Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,590,906 C→A 100% A5A (GCG→GCT)  livK ← leucine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,590,9060CA98.6% 956.3 / 2.5 294A5A (GCG→GCT) livKleucine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base A (118/171);  total (119/175)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 294 total aligned reads displayed.

TTAATTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTATTTTGTGATAATA  >  CP009273/3590760‑3591051
                                                                                                                                                  |                                                                                                                                                 
   aTTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCAtt                                                                                                                                              >  2:3571482/1‑149 (MQ=255)
   aTTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCAtt                                                                                                                                              >  1:1126685/1‑149 (MQ=255)
   aTTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCAtt                                                                                                                                              >  2:198769/1‑149 (MQ=255)
    ttGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTc                                                                                                                                             <  1:368579/149‑1 (MQ=255)
      gCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCg                                                                                                                                           >  1:855672/1‑149 (MQ=255)
       ccTGACGCGCGCCGTTATATTCCATATCGCCCCTCTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGt                                                                                                                                          <  1:194639/149‑1 (MQ=255)
        cTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGtt                                                                                                                                         >  2:3938983/1‑149 (MQ=255)
        cTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGtt                                                                                                                                         >  1:401911/1‑149 (MQ=255)
         tGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGttt                                                                                                                                        >  1:2939806/1‑149 (MQ=255)
         tGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGttt                                                                                                                                        <  1:2576022/149‑1 (MQ=255)
          gACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTc                                                                                                                                       >  2:1717108/1‑149 (MQ=255)
          gACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTc                                                                                                                                       >  1:1891800/1‑149 (MQ=255)
             gcgcgcCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATa                                                                                                                                    <  1:1332723/149‑1 (MQ=255)
             gcgcgcCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATa                                                                                                                                    >  1:1811691/1‑149 (MQ=255)
             gcgcgcCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATa                                                                                                                                    <  1:3379495/149‑1 (MQ=255)
             gcgcgcCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATa                                                                                                                                    <  2:3317787/149‑1 (MQ=255)
             gcgcgcCGTTAAATTCCATATAGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATa                                                                                                                                    >  1:4052546/1‑149 (MQ=255)
               gcgcCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGt                                                                                                                                  <  2:1794312/149‑1 (MQ=255)
               gcgcCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGt                                                                                                                                  <  1:1263308/149‑1 (MQ=255)
                  ccGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCaa                                                                                                                               >  2:1760727/1‑149 (MQ=255)
                     ttAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCaaaaa                                                                                                                            >  2:3376075/1‑149 (MQ=255)
                     ttAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCaaaaa                                                                                                                            >  1:3596453/1‑149 (MQ=255)
                     ttAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCaaaaa                                                                                                                            <  1:1560237/149‑1 (MQ=255)
                     ttAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCaaaaa                                                                                                                            >  2:486298/1‑149 (MQ=255)
                     ttAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGAGAGTTTTAGCATTCCGTGTCATAGTCaaaaa                                                                                                                            >  2:2297197/1‑149 (MQ=255)
                          ttCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATcccc                                                                                                                       >  2:904821/1‑149 (MQ=255)
                          ttCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATcccc                                                                                                                       <  2:2163253/149‑1 (MQ=255)
                           tCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCa                                                                                                                      <  2:1573307/149‑1 (MQ=255)
                            ccATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCAt                                                                                                                     <  1:1222197/149‑1 (MQ=255)
                                atCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGt                                                                                                                 >  1:1986353/1‑149 (MQ=255)
                                atCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGt                                                                                                                 >  1:3433786/1‑149 (MQ=255)
                                   gCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGAt                                                                                                              <  1:3000369/149‑1 (MQ=255)
                                   gCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGAt                                                                                                              <  1:3182189/149‑1 (MQ=255)
                                      ccACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGAtgtt                                                                                                           <  2:1004179/149‑1 (MQ=255)
                                      ccACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGAtgtt                                                                                                           <  1:2919771/149‑1 (MQ=255)
                                        aCTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGAtgttgt                                                                                                         <  2:2893111/149‑1 (MQ=255)
                                          tGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGAtgttgtgt                                                                                                       <  2:132937/149‑1 (MQ=255)
                                             gCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGc                                                                                                    <  2:1299658/149‑1 (MQ=255)
                                             gCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGc                                                                                                    <  1:1407972/149‑1 (MQ=255)
                                              cAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCt                                                                                                   <  2:3847830/149‑1 (MQ=255)
                                              cAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCt                                                                                                   <  2:3820745/149‑1 (MQ=255)
                                               aaTCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCtt                                                                                                  <  1:528460/149‑1 (MQ=255)
                                                aTCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCttt                                                                                                 >  1:1181827/1‑149 (MQ=255)
                                                     gCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGtttt                                                                                            <  2:3403993/149‑1 (MQ=255)
                                                      ccGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGttttt                                                                                           <  1:124797/149‑1 (MQ=255)
                                                       cGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTa                                                                                          <  2:3301460/149‑1 (MQ=255)
                                                            aTCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATgtgt                                                                                     <  2:3778304/149‑1 (MQ=255)
                                                            aTCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATgtgt                                                                                     <  2:372335/149‑1 (MQ=255)
                                                            aTCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATgtgt                                                                                     <  2:595690/149‑1 (MQ=255)
                                                                  ccGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACaa                                                                               <  2:1130092/149‑1 (MQ=255)
                                                                  ccGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACaa                                                                               <  2:301997/149‑1 (MQ=255)
                                                                  ccGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACaa                                                                               <  1:2530545/149‑1 (MQ=255)
                                                                    gACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAAt                                                                             <  1:1751100/149‑1 (MQ=255)
                                                                    gACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAAt                                                                             <  1:3044944/149‑1 (MQ=255)
                                                                     aCAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATc                                                                            >  2:1424544/1‑149 (MQ=255)
                                                                     aCAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATc                                                                            <  1:800101/149‑1 (MQ=255)
                                                                      cAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCa                                                                           <  1:1302502/149‑1 (MQ=255)
                                                                        aCGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCCTTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGa                                                                         <  2:3077951/149‑1 (MQ=255)
                                                                        aCGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGa                                                                         >  2:712645/1‑149 (MQ=255)
                                                                        aCGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGa                                                                         >  1:3701117/1‑149 (MQ=255)
                                                                             gACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGtt                                                                    >  1:3795123/1‑149 (MQ=255)
                                                                             gACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGtt                                                                    >  1:2302759/1‑149 (MQ=255)
                                                                              aCTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTc                                                                   <  2:2616233/149‑1 (MQ=255)
                                                                               cTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCt                                                                  <  1:3523086/149‑1 (MQ=255)
                                                                                 ttAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCttt                                                                >  1:85106/1‑149 (MQ=255)
                                                                                  tAATATCGTCAGCCAGAGCGGTGTGTGAAATGGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCtttt                                                               <  2:1080980/149‑1 (MQ=255)
                                                                                     tatCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCttttttt                                                            <  1:1629077/149‑1 (MQ=255)
                                                                                      atCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTta                                                           <  1:3071943/149‑1 (MQ=255)
                                                                                       tCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTtat                                                          <  2:192128/149‑1 (MQ=255)
                                                                                         gTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATAc                                                        <  1:1774408/149‑1 (MQ=255)
                                                                                         gTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATAc                                                        <  1:2064859/149‑1 (MQ=255)
                                                                                              ccATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCAc                                                   <  2:1891800/149‑1 (MQ=255)
                                                                                              ccATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCAc                                                   <  2:764138/149‑1 (MQ=255)
                                                                                              ccATAGCGGTGTGTGAAAGTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCAc                                                   <  2:1955391/149‑1 (MQ=255)
                                                                                                   gCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGttt                                              <  1:3195003/149‑1 (MQ=255)
                                                                                                   gCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGttt                                              <  1:1141588/149‑1 (MQ=255)
                                                                                                      gtgtgtGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTg                                           >  1:3840747/1‑149 (MQ=255)
                                                                                                        gtgtgAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGcc                                         <  2:2454431/149‑1 (MQ=255)
                                                                                                         tgtgAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCt                                        <  1:2001997/149‑1 (MQ=255)
                                                                                                             aaaTTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCt                                    >  1:1601217/1‑149 (MQ=255)
                                                                                                              aaTTGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTg                                   >  1:1707036/1‑149 (MQ=255)
                                                                                                                 tGCCAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGAtt                                <  1:3614340/149‑1 (MQ=255)
                                                                                                                    cAGTGCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAg                             >  1:554987/1‑149 (MQ=255)
                                                                                                                        gCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGt                         <  2:2500789/149‑1 (MQ=255)
                                                                                                                        gCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGt                         <  2:432992/149‑1 (MQ=255)
                                                                                                                        gCAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGt                         >  2:2242027/1‑149 (MQ=255)
                                                                                                                         cAATCATCCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGtt                        <  1:2514783/149‑1 (MQ=255)
                                                                                                                           atcatcCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAg                      >  1:3232664/1‑149 (MQ=255)
                                                                                                                           atcatcCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAg                      <  1:889762/149‑1 (MQ=255)
                                                                                                                              atcCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAgcgc                   <  2:3797626/149‑1 (MQ=255)
                                                                                                                               tcCCTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCa                  <  1:1485159/149‑1 (MQ=255)
                                                                                                                                cccTGCGATGATAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAg                 <  1:2412173/149‑1 (MQ=255)
                                                                                                                                      gatgatAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTAtttt           <  1:926420/149‑1 (MQ=255)
                                                                                                                                       atgatATTTTTATCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTTTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTATTTtg          <  1:2184055/149‑1 (MQ=255)
                                                                                                                                       atgatAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTATTTtg          >  2:1485248/1‑149 (MQ=255)
                                                                                                                                        tgatAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTATTTtgt         <  1:4105013/149‑1 (MQ=255)
                                                                                                                                         gatAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTATTTtgtg        >  2:2553696/1‑149 (MQ=255)
                                                                                                                                           tAGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTATTTTGTGat      <  2:2939806/149‑1 (MQ=255)
                                                                                                                                            aGTTTTAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTATTTTGTGata     <  2:3176640/149‑1 (MQ=255)
                                                                                                                                               tttAGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATATTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTATTTTGTGataata  <  2:1912295/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTAATTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTATTTTGTGATAATA  >  CP009273/3590760‑3591051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑