Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,607,110 C→A 100% I28I (ATC→ATA)  nikA → nickel‑binding, heme‑binding periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,607,1100CA97.2% 796.6 / 10.7 252I28I (ATC→ATA) nikAnickel‑binding, heme‑binding periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base A (127/118);  total (129/123)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 259 total aligned reads displayed.

ATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGA  >  CP009273/3606964‑3607255
                                                                                                                                                  |                                                                                                                                                 
 tGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACANTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaacc                                                                                                                                                >  1:2086041/1‑149 (MQ=255)
       aTACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                          <  2:961978/149‑1 (MQ=255)
       aTACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                          <  2:3951917/149‑1 (MQ=255)
       aTACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                          <  2:3563162/149‑1 (MQ=255)
       aTACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                          <  2:1914004/149‑1 (MQ=255)
       aTACTTAAAATCGTCATACTTATTCCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                          >  2:884704/1‑149 (MQ=255)
         aCTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTg                                                                                                                                        >  2:3043173/1‑149 (MQ=255)
           ttAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGc                                                                                                                                      >  2:3625682/1‑149 (MQ=255)
              aaaTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCgg                                                                                                                                   >  2:545546/1‑149 (MQ=255)
              aaaTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCgg                                                                                                                                   <  2:2965374/149‑1 (MQ=255)
              aaaTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCgg                                                                                                                                   >  2:2698619/1‑149 (MQ=255)
                 tCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGa                                                                                                                                >  1:1079841/1‑149 (MQ=255)
                   gTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAAt                                                                                                                              >  1:1120396/1‑149 (MQ=255)
                     cATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGt                                                                                                                            >  1:2056348/1‑149 (MQ=255)
                     cATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGt                                                                                                                            <  2:2407461/149‑1 (MQ=255)
                     cATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGt                                                                                                                            <  1:2697912/149‑1 (MQ=255)
                      aTACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTc                                                                                                                           <  2:3906535/149‑1 (MQ=255)
                      aTACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTc                                                                                                                           >  2:388396/1‑149 (MQ=255)
                       tACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCg                                                                                                                          >  2:2881328/1‑149 (MQ=255)
                        aCTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCgg                                                                                                                         >  1:1042089/1‑149 (MQ=255)
                         cTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCggg                                                                                                                        >  1:1316428/1‑149 (MQ=255)
                         cTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCggg                                                                                                                        >  1:3157484/1‑149 (MQ=255)
                          ttATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGc                                                                                                                       <  2:587587/149‑1 (MQ=255)
                                ccGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTaa                                                                                                                 >  2:3691011/1‑149 (MQ=255)
                                ccGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTaa                                                                                                                 <  2:2914120/149‑1 (MQ=255)
                                  gCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATTCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAc                                                                                                               >  1:664590/1‑149 (MQ=255)
                                  gCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAc                                                                                                               <  1:1988924/149‑1 (MQ=255)
                                   ccATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCAGGTGAATGTCGGGCCACTAAAcc                                                                                                              >  1:894402/1‑149 (MQ=255)
                                    cATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAccc                                                                                                             >  1:3915515/1‑149 (MQ=255)
                                     aTCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCg                                                                                                            >  2:1006177/1‑149 (MQ=255)
                                     aTCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCg                                                                                                            >  1:112962/1‑149 (MQ=255)
                                      tCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGc                                                                                                           >  1:1619739/1‑149 (MQ=255)
                                       cTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCa                                                                                                          >  2:3406811/1‑149 (MQ=255)
                                        tATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCAc                                                                                                         >  2:1742919/1‑149 (MQ=255)
                                        tATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCAc                                                                                                         >  2:1741921/1‑149 (MQ=255)
                                        tATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCAc                                                                                                         >  2:1153853/1‑149 (MQ=255)
                                           tttAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCtt                                                                                                      <  1:939300/149‑1 (MQ=255)
                                           tttAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCtt                                                                                                      >  2:637940/1‑149 (MQ=255)
                                            ttAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCttt                                                                                                     <  2:1620448/149‑1 (MQ=255)
                                             tAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTa                                                                                                    <  1:3092871/149‑1 (MQ=255)
                                               aTCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCTCCTTTaca                                                                                                  >  2:211938/1‑149 (MQ=255)
                                               aTCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTaca                                                                                                  <  2:2343866/149‑1 (MQ=255)
                                               aTCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTaca                                                                                                  >  1:249404/1‑149 (MQ=255)
                                                tCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTacac                                                                                                 <  1:402621/149‑1 (MQ=255)
                                                tCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTacac                                                                                                 <  1:1744013/149‑1 (MQ=255)
                                                  cATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGc                                                                                               <  1:1166798/149‑1 (MQ=255)
                                                  cATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGc                                                                                               >  1:2718553/1‑149 (MQ=255)
                                                   aTTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGcc                                                                                              >  1:4076400/1‑149 (MQ=255)
                                                            aCCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGAt                                                                                     >  2:75582/1‑149 (MQ=255)
                                                               aTGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGtt                                                                                  <  2:2361099/149‑1 (MQ=255)
                                                               aTGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGtt                                                                                  >  1:3516/1‑149 (MQ=255)
                                                                 gCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCg                                                                                >  1:3399438/1‑149 (MQ=255)
                                                                    ctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGccc                                                                             <  2:1295097/149‑1 (MQ=255)
                                                                      ccACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCag                                                                           >  1:1799614/1‑149 (MQ=255)
                                                                       cacaCTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCaga                                                                          >  1:435294/1‑149 (MQ=255)
                                                                        acacTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCagag                                                                         >  1:3310489/1‑149 (MQ=255)
                                                                          acTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCa                                                                       >  1:3362494/1‑149 (MQ=255)
                                                                           cTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCAt                                                                      <  1:3151727/149‑1 (MQ=255)
                                                                           cTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCAt                                                                      <  1:3702286/149‑1 (MQ=255)
                                                                             ccgccgCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATgg                                                                    <  1:293541/149‑1 (MQ=255)
                                                                             ccgccgCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATgg                                                                    <  1:73778/149‑1 (MQ=255)
                                                                              cgccgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGt                                                                   <  2:1350358/149‑1 (MQ=255)
                                                                               gccgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGtt                                                                  >  1:249926/1‑149 (MQ=255)
                                                                               gccgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGtt                                                                  <  2:1607292/149‑1 (MQ=255)
                                                                                  gcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTAt                                                               >  1:2689126/1‑149 (MQ=255)
                                                                                  gcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTAt                                                               >  1:1577586/1‑149 (MQ=255)
                                                                                      tctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAAc                                                           <  1:3216586/149‑1 (MQ=255)
                                                                                        tATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCa                                                         >  2:2551913/1‑149 (MQ=255)
                                                                                        tATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCa                                                         <  2:3637767/149‑1 (MQ=255)
                                                                                         aTTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCAt                                                        >  2:439389/1‑149 (MQ=255)
                                                                                           ttGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTAGACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTg                                                      <  1:1741921/149‑1 (MQ=255)
                                                                                           ttGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTg                                                      <  1:2559719/149‑1 (MQ=255)
                                                                                            tGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTgg                                                     <  2:435294/149‑1 (MQ=255)
                                                                                              cgcTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTg                                                   <  2:190233/149‑1 (MQ=255)
                                                                                               gctgctGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGa                                                  <  2:3152773/149‑1 (MQ=255)
                                                                                                ctgctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGaa                                                 <  1:2617216/149‑1 (MQ=255)
                                                                                                   ctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCAGTGGTGAAata                                              >  2:4029548/1‑149 (MQ=255)
                                                                                                     ggCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATc                                            >  2:1042389/1‑149 (MQ=255)
                                                                                                     ggCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATc                                            >  1:2292488/1‑149 (MQ=255)
                                                                                                        ttGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAgg                                         <  2:2266310/149‑1 (MQ=255)
                                                                                                           tgCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAg                                      <  2:3461546/149‑1 (MQ=255)
                                                                                                             cGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGAc                                    <  1:3004375/149‑1 (MQ=255)
                                                                                                             cGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGAc                                    <  1:2238136/149‑1 (MQ=255)
                                                                                                              gTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACg                                   >  1:3700437/1‑149 (MQ=255)
                                                                                                                 ttttATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGtt                                <  2:3507196/149‑1 (MQ=255)
                                                                                                                   ttATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCg                              >  2:3733460/1‑149 (MQ=255)
                                                                                                                     aTCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGt                            >  1:868346/1‑149 (MQ=255)
                                                                                                                       cGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGa                          <  2:2032036/149‑1 (MQ=255)
                                                                                                                        gTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGAt                         <  1:2656536/149‑1 (MQ=255)
                                                                                                                        gTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGAt                         <  1:2659793/149‑1 (MQ=255)
                                                                                                                        gTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGAt                         <  1:591136/149‑1 (MQ=255)
                                                                                                                          ccATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATcc                       <  1:2359149/149‑1 (MQ=255)
                                                                                                                             tGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGt                    <  2:405126/149‑1 (MQ=255)
                                                                                                                                 gCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggct                >  1:3117042/1‑149 (MQ=255)
                                                                                                                                    gCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggctggc             <  2:2184537/149‑1 (MQ=255)
                                                                                                                                      aCCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaa           >  1:3079508/1‑149 (MQ=255)
                                                                                                                                       ccAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGCGCCACTAAACCCGCACCTTTACACGCCAAACCAGATGTACGCCCAGAGCATGGTTAATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaaa          <  2:308534/149‑1 (MQ=255)
                                                                                                                                           aTGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGc      >  1:509671/1‑149 (MQ=255)
                                                                                                                                            tGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCt     <  2:3997336/149‑1 (MQ=255)
                                                                                                                                               aaTAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGa  >  2:3360943/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGA  >  CP009273/3606964‑3607255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑