Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,639,239 G→T 93.7% P165P (CCG→CCT)  rlmJ → 23S rRNA m(6)A2030 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,639,2390GT93.7% 675.8 / 35.3 238P165P (CCG→CCT) rlmJ23S rRNA m(6)A2030 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (10/5);  new base T (91/132);  total (101/137)
Fisher's exact test for biased strand distribution p-value = 6.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 239 total aligned reads displayed.

CCGAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGC  >  CP009273/3639094‑3639382
                                                                                                                                                 |                                                                                                                                               
   aaCTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTat                                                                                                                                           >  1:3994222/1‑149 (MQ=255)
   aaCTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTat                                                                                                                                           <  2:1975982/149‑1 (MQ=255)
   aaCTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTat                                                                                                                                           >  2:169094/1‑149 (MQ=255)
   aaCTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTat                                                                                                                                           >  2:2800974/1‑149 (MQ=255)
    aCTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatg                                                                                                                                          >  1:1949328/1‑149 (MQ=255)
        cACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaaat                                                                                                                                      >  1:2523414/1‑149 (MQ=255)
          cccGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaaatga                                                                                                                                    >  1:2582688/1‑149 (MQ=255)
              aGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAAc                                                                                                                                <  1:621747/149‑1 (MQ=255)
              aGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAAc                                                                                                                                >  2:208255/1‑149 (MQ=255)
              aGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAAc                                                                                                                                >  2:208357/1‑149 (MQ=255)
              aGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAAc                                                                                                                                <  1:989736/149‑1 (MQ=255)
               gCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACt                                                                                                                               >  1:1379281/1‑149 (MQ=255)
                  aTTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGAc                                                                                                                            <  1:1022615/149‑1 (MQ=255)
                      cccGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATc                                                                                                                        <  2:3065050/149‑1 (MQ=255)
                      cccGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATc                                                                                                                        <  1:2117703/149‑1 (MQ=255)
                      cccGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATc                                                                                                                        <  2:3627445/149‑1 (MQ=255)
                       ccGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCa                                                                                                                       >  2:4026156/1‑149 (MQ=255)
                        cGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCaa                                                                                                                      <  2:1724531/149‑1 (MQ=255)
                        cGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCaa                                                                                                                      <  2:1669317/149‑1 (MQ=255)
                        cGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCaa                                                                                                                      <  2:2510861/149‑1 (MQ=255)
                         gttgttGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCTTGTCGAAAAAGGCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAg                                                                                                                     <  1:846703/149‑1 (MQ=255)
                           tgttgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCGTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCg                                                                                                                   >  1:3489557/1‑149 (MQ=255)
                               gCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTAACTATCAAGCggtgg                                                                                                               >  1:313662/1‑149 (MQ=255)
                                  ttCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCa                                                                                                            <  2:3462337/149‑1 (MQ=255)
                                     tGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCg                                                                                                         <  1:1095272/149‑1 (MQ=255)
                                      gAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCgg                                                                                                        <  1:2997956/149‑1 (MQ=255)
                                       aaTTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCggg                                                                                                       >  2:1738438/1‑149 (MQ=255)
                                         tttCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGAt                                                                                                     <  1:2145745/149‑1 (MQ=255)
                                           tCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAg                                                                                                   >  1:3299542/1‑149 (MQ=255)
                                              gAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAg                                                                                                >  1:107012/1‑149 (MQ=255)
                                              gAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAg                                                                                                >  1:2779172/1‑149 (MQ=255)
                                              gAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGGGGTCAGCGGGATAGCAg                                                                                                >  1:493073/1‑149 (MQ=255)
                                               aaaGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGa                                                                                               <  1:407554/149‑1 (MQ=255)
                                                 aGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAg                                                                                             <  1:856446/149‑1 (MQ=255)
                                                         gttcgcgTGTAGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCTAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCTTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACaaa                                                                                     <  2:7989/146‑1 (MQ=255)
                                                         gTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACaaa                                                                                     <  2:2149145/149‑1 (MQ=255)
                                                         gTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACaaa                                                                                     <  2:2370892/149‑1 (MQ=255)
                                                                  tCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGcc                                                                            <  2:1651928/149‑1 (MQ=255)
                                                                     aaaaaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACt                                                                         >  1:2753120/1‑149 (MQ=255)
                                                                     aaaaaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACt                                                                         <  2:1172022/149‑1 (MQ=255)
                                                                     aaaaaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACt                                                                         >  2:1388503/1‑149 (MQ=255)
                                                                        aaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGt                                                                      <  2:3221030/149‑1 (MQ=255)
                                                                          gCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                    <  1:3639317/149‑1 (MQ=255)
                                                                          gCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                    >  1:1991329/1‑149 (MQ=255)
                                                                          gCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                    <  1:2675694/149‑1 (MQ=255)
                                                                          gCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAAAGTTTCGCCACTGGTAt                                                                    >  1:3787095/1‑149 (MQ=255)
                                                                            cGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTGATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAttt                                                                  <  2:879901/149‑1 (MQ=255)
                                                                             gACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTa                                                                 >  2:1477368/1‑149 (MQ=255)
                                                                             gACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTa                                                                 >  1:485217/1‑149 (MQ=255)
                                                                              aCGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTAc                                                                >  1:2227519/1‑149 (MQ=255)
                                                                              aCGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTAc                                                                <  2:2016203/149‑1 (MQ=255)
                                                                              aCGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTAc                                                                <  1:151825/149‑1 (MQ=255)
                                                                                ggTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGc                                                              >  1:1291566/1‑149 (MQ=255)
                                                                                 gTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCa                                                             >  2:1309201/1‑149 (MQ=255)
                                                                                 gTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCa                                                             >  1:130669/1‑149 (MQ=255)
                                                                                      cagcagCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtgtg                                                        >  1:1847301/1‑149 (MQ=255)
                                                                                      cagcagCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtgtg                                                        <  1:1682720/149‑1 (MQ=255)
                                                                                       agcagcTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTgg                                                       <  1:898901/149‑1 (MQ=255)
                                                                                          agcTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTAt                                                    <  1:3553487/149‑1 (MQ=255)
                                                                                           gcTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATc                                                   >  1:242669/1‑149 (MQ=255)
                                                                                           gcTTAAGGCCAAACTGCCGCCGGTTTCCCGCAGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCAATGGTATTTACGCACTGTGGTATc                                                   <  2:1918302/149‑1 (MQ=255)
                                                                                             ttAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCg                                                 <  2:1828669/149‑1 (MQ=255)
                                                                                             ttAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCg                                                 <  2:3551103/149‑1 (MQ=255)
                                                                                               aaGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggt                                               <  2:2853111/149‑1 (MQ=255)
                                                                                                aGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtg                                              >  1:549769/1‑149 (MQ=255)
                                                                                                 ggCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtgg                                             <  1:3337409/149‑1 (MQ=255)
                                                                                                 ggCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtgg                                             <  2:3703525/149‑1 (MQ=255)
                                                                                                 ggCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtgg                                             <  1:1285248/149‑1 (MQ=255)
                                                                                                  gCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtggt                                            >  1:2944278/1‑149 (MQ=255)
                                                                                                    cAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgc                                          <  1:3389310/149‑1 (MQ=255)
                                                                                                    cAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgc                                          <  1:742660/149‑1 (MQ=255)
                                                                                                    cAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgc                                          <  2:23197/149‑1 (MQ=255)
                                                                                                     aaaCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgct                                         >  1:2502996/1‑149 (MQ=255)
                                                                                                     aaaCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgct                                         <  1:398440/149‑1 (MQ=255)
                                                                                                      aaCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgctg                                        <  2:3873283/149‑1 (MQ=255)
                                                                                                           ccgccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCa                                   <  2:3320892/149‑1 (MQ=255)
                                                                                                             gccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGc                                 <  2:3000083/149‑1 (MQ=255)
                                                                                                             gccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGc                                 >  2:3934211/1‑149 (MQ=255)
                                                                                                               cgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCaa                               >  1:2233131/1‑149 (MQ=255)
                                                                                                                ggTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCaaa                              <  2:1948517/149‑1 (MQ=255)
                                                                                                                ggTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCaaa                              >  2:1520985/1‑149 (MQ=255)
                                                                                                                       cgccgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAgcg                       >  1:3718200/1‑149 (MQ=255)
                                                                                                                          cgTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCAt                    >  1:1772145/1‑149 (MQ=255)
                                                                                                                           gTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATg                   >  1:3483314/1‑149 (MQ=255)
                                                                                                                            tGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGa                  >  2:3150262/1‑149 (MQ=255)
                                                                                                                            tGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGa                  <  2:862436/149‑1 (MQ=255)
                                                                                                                            tGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGa                  <  2:2582688/149‑1 (MQ=255)
                                                                                                                             ggTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGAt                 <  1:951695/149‑1 (MQ=255)
                                                                                                                              gTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                <  1:2271121/149‑1 (MQ=255)
                                                                                                                               tttAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATcc               <  1:2405507/149‑1 (MQ=255)
                                                                                                                                   aTCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGa           <  1:1898481/149‑1 (MQ=255)
                                                                                                                                   aTCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGa           <  2:510988/149‑1 (MQ=255)
                                                                                                                                   aTCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGa           <  1:3937217/149‑1 (MQ=255)
                                                                                                                                     ccTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATc         <  2:2751271/149‑1 (MQ=255)
                                                                                                                                         aTCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGa     <  1:1181097/149‑1 (MQ=255)
                                                                                                                                         aTCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGa     >  1:452080/1‑149 (MQ=255)
                                                                                                                                            gACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGc  <  2:1130763/149‑1 (MQ=255)
                                                                                                                                            gACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGc  <  2:3688024/149‑1 (MQ=255)
                                                                                                                                            gACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGc  <  2:3270711/149‑1 (MQ=255)
                                                                                                                                            gACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGc  >  1:1541262/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCGAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGC  >  CP009273/3639094‑3639382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑