                                                            <  1:852465/149‑1 (MQ=255)
                                                    ggTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCggg                                                                                            <  1:433125/149‑1 (MQ=255)
                                                         gTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCa                                                                                       <  1:2107642/149‑1 (MQ=255)
                                                         gTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCa                                                                                       <  2:1594290/149‑1 (MQ=255)
                                                          tCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCAt                                                                                      >  2:2919918/1‑149 (MQ=255)
                                                             cTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGcg                                                                                   >  1:3614329/1‑149 (MQ=255)
                                                              tCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGcgt                                                                                  <  1:24595/149‑1 (MQ=255)
                                                                ccGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGcgtcg                                                                                >  1:897269/1‑149 (MQ=255)
                                                                  ggCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCgtcgtc                                                                              <  1:2047816/149‑1 (MQ=255)
                                                                     gagaTCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCtta                                                                           <  2:103076/149‑1 (MQ=255)
                                                                     gagaTCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCata                                                                           >  1:1296748/1‑149 (MQ=255)
                                                                     gagaTCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCata                                                                           <  2:2252907/149‑1 (MQ=255)
                                                                      agaTCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCatat                                                                          >  1:2616233/1‑149 (MQ=255)
                                                                        atcatcAGAATACCTTCGTCTACATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATTACCAATAACTTATTTAATGACGTCATTAACTATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGAGTCGTCATAtta                                                                        >  1:2909051/1‑148 (MQ=255)
                                                                          catcaGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCa                                                                      >  2:3166379/1‑149 (MQ=255)
                                                                           atcaGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCAAc                                                                     >  2:126255/1‑149 (MQ=255)
                                                                            tcaGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACg                                                                    <  1:3638287/149‑1 (MQ=255)
                                                                             caGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGc                                                                   <  1:673002/149‑1 (MQ=255)
                                                                              aGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGcc                                                                  >  2:3675449/1‑149 (MQ=255)
                                                                                aaTACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCaa                                                                <  1:3028407/149‑1 (MQ=255)
                                                                                  tACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAAcc                                                              <  1:3196093/149‑1 (MQ=255)
                                                                                  tACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAAcc                                                              >  2:4056621/1‑149 (MQ=255)
                                                                                    ccTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAg                                                            <  2:3160174/149‑1 (MQ=255)
                                                                                    ccTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCAGGTCGCATGCGTCGTCATATTCCACGCCAACCAg                                                            >  1:527138/1‑149 (MQ=255)
                                                                                     cTTCGTCTTAATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGt                                                           >  2:381685/1‑149 (MQ=255)
                                                                                         gTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTAt                                                       >  1:562936/1‑149 (MQ=255)
                                                                                         gTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTAt                                                       >  1:3165203/1‑149 (MQ=255)
                                                                                         gTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTAt                                                       >  2:1274547/1‑149 (MQ=255)
                                                                                          tCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATc                                                      <  1:2764417/149‑1 (MQ=255)
                                                                                          tCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATc                                                      <  2:198829/149‑1 (MQ=255)
                                                                                          tCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATc                                                      <  1:1034379/149‑1 (MQ=255)
                                                                                              cATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGccc                                                  >  1:759884/1‑149 (MQ=255)
                                                                                               aTAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCt                                                 <  2:748069/149‑1 (MQ=255)
                                                                                                 aGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTa                                               >  2:728906/1‑149 (MQ=255)
                                                                                                   atatCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAAt                                             <  1:2736932/149‑1 (MQ=255)
                                                                                                    tatCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAAtt                                            >  1:1068760/1‑149 (MQ=255)
                                                                                                     atCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTc                                           >  2:2576984/1‑149 (MQ=255)
                                                                                                      tCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTcc                                          >  2:1343412/1‑149 (MQ=255)
                                                                                                          aCGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCt                                      <  2:3596773/149‑1 (MQ=255)
                                                                                                           cGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCtt                                     <  2:967083/149‑1 (MQ=255)
                                                                                                           cGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCtt                                     <  1:2785291/149‑1 (MQ=255)
                                                                                                            gCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCttt                                    <  1:1692351/149‑1 (MQ=255)
                                                                                                            gCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCttt                                    >  2:44515/1‑149 (MQ=255)
                                                                                                            gCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCttt                                    >  1:4050523/1‑149 (MQ=255)
                                                                                                               ggCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGc                                 >  2:368643/1‑149 (MQ=255)
                                                                                                                 cTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGACGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCAt                               <  2:3064845/149‑1 (MQ=255)
                                                                                                                   gggTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTa                             >  1:2275112/1‑149 (MQ=255)
                                                                                                                      tAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATg                          <  1:2150398/149‑1 (MQ=255)
                                                                                                                         aagaagacCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCt                       <  2:3036240/149‑1 (MQ=255)
                                                                                                                         aagaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCt                       <  2:1138070/149‑1 (MQ=255)
                                                                                                                         aagaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCt                       <  2:2909051/149‑1 (MQ=255)
                                                                                                                         aagaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCt                       >  2:14180/1‑149 (MQ=255)
                                                                                                                         aagaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCt                       >  2:1751100/1‑149 (MQ=255)
                                                                                                                         aagaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCt                       <  2:3189647/149‑1 (MQ=255)
                                                                                                                          agaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCtt                      <  2:2477843/149‑1 (MQ=255)
                                                                                                                          agaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCAAGCCAACCAGTTTATCGCCCTTAATTCACCCTTTGGCATTAAAGTCtt                      >  1:584532/1‑149 (MQ=255)
                                                                                                                           gaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCttt                     >  2:2548207/1‑149 (MQ=255)
                                                                                                                            aagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTa                    >  2:1485127/1‑149 (MQ=255)
                                                                                                                              gaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAAt                  <  2:1845809/149‑1 (MQ=255)
                                                                                                                              gaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTAAAt                  >  1:1641905/1‑149 (MQ=255)
                                                                                                                               aaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAAtt                 <  1:1413538/149‑1 (MQ=255)
                                                                                                                                    aGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTg            >  1:2229023/1‑149 (MQ=255)
                                                                                                                                     gATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCTGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGACTGa           >  2:3523086/1‑149 (MQ=255)
                                                                                                                                     gATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGa           <  2:2154114/149‑1 (MQ=255)
                                                                                                                                       tGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGAcg         <  1:2405750/149‑1 (MQ=255)
                                                                                                                                        gACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGAcgc        <  2:1257522/149‑1 (MQ=255)
                                                                                                                                         cccAGTAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGAcgcg       >  1:2163253/2‑149 (MQ=255)
                                                                                                                                         aCCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGAcgcg       >  2:3397911/1‑149 (MQ=255)
                                                                                                                                         aCCAATAACGTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGAcgcg       >  2:628775/1‑149 (MQ=255)
                                                                                                                                          ccAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCACCCTTTGGCATTAATGTCTTTAATTGCCTGAcgcgc      >  1:3779904/1‑149 (MQ=255)
                                                                                                                                           cAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGAcgcgcg     >  1:4004008/1‑149 (MQ=255)
                                                                                                                                            aaTAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACgcgcgc    >  2:3000369/1‑149 (MQ=255)
                                                                                                                                             aTAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACGCGCGcc   >  1:3107388/1‑149 (MQ=255)
                                                                                                                                             aTAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACGCGCGcc   >  1:3301417/1‑149 (MQ=255)
                                                                                                                                              tAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACGCGCGCCg  >  1:3482438/1‑149 (MQ=255)
                                                                                                                                              tAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACGCGCGCCg  >  2:154994/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCCAGCCCGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACGCGCGCCG  >  CP009273/3590490‑3590780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑