Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,661,610 A→T 100% F313L (TTT→TTA)  yhjA ← putative cytochrome C peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,661,6100AT99.6% 775.5 / ‑4.6 231F313L (TTT→TTA) yhjAputative cytochrome C peroxidase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (118/112);  total (118/113)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

CCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCAATCGGGTTCAACG  >  CP009273/3661464‑3661755
                                                                                                                                                  |                                                                                                                                                 
 cgccgAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAAt                                                                                                                                                >  1:3582113/1‑149 (MQ=255)
 cgccgAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAAt                                                                                                                                                <  1:2476060/149‑1 (MQ=255)
 cgccgAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAAt                                                                                                                                                <  1:4095287/149‑1 (MQ=255)
 cgccgAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAAt                                                                                                                                                >  1:1469280/1‑149 (MQ=255)
 cgccgAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAAt                                                                                                                                                <  2:1198622/149‑1 (MQ=255)
  gccgAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAACTGGGGAATCCGGCGTAATTAATGTTTTCTCTAAtt                                                                                                                                               >  2:1903951/1‑149 (MQ=255)
    cgAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCa                                                                                                                                             <  2:3028783/149‑1 (MQ=255)
      agaATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGc                                                                                                                                           >  2:1880873/1‑149 (MQ=255)
      agaATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTATCTAATTCAGc                                                                                                                                           >  1:539106/1‑149 (MQ=255)
        aataatACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCaa                                                                                                                                         >  2:1673025/1‑149 (MQ=255)
         ataataCCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAAt                                                                                                                                        <  2:2585568/149‑1 (MQ=255)
          taataCCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATg                                                                                                                                       <  2:218778/149‑1 (MQ=255)
          taataCCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATg                                                                                                                                       <  2:1807547/149‑1 (MQ=255)
            ataCCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGc                                                                                                                                     <  1:3749669/149‑1 (MQ=255)
                caccaTGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCa                                                                                                                                 <  1:518094/149‑1 (MQ=255)
                  ccaTGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGt                                                                                                                               <  2:1316768/149‑1 (MQ=255)
                     tGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAat                                                                                                                            <  2:1523821/149‑1 (MQ=255)
                      gACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAata                                                                                                                           <  2:2824595/149‑1 (MQ=255)
                        cAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATAtt                                                                                                                         >  2:1399678/1‑149 (MQ=255)
                        cAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATAtt                                                                                                                         >  1:3159299/1‑149 (MQ=255)
                        cAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGAGTAATAAATGTTTTCTATAATTCAGCAATGGCATCAGTAAAAtt                                                                                                                         >  1:3046785/1‑149 (MQ=255)
                         aaGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATAttt                                                                                                                        <  1:2889762/149‑1 (MQ=255)
                          aGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATAtttt                                                                                                                       >  2:2446557/1‑149 (MQ=255)
                          aGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATAtttt                                                                                                                       >  2:3779308/1‑149 (MQ=255)
                            ttGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCg                                                                                                                     >  2:802053/1‑149 (MQ=255)
                             tGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGc                                                                                                                    <  1:3876675/149‑1 (MQ=255)
                               cacaTTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCa                                                                                                                  <  1:3623492/149‑1 (MQ=255)
                                 caTTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACt                                                                                                                <  1:2136583/149‑1 (MQ=255)
                                  aTTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTg                                                                                                               <  2:3116187/149‑1 (MQ=255)
                                   tttATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGa                                                                                                              >  2:2291264/1‑149 (MQ=255)
                                      attatCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAAc                                                                                                           >  2:3270631/1‑149 (MQ=255)
                                       ttatCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGAAATGGCATCAGTAATATTTTCGCCACTGAAAcc                                                                                                          >  2:1549690/1‑149 (MQ=255)
                                        tatCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCt                                                                                                         >  1:1270533/1‑149 (MQ=255)
                                         atCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCtt                                                                                                        <  2:3670374/149‑1 (MQ=255)
                                          tCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTg                                                                                                       >  1:1080140/1‑149 (MQ=255)
                                           cTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGc                                                                                                      <  1:2562816/149‑1 (MQ=255)
                                                aataatTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATa                                                                                                 >  1:3151543/1‑149 (MQ=255)
                                                aataatTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATa                                                                                                 >  1:737836/1‑149 (MQ=255)
                                                  taatTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGa                                                                                               <  2:2511628/149‑1 (MQ=255)
                                                  taatTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGa                                                                                               <  1:73603/149‑1 (MQ=255)
                                                   aatTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGAc                                                                                              >  1:2374514/1‑149 (MQ=255)
                                                        aTAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGa                                                                                         <  2:539106/149‑1 (MQ=255)
                                                          aGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAgg                                                                                       >  2:1983443/1‑149 (MQ=255)
                                                           gCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGa                                                                                      <  2:510525/149‑1 (MQ=255)
                                                               tttttCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTg                                                                                  <  1:3443129/149‑1 (MQ=255)
                                                               tttttCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTg                                                                                  <  2:3607962/149‑1 (MQ=255)
                                                                ttttCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGc                                                                                 >  1:1375123/1‑149 (MQ=255)
                                                                     tGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGtttt                                                                            >  2:2707618/1‑149 (MQ=255)
                                                                          cgcCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCt                                                                       <  2:388973/149‑1 (MQ=255)
                                                                              gTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCgg                                                                   >  1:2268058/1‑149 (MQ=255)
                                                                                cAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGAt                                                                 <  1:884225/149‑1 (MQ=255)
                                                                                 aGCGCATTTTCATCTCCGCGCAACCATTTCTCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATc                                                                <  1:1775363/149‑1 (MQ=255)
                                                                                  gcgcATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCt                                                               <  2:1071901/149‑1 (MQ=255)
                                                                                  gcgcATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCt                                                               >  2:2289493/1‑149 (MQ=255)
                                                                                   cgcATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCtt                                                              <  2:598392/149‑1 (MQ=255)
                                                                                   cgcATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCtt                                                              <  1:3377328/149‑1 (MQ=255)
                                                                                   cgcATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCtt                                                              <  1:697066/149‑1 (MQ=255)
                                                                                     cATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCtttt                                                            >  1:3103591/1‑149 (MQ=255)
                                                                                     cATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCtttt                                                            >  1:3186136/1‑149 (MQ=255)
                                                                                      aTTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCttttt                                                           <  1:1034630/149‑1 (MQ=255)
                                                                                        tttCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTcc                                                         <  2:2520847/149‑1 (MQ=255)
                                                                                         ttCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCa                                                        <  2:737836/149‑1 (MQ=255)
                                                                                             tctcCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCtt                                                    >  2:1668196/1‑149 (MQ=255)
                                                                                             tctcCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCtt                                                    >  2:1664316/1‑149 (MQ=255)
                                                                                              ctcCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTa                                                   <  1:3997252/149‑1 (MQ=255)
                                                                                                ccGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGc                                                 <  2:2924239/149‑1 (MQ=255)
                                                                                                  gcgcAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCaa                                               >  2:2884132/1‑149 (MQ=255)
                                                                                                      aaCCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCaataat                                           <  1:2923007/149‑1 (MQ=255)
                                                                                                       aCCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAAtt                                          <  2:1089989/149‑1 (MQ=255)
                                                                                                       aCCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAAtt                                          <  1:3515046/149‑1 (MQ=255)
                                                                                                         cATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTc                                        <  1:418482/149‑1 (MQ=255)
                                                                                                          aTTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCg                                       >  1:3849676/1‑149 (MQ=255)
                                                                                                           tttATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGt                                      >  2:3447704/1‑149 (MQ=255)
                                                                                                             tATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTcc                                    >  2:937447/1‑149 (MQ=255)
                                                                                                                cAAATGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAg                                 <  2:2677851/149‑1 (MQ=255)
                                                                                                                  aaTGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGa                               <  2:369002/149‑1 (MQ=255)
                                                                                                                   aTGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGAt                              <  2:978619/149‑1 (MQ=255)
                                                                                                                   aTGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGAt                              <  1:3264517/149‑1 (MQ=255)
                                                                                                                   aTGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGAt                              >  2:154577/1‑149 (MQ=255)
                                                                                                                    tGGGGAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGAtt                             <  1:1666586/149‑1 (MQ=255)
                                                                                                                      gggAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTc                           <  1:3270631/149‑1 (MQ=255)
                                                                                                                       ggAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCg                          >  2:654843/1‑149 (MQ=255)
                                                                                                                        gAATCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGa                         <  1:1021836/149‑1 (MQ=255)
                                                                                                                         aaTCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGAc                        >  1:3545954/1‑149 (MQ=255)
                                                                                                                         aaTCCGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGAc                        >  1:2447400/1‑149 (MQ=255)
                                                                                                                             cGGCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCa                    >  1:2870540/1‑149 (MQ=255)
                                                                                                                               gCGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCAtt                  <  1:2151247/149‑1 (MQ=255)
                                                                                                                                cGTAATTAATGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCAttt                 >  2:675273/1‑149 (MQ=255)
                                                                                                                                  taattaatGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCACAGGATTTCGACGCCATTTCa               >  2:4033326/1‑149 (MQ=255)
                                                                                                                                   aattaatGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCaa              >  2:3072771/1‑149 (MQ=255)
                                                                                                                                   aattaatGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCaa              >  2:3441736/1‑149 (MQ=255)
                                                                                                                                    attaatGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCAAt             >  1:3967593/1‑149 (MQ=255)
                                                                                                                                      taatGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCAATCg           >  1:3244697/1‑149 (MQ=255)
                                                                                                                                       aatGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCAATCgg          >  1:3104489/1‑149 (MQ=255)
                                                                                                                                         tGTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCAATCGGGt        <  1:1881495/149‑1 (MQ=255)
                                                                                                                                          gTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCAATCGGGtt       >  2:615350/1‑149 (MQ=255)
                                                                                                                                          gTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCAATCGGGtt       >  2:39550/1‑149 (MQ=255)
                                                                                                                                          gTTTTCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCAATCGGGtt       <  2:1859410/149‑1 (MQ=255)
                                                                                                                                           ttttCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCAATCGGGTTc      <  2:810349/149‑1 (MQ=255)
                                                                                                                                            tttCTCTAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCAATCGGGTTCa     >  2:1566332/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGTTGCGCCGTCAGCGCATTTTCATCTCCGCGCAACCATTTATCAAATGGGGAATCCGGCGTAATTAATGTTTTCTCAAATTCAGCAATGGCATCAGTAATATTTTCGCCACTGAAACCTTGCGGATAGACTTCGAGGAACTGCGTTTTAAGCTGCGGATCTTTTTCCAGCTTAGCAATAATTTCGTCCCAGGATTTCGACGCCATTTCAATCGGGTTCAACG  >  CP009273/3661464‑3661755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑