Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,818,010 C→A 100% H46N (CAT→AAT)  trmH → tRNA mG18‑2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,818,0100CA99.6% 918.0 / ‑4.5 280H46N (CAT→AAT) trmHtRNA mG18‑2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (151/128);  total (152/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 280 total aligned reads displayed.

ATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCT  >  CP009273/3817864‑3818155
                                                                                                                                                  |                                                                                                                                                 
aTTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAAt                                                                                                                                                 <  2:369573/149‑1 (MQ=255)
aTTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAAt                                                                                                                                                 >  2:3673801/1‑149 (MQ=255)
 ttAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATg                                                                                                                                                <  1:3669504/149‑1 (MQ=255)
   aaTGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGaa                                                                                                                                              <  1:2923822/149‑1 (MQ=255)
   aaTGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCNGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGaa                                                                                                                                              >  1:2083651/1‑149 (MQ=255)
    aTGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAg                                                                                                                                             <  1:2993589/149‑1 (MQ=255)
    aTGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAg                                                                                                                                             >  2:1316073/1‑149 (MQ=255)
     tGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGt                                                                                                                                            >  1:1300672/1‑149 (MQ=255)
     tGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGt                                                                                                                                            >  1:3880345/1‑149 (MQ=255)
     tGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGt                                                                                                                                            >  1:840258/1‑149 (MQ=255)
      gTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTGTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGtt                                                                                                                                           >  2:3987334/1‑149 (MQ=255)
       ttttATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTc                                                                                                                                          <  2:3933546/149‑1 (MQ=255)
            tGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGcc                                                                                                                                     >  1:2276866/1‑149 (MQ=255)
                 ccAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGTCGTTGGCGTAAATGAAGTTCACGCCGTCTg                                                                                                                                >  2:1238564/1‑149 (MQ=255)
                 ccAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTg                                                                                                                                <  1:471387/149‑1 (MQ=255)
                   aaCACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGc                                                                                                                              <  1:984753/149‑1 (MQ=255)
                     cacGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCt                                                                                                                            <  1:2306150/149‑1 (MQ=255)
                        gTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGt                                                                                                                         <  1:2426009/149‑1 (MQ=255)
                        gTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGt                                                                                                                         <  1:576906/149‑1 (MQ=255)
                        gTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGt                                                                                                                         <  1:2783175/149‑1 (MQ=255)
                         ttATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTa                                                                                                                        >  2:880384/1‑149 (MQ=255)
                         ttATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAAGGAAGTTCACGCCGTCTGGCCTGTTa                                                                                                                        >  2:221660/1‑149 (MQ=255)
                           aTGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGc                                                                                                                      >  2:1938551/1‑149 (MQ=255)
                           aTGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGc                                                                                                                      >  2:21004/1‑149 (MQ=255)
                           aTGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGc                                                                                                                      <  2:2784945/149‑1 (MQ=255)
                            tGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGcc                                                                                                                     >  1:2751798/1‑149 (MQ=255)
                            tGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGcc                                                                                                                     >  1:2672792/1‑149 (MQ=255)
                            tGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGcc                                                                                                                     >  1:1399412/1‑149 (MQ=255)
                            tGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGcc                                                                                                                     >  1:3587734/1‑149 (MQ=255)
                             gCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCg                                                                                                                    >  1:892156/1‑149 (MQ=255)
                             gCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCg                                                                                                                    >  2:1297675/1‑149 (MQ=255)
                             gCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCg                                                                                                                    >  1:892639/1‑149 (MQ=255)
                                cGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCAt                                                                                                                 <  1:344571/149‑1 (MQ=255)
                                    tCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATgcgc                                                                                                             >  1:1937145/1‑149 (MQ=255)
                                    tCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATgcgc                                                                                                             >  2:3922286/1‑149 (MQ=255)
                                         gAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCAt                                                                                                        >  2:2314689/1‑149 (MQ=255)
                                         gAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCAt                                                                                                        <  1:1495357/149‑1 (MQ=255)
                                            aTGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGc                                                                                                     <  2:667171/149‑1 (MQ=255)
                                             tGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCt                                                                                                    >  2:291922/1‑149 (MQ=255)
                                              gCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCtt                                                                                                   <  2:1774338/149‑1 (MQ=255)
                                                tCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCg                                                                                                 <  2:1293010/149‑1 (MQ=255)
                                                   ccAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCa                                                                                              <  1:4109466/149‑1 (MQ=255)
                                                     aGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAgc                                                                                            <  1:2745389/149‑1 (MQ=255)
                                                      ggcggcAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAgcg                                                                                           >  2:1499125/1‑149 (MQ=255)
                                                      ggcggcAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAgcg                                                                                           >  2:1056496/1‑149 (MQ=255)
                                                         ggcAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAgcggcg                                                                                        <  1:2611981/149‑1 (MQ=255)
                                                               cTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGgtagt                                                                                  >  1:4068038/1‑149 (MQ=255)
                                                               cTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGgtagt                                                                                  <  1:2836868/149‑1 (MQ=255)
                                                                tGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGgtcgta                                                                                 >  1:804799/1‑149 (MQ=255)
                                                                     tGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTACCAGc                                                                            >  2:612906/1‑149 (MQ=255)
                                                                      gACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCt                                                                           <  2:36031/149‑1 (MQ=255)
                                                                      gACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCt                                                                           <  1:2366293/149‑1 (MQ=255)
                                                                       aCCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTg                                                                          <  1:1908256/149‑1 (MQ=255)
                                                                         cGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTggg                                                                        <  1:1744742/149‑1 (MQ=255)
                                                                          gTCTGCATGGAGCAGGTCCACAAACCTCATATCGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGt                                                                       <  1:3072334/149‑1 (MQ=255)
                                                                             tGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACa                                                                    <  2:1378156/149‑1 (MQ=255)
                                                                              gCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAg                                                                   >  1:2138657/1‑149 (MQ=255)
                                                                                aTGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGt                                                                 <  2:858507/149‑1 (MQ=255)
                                                                                 tGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTg                                                                >  2:2456405/1‑149 (MQ=255)
                                                                                    aGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGaaa                                                             >  2:1174104/1‑149 (MQ=255)
                                                                                     gCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGaaaa                                                            >  2:2500347/1‑149 (MQ=255)
                                                                                       aGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAaca                                                          <  1:1123173/149‑1 (MQ=255)
                                                                                        ggTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAacac                                                         >  2:3392538/1‑149 (MQ=255)
                                                                                        ggTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAacac                                                         <  1:673989/149‑1 (MQ=255)
                                                                                          tCCACAAACCTCATCACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAacacac                                                       <  1:1176282/149‑1 (MQ=255)
                                                                                             acaAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGc                                                    >  2:3334997/1‑149 (MQ=255)
                                                                                               aaaCCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTCGTAACAGCTGGGTACAGGTGAAAACACACCGCAc                                                  >  1:1590391/1‑149 (MQ=255)
                                                                                                 aCCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCCTGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCa                                                >  1:3939712/1‑149 (MQ=255)
                                                                                                  ccTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCAt                                               <  1:2945644/149‑1 (MQ=255)
                                                                                                   cTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCAtt                                              >  1:3565774/1‑149 (MQ=255)
                                                                                                   cTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCAtt                                              <  1:2019529/149‑1 (MQ=255)
                                                                                                   cTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCAtt                                              <  1:2554171/149‑1 (MQ=255)
                                                                                                    tCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTg                                             <  2:464850/149‑1 (MQ=255)
                                                                                                    tCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTg                                             <  2:3190285/149‑1 (MQ=255)
                                                                                                     cATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTgg                                            <  1:3025724/149‑1 (MQ=255)
                                                                                                       tAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCg                                          >  1:637486/1‑149 (MQ=255)
                                                                                                        aaCGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGa                                         <  1:2869242/149‑1 (MQ=255)
                                                                                                         aCGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGAt                                        <  2:2083651/149‑1 (MQ=255)
                                                                                                         aCGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGAt                                        <  1:4086588/149‑1 (MQ=255)
                                                                                                          cGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATg                                       <  2:2023456/149‑1 (MQ=255)
                                                                                                           gTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGc                                      >  2:738903/1‑149 (MQ=255)
                                                                                                           gTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGc                                      >  2:2172110/1‑149 (MQ=255)
                                                                                                                 gCGATTATTCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGc                                >  1:3491776/1‑149 (MQ=255)
                                                                                                                    attattCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTCCAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCa                             >  2:419155/1‑149 (MQ=255)
                                                                                                                    attattCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCa                             >  2:3007377/1‑149 (MQ=255)
                                                                                                                     ttattCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCAt                            <  2:2781143/149‑1 (MQ=255)
                                                                                                                       attCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCAtct                          >  2:839563/1‑149 (MQ=255)
                                                                                                                        ttCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCAtctc                         >  1:4057426/1‑149 (MQ=255)
                                                                                                                        ttCGTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCAtctc                         >  1:4016102/1‑149 (MQ=255)
                                                                                                                           gTACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCaaa                      <  2:3854922/149‑1 (MQ=255)
                                                                                                                            tACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAg                     >  2:1673514/1‑149 (MQ=255)
                                                                                                                            tACCGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAg                     <  2:4001937/149‑1 (MQ=255)
                                                                                                                              ccGCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGc                   <  1:3733773/149‑1 (MQ=255)
                                                                                                                                gCAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGGCGCTCAGCTCAAAGGccc                 >  2:2708982/1‑148 (MQ=255)
                                                                                                                                 cAGATGCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAg                >  2:3475312/1‑149 (MQ=255)
                                                                                                                                      gCCGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCAt           >  2:1490265/1‑149 (MQ=255)
                                                                                                                                        cGTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGc         <  2:3619685/149‑1 (MQ=255)
                                                                                                                                         gTTGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCa        <  2:2650168/149‑1 (MQ=255)
                                                                                                                                          ttGGCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAg       >  1:1616947/1‑149 (MQ=255)
                                                                                                                                             gCGTAAATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGAtt    <  2:1914260/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCT  >  CP009273/3817864‑3818155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑