BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,814,866	A→C	6.0%	T27P (ACC→CCC)	gmk →	guanylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,814,866	0	A	C	6.0%	860.6 / 42.2	299	T27P (ACC→CCC)	gmk	guanylate kinase
Reads supporting (aligned to +/- strand): ref base A (126/155); new base C (14/4); total (140/159)
Fisher's exact test for biased strand distribution p-value = 7.36e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 313 total aligned reads displayed.

ACATGGCCGCGCCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGAACACGCAGA  >  CP009273/3814718‑3815014

   tGGCCGCGCCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAAccc                                                                                                                                                   <  2:2509017/149‑1 (MQ=255)

   tGGCCGCGCCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAAccc                                                                                                                                                   >  1:1482008/1‑149 (MQ=255)

     gCCGCGCCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCaa                                                                                                                                                 <  2:3357643/149‑1 (MQ=255)

      ccGCGCCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAAc                                                                                                                                                <  1:1246532/149‑1 (MQ=255)

       cgcgCCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAAcc                                                                                                                                               >  2:3595869/1‑149 (MQ=255)

          gcCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGtt                                                                                                                                            <  1:2774960/149‑1 (MQ=255)

          gcCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGtt                                                                                                                                            <  2:1660690/149‑1 (MQ=255)

           ccGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATTCTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTg                                                                                                                                           <  2:1161152/149‑1 (MQ=255)

           ccGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTg                                                                                                                                           <  2:2607448/149‑1 (MQ=255)

           ccGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTg                                                                                                                                           <  2:1522484/149‑1 (MQ=255)

           ccGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTg                                                                                                                                           <  2:3213130/149‑1 (MQ=255)

            cGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGt                                                                                                                                          >  1:3691441/1‑149 (MQ=255)

            cGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTAATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGt                                                                                                                                          >  1:943028/1‑149 (MQ=255)

              tgtATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTAt                                                                                                                                        >  1:3001431/1‑149 (MQ=255)

              tgtATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTAt                                                                                                                                        >  1:664475/1‑149 (MQ=255)

              tgtATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGATTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACAGTTGTAt                                                                                                                                        >  2:2811151/1‑149 (MQ=255)

                   aataaGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGacac                                                                                                                                   >  2:4020522/1‑149 (MQ=255)

                     taaGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACAccc                                                                                                                                 >  2:3839113/1‑149 (MQ=255)

                     taaGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACAccc                                                                                                                                 <  1:2769022/149‑1 (MQ=255)

                         cTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGt                                                                                                                             <  2:705342/149‑1 (MQ=255)

                            gTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTc                                                                                                                          >  1:244249/1‑149 (MQ=255)

                                gTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGtt                                                                                                                      <  2:2092311/149‑1 (MQ=255)

                                gTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGtt                                                                                                                      >  1:1887887/1‑149 (MQ=255)

                                 tAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGttt                                                                                                                     >  2:507422/1‑149 (MQ=255)

                                  aGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTc                                                                                                                    >  2:1593989/1‑149 (MQ=255)

                                   ggCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTca                                                                                                                   <  1:2677329/149‑1 (MQ=255)

                                       ttATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTcacaca                                                                                                               <  1:2374390/149‑1 (MQ=255)

                                          tttCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCAcacacca                                                                                                            >  1:3306279/1‑149 (MQ=255)

                                            tCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCAcacaccaca                                                                                                          <  2:1748972/149‑1 (MQ=255)

                                                tAATCACATACTAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGcc                                                                                                      <  2:628867/149‑1 (MQ=255)

                                                tAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGcc                                                                                                      >  2:1083261/1‑149 (MQ=255)

                                                 aaTCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCa                                                                                                     <  1:479096/149‑1 (MQ=255)

                                                   tCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAAc                                                                                                   >  1:1048051/1‑149 (MQ=255)

                                                    cacaTACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCCGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAAcc                                                                                                  <  2:940233/149‑1 (MQ=255)

                                                      caTACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAACCCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACcgc                                                                                                >  2:1924184/1‑149 (MQ=255)

                                                          cGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTcc                                                                                            <  2:645114/149‑1 (MQ=255)

                                                           gAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTAGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGACCt                                                                                           >  1:111007/1‑149 (MQ=255)

                                                            aaaGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTg                                                                                          >  1:2199488/1‑149 (MQ=255)

                                                              aGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGt                                                                                        >  1:733655/1‑149 (MQ=255)

                                                               gATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTg                                                                                       <  2:1486356/149‑1 (MQ=255)

                                                               gATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTg                                                                                       >  1:1528292/1‑149 (MQ=255)

                                                               gATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTg                                                                                       >  2:3204740/1‑149 (MQ=255)

                                                                aTACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGa                                                                                      <  2:3288010/149‑1 (MQ=255)

                                                                  aCTAATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAg                                                                                    >  1:1624739/1‑149 (MQ=255)

                                                                     cATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTcc                                                                                 >  2:614662/1‑149 (MQ=255)

                                                                     cATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTcc                                                                                 >  2:1217464/1‑149 (MQ=255)

                                                                      aTGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCa                                                                                <  2:170866/149‑1 (MQ=255)

                                                                         gCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACgg                                                                             >  2:2725872/1‑149 (MQ=255)

                                                                         gCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACgg                                                                             >  2:911011/1‑149 (MQ=255)

                                                                          cTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGt                                                                            <  1:3115001/149‑1 (MQ=255)

                                                                           tCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTg                                                                           <  1:3195425/149‑1 (MQ=255)

                                                                              aGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAAc                                                                        >  2:1319572/1‑149 (MQ=255)

                                                                              aGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAAc                                                                        <  1:3555337/149‑1 (MQ=255)

                                                                                gCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACat                                                                      <  1:3123608/149‑1 (MQ=255)

                                                                                  aCGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACatta                                                                    >  1:1389850/1‑149 (MQ=255)

                                                                                   cGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACattat                                                                   >  2:2446940/1‑149 (MQ=255)

                                                                                    gCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACattatt                                                                  <  2:2555249/149‑1 (MQ=255)

                                                                                      tttATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTc                                                                >  1:3730818/1‑149 (MQ=255)

                                                                                       ttATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCt                                                               <  1:2284714/149‑1 (MQ=255)

                                                                                        tataTTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCtt                                                              <  2:1603657/149‑1 (MQ=255)

                                                                                        tataTTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCtt                                                              >  2:1011711/1‑149 (MQ=255)

                                                                                         atatTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCttt                                                             <  2:870984/149‑1 (MQ=255)

                                                                                              gTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTaa                                                        <  2:3460107/149‑1 (MQ=255)

                                                                                              gTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTaa                                                        >  1:3346799/1‑149 (MQ=255)

                                                                                                ttCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATc                                                      <  2:3969228/149‑1 (MQ=255)

                                                                                                 tCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCa                                                     <  1:2965675/149‑1 (MQ=255)

                                                                                                 tCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCa                                                     <  1:1167626/149‑1 (MQ=255)

                                                                                                   tGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCatg                                                   <  2:3001431/149‑1 (MQ=255)

                                                                                                     cccccAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCatgat                                                 <  1:4054855/149‑1 (MQ=255)

                                                                                                        ccAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGaa                                              >  2:1197148/1‑149 (MQ=255)

                                                                                                              gcgcGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTaaa                                        <  2:2327022/149‑1 (MQ=255)

                                                                                                                gcgGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGa                                      <  2:3306289/149‑1 (MQ=255)

                                                                                                                  gggTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGaaa                                    <  1:1111472/149‑1 (MQ=255)

                                                                                                                   ggTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAAt                                   >  2:2189739/1‑149 (MQ=255)

                                                                                                                   ggTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAAt                                   <  1:3324/149‑1 (MQ=255)

                                                                                                                     tAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGa                                 <  1:1807402/149‑1 (MQ=255)

                                                                                                                     tAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGa                                 <  1:1717293/149‑1 (MQ=255)

                                                                                                                     tAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGa                                 >  1:3444120/1‑149 (MQ=255)

                                                                                                                        aTCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTa                              >  2:2204438/1‑149 (MQ=255)

                                                                                                                        aTCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTa                              <  1:1594089/149‑1 (MQ=255)

                                                                                                                         tCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGCTTTCTGTTTCACACAACACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTat                             >  1:3372902/1‑148 (MQ=255)

                                                                                                                          ccAGCCTGATTCAGGCTTTATTAAAAACCCAACCGGTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGc                            <  2:1117790/149‑1 (MQ=255)

                                                                                                                           cAGCCTGATTCAGGCTTTATTAAAACCCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCa                           >  1:2518044/1‑149 (MQ=255)

                                                                                                                             gCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCaga                         >  2:2246437/1‑149 (MQ=255)

                                                                                                                             gCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCaga                         >  1:1914480/1‑149 (MQ=255)

                                                                                                                             gCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCaga                         <  2:283460/149‑1 (MQ=255)

                                                                                                                              ccTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCagag                        <  1:1304308/149‑1 (MQ=255)

                                                                                                                                  aTTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGc                    <  1:1200606/149‑1 (MQ=255)

                                                                                                                                  aTTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGc                    <  1:767320/149‑1 (MQ=255)

                                                                                                                                    tCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGt                  <  2:1642794/149‑1 (MQ=255)

                                                                                                                                    tCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGt                  <  1:2137127/149‑1 (MQ=255)

                                                                                                                                     cAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGtt                 >  2:3855974/1‑149 (MQ=255)

                                                                                                                                         cTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTc             <  1:2567619/149‑1 (MQ=255)

                                                                                                                                           ttattaAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGa           >  1:3921716/1‑149 (MQ=255)

                                                                                                                                           ttattaAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGa           >  1:3874447/1‑149 (MQ=255)

                                                                                                                                                 aaaaCCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGAACACGc     >  1:454965/1‑149 (MQ=255)

                                                                                                                                                  aaaCCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGAACACGCa    >  2:4076289/1‑149 (MQ=255)

                                                                                                                                                    aCCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGAACACGCAGa  <  2:3784933/149‑1 (MQ=255)

                                                                                                                                                    aCCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGAACACGCAGa  <  2:1842968/149‑1 (MQ=255)

                                                                                                                                                    aCCCAACCGTTGGATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGAACACGCAGa  <  2:2918003/149‑1 (MQ=255)

ACATGGCCGCGCCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGAACACGCAGA  >  CP009273/3814718‑3815014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`