Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,657,375 G→A 100% P202S (CCT→TCT)  gadW ← transcriptional activator of gadA and gadBC; repressor of gadX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,657,3750GA98.8% 794.3 / ‑0.1 240P202S (CCT→TCT) gadWtranscriptional activator of gadA and gadBC; repressor of gadX
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (134/103);  total (136/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

AATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAA  >  CP009273/3657229‑3657523
                                                                                                                                                  |                                                                                                                                                    
aaTATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGaaa                                                                                                                                                    >  2:1395647/1‑149 (MQ=255)
  tatTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAtt                                                                                                                                                  <  1:1090964/149‑1 (MQ=255)
  tatTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAtt                                                                                                                                                  >  2:1680219/1‑149 (MQ=255)
   atTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAttt                                                                                                                                                 >  2:2343497/1‑149 (MQ=255)
   atTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAttt                                                                                                                                                 >  2:1699590/1‑149 (MQ=255)
    ttCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTg                                                                                                                                                >  1:724929/1‑149 (MQ=255)
     tCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGa                                                                                                                                               <  2:57187/149‑1 (MQ=255)
      cAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGAc                                                                                                                                              <  2:107733/149‑1 (MQ=255)
        aaTGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAATTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGt                                                                                                                                            <  1:619600/149‑1 (MQ=255)
          tGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTaa                                                                                                                                          >  2:1027859/1‑149 (MQ=255)
           gCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAAc                                                                                                                                         >  1:3163920/1‑149 (MQ=255)
             gAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTc                                                                                                                                       >  1:4094510/1‑149 (MQ=255)
                 tatGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGt                                                                                                                                   >  1:822951/1‑149 (MQ=255)
                 tatGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGt                                                                                                                                   >  1:822765/1‑149 (MQ=255)
                   tGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTaa                                                                                                                                 <  1:1088980/149‑1 (MQ=255)
                   tGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTaa                                                                                                                                 <  1:268001/149‑1 (MQ=255)
                       aGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATTCAGAGAAATTTGACGTAACTCGAGTAATCGt                                                                                                                             >  2:2966087/1‑149 (MQ=255)
                        ggAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTc                                                                                                                            <  2:2896712/149‑1 (MQ=255)
                        ggAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTc                                                                                                                            >  1:4046528/1‑149 (MQ=255)
                         gAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCt                                                                                                                           <  2:2084878/149‑1 (MQ=255)
                           aaaGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTgg                                                                                                                         >  1:2337779/1‑149 (MQ=255)
                            aaGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGc                                                                                                                        >  2:714513/1‑149 (MQ=255)
                            aaGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGc                                                                                                                        >  1:1042997/1‑149 (MQ=255)
                             aGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGcc                                                                                                                       >  2:3070098/1‑149 (MQ=255)
                                tACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATc                                                                                                                    <  2:3752291/149‑1 (MQ=255)
                                   cTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGAc                                                                                                                 >  2:875255/1‑149 (MQ=255)
                                     ggCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACAt                                                                                                               >  2:2530108/1‑149 (MQ=255)
                                      gCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATc                                                                                                              <  2:2184752/149‑1 (MQ=255)
                                         aaTGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTg                                                                                                           >  1:2109204/1‑149 (MQ=255)
                                         aaTGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTg                                                                                                           >  1:3537114/1‑149 (MQ=255)
                                          aTGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTgg                                                                                                          >  2:3470597/1‑149 (MQ=255)
                                          aTGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTgg                                                                                                          >  2:1519775/1‑149 (MQ=255)
                                            gTTGCTCAATCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTATTCGTCTGGCCATCGACATCCTGGAg                                                                                                        <  1:3664251/149‑1 (MQ=255)
                                               gctcAAACTGATGTGGAGTTACAACATAATATTGTCGAATTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCg                                                                                                     <  2:3952867/146‑1 (MQ=255)
                                                   aaaCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTa                                                                                                 <  2:1916333/149‑1 (MQ=255)
                                                    aaCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTaa                                                                                                <  2:1284213/149‑1 (MQ=255)
                                                    aaCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTaa                                                                                                >  1:833736/1‑149 (MQ=255)
                                                     aCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAat                                                                                               >  2:1841648/1‑149 (MQ=255)
                                                     aCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAat                                                                                               >  2:3312344/1‑149 (MQ=255)
                                                     aCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAat                                                                                               <  1:24334/149‑1 (MQ=255)
                                                     aCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAat                                                                                               <  1:24516/149‑1 (MQ=255)
                                                       tGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAatat                                                                                             >  1:1900578/1‑149 (MQ=255)
                                                        gATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATAtt                                                                                            <  1:1047135/149‑1 (MQ=255)
                                                        gATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATAtt                                                                                            >  1:3450239/1‑149 (MQ=255)
                                                             ggCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACt                                                                                       >  1:2090306/1‑149 (MQ=255)
                                                              gCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTg                                                                                      <  1:2151217/149‑1 (MQ=255)
                                                               cGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGa                                                                                     <  2:3548130/149‑1 (MQ=255)
                                                                gTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGaa                                                                                    <  2:2677922/149‑1 (MQ=255)
                                                                 ttACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGaaa                                                                                   >  1:462762/1‑149 (MQ=255)
                                                                 ttACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGaaa                                                                                   >  1:1645375/1‑149 (MQ=255)
                                                                 ttACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGaaa                                                                                   >  2:296014/1‑149 (MQ=255)
                                                                  tACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAAc                                                                                  <  1:188832/149‑1 (MQ=255)
                                                                     acCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAgg                                                                               <  1:2932709/149‑1 (MQ=255)
                                                                     acCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAgg                                                                               >  2:3202382/1‑149 (MQ=255)
                                                                              ttGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCAt                                                                      >  1:3963847/1‑149 (MQ=255)
                                                                                gTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATcc                                                                    <  2:1895817/149‑1 (MQ=255)
                                                                                   gAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGc                                                                 >  2:2732143/1‑149 (MQ=255)
                                                                                    aaaTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCa                                                                <  1:201654/149‑1 (MQ=255)
                                                                                    aaaTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCa                                                                <  1:3759205/149‑1 (MQ=255)
                                                                                      aTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAAc                                                              <  2:3837587/149‑1 (MQ=255)
                                                                                      aTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAAc                                                              >  1:611029/1‑149 (MQ=255)
                                                                                      aTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAAc                                                              >  2:2455209/1‑149 (MQ=255)
                                                                                        gtgtTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACtt                                                            <  2:328207/149‑1 (MQ=255)
                                                                                        gtgtTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACtt                                                            <  2:3898587/149‑1 (MQ=255)
                                                                                           tttATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACttttt                                                         <  1:714081/149‑1 (MQ=255)
                                                                                           tttATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACttttt                                                         <  2:1447896/149‑1 (MQ=255)
                                                                                              ataAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACtttttttt                                                      <  1:2151959/149‑1 (MQ=255)
                                                                                               taAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTg                                                     <  2:850046/149‑1 (MQ=255)
                                                                                                  aGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGAtt                                                  <  2:1012939/149‑1 (MQ=255)
                                                                                                   gTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTa                                                 >  2:1443341/1‑149 (MQ=255)
                                                                                                    tACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAg                                                >  2:474821/1‑149 (MQ=255)
                                                                                                     aCGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGa                                               <  1:2409530/149‑1 (MQ=255)
                                                                                                     aCGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGa                                               >  2:2409530/1‑149 (MQ=255)
                                                                                                     aCGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGa                                               <  1:2002990/149‑1 (MQ=255)
                                                                                                      cGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGAc                                              >  1:506298/1‑149 (MQ=255)
                                                                                                        aTGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGActc                                            >  1:1074176/1‑149 (MQ=255)
                                                                                                        aTGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGActc                                            >  2:3318572/1‑149 (MQ=255)
                                                                                                        aTGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGActc                                            >  1:1599374/1‑149 (MQ=255)
                                                                                                         tGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGActct                                           >  2:2258358/1‑149 (MQ=255)
                                                                                                          gTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACtctc                                          <  1:3919215/149‑1 (MQ=255)
                                                                                                          gTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACtctc                                          >  1:1686625/1‑149 (MQ=255)
                                                                                                            aCTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGc                                        <  1:102028/149‑1 (MQ=255)
                                                                                                               gCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTgg                                     >  2:402427/1‑149 (MQ=255)
                                                                                                               gCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTgg                                     >  2:3144485/1‑149 (MQ=255)
                                                                                                                cTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGt                                    <  1:1376645/149‑1 (MQ=255)
                                                                                                                  ataGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGtat                                  >  2:1994600/1‑149 (MQ=255)
                                                                                                                    aGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATAc                                >  1:3383229/1‑149 (MQ=255)
                                                                                                                    aGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATAc                                >  2:113637/1‑149 (MQ=255)
                                                                                                                     gCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACa                               <  2:822765/149‑1 (MQ=255)
                                                                                                                        acaTTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTc                            >  1:3428119/1‑149 (MQ=255)
                                                                                                                                 cGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGa                   >  1:3606375/1‑149 (MQ=255)
                                                                                                                                   cAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGata                 >  1:1060705/1‑149 (MQ=255)
                                                                                                                                     ttAGTATGAATATAAATTTGACGTAACTCTAGTAATCTTCGGGCCATCTACATCCTGGAGGCGAGTAATTTTTTAATTAAAATGGTATTTTCTTCCTCAAAATTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATc               <  1:1445898/148‑1 (MQ=255)
                                                                                                                                      tAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATcg              >  1:1219706/1‑149 (MQ=255)
                                                                                                                                       aGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCACTGGTATACATTCTTTCCGCGATATcgc             >  1:420353/1‑149 (MQ=255)
                                                                                                                                        gTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATcgcg            <  2:621228/149‑1 (MQ=255)
                                                                                                                                          aTGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCa          >  1:3710320/1‑149 (MQ=255)
                                                                                                                                          aTGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCa          >  1:1110262/1‑149 (MQ=255)
                                                                                                                                            gCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGa        >  2:1604677/1‑149 (MQ=255)
                                                                                                                                                  gAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCaa  >  1:1300113/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAA  >  CP009273/3657229‑3657523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑