Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,727,580 C→A 100% A167A (GCC→GCA)  xylH → D‑xylose ABC transporter permease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,727,5800CA98.3% 773.0 / 2.8 240A167A (GCC→GCA) xylHD‑xylose ABC transporter permease subunit
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (117/118);  total (120/120)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 245 total aligned reads displayed.

CTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTAC  >  CP009273/3727434‑3727723
                                                                                                                                                  |                                                                                                                                               
cTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGcgcagc                                                                                                                                               >  1:3597607/1‑149 (MQ=255)
cTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGcgcagc                                                                                                                                               <  1:2073221/149‑1 (MQ=255)
   ggACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGAt                                                                                                                                            <  1:3778071/149‑1 (MQ=255)
   ggACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGAt                                                                                                                                            <  1:1693657/149‑1 (MQ=255)
    gACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATg                                                                                                                                           >  2:3396306/1‑149 (MQ=255)
       tGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTca                                                                                                                                        <  1:826681/149‑1 (MQ=255)
        gCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTcac                                                                                                                                       >  2:2420102/1‑149 (MQ=255)
        gCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTcac                                                                                                                                       <  2:3228334/149‑1 (MQ=255)
        gCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTcac                                                                                                                                       <  2:1176741/149‑1 (MQ=255)
           tctcGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACaaa                                                                                                                                    >  1:1732677/1‑149 (MQ=255)
           tctcGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACaaa                                                                                                                                    >  1:883796/1‑149 (MQ=255)
           tctcGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACaaa                                                                                                                                    <  1:3005006/149‑1 (MQ=255)
            ctcGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAAt                                                                                                                                   <  1:190848/149‑1 (MQ=255)
               ggTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTgg                                                                                                                                <  1:201080/149‑1 (MQ=255)
                gTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTggg                                                                                                                               >  2:2849112/1‑149 (MQ=255)
                gTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTggg                                                                                                                               <  2:1148723/149‑1 (MQ=255)
                  gCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCa                                                                                                                             >  2:1492764/1‑149 (MQ=255)
                  gCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCa                                                                                                                             >  2:1420708/1‑149 (MQ=255)
                   ccTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCaa                                                                                                                            >  1:272641/1‑149 (MQ=255)
                    cTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCaaa                                                                                                                           <  2:2672425/149‑1 (MQ=255)
                      ggAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGc                                                                                                                         >  1:3713502/1‑149 (MQ=255)
                      ggAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGc                                                                                                                         <  2:688445/149‑1 (MQ=255)
                      ggAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGc                                                                                                                         >  2:2021415/1‑149 (MQ=255)
                      ggAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGc                                                                                                                         >  2:3559964/1‑149 (MQ=255)
                       gAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCt                                                                                                                        >  1:2875094/1‑149 (MQ=255)
                         aCGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTAt                                                                                                                      >  1:3573335/1‑149 (MQ=255)
                          cGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTAtc                                                                                                                     >  1:776654/1‑149 (MQ=255)
                          cGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTAtc                                                                                                                     <  2:4019223/149‑1 (MQ=255)
                            gATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTAtctc                                                                                                                   >  1:264073/1‑149 (MQ=255)
                             aTGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTcc                                                                                                                  <  1:3627131/149‑1 (MQ=255)
                             aTGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTcc                                                                                                                  >  1:249257/1‑149 (MQ=255)
                             aTGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTcc                                                                                                                  >  2:2947463/1‑149 (MQ=255)
                              tggtggGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTccc                                                                                                                 <  1:4037903/149‑1 (MQ=255)
                                      cgcgTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTa                                                                                                         <  2:1369438/149‑1 (MQ=255)
                                          tACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCgg                                                                                                     >  1:1039209/1‑149 (MQ=255)
                                            ccGTATAGTCGCTTGATTTATTGTCACCCTCGCGTGCATGTTGGCTTTTCGCGGCATACTGATTGGCATCACCAACGGCACGTCTGTATCCCCCTCCAGCGCTGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCt                                                                                                   <  2:385117/149‑1 (MQ=255)
                                            ccGTAAAGTCCCTTCATGTAGGGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCt                                                                                                   <  1:2552697/149‑1 (MQ=255)
                                               tAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTCCCGGCTtca                                                                                                >  2:3517093/1‑149 (MQ=255)
                                               tAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtca                                                                                                <  1:3396306/149‑1 (MQ=255)
                                                aaaGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcat                                                                                               <  1:42352/149‑1 (MQ=255)
                                                aaaGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcat                                                                                               <  1:1442040/149‑1 (MQ=255)
                                                aaaGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACAAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCACCGCCAGTACCGGCTtcat                                                                                               >  2:4051572/1‑149 (MQ=255)
                                                 aaGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcatc                                                                                              >  1:3619344/1‑149 (MQ=255)
                                                 aaGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcatc                                                                                              >  2:3843310/1‑149 (MQ=255)
                                                  aGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcatca                                                                                             >  1:50982/1‑149 (MQ=255)
                                                  aGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcatca                                                                                             >  1:3208056/1‑149 (MQ=255)
                                                  aGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcatca                                                                                             <  2:2177225/149‑1 (MQ=255)
                                                  aGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcatca                                                                                             >  2:2468608/1‑149 (MQ=255)
                                                   gTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTcatcat                                                                                            <  2:2396260/149‑1 (MQ=255)
                                                   gTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTcatcat                                                                                            <  2:3427738/149‑1 (MQ=255)
                                                      ccTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAAATGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTgg                                                                                         >  1:3069418/1‑149 (MQ=255)
                                                               aTTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTgg                                                                                >  2:59033/1‑149 (MQ=255)
                                                                ttGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGc                                                                               >  1:3803628/1‑149 (MQ=255)
                                                                ttGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGc                                                                               <  2:1732677/149‑1 (MQ=255)
                                                                ttGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGc                                                                               >  1:2926896/1‑149 (MQ=255)
                                                                ttGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGc                                                                               <  2:883731/149‑1 (MQ=255)
                                                                    cACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTaa                                                                           <  2:3034052/149‑1 (MQ=255)
                                                                    cACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTaa                                                                           >  1:448146/1‑149 (MQ=255)
                                                                    cACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTaa                                                                           >  2:2688947/1‑149 (MQ=255)
                                                                     aCCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAAt                                                                          <  2:805423/149‑1 (MQ=255)
                                                                      cccTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATg                                                                         >  1:3157163/1‑149 (MQ=255)
                                                                       ccTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATgg                                                                        >  1:3565954/1‑149 (MQ=255)
                                                                        cTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGc                                                                       >  2:2721122/1‑149 (MQ=255)
                                                                              ggCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTttgt                                                                 >  1:2199019/1‑149 (MQ=255)
                                                                               gCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTttgtt                                                                <  2:3317435/149‑1 (MQ=255)
                                                                               gCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTttgtt                                                                >  1:799259/1‑149 (MQ=255)
                                                                               gCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGACAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTttgtt                                                                >  2:53876/1‑149 (MQ=255)
                                                                                cATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTttgttg                                                               <  2:767545/149‑1 (MQ=255)
                                                                                      ggCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTCATGGCTTTTGTTGGTTGGc                                                         >  1:931813/1‑149 (MQ=255)
                                                                                      ggCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGc                                                         <  2:3637258/149‑1 (MQ=255)
                                                                                      ggCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGc                                                         <  2:3975771/149‑1 (MQ=255)
                                                                                                ggCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCgg                                               <  1:882252/149‑1 (MQ=255)
                                                                                                 gCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGga                                              >  1:1751707/1‑149 (MQ=255)
                                                                                                    tACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGgaaga                                           <  1:109284/149‑1 (MQ=255)
                                                                                                       tCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTTGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATg                                        <  1:2420102/149‑1 (MQ=255)
                                                                                                        cATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgc                                       >  1:1360546/1‑149 (MQ=255)
                                                                                                         aTTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgcg                                      >  2:3651343/1‑149 (MQ=255)
                                                                                                          ttGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgcgc                                     <  2:3183100/149‑1 (MQ=255)
                                                                                                          ttGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgcgc                                     <  1:2576370/149‑1 (MQ=255)
                                                                                                          ttGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgcgc                                     <  2:647095/149‑1 (MQ=255)
                                                                                                           tGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGcc                                    >  2:1541946/1‑149 (MQ=255)
                                                                                                                tCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAg                               >  1:3190074/1‑149 (MQ=255)
                                                                                                                  aCCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGc                             >  1:997718/1‑149 (MQ=255)
                                                                                                                  aCCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGc                             <  1:648236/149‑1 (MQ=255)
                                                                                                                  aCCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGc                             <  1:649202/149‑1 (MQ=255)
                                                                                                                   ccAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCt                            >  2:2989057/1‑149 (MQ=255)
                                                                                                                        ggCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTggg                       <  2:3295170/149‑1 (MQ=255)
                                                                                                                         gCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGt                      >  1:1832143/1‑149 (MQ=255)
                                                                                                                          cACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGtt                     <  2:1322131/149‑1 (MQ=255)
                                                                                                                           aCGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGttt                    <  1:3488923/149‑1 (MQ=255)
                                                                                                                            cGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTa                   <  1:2453320/149‑1 (MQ=255)
                                                                                                                             gACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTAc                  <  2:906995/149‑1 (MQ=255)
                                                                                                                             gACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTGGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTAc                  >  1:748126/1‑149 (MQ=255)
                                                                                                                                   aTCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGACGAATGCGCCGTCAGGCTTTGGGTTTACAGtctc            >  2:2534558/1‑149 (MQ=255)
                                                                                                                                        ccaccaGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGcc       >  1:1528461/1‑149 (MQ=255)
                                                                                                                                        ccaccaGCGCAGCGATGTCACAAAATGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTGTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGcc       >  2:1627429/1‑149 (MQ=255)
                                                                                                                                          accaGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCctc     >  1:3323658/1‑149 (MQ=255)
                                                                                                                                           ccaGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCctct    <  2:1039209/149‑1 (MQ=255)
                                                                                                                                            cagcgcagcgATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTa   <  1:3924837/149‑1 (MQ=255)
                                                                                                                                             aGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATTGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTTGGTTTACAGTCTCCGGCCTCTAc  <  2:3355421/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTAC  >  CP009273/3727434‑3727723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑