Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,686,345 C→A 100% R751L (CGC→CTC)  bcsA ← cellulose synthase, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,686,3450CA97.7% 697.1 / 6.0 218R751L (CGC→CTC) bcsAcellulose synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (110/103);  total (112/106)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 218 total aligned reads displayed.

GCCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTCAT  >  CP009273/3686199‑3686490
                                                                                                                                                  |                                                                                                                                                 
gCCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGt                                                                                                                                                 <  2:3309224/149‑1 (MQ=255)
gCCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGt                                                                                                                                                 <  2:667374/149‑1 (MQ=255)
 ccAGAGCGCCCATGTATCCGCACGGTCAAACGTACTCTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGTCATTAATTTCAGCCCAACTTCATTACCCATCACGCTCGCCACCTGGGTAGGGAAGACGTATTCCTGCTGACCGAGtt                                                                                                                                                <  2:2042639/149‑1 (MQ=255)
 ccAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGtt                                                                                                                                                >  2:1961204/1‑149 (MQ=255)
 ccAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGtt                                                                                                                                                >  2:4051747/1‑149 (MQ=255)
   agagCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTa                                                                                                                                              <  1:3371549/149‑1 (MQ=255)
   agagCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTa                                                                                                                                              <  1:1839114/149‑1 (MQ=255)
   agagCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTa                                                                                                                                              >  1:2098972/1‑149 (MQ=255)
    gagCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTaa                                                                                                                                             >  2:1838282/1‑149 (MQ=255)
      gcgcCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGc                                                                                                                                           <  1:3729561/149‑1 (MQ=255)
            aTGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGa                                                                                                                                     <  1:630808/149‑1 (MQ=255)
            aTGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGa                                                                                                                                     <  2:3996040/149‑1 (MQ=255)
            aTGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGa                                                                                                                                     <  1:2495417/149‑1 (MQ=255)
              gTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGAtt                                                                                                                                   <  1:3258839/149‑1 (MQ=255)
              gTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGAtt                                                                                                                                   <  1:3922380/149‑1 (MQ=255)
                 tCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCACCAGATTCAc                                                                                                                                >  2:16227/1‑149 (MQ=255)
                 tCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCAc                                                                                                                                >  2:15810/1‑149 (MQ=255)
                  ccGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACt                                                                                                                               <  1:1427547/149‑1 (MQ=255)
                    gCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACttt                                                                                                                             >  1:3589702/1‑149 (MQ=255)
                     cACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTc                                                                                                                            <  1:1498618/149‑1 (MQ=255)
                            aaaCGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCtt                                                                                                                     >  1:1966179/1‑149 (MQ=255)
                              aCGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTcc                                                                                                                   >  2:3177925/1‑149 (MQ=255)
                              aCGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTcc                                                                                                                   <  2:2006593/149‑1 (MQ=255)
                              aCGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTcc                                                                                                                   >  1:2129066/1‑149 (MQ=255)
                               cGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCa                                                                                                                  <  2:1283511/149‑1 (MQ=255)
                                   cccTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAAt                                                                                                              >  1:900152/3‑149 (MQ=255)
                                   cacTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAAt                                                                                                              <  1:3960662/149‑1 (MQ=255)
                                   cacTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAAt                                                                                                              <  1:3480631/149‑1 (MQ=255)
                                     cTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCt                                                                                                            <  2:81369/149‑1 (MQ=255)
                                         acaAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTgcgc                                                                                                        <  1:3563482/149‑1 (MQ=255)
                                           aaaaTCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCt                                                                                                      >  1:1190135/1‑149 (MQ=255)
                                            aaaTCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTg                                                                                                     >  1:740567/1‑149 (MQ=255)
                                             aaTCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGa                                                                                                    >  1:1736921/1‑149 (MQ=255)
                                              aTCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGAc                                                                                                   <  2:1215994/149‑1 (MQ=255)
                                              aTCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGAc                                                                                                   <  2:3266663/149‑1 (MQ=255)
                                                 gATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGt                                                                                                <  1:1669483/149‑1 (MQ=255)
                                                 gATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGt                                                                                                <  1:2043726/149‑1 (MQ=255)
                                                 gATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGt                                                                                                <  1:727334/149‑1 (MQ=255)
                                                 gATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGt                                                                                                <  1:2329290/149‑1 (MQ=255)
                                                 gATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATGACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGt                                                                                                <  1:727311/149‑1 (MQ=255)
                                                   tatGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttg                                                                                              <  2:2409056/149‑1 (MQ=255)
                                                      gTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGAGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatc                                                                                           <  1:1780923/149‑1 (MQ=255)
                                                            gggTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatcttgatc                                                                                     <  2:300421/149‑1 (MQ=255)
                                                            gggTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatcttgatc                                                                                     >  2:2677396/1‑149 (MQ=255)
                                                                  tgAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCaaa                                                                               >  1:3254634/1‑149 (MQ=255)
                                                                   gAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAac                                                                              >  2:3851223/1‑149 (MQ=255)
                                                                   gAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTACCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAac                                                                              <  2:1017576/149‑1 (MQ=255)
                                                                       ggCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAaccacc                                                                          <  1:1553231/149‑1 (MQ=255)
                                                                          aTTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTc                                                                       <  1:2769055/149‑1 (MQ=255)
                                                                          aTTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTc                                                                       <  2:2290118/149‑1 (MQ=255)
                                                                           ttAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCg                                                                      >  2:3662390/1‑149 (MQ=255)
                                                                           ttAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCg                                                                      >  1:2379048/1‑149 (MQ=255)
                                                                             aaTTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGga                                                                    >  1:1094182/1‑149 (MQ=255)
                                                                             aaTTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGga                                                                    <  1:3706049/149‑1 (MQ=255)
                                                                              aTTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGgag                                                                   >  1:1794044/1‑149 (MQ=255)
                                                                              aTTTCAGCCCAACTTCATGACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGgag                                                                   <  2:528077/149‑1 (MQ=255)
                                                                               tttCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGgaga                                                                  >  2:1119380/1‑149 (MQ=255)
                                                                               tttCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGgaga                                                                  >  2:565255/1‑149 (MQ=255)
                                                                                 tCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGaaa                                                                <  2:2552539/149‑1 (MQ=255)
                                                                                    gCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATcc                                                             <  1:509374/149‑1 (MQ=255)
                                                                                    gCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATcc                                                             <  2:2023949/149‑1 (MQ=255)
                                                                                    gCCCAACTTCATTACCCATCACGCACGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATcc                                                             <  1:1760567/149‑1 (MQ=255)
                                                                                     cccAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCt                                                            >  1:3092750/1‑149 (MQ=255)
                                                                                        aaCTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGaa                                                         <  1:2532260/149‑1 (MQ=255)
                                                                                        aaCTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGaa                                                         <  1:2274730/149‑1 (MQ=255)
                                                                                        aaCTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGaa                                                         >  1:666358/1‑149 (MQ=255)
                                                                                         aCTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAAc                                                        >  2:1657912/1‑149 (MQ=255)
                                                                                         aCTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAAc                                                        <  2:805294/149‑1 (MQ=255)
                                                                                         aCTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAAc                                                        <  2:324214/149‑1 (MQ=255)
                                                                                              aTTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTac                                                   >  1:1336634/1‑149 (MQ=255)
                                                                                              aTTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTac                                                   >  1:1026890/1‑149 (MQ=255)
                                                                                                    cATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACgaga                                             >  2:745005/1‑149 (MQ=255)
                                                                                                     aTCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaa                                            <  1:440127/149‑1 (MQ=255)
                                                                                                      tCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaag                                           >  2:345805/1‑149 (MQ=255)
                                                                                                       cACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaaga                                          >  2:2195194/1‑149 (MQ=255)
                                                                                                       cACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaaga                                          >  2:119615/1‑149 (MQ=255)
                                                                                                       cACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaaga                                          >  2:904442/1‑149 (MQ=255)
                                                                                                        aCGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAagag                                         >  1:2672592/1‑149 (MQ=255)
                                                                                                              gcCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGAcc                                   <  1:565255/149‑1 (MQ=255)
                                                                                                               ccACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCa                                  <  1:2284284/149‑1 (MQ=255)
                                                                                                                 aCCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATc                                >  1:287907/1‑149 (MQ=255)
                                                                                                                 aCCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATc                                >  1:2626931/1‑149 (MQ=255)
                                                                                                                   cTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCtt                              <  1:2994450/149‑1 (MQ=255)
                                                                                                                    tGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTc                             >  2:1265451/1‑149 (MQ=255)
                                                                                                                     gggTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTcg                            >  1:1861633/1‑149 (MQ=255)
                                                                                                                        tCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCgg                         >  2:2995363/1‑149 (MQ=255)
                                                                                                                            gAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCac                     >  2:1044465/1‑148 (MQ=255)
                                                                                                                              aGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCAATTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAAtt                   >  2:3095598/1‑149 (MQ=255)
                                                                                                                               gACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTg                  >  1:1360762/1‑149 (MQ=255)
                                                                                                                                 cGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGcc                <  1:3851223/149‑1 (MQ=255)
                                                                                                                                   tATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCcgc              >  2:2545486/1‑149 (MQ=255)
                                                                                                                                   tATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCcgc              >  1:2545572/1‑149 (MQ=255)
                                                                                                                                      tCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCggg           <  1:3592135/149‑1 (MQ=255)
                                                                                                                                        ctgctgACCGCGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCa         <  2:2945125/149‑1 (MQ=255)
                                                                                                                                            tgACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGt     >  2:639734/1‑149 (MQ=255)
                                                                                                                                             gACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTc    >  2:2652310/1‑149 (MQ=255)
                                                                                                                                             gACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTc    >  2:1204176/1‑149 (MQ=255)
                                                                                                                                              aCCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTCa   >  2:2659996/1‑149 (MQ=255)
                                                                                                                                               ccGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACTGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCCTCGTCAt  >  2:2758355/1‑149 (MQ=255)
                                                                                                                                               ccGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTCAt  >  1:3884004/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTCAT  >  CP009273/3686199‑3686490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑