BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,822,340	A→C	20.6%	Q12H (CAA→CAC)	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,822,340	0	A	C	20.6%	265.1 / 23.5	191	Q12H (CAA→CAC)	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base A (84/63); new base C (13/31); total (97/94)
Fisher's exact test for biased strand distribution p-value = 1.80e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

ATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822193‑3822488

  aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCCGAAAATGCGCAACCg                                                                                                                                                   >  2:983995/1‑149 (MQ=255)

    aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTGTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 <  2:3438388/149‑1 (MQ=255)

       aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTgc                                                                                                                                              >  1:2252627/1‑149 (MQ=255)

         tgattttATCTCATTTAAATATTTTGATGAGGATGTGGCAAACTTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGGTTgcgc                                                                                                                                            <  1:3170599/145‑1 (MQ=255)

           cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAg                                                                                                                                          >  1:2622325/1‑149 (MQ=255)

            aTTTATCTAATTTAAATATTTAGATGTGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGATAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGa                                                                                                                                         <  1:213438/149‑1 (MQ=255)

                aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGAGCAGACTCa                                                                                                                                     >  2:3498082/1‑149 (MQ=255)

                  ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACACGGTTGCGCAGACTCaaa                                                                                                                                   >  2:3575387/1‑149 (MQ=255)

                   tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaaa                                                                                                                                  >  2:1316012/1‑149 (MQ=255)

                    cttttAATTATTTAGTTGTGGATGGGGCAAACGTTTGATTTTCTCTTTCGGTAAGCTAAACGGCCCGCTTTGCTACCACGGTATTTTTTTCTATGTCTGTTTCCACCCTCGATTCACAAAATGCGCACCCGGTTGCGCAGACTCAAAAc                                                                                                                                 <  2:1773189/147‑1 (MQ=255)

                     aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACa                                                                                                                                >  2:2667008/1‑149 (MQ=255)

                     aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                                <  2:2733966/149‑1 (MQ=255)

                      tttAAATATTTAGATGAGGATGTGGCAAACGATTTCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAATATGCGCAACCGGTTGCGCAGACTCaaatgcg                                                                                                                               >  2:4066084/1‑145 (MQ=255)

                       ttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGc                                                                                                                              >  2:1585116/1‑149 (MQ=255)

                        tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGGTGCGCAGACACAAAAAAGCg                                                                                                                             >  1:1908319/1‑149 (MQ=255)

                         aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCACAATCAAAAAATCGa                                                                                                                            >  1:2338538/1‑149 (MQ=255)

                           atatTTAGATGAGGTTGTGGCAAACGTGTGCTTTGCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTTTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                          <  2:1463264/149‑1 (MQ=255)

                           atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCAGGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                          <  2:78056/149‑1 (MQ=255)

                           atatTTAGATGAGGATGTGGAAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGTTGCGCAGACGCAAAACAGCGAAc                                                                                                                          >  2:2759537/1‑149 (MQ=255)

                            tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCACACTCAAAACaaccacct                                                                                                                         >  1:286733/1‑142 (MQ=255)

                              tttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCCAACTGa                                                                                                                       >  2:3565382/1‑149 (MQ=255)

                               ttAGTTGTGGTTGTGGAAACCGTTGGATTTTCTCTTTCGGTCAGATAAAATGCCCGGTTTGCTACCACGGGTTTGTTTTGGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGAt                                                                                                                      <  1:1367900/149‑1 (MQ=255)

                                  gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                   >  2:2441037/1‑149 (MQ=255)

                                   aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGGCCCCGGTTGCGCCGACGCAAAAAACCGCACTGATTTaa                                                                                                                  >  1:3453812/1‑148 (MQ=255)

                                   aTGAGGAGGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                  <  2:48644/149‑1 (MQ=255)

                                     gAGGATGTGGAAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCg                                                                                                                <  2:3690147/149‑1 (MQ=255)

                                       ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                              >  2:865759/1‑149 (MQ=255)

                                       ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                              >  2:1365018/1‑149 (MQ=255)

                                         aTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACTCAAAACAGCGAACAGATTTACCGTCtt                                                                                                            >  2:1538782/1‑149 (MQ=255)

                                           gtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa                                                                                                          >  2:3310378/1‑149 (MQ=255)

                                                 aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCGTTAAGATCg                                                                                                    >  2:2816660/1‑149 (MQ=255)

                                                 aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGGTGCGCAGACTCAAAAAACCGAACAGATTTAACCTCTTGAAGATCg                                                                                                    >  1:2582364/1‑149 (MQ=255)

                                                   ggTTTGCTTTTGTCTTTCGGTCAGATAAAAGGCCCGCTTTGCTACCAGGGGTTTGTTTTCGTTGTGTGTTACCACCCTCGAGTCACAAATTGCCCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTc                                                                                                  <  1:119836/148‑1 (MQ=255)

                                                   cGTTTGCTTTTCTCTTTCGTTCAGATAAAATGCCCTCTTTGCAACCACGGGATTTTTTTCGATGTGTGTTTCCACCCTCGTGACAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTc                                                                                                  <  2:907427/149‑1 (MQ=255)

                                                         cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGTTGCGCAGACTCAAAACACCGAACTCCTTTAACGTCTTGAATATAGGCcgccgc                                                                                            >  1:3984081/1‑149 (MQ=255)

                                                          ttttCTCTTTCGGACCTCTAAAATGCCCGGTTTGCTACCAGGGGTTTGTTTTCGTTGTTTTTGACCCCCCTCGAGCCCTAAATTGCGCACCGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                           <  1:534109/149‑1 (MQ=255)

                                                            ttCTGTTTCGGTCAGATAAAAGGCCCGTTTTGCTACCTGGGTTTTGTTTTCGTTGTCTTTTTCCACCCTCGTGTCATAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                         <  1:3402682/149‑1 (MQ=255)

                                                            ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                         <  2:782163/149‑1 (MQ=255)

                                                            ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                         <  2:782117/149‑1 (MQ=255)

                                                            ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTc                                                                                         >  2:186291/1‑149 (MQ=255)

                                                            ttCTCTTGCGGACACCTAAACGCCCCGCTTTGCTACCAGGGTTTTGTTTTCGTTGTGTGTTTCCACCCGCGAGTCATAATCTGCGCAACGGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                         <  1:3690840/149‑1 (MQ=255)

                                                            atctcTTTCGGTCAGATAAAATGCCCGTTTTGCTACCAGGGGTTTGTTTGCGTTGTGTTTCCCCCCCCTCTAGACAAAAATGGCGCACCGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                         <  1:781119/148‑1 (MQ=255)

                                                             tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                        >  2:3460301/1‑149 (MQ=255)

                                                             tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                        <  2:1130841/149‑1 (MQ=255)

                                                             tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTcc                                                                                        >  2:1414132/1‑149 (MQ=255)

                                                              ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                       <  2:1292707/149‑1 (MQ=255)

                                                              ctctTTCGGGCAGCTAACATGCCCGCTTGGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                       <  2:2310121/149‑1 (MQ=255)

                                                                    cGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcc                                                                                 <  2:2373484/149‑1 (MQ=255)

                                                                         agaaaaaaTGCCCGATTTGCTACCAGGGTTTTTTTTTCTTTGTCTGTTCCCACCCTCTAGTCAGAAATTGCGCACCCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            <  1:193062/145‑1 (MQ=255)

                                                                         aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCCGACTCAAAACAGCGAACTGATTTTACGTCTTGTAGATCGTCCGCCGCGTCCTCAAACCCGGtt                                                                            >  1:2575564/1‑149 (MQ=255)

                                                                         aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGCTTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            >  2:1888404/1‑149 (MQ=255)

                                                                         aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAAAAACGAACAGATTTAAAGTCTTTTGGATCGTCCGCCGCTTCCTCAAAACCTGtt                                                                            >  1:471202/1‑149 (MQ=255)

                                                                         aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            >  1:2692405/1‑149 (MQ=255)

                                                                          gCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACTCAAAACAACGAACTGATTTACCGTCTTGAATATCGTGCCCCCCGTCCTCAAACCCGGttt                                                                           >  1:1013858/1‑149 (MQ=255)

                                                                            tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                         <  2:200773/149‑1 (MQ=255)

                                                                            tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGTAGATCGTCCGCCGCGTCCTCAAACCCTGTTTgc                                                                         >  2:2631551/1‑149 (MQ=255)

                                                                            tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                         >  2:562104/1‑149 (MQ=255)

                                                                            tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                         <  2:3924891/149‑1 (MQ=255)

                                                                            tAAAATGCCCGCTTGGCTACCACGGGATTGTTTGCGATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                         <  2:2993953/149‑1 (MQ=255)

                                                                                aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGTTgccgcc                                                                     >  2:2861066/1‑149 (MQ=255)

                                                                                        tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGtcatca                                                             <  2:2257226/149‑1 (MQ=255)

                                                                                              aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgct                                                       >  2:1948755/1‑149 (MQ=255)

                                                                                               ccACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCACACTCAAAAAACCGAACAGAATTAAAAAAATTAAGATAGTCCCCCCCGTCATCAAACCAAGTTTGGCGGCAGTGAGCaaattcag                                                      >  1:811051/1‑142 (MQ=255)

                                                                                                 aCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGACAAAAAAAAACGAAATGATTTTCCGTCTTGAAGATAGACCGCCGCTTCCGCAAAAACACTTTGCCGCCTGTCAGCATATGCTGGc                                                    >  1:544621/1‑149 (MQ=255)

                                                                                                    gttttGTTTTCGTTGTCTGATACCACCCTCGAGACAGAAAATGCCCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCAGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGAt                                                 <  1:983995/146‑1 (MQ=255)

                                                                                                    ggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCCGCATCTGCTGGCGAt                                                 >  2:2123970/1‑149 (MQ=255)

                                                                                                      tttGTTTTCGATGTCTGTTCCCACCCTCGAGCCAGAAAAGGCCCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                               <  1:3310378/148‑1 (MQ=255)

                                                                                                       ttGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGAAACCGTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                              <  2:1172028/149‑1 (MQ=255)

                                                                                                       ttGTTTTCGATGTCTGTTTCCACCCTCGAGACAGAATAGGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                              <  2:4114420/149‑1 (MQ=255)

                                                                                                          ttttCGATGTCTTTTTCCACCCTCGAGTAATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                           <  2:306169/149‑1 (MQ=255)

                                                                                                          ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGGCACCGGGGGCGCACACACAAAAAAGCCAACAGATTTAACCTCTTTAAGATAGTCCGCCGCCTCCGCAAACCCAGGTTGCCGCCAGTCAGCAACTGCGGGCGCTGTTAGt                                           >  1:739831/1‑149 (MQ=255)

                                                                                                          ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                           <  2:3091879/149‑1 (MQ=255)

                                                                                                             gggttgtgtgTTCCCACCCTCGATTCACAAACGGCGCACCGGGTCGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                        <  1:1166192/145‑1 (MQ=255)

                                                                                                                      ttttCCACCCTCGAGACAGAAAATGCGCAACGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                               <  1:3667733/148‑1 (MQ=255)

                                                                                                                      gTTGCCACCCGCGAGTCAGAAAATGCGCCCCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                               <  2:3817288/149‑1 (MQ=255)

                                                                                                                        ttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                             <  2:2156668/149‑1 (MQ=255)

                                                                                                                        ttCCACCCTCGAGTCAGAAAAGGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                             <  1:3052114/149‑1 (MQ=255)

                                                                                                                        ttCCACCCTCGAGCCAGAAAAGGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                             <  2:2156699/149‑1 (MQ=255)

                                                                                                                        taccccccTCGAGTCACAAAATGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                             <  1:1619909/144‑1 (MQ=255)

                                                                                                                         tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                            <  2:837768/149‑1 (MQ=255)

                                                                                                                          ccACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                           <  2:3888247/149‑1 (MQ=255)

                                                                                                                            ccccTCGAGTCAGAAAAGGCGCACGGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgc                         <  1:1486145/148‑1 (MQ=255)

                                                                                                                            ccccTCGAGTCAGAAAAGGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgc                         <  2:3151350/148‑1 (MQ=255)

                                                                                                                            aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgc                         >  2:3554767/1‑149 (MQ=255)

                                                                                                                              ccTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCACAAACCCTGGTTGCCGCCTGTCAGCATCTGCTGGCGATGGTCGTTGCGGTGATCACGCCAgcgc                       >  2:1883192/1‑149 (MQ=255)

                                                                                                                                tCGAGTCATAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                     <  2:1383661/149‑1 (MQ=255)

                                                                                                                                tCGAGACACAAAAGGCGCACCCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                     <  1:1379226/149‑1 (MQ=255)

                                                                                                                                 cGAGTCAGAAAATGCGCAACCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAt                    <  2:448947/149‑1 (MQ=255)

                                                                                                                                  gAGCCACAAAAGGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAtt                   <  1:850284/149‑1 (MQ=255)

                                                                                                                                     tCATAAAAGGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                <  2:3978857/149‑1 (MQ=255)

                                                                                                                                     tCAGAAAATGCGCCCCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCACACCCGGGTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGGTGCGGTGATCACGCCCGCGCTAGTAAt                >  2:2262885/1‑149 (MQ=255)

                                                                                                                                     tCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                <  2:3978876/149‑1 (MQ=255)

                                                                                                                                       aGAAAATGCGCAACCGGTTCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt              <  1:2673030/149‑1 (MQ=255)

                                                                                                                                          aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           <  2:1816490/149‑1 (MQ=255)

                                                                                                                                          aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           >  2:1000362/1‑149 (MQ=255)

                                                                                                                                            aTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg         >  1:1222643/1‑149 (MQ=255)

                                                                                                                                            aTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg         <  2:2252627/149‑1 (MQ=255)

                                                                                                                                                gccaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc     <  2:920197/146‑1 (MQ=255)

                                                                                                                                                 cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCt    >  1:3272136/1‑149 (MQ=255)

                                                                                                                                                   aCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  2:2542921/1‑149 (MQ=255)

ATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822193‑3822488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`