Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 32,659 C→A 100% R615S (CGC→AGC)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927332,6590CA99.2% 815.8 / ‑2.2 252R615S (CGC→AGC) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (126/123);  total (129/123)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 252 total aligned reads displayed.

CGGCGGCCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGG  >  CP009273/32514‑32804
                                                                                                                                                 |                                                                                                                                                 
     gcCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTAc                                                                                                                                           >  2:3334878/1‑149 (MQ=255)
       ccGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACtt                                                                                                                                         <  2:2260814/149‑1 (MQ=255)
        cGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTc                                                                                                                                        <  1:2665876/149‑1 (MQ=255)
         gAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCg                                                                                                                                       <  1:1014769/149‑1 (MQ=255)
         gAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCg                                                                                                                                       <  1:3134260/149‑1 (MQ=255)
         gAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCg                                                                                                                                       <  2:2124716/149‑1 (MQ=255)
          aaCCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGa                                                                                                                                      >  2:763436/1‑149 (MQ=255)
           aCCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACGGCCTCTACTTCGAg                                                                                                                                     >  2:291599/1‑149 (MQ=255)
                aTCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGt                                                                                                                                <  2:3195030/149‑1 (MQ=255)
                aTCGGTCAGGGTATCGAATTCGACTACTGGTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGt                                                                                                                                <  2:3091864/149‑1 (MQ=255)
                 tCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTa                                                                                                                               <  1:1155918/149‑1 (MQ=255)
                   ggTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAAc                                                                                                                             <  2:3017858/149‑1 (MQ=255)
                     tCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAActc                                                                                                                           <  2:2176888/149‑1 (MQ=255)
                      cAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAActct                                                                                                                          <  1:613801/149‑1 (MQ=255)
                       aGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTg                                                                                                                         <  2:3889744/149‑1 (MQ=255)
                       aGGGGATCGAATTCGACTACGGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCAGGTAACTCTg                                                                                                                         <  2:2245408/149‑1 (MQ=255)
                           tATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAg                                                                                                                     <  1:954918/149‑1 (MQ=255)
                            aTCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGa                                                                                                                    >  1:844634/1‑149 (MQ=255)
                            aTCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGa                                                                                                                    >  1:3080680/1‑149 (MQ=255)
                             tCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGAt                                                                                                                   <  1:1006022/149‑1 (MQ=255)
                               gAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGAtgt                                                                                                                 >  2:2304457/1‑149 (MQ=255)
                                aaTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGAtgtg                                                                                                                <  1:2607470/149‑1 (MQ=255)
                                 aTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGc                                                                                                               <  1:2229132/149‑1 (MQ=255)
                                 aTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGc                                                                                                               <  1:624842/149‑1 (MQ=255)
                                  ttCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCt                                                                                                              <  2:1025519/149‑1 (MQ=255)
                                    cGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTgg                                                                                                            >  1:330292/1‑149 (MQ=255)
                                    cGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTgg                                                                                                            >  1:1330353/1‑149 (MQ=255)
                                     gACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGa                                                                                                           >  2:118577/1‑149 (MQ=255)
                                     gACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGa                                                                                                           >  2:118299/1‑149 (MQ=255)
                                       ctactGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGaaa                                                                                                         <  1:1369498/149‑1 (MQ=255)
                                       ctactGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGaaa                                                                                                         <  2:439776/149‑1 (MQ=255)
                                         actGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATc                                                                                                       <  1:3012313/149‑1 (MQ=255)
                                         actGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATc                                                                                                       >  2:855342/1‑149 (MQ=255)
                                             ttGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGc                                                                                                   >  2:3585161/1‑149 (MQ=255)
                                             gtGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGc                                                                                                   <  1:1791540/148‑1 (MQ=255)
                                                  tACACGCCGCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATc                                                                                              >  2:575803/1‑149 (MQ=255)
                                                   acacGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCg                                                                                             >  1:153961/1‑149 (MQ=255)
                                                   acacGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCg                                                                                             >  2:1265936/1‑149 (MQ=255)
                                                   acacGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCg                                                                                             >  1:153204/1‑149 (MQ=255)
                                                    cacGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga                                                                                            <  2:437359/149‑1 (MQ=255)
                                                      cGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCgaga                                                                                          >  2:1136160/1‑149 (MQ=255)
                                                                gcgcTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAgg                                                                                >  2:429420/1‑149 (MQ=255)
                                                                 cgcTGCTCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACGGTCTCCACCGACTACGACACTTCCGTCAGCCTCTTCTTGGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGc                                                                               <  1:4036011/149‑1 (MQ=255)
                                                                    tGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGtt                                                                            >  2:1498896/1‑149 (MQ=255)
                                                                     gcgcGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGNCAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTa                                                                           >  1:2083793/1‑149 (MQ=255)
                                                                     gcgcGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTa                                                                           >  2:1962537/1‑149 (MQ=255)
                                                                      cgcgAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTAt                                                                          <  1:2774825/149‑1 (MQ=255)
                                                                         gAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGt                                                                       >  2:2741369/1‑149 (MQ=255)
                                                                          aaGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTc                                                                      <  1:4013774/149‑1 (MQ=255)
                                                                          aaGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTc                                                                      >  1:3351590/1‑149 (MQ=255)
                                                                            gACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCa                                                                    <  2:2665773/149‑1 (MQ=255)
                                                                               ggTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTa                                                                 <  2:615069/149‑1 (MQ=255)
                                                                                     gAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTCcggcgg                                                           >  2:3042539/1‑149 (MQ=255)
                                                                                      aaaCCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGt                                                          >  1:1948534/1‑149 (MQ=255)
                                                                                       aaCCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTc                                                         >  1:2866691/1‑149 (MQ=255)
                                                                                        aCCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCa                                                        <  2:3782824/149‑1 (MQ=255)
                                                                                         ccATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAg                                                       >  1:2353691/1‑149 (MQ=255)
                                                                                            ttATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcc                                                    <  1:2455802/149‑1 (MQ=255)
                                                                                            ttATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcc                                                    <  1:385761/149‑1 (MQ=255)
                                                                                            ttATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcc                                                    >  1:2511512/1‑149 (MQ=255)
                                                                                            ttATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcc                                                    >  2:81835/1‑149 (MQ=255)
                                                                                             tATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAccc                                                   <  1:763436/149‑1 (MQ=255)
                                                                                             tATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAccc                                                   <  2:233646/149‑1 (MQ=255)
                                                                                              aTGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcccc                                                  <  2:965360/149‑1 (MQ=255)
                                                                                              aTGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcccc                                                  <  2:2483469/149‑1 (MQ=255)
                                                                                              aTGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcccc                                                  <  1:478119/149‑1 (MQ=255)
                                                                                                ggTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGc                                                <  2:3274651/149‑1 (MQ=255)
                                                                                                    aaCTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGaa                                            >  1:3123766/1‑149 (MQ=255)
                                                                                                      cTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAATCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAAc                                          >  1:1722579/1‑149 (MQ=255)
                                                                                                      cTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAAc                                          <  1:445936/149‑1 (MQ=255)
                                                                                                      cTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAAc                                          <  2:1752942/149‑1 (MQ=255)
                                                                                                       tGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACt                                         >  2:3191319/1‑149 (MQ=255)
                                                                                                        gTAACGCTGAAACCGTCTCCACAGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTg                                        <  2:3461184/149‑1 (MQ=255)
                                                                                                        gTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTg                                        <  2:1920120/149‑1 (MQ=255)
                                                                                                           aCCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcg                                     >  2:1480440/1‑149 (MQ=255)
                                                                                                              cGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcgcgc                                  >  2:2217916/1‑149 (MQ=255)
                                                                                                               ggAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcgcgcg                                 >  1:1961867/1‑149 (MQ=255)
                                                                                                                  aaCCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcgcgcgcgc                              <  1:1848874/149‑1 (MQ=255)
                                                                                                                      gTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGa                          >  1:1584273/1‑149 (MQ=255)
                                                                                                                      gTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGa                          >  1:891054/1‑149 (MQ=255)
                                                                                                                      gTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGa                          <  1:3436314/149‑1 (MQ=255)
                                                                                                                          ccACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgct                      <  1:2815405/149‑1 (MQ=255)
                                                                                                                          ccACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgct                      >  1:2287799/1‑149 (MQ=255)
                                                                                                                            aCCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgctgc                    >  1:186049/1‑149 (MQ=255)
                                                                                                                            aCCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgctgc                    <  2:2771971/149‑1 (MQ=255)
                                                                                                                             ccGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgctgct                   <  1:3684321/149‑1 (MQ=255)
                                                                                                                             ccGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgctgct                   <  2:3638752/149‑1 (MQ=255)
                                                                                                                             ccGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATAGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgctgct                   >  2:3496901/1‑149 (MQ=255)
                                                                                                                              cGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGGTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGctgctg                  >  2:67210/1‑149 (MQ=255)
                                                                                                                               gACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTgg                 <  2:2965323/149‑1 (MQ=255)
                                                                                                                               gACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTGATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTgg                 <  2:2093157/149‑1 (MQ=255)
                                                                                                                                  tACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGt              <  2:1582189/149‑1 (MQ=255)
                                                                                                                                  tACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGt              >  1:3231618/1‑149 (MQ=255)
                                                                                                                                    cGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTAc            <  1:1136160/149‑1 (MQ=255)
                                                                                                                                       cacTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCgg         >  1:3341177/1‑149 (MQ=255)
                                                                                                                                       cacTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGCCGCGCGCGCTGGAAGCTGCTGGCGTACCgg         >  1:3955532/1‑149 (MQ=255)
                                                                                                                                         cTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGtt       <  1:1775760/149‑1 (MQ=255)
                                                                                                                                           tCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTAt     >  2:3694021/1‑149 (MQ=255)
                                                                                                                                           tCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTAt     >  2:3694534/1‑149 (MQ=255)
                                                                                                                                              gACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAgg  >  2:93323/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGGCGGCCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGG  >  CP009273/32514‑32804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑