Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,487 A→C 7.8% D415E (GAT→GAG)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4870AC 7.8% 448.3 / 5.4 190D415E (GAT→GAG) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base A (32/134);  new base C (14/6);  total (49/141)
Fisher's exact test for biased strand distribution p-value = 7.08e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCA  >  CP009273/3721340‑3721635
                                                                                                                                                   |                                                                                                                                                    
aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAAt                                                                                                                                                     <  1:3295849/149‑1 (MQ=255)
 gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATc                                                                                                                                                    <  1:3191557/149‑1 (MQ=255)
 gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGGTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTAATc                                                                                                                                                    <  2:2306148/149‑1 (MQ=255)
   ggCATATCGCTGCGCATCTTGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGa                                                                                                                                                  <  2:2732621/149‑1 (MQ=255)
   ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTATTCGa                                                                                                                                                  <  2:2989110/149‑1 (MQ=255)
         aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                            <  1:139353/149‑1 (MQ=255)
         aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                            <  1:770329/149‑1 (MQ=255)
         aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGGgctgct                                                                                                                                            >  2:1734327/1‑149 (MQ=255)
          cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGctgctg                                                                                                                                           <  2:2112452/149‑1 (MQ=255)
            cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGAc                                                                                                                                         <  1:3220675/149‑1 (MQ=255)
             tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGAcc                                                                                                                                        <  1:2543804/149‑1 (MQ=255)
             tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGAcc                                                                                                                                        <  1:475574/149‑1 (MQ=255)
               cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGGCCTCGGGCTGGGGACCGc                                                                                                                                      >  1:2724775/1‑149 (MQ=255)
               cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                                                      <  2:1840178/149‑1 (MQ=255)
                    cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTGTCGTCGCCCAGTGATTGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                                                 <  1:4034395/149‑1 (MQ=255)
                    cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGACGCGATCTGCGACAGCCTTGCTGCGCCCAGTTCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                                                 <  1:1024464/149‑1 (MQ=255)
                     tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                                                                <  1:1208872/149‑1 (MQ=255)
                      ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATc                                                                                                                               <  1:103059/149‑1 (MQ=255)
                       gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                              <  2:3884595/149‑1 (MQ=255)
                         aGATGCGACTGTTCTAACGGTAGTTGCGGCAACACTTCAATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                                                            <  2:3418624/149‑1 (MQ=255)
                         aGATGCGACTGGTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                                                            <  2:538630/149‑1 (MQ=255)
                            tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                                         <  1:818887/149‑1 (MQ=255)
                               gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                                      <  1:2945154/149‑1 (MQ=255)
                                 cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                                    <  1:2419742/149‑1 (MQ=255)
                                 cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                                    <  2:1515168/149‑1 (MQ=255)
                                 cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                                    <  1:3062458/149‑1 (MQ=255)
                                   gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                                  <  1:3788608/149‑1 (MQ=255)
                                     tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                                <  2:3193200/149‑1 (MQ=255)
                                       tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGGGCCCCCGTAGGGTACTCGCGCTGCTGTCCGCTGGTATCGGCGAGCCTGTGGCGcc                                                                                                              >  2:763052/1‑149 (MQ=255)
                                                ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                                     <  1:2024030/149‑1 (MQ=255)
                                                   cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                                  <  2:2174388/149‑1 (MQ=255)
                                                     gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCGAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgc                                                                                                <  2:255138/149‑1 (MQ=255)
                                                     gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgc                                                                                                <  1:725517/149‑1 (MQ=255)
                                                         caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                                                            <  2:2937392/149‑1 (MQ=255)
                                                         caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCAGCGCCAGCCTGGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                                                            <  1:4019950/149‑1 (MQ=255)
                                                          aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTCCGGTCGTCGGGCGGCTGGGCGCTGATAGCCGCCCGCATCTGACGCCCGTGCGGAGTACGcgcccc                                                                                           >  2:2479679/1‑149 (MQ=255)
                                                            ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCCCCCCCGTACGGTCATCGAGGTGGTGTCCGCGGATGTCCGCCAGCGTCTGGCGCCCGGCCTCACGCGGcgccccgg                                                                                         >  1:2994929/1‑148 (MQ=255)
                                                               aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                                      <  2:2363762/149‑1 (MQ=255)
                                                               aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTCCGGGCCGCGCGCTGGTGGCGGCGGTGCTCCGCCGGCCGCGGACGCCAGGCCTCACGTCGCggcgcgcccc                                                                                      >  2:2689979/1‑149 (MQ=255)
                                                               aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTCCGGGACTCGTGCTGGTGGCCGCTGATCTCCGCCCGCCGCTGACGCCAGTGCTCGCGACGCggcccgcccc                                                                                      >  1:2555779/1‑149 (MQ=255)
                                                                      gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGTCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCaa                                                                               <  2:3997811/149‑1 (MQ=255)
                                                                      gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCac                                                                               <  1:2506660/149‑2 (MQ=255)
                                                                      gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCaa                                                                               <  1:1515024/149‑1 (MQ=255)
                                                                      gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCaa                                                                               <  1:3806641/149‑1 (MQ=255)
                                                                      gATTCCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCaa                                                                               <  1:3850217/149‑1 (MQ=255)
                                                                           ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa                                                                          <  1:353021/149‑1 (MQ=255)
                                                                            tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac                                                                         <  1:1584685/149‑1 (MQ=255)
                                                                               ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACt                                                                      <  1:2267726/149‑1 (MQ=255)
                                                                                  ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                                                   <  1:1281894/149‑1 (MQ=255)
                                                                                    cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                                                 <  1:1122902/149‑1 (MQ=255)
                                                                                     gccgcGATCTGCGCCAGCCTGGCGGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTTACACTTTGCgg                                                                <  2:2148133/149‑1 (MQ=255)
                                                                                       cgcgATCTGCGCCCGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAAGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                              <  2:169818/149‑1 (MQ=255)
                                                                                       cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                              <  2:2015534/149‑1 (MQ=255)
                                                                                        gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                             <  1:2408787/149‑1 (MQ=255)
                                                                                         cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa                                                            <  1:1987581/149‑1 (MQ=255)
                                                                                          gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                                                           <  1:3151157/149‑1 (MQ=255)
                                                                                           ctcagcgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTGTGCGGTTTAAt                                                          <  2:2157305/145‑1 (MQ=255)
                                                                                           aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                          <  1:3983347/149‑1 (MQ=255)
                                                                                           aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                          <  1:1653695/149‑1 (MQ=255)
                                                                                            tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                                                         <  1:2689979/149‑1 (MQ=255)
                                                                                             cTGCGCCAGCCCTGCAGCGCCCAGAGCCCGCCCCACCTCCCCCCCCCTACGGAACTCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACACTTTGCGGTTTAATAc                                                        <  2:1518994/149‑1 (MQ=255)
                                                                                                    aGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGGCGGGGATCGGGCTGGTGTCCGCGGATATCCGCCCGCCTCTGACGCCGGTCCTCACTACGCGCCCCGCCCCCACTCAACGTCACACGTTGCGGTGTCATACCGCCGGc                                                 >  2:815997/1‑149 (MQ=255)
                                                                                                       cTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg                                              <  1:2819920/149‑1 (MQ=255)
                                                                                                        atgctgcGCCCAGTGCTCGACCAACATCCCCCCCCCTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCACTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc                                             <  2:2007468/148‑1 (MQ=255)
                                                                                                         tgctgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATAGCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACGCACCGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                                            <  2:3221969/149‑1 (MQ=255)
                                                                                                          gctgcGCCCAGTGCTGGCCCCCCATCCCCCCCCGTCCGGTGCTCGGGCGGCTGACCGCTGCTAGCCGCCCGCCTCTGACGCCAGGACTCACTCCGCGCCCCGGCCCCCAGCACCGGACCCCGTTGCGGGTTAATACCGCAGGCCGGCAc                                           >  2:2556121/1‑149 (MQ=255)
                                                                                                          gctgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTGATCGAGCTGCTGACCGCTGTTATCCGCCCGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCCACGTCACACGGTGCGGGTGAATACCGCAGGCATGCAc                                           >  1:2095691/1‑149 (MQ=255)
                                                                                                          gctgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc                                           <  2:3424333/149‑1 (MQ=255)
                                                                                                          gctgcGCCCAGGCCTGGCCCCACATCCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc                                           <  2:3424265/149‑1 (MQ=255)
                                                                                                            tgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa                                         <  1:3895122/149‑1 (MQ=255)
                                                                                                              cgcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                       <  1:1144290/149‑1 (MQ=255)
                                                                                                              cgcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                       <  2:1730901/149‑1 (MQ=255)
                                                                                                              cgcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGACCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                       <  2:2170010/149‑1 (MQ=255)
                                                                                                               gcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCCGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACACGTTGCGGTTTAATACCGCAGGCCTGCACGAcct                                      >  2:1132804/1‑147 (MQ=255)
                                                                                                                cccAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                                     <  2:3925762/149‑1 (MQ=255)
                                                                                                                  cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATGCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCAACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                                   >  2:1336342/1‑149 (MQ=255)
                                                                                                                  cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTAGGCGCCCCGCCCCCAATCAACGGAACCCTTTGCGGTTTAATACCGCAGGCCGGCACGACATCCa                                   >  2:62269/1‑149 (MQ=255)
                                                                                                                  cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                                   <  1:664930/149‑1 (MQ=255)
                                                                                                                  cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                                   <  1:3163258/149‑1 (MQ=255)
                                                                                                                  cAGTGCGGGCCCCACCGCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCCTCAACGTAACACTTTGCGGTTTAAGACCTCAGGCATGCACGACATCCa                                   <  2:101750/149‑1 (MQ=255)
                                                                                                                        tGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCAt                             >  1:423035/1‑149 (MQ=255)
                                                                                                                        tGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAATACCGCCGGCATGCACGACATCCATGCCAt                             >  1:2962609/1‑149 (MQ=255)
                                                                                                                         ggCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                            <  1:1672251/149‑1 (MQ=255)
                                                                                                                           ccccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTg                          <  1:90920/149‑1 (MQ=255)
                                                                                                                               acaTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAg                      <  2:1371482/149‑1 (MQ=255)
                                                                                                                                  tCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACCTCCATGCCATCTGCCAgcgc                   >  2:250816/1‑149 (MQ=255)
                                                                                                                                   ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGGCATCTGCCAGCGCa                  <  2:1443353/149‑1 (MQ=255)
                                                                                                                                   ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa                  <  2:3685618/149‑1 (MQ=255)
                                                                                                                                   ccccccccGTACGGTAAGCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCACTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTGTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa                  <  2:1443274/149‑1 (MQ=255)
                                                                                                                                       ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAc              <  1:603774/149‑1 (MQ=255)
                                                                                                                                       ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAc              <  1:602197/149‑1 (MQ=255)
                                                                                                                                          cGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCa           >  1:2322029/1‑149 (MQ=255)
                                                                                                                                           gTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAc          >  2:701041/1‑149 (MQ=255)
                                                                                                                                               ggTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCt      >  2:942334/1‑149 (MQ=255)
                                                                                                                                                gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCtt     <  1:2078166/149‑1 (MQ=255)
                                                                                                                                                 tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc    >  1:3688790/1‑149 (MQ=255)
                                                                                                                                                 tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc    >  2:2901631/1‑149 (MQ=255)
                                                                                                                                                 tAATCGAGCTGCTGACCGATGATATCCGCCAGCATCTGACGCGAGTACTCACTACGCGCCCCGCCCCCAATCACCGTATCACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc    <  2:574040/149‑1 (MQ=255)
                                                                                                                                                  caTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTGACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCTTGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTcc   <  2:2552433/148‑1 (MQ=255)
                                                                                                                                                   aTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCa  >  1:1485460/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCA  >  CP009273/3721340‑3721635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑