Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,622,058 C→A 100% D281Y (GAT→TAT)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,622,0580CA96.4% 670.9 / 14.0 220D281Y (GAT→TAT) rbbAribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/6);  new base A (89/123);  total (91/129)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 228 total aligned reads displayed.

GCTGGCGGGCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCG  >  CP009273/3621912‑3622203
                                                                                                                                                  |                                                                                                                                                 
gCTGGCGGGCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATagc                                                                                                                                                 <  1:2953028/149‑1 (MQ=255)
  tGGCGGGCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATagcgc                                                                                                                                               <  1:2433289/149‑1 (MQ=255)
  tGGCGGGCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATagcgc                                                                                                                                               >  2:3725454/1‑149 (MQ=255)
  tGGCGGGCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATagcgc                                                                                                                                               >  2:1680971/1‑149 (MQ=255)
   ggCGGGCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATagcgcg                                                                                                                                              >  1:3533270/1‑149 (MQ=255)
   ggCGGGCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATagcgcg                                                                                                                                              >  1:1580469/1‑149 (MQ=255)
     cGGGCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCt                                                                                                                                            <  1:178395/149‑1 (MQ=255)
      gggCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCtt                                                                                                                                           <  1:1026578/149‑1 (MQ=255)
      gggCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCtt                                                                                                                                           >  1:1985590/1‑149 (MQ=255)
      gggCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCtt                                                                                                                                           >  1:192185/1‑149 (MQ=255)
       ggCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTc                                                                                                                                          <  2:1574607/149‑1 (MQ=255)
       ggCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTc                                                                                                                                          <  2:3673040/149‑1 (MQ=255)
          agcagTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGAt                                                                                                                                       >  1:3191369/1‑149 (MQ=255)
          agcagTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGAt                                                                                                                                       <  1:2006591/149‑1 (MQ=255)
           gcagTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATg                                                                                                                                      >  2:1684943/1‑149 (MQ=255)
             agTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGc                                                                                                                                    >  1:2624833/1‑149 (MQ=255)
             agTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGc                                                                                                                                    >  1:1095422/1‑149 (MQ=255)
              gTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCa                                                                                                                                   >  1:1672852/1‑149 (MQ=255)
                ccGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAAt                                                                                                                                 >  1:3401886/1‑149 (MQ=255)
                 cGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAAtc                                                                                                                                <  2:1075899/149‑1 (MQ=255)
                   gTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAAtctc                                                                                                                              <  1:3248822/149‑1 (MQ=255)
                   gTGAGCATTTTCATCGTGGTGGATTTACCCCAGCCGGTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAAtctc                                                                                                                              <  1:3120358/149‑1 (MQ=255)
                    tGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATctct                                                                                                                             >  2:832019/1‑149 (MQ=255)
                        cATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGt                                                                                                                         <  1:2125019/149‑1 (MQ=255)
                            ttCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTc                                                                                                                     <  1:1807525/149‑1 (MQ=255)
                                 ggtggtggATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGtt                                                                                                                <  2:121548/149‑1 (MQ=255)
                                        gATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACgg                                                                                                         >  2:3816231/1‑149 (MQ=255)
                                         aTTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGt                                                                                                        >  2:782721/1‑149 (MQ=255)
                                         aTTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGt                                                                                                        <  2:2711827/149‑1 (MQ=255)
                                                      gTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCg                                                                                           >  2:3624175/1‑149 (MQ=255)
                                                       ttCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGc                                                                                          >  1:2199706/1‑149 (MQ=255)
                                                       ttCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGc                                                                                          >  1:124121/1‑149 (MQ=255)
                                                       ttCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGc                                                                                          >  2:1003992/1‑149 (MQ=255)
                                                         cGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCt                                                                                        <  1:3708378/149‑1 (MQ=255)
                                                              cAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATgc                                                                                   <  2:1572588/149‑1 (MQ=255)
                                                                 gAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGcc                                                                                <  1:1085534/149‑1 (MQ=255)
                                                                 gAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGcc                                                                                <  2:3164304/149‑1 (MQ=255)
                                                                  aaaaCCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCt                                                                               >  1:3934281/1‑149 (MQ=255)
                                                                   aaaCCAAAGATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTg                                                                              >  1:759099/1‑149 (MQ=255)
                                                                   aaaCCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTg                                                                              <  1:611227/149‑1 (MQ=255)
                                                                   aaaCCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGAGAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTg                                                                              >  2:1293755/1‑149 (MQ=255)
                                                                     aCCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGc                                                                            <  2:1693238/149‑1 (MQ=255)
                                                                      ccAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCg                                                                           >  1:1632365/1‑149 (MQ=255)
                                                                       cAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGt                                                                          <  2:2730962/149‑1 (MQ=255)
                                                                        aaaaaTCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGtt                                                                         <  2:2951908/149‑1 (MQ=255)
                                                                         aaaaTCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACTAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTg                                                                        <  2:761939/149‑1 (MQ=255)
                                                                         aaaaTCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTg                                                                        <  1:1429053/149‑1 (MQ=255)
                                                                            aTCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTgcgc                                                                     >  2:2956467/1‑149 (MQ=255)
                                                                            aTCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTgcgc                                                                     <  2:3554520/149‑1 (MQ=255)
                                                                             tctcCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCt                                                                    <  2:3401886/149‑1 (MQ=255)
                                                                             tctcCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCt                                                                    <  1:869605/149‑1 (MQ=255)
                                                                             tctcCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCt                                                                    <  2:1351875/149‑1 (MQ=255)
                                                                                 cccGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCg                                                                >  2:3047096/1‑149 (MQ=255)
                                                                                 cccGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCg                                                                >  1:1306165/1‑149 (MQ=255)
                                                                                   cgcgTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGt                                                              <  1:3585474/149‑1 (MQ=255)
                                                                                    gcgTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTa                                                             <  1:641767/149‑1 (MQ=255)
                                                                                     cgTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTaa                                                            <  2:1985590/149‑1 (MQ=255)
                                                                                     cgTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTaa                                                            <  2:192185/149‑1 (MQ=255)
                                                                                         gAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGa                                                        <  1:1735650/149‑1 (MQ=255)
                                                                                         gAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGa                                                        <  2:668401/149‑1 (MQ=255)
                                                                                            tGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGAttt                                                     >  2:3088390/1‑149 (MQ=255)
                                                                                            tGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGAttt                                                     >  2:1194060/1‑149 (MQ=255)
                                                                                            tGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGAttt                                                     >  1:1956202/1‑149 (MQ=255)
                                                                                             gCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTta                                                    <  2:3582550/149‑1 (MQ=255)
                                                                                             gCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTta                                                    <  2:1537350/149‑1 (MQ=255)
                                                                                              cGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTtat                                                   <  1:1267245/149‑1 (MQ=255)
                                                                                               ggAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTtata                                                  <  2:206944/149‑1 (MQ=255)
                                                                                               ggAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTtata                                                  <  2:115765/149‑1 (MQ=255)
                                                                                                  aaTTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAAt                                               <  1:951624/149‑1 (MQ=255)
                                                                                                  aaTTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAAt                                               >  1:1792352/1‑149 (MQ=255)
                                                                                                   aTTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATg                                              <  1:2583494/149‑1 (MQ=255)
                                                                                                    ttAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGc                                             >  2:475655/1‑149 (MQ=255)
                                                                                                      aaCGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGctt                                           <  1:3558731/149‑1 (MQ=255)
                                                                                                       aCGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGcttc                                          >  2:2217527/1‑149 (MQ=255)
                                                                                                       aCGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGcttc                                          >  2:2856099/1‑149 (MQ=255)
                                                                                                       aCGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGcttc                                          <  1:2859972/149‑1 (MQ=255)
                                                                                                         gTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGcttctt                                        <  1:2048744/149‑1 (MQ=255)
                                                                                                         gTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGcttctt                                        >  2:1714111/1‑149 (MQ=255)
                                                                                                           gATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTcc                                      >  2:94139/1‑149 (MQ=255)
                                                                                                           gATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTcc                                      <  1:2646922/149‑1 (MQ=255)
                                                                                                           gATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTcc                                      <  1:286517/149‑1 (MQ=255)
                                                                                                           gATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTcc                                      <  1:96956/149‑1 (MQ=255)
                                                                                                            aTCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCa                                     <  1:2312792/149‑1 (MQ=255)
                                                                                                                 cGGCAACGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTa                                >  1:756978/1‑149 (MQ=255)
                                                                                                                      aCGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCt                           <  1:1173648/149‑1 (MQ=255)
                                                                                                                      aCGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCt                           <  1:409199/149‑1 (MQ=255)
                                                                                                                      aCGAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCt                           <  2:215626/149‑1 (MQ=255)
                                                                                                                       cGATGGAACCAACACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCtt                          <  2:25782/149‑1 (MQ=255)
                                                                                                                        gAAGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCttt                         >  1:39549/1‑149 (MQ=255)
                                                                                                                          aGGAACCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGc                       <  2:3990034/149‑1 (MQ=255)
                                                                                                                              aCCAAACCGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGttt                   <  2:2309222/149‑1 (MQ=255)
                                                                                                                              aCCAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGttt                   <  2:817753/149‑1 (MQ=255)
                                                                                                                               ccAAAACGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTtg                  >  2:2447068/1‑149 (MQ=255)
                                                                                                                                 aaaaCGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTtgct                <  2:3191369/149‑1 (MQ=255)
                                                                                                                                    aCGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGcc             <  1:3725454/149‑1 (MQ=255)
                                                                                                                                     cGCATGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCg            <  2:3286156/149‑1 (MQ=255)
                                                                                                                                         tGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTACCAGATTTATCAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGc        >  2:3277565/1‑149 (MQ=255)
                                                                                                                                         tGGTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGc        <  1:1564578/149‑1 (MQ=255)
                                                                                                                                           gTCAGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGctc      <  1:1001197/149‑1 (MQ=255)
                                                                                                                                              aGATAGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTc   <  2:2624833/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCTGGCGGGCAGCAGTCCGGTGAGCATTTTCATGGTGGTGGATTTACCGCAGCCGTTCGAACCAAGAAAACCAAAAATCTCCCCGCGTGGAATGCGGAAATTAACGTGATCAACGGCAACGAAGGAACCAAAACGCATGGTCAGATCGCGCGCTTCGATGGCAATCTCTGCGTTTTCAGGTTGATACGGTGGGATCACTACCGCCTGATGCGCCTGGCGTTGCGCTTGCGGTAACAGATTTATAAATGCTTCTTCCAGCGTAGCGCTTTGCGTTTGCTGCCGTAGCTCTTCG  >  CP009273/3621912‑3622203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑