Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,596,266 C→A 100% T446T (ACG→ACT)  ftsY ← Signal Recognition Particle (SRP) receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,596,2660CA99.2% 860.5 / ‑2.0 262T446T (ACG→ACT) ftsYSignal Recognition Particle (SRP) receptor
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (136/124);  total (138/124)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 262 total aligned reads displayed.

GGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCT  >  CP009273/3596121‑3596411
                                                                                                                                                 |                                                                                                                                                 
gggCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGtt                                                                                                                                                <  1:3799225/149‑1 (MQ=255)
gggCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGtt                                                                                                                                                >  2:3221401/1‑149 (MQ=255)
 ggCAAAAAGTGCCTCTATAAATTCGTCCGCCTTAAACGGACGCAAATCATCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCAAGTTTAGTTa                                                                                                                                               >  2:707660/1‑149 (MQ=255)
 ggCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTa                                                                                                                                               >  1:3384348/1‑149 (MQ=255)
    aaaaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCg                                                                                                                                            >  1:2761774/1‑149 (MQ=255)
      aaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTg                                                                                                                                          >  2:1870453/1‑149 (MQ=255)
      aaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTg                                                                                                                                          >  1:1865646/1‑149 (MQ=255)
       aaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGa                                                                                                                                         <  1:4101287/149‑1 (MQ=255)
          tGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGc                                                                                                                                      <  2:2827948/149‑1 (MQ=255)
          tGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGc                                                                                                                                      <  2:141772/149‑1 (MQ=255)
           gCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGcc                                                                                                                                     >  1:3239797/1‑149 (MQ=255)
           gCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGcc                                                                                                                                     >  1:577866/1‑149 (MQ=255)
             ctctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCgg                                                                                                                                   >  2:288466/1‑149 (MQ=255)
             ctctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCgg                                                                                                                                   <  1:431899/149‑1 (MQ=255)
             ctctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCgg                                                                                                                                   >  1:1139115/1‑149 (MQ=255)
             atctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTACGCCGACACCAAAGTAGCGGATAGGGATACCAAACTGTTAATCCACAGAGAAAATTACACCGCCTTTATCCCAGCCGTCCAGTTTAGTAAGAGAGATGCCgg                                                                                                                                   >  2:431066/2‑149 (MQ=255)
               ctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGtt                                                                                                                                 >  2:3458891/1‑149 (MQ=255)
                tataAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTa                                                                                                                                <  1:1535818/149‑1 (MQ=255)
                tataAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTa                                                                                                                                <  1:1346486/149‑1 (MQ=255)
                  taAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAg                                                                                                                              >  1:3111743/1‑149 (MQ=255)
                  taAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAg                                                                                                                              >  1:3111547/1‑149 (MQ=255)
                   aaaGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGc                                                                                                                             <  1:1601096/149‑1 (MQ=255)
                   aaaGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGc                                                                                                                             <  2:644864/149‑1 (MQ=255)
                   aaaGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGc                                                                                                                             <  1:1255040/149‑1 (MQ=255)
                     aGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCa                                                                                                                           >  2:3095032/1‑149 (MQ=255)
                      gtcgtcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCaa                                                                                                                          >  1:2542451/1‑149 (MQ=255)
                        cgtcCGCCTTAAACTGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACg                                                                                                                        <  2:2905/149‑1 (MQ=255)
                         gtcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACgg                                                                                                                       >  2:2238745/1‑149 (MQ=255)
                          tcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGc                                                                                                                      >  2:2138779/1‑149 (MQ=255)
                               cTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCAt                                                                                                                 >  1:2337361/1‑149 (MQ=255)
                                   aaCGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGaa                                                                                                             <  2:3515018/149‑1 (MQ=255)
                                   aaCGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGaa                                                                                                             >  1:951579/1‑149 (MQ=255)
                                   aaCGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGaa                                                                                                             >  1:2243861/1‑149 (MQ=255)
                                     cGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACa                                                                                                           <  2:2343561/149‑1 (MQ=255)
                                     cGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACa                                                                                                           <  2:270420/149‑1 (MQ=255)
                                        aCGCAAATCCTCAATACGTTCGCCGACACCAATGTATCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGATTCATGGAACAGtt                                                                                                        >  1:3054486/1‑149 (MQ=255)
                                        aCGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGtt                                                                                                        <  1:526915/149‑1 (MQ=255)
                                           cAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTgg                                                                                                     <  2:155284/149‑1 (MQ=255)
                                             aaTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGcc                                                                                                   >  2:424702/1‑149 (MQ=255)
                                             aaTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGcc                                                                                                   <  1:1042691/149‑1 (MQ=255)
                                             aaTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTATCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGcc                                                                                                   >  2:2440807/1‑149 (MQ=255)
                                              aTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCt                                                                                                  >  1:604718/1‑149 (MQ=255)
                                              aTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCt                                                                                                  <  1:178166/149‑1 (MQ=255)
                                               tCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTg                                                                                                 >  2:41564/1‑149 (MQ=255)
                                                  tCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCt                                                                                              >  1:876130/1‑149 (MQ=255)
                                                   cAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCtt                                                                                             >  1:1425757/1‑149 (MQ=255)
                                                    aaTACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTa                                                                                            <  1:2151946/149‑1 (MQ=255)
                                                    aaTACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTa                                                                                            >  2:1738758/1‑149 (MQ=255)
                                                      tACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTAcc                                                                                          >  1:3533255/1‑149 (MQ=255)
                                                      tACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTAcc                                                                                          >  1:1004380/1‑149 (MQ=255)
                                                            cGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTc                                                                                    <  2:1547720/149‑1 (MQ=255)
                                                             gCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAAAAGTTTGGCCTGGCTTACCGCGTTCt                                                                                   >  2:3939862/1‑149 (MQ=255)
                                                                 acacCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGccc                                                                               <  2:3103399/149‑1 (MQ=255)
                                                                 acacCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGccc                                                                               <  1:3208717/149‑1 (MQ=255)
                                                                   acCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCgg                                                                             >  2:3589904/1‑149 (MQ=255)
                                                                   acCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCgg                                                                             <  1:729184/149‑1 (MQ=255)
                                                                      aaTGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGc                                                                          >  2:4092273/1‑149 (MQ=255)
                                                                       aTGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCt                                                                         <  2:1254937/149‑1 (MQ=255)
                                                                       aTGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCt                                                                         <  2:2212502/149‑1 (MQ=255)
                                                                       aTGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCt                                                                         >  2:4050089/1‑149 (MQ=255)
                                                                       aTGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTATGGCCTGGCTTACCGCGTTCTGCCCGGTGCt                                                                         >  2:4011289/1‑149 (MQ=255)
                                                                         gTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTgg                                                                       <  1:1579577/149‑1 (MQ=255)
                                                                                aTAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAt                                                                >  1:1924334/1‑149 (MQ=255)
                                                                                aTAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAt                                                                <  1:3624877/149‑1 (MQ=255)
                                                                                 tAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATa                                                               >  1:2939944/1‑149 (MQ=255)
                                                                                  aGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAg                                                              <  2:1456833/149‑1 (MQ=255)
                                                                                     gATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCa                                                           <  1:1660458/149‑1 (MQ=255)
                                                                                        aCCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCa                                                        <  1:1189884/149‑1 (MQ=255)
                                                                                           aaaCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATaa                                                     >  1:2056057/1‑149 (MQ=255)
                                                                                           aaaCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATaa                                                     <  2:2669323/149‑1 (MQ=255)
                                                                                            aaCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAAc                                                    >  1:3348577/1‑149 (MQ=255)
                                                                                             aCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACt                                                   >  2:2509966/1‑149 (MQ=255)
                                                                                             aCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACt                                                   <  2:1991115/149‑1 (MQ=255)
                                                                                                     gCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATgcgg                                           >  2:1523407/1‑149 (MQ=255)
                                                                                                        aCCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGgcgc                                        <  2:1399702/149‑1 (MQ=255)
                                                                                                           gagaAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTc                                     <  1:1484332/149‑1 (MQ=255)
                                                                                                              aaaaTTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAAc                                  >  2:591761/1‑149 (MQ=255)
                                                                                                                aaTTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGt                                >  2:1141929/1‑149 (MQ=255)
                                                                                                                 aTTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTc                               <  2:490304/149‑1 (MQ=255)
                                                                                                                      cccGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGtt                          <  1:2260325/149‑1 (MQ=255)
                                                                                                                       ccGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGttt                         >  2:2618048/1‑149 (MQ=255)
                                                                                                                        cGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGCGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttc                        <  2:1134984/149‑1 (MQ=255)
                                                                                                                           cTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttcttc                     >  1:2624148/1‑149 (MQ=255)
                                                                                                                               cGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcatca                 <  2:1814223/149‑1 (MQ=255)
                                                                                                                                 ccgtgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcg               >  1:3037712/1‑149 (MQ=255)
                                                                                                                                  cgtgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgc              >  1:2780410/1‑149 (MQ=255)
                                                                                                                                   gtgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCCTAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgcg             >  2:3945165/1‑149 (MQ=255)
                                                                                                                                   gtgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgcg             >  2:3533096/1‑149 (MQ=255)
                                                                                                                                     gccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGa           <  2:829888/149‑1 (MQ=255)
                                                                                                                                      ccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGAc          >  2:944402/1‑149 (MQ=255)
                                                                                                                                         tCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt       <  1:2532944/149‑1 (MQ=255)
                                                                                                                                         tCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt       >  2:743437/1‑149 (MQ=255)
                                                                                                                                         tCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt       >  2:2996050/1‑149 (MQ=255)
                                                                                                                                           cAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAttt     <  1:3759224/149‑1 (MQ=255)
                                                                                                                                           cAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAttt     <  1:726255/149‑1 (MQ=255)
                                                                                                                                           cAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAttt     >  2:1476208/1‑149 (MQ=255)
                                                                                                                                            aGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAtttt    >  2:3264891/1‑149 (MQ=255)
                                                                                                                                              tttCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttct  >  2:1912640/1‑149 (MQ=255)
                                                                                                                                              tttAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttct  <  2:681101/149‑1 (MQ=255)
                                                                                                                                              tttAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttct  >  2:3120903/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCT  >  CP009273/3596121‑3596411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑