Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,861,553 C→A 100% G373C (GGC→TGC)  yidR ← DUF3748 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,861,5530CA98.6% 937.7 / 2.8 289G373C (GGC→TGC) yidRDUF3748 family protein
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base A (137/148);  total (141/148)
Fisher's exact test for biased strand distribution p-value = 5.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 294 total aligned reads displayed.

TGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCAC  >  CP009273/3861407‑3861698
                                                                                                                                                  |                                                                                                                                                 
tGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTcgcagc                                                                                                                                                 <  1:3091146/149‑1 (MQ=255)
  ccTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCtt                                                                                                                                               >  2:2893168/1‑149 (MQ=255)
   cTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCttt                                                                                                                                              >  2:249704/1‑149 (MQ=255)
    tGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTg                                                                                                                                             >  2:3012577/1‑149 (MQ=255)
     gATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTgc                                                                                                                                            >  1:686604/1‑149 (MQ=255)
       tAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTgcgc                                                                                                                                          >  2:2604102/1‑149 (MQ=255)
         aCTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCAt                                                                                                                                        <  1:840611/149‑1 (MQ=255)
          cTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATg                                                                                                                                       >  2:1826068/1‑149 (MQ=255)
           tGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATgg                                                                                                                                      >  1:1832542/1‑149 (MQ=255)
            gATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATggg                                                                                                                                     >  1:641769/1‑149 (MQ=255)
             aTTTAGCGATCAGTGTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGc                                                                                                                                    <  2:379799/149‑1 (MQ=255)
                 aGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACaa                                                                                                                                <  2:1600402/149‑1 (MQ=255)
                     aTCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCaa                                                                                                                            <  1:1582717/149‑1 (MQ=255)
                      tCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAt                                                                                                                           <  1:3070912/149‑1 (MQ=255)
                         gTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCg                                                                                                                        >  1:3661871/1‑149 (MQ=255)
                         gTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCg                                                                                                                        >  1:133067/1‑149 (MQ=255)
                          tttCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAGCCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGa                                                                                                                       >  1:2888815/1‑149 (MQ=255)
                            tCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGAtt                                                                                                                     <  2:2310943/149‑1 (MQ=255)
                               gTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATc                                                                                                                  >  1:1751033/1‑149 (MQ=255)
                                 gATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCa                                                                                                                >  1:795825/1‑149 (MQ=255)
                                  aTCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAg                                                                                                               >  1:3228864/1‑149 (MQ=255)
                                    ccACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                                                             <  2:1553607/149‑1 (MQ=255)
                                    ccACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                                                             <  2:2761489/149‑1 (MQ=255)
                                     cacaGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGcac                                                                                                            >  2:1973166/1‑149 (MQ=255)
                                     cacaGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGcac                                                                                                            <  1:1242249/149‑1 (MQ=255)
                                      acaGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGcaca                                                                                                           >  1:2571019/1‑149 (MQ=255)
                                        aGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACaaa                                                                                                         <  1:4065401/149‑1 (MQ=255)
                                          cTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGc                                                                                                       >  1:732685/1‑149 (MQ=255)
                                           tGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGcc                                                                                                      >  1:91062/1‑149 (MQ=255)
                                              ccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCaa                                                                                                   <  1:992868/149‑1 (MQ=255)
                                              ccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCaa                                                                                                   <  2:925019/149‑1 (MQ=255)
                                              ccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCaa                                                                                                   <  1:2018374/149‑1 (MQ=255)
                                                    tccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATtc                                                                                             <  2:686604/149‑1 (MQ=255)
                                                       atccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTcc                                                                                          >  2:3520962/1‑149 (MQ=255)
                                                       atccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTcc                                                                                          >  2:2642833/1‑149 (MQ=255)
                                                            cGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACg                                                                                     <  1:3710543/149‑1 (MQ=255)
                                                              ccagccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGa                                                                                   <  1:2626392/149‑1 (MQ=255)
                                                                 gccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGc                                                                                <  1:358264/149‑1 (MQ=255)
                                                                   caCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCa                                                                              <  1:3368144/149‑1 (MQ=255)
                                                                     cTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGt                                                                            <  2:2348689/149‑1 (MQ=255)
                                                                     cTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGt                                                                            >  1:2295517/1‑149 (MQ=255)
                                                                     cTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGt                                                                            >  1:2497648/1‑149 (MQ=255)
                                                                       gACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTa                                                                          <  1:3932433/149‑1 (MQ=255)
                                                                        aCCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTaa                                                                         >  1:921286/1‑149 (MQ=255)
                                                                         ccATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTaaa                                                                        <  2:2103298/149‑1 (MQ=255)
                                                                          cATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAAt                                                                       <  2:2108893/149‑1 (MQ=255)
                                                                           aTCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATg                                                                      >  1:1008183/1‑149 (MQ=255)
                                                                           aTCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATg                                                                      >  1:1447946/1‑149 (MQ=255)
                                                                           aTCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATg                                                                      <  2:166826/149‑1 (MQ=255)
                                                                           aTCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATg                                                                      <  2:510861/149‑1 (MQ=255)
                                                                              cGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAg                                                                   >  2:1201548/1‑149 (MQ=255)
                                                                                    gaagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGaa                                                             <  2:3500114/149‑1 (MQ=255)
                                                                                     aagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAat                                                            <  1:3805318/149‑1 (MQ=255)
                                                                                     aagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAat                                                            <  1:2152425/149‑1 (MQ=255)
                                                                                     aagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAat                                                            <  2:3969254/149‑1 (MQ=255)
                                                                                      agaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAata                                                           <  2:3670435/149‑1 (MQ=255)
                                                                                       gaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAatat                                                          <  2:3361054/149‑1 (MQ=255)
                                                                                       gaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAatat                                                          <  2:613421/149‑1 (MQ=255)
                                                                                        aCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATc                                                         <  2:2469440/149‑1 (MQ=255)
                                                                                         ccACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATcc                                                        <  1:2660702/149‑1 (MQ=255)
                                                                                          cACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCg                                                       <  1:3522306/149‑1 (MQ=255)
                                                                                            cGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGtt                                                     <  1:1675236/149‑1 (MQ=255)
                                                                                                gTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGt                                                 <  1:3496271/149‑1 (MQ=255)
                                                                                                gTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGt                                                 <  2:2162617/149‑1 (MQ=255)
                                                                                                 tCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGtt                                                >  2:2411500/1‑149 (MQ=255)
                                                                                                   cGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTAt                                              <  2:2856998/149‑1 (MQ=255)
                                                                                                   cGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAAGACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTAt                                              <  2:3693578/149‑1 (MQ=255)
                                                                                                     cAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGANTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATgg                                            >  1:2080924/1‑149 (MQ=255)
                                                                                                        aaGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGtt                                         >  1:1595123/1‑149 (MQ=255)
                                                                                                          ggTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTaa                                       <  2:3250848/149‑1 (MQ=255)
                                                                                                           gTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAGGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAAc                                      >  1:4112916/1‑149 (MQ=255)
                                                                                                           gTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAAc                                      >  2:1120850/1‑149 (MQ=255)
                                                                                                             gAATTGGCGTGGTGGTCGGTTAAATACTCAAGCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTACATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTg                                    >  1:2343907/1‑149 (MQ=255)
                                                                                                                  ggCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGcgcg                               >  2:193211/1‑149 (MQ=255)
                                                                                                                    cGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGc                             <  1:1124852/149‑1 (MQ=255)
                                                                                                                      tggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTc                           >  2:841117/1‑149 (MQ=255)
                                                                                                                       ggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTcg                          >  1:1491238/1‑149 (MQ=255)
                                                                                                                             cGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGccc                    <  1:535362/149‑1 (MQ=255)
                                                                                                                             cGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGccc                    <  1:430995/149‑1 (MQ=255)
                                                                                                                             cGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGccc                    <  1:943195/149‑1 (MQ=255)
                                                                                                                             cGGTTAAAGACTCACCCTCGCAGCTTTGCGCATGGGCACAAGCACTTCGAGTATCCAGCACAAAGCCCAACCCTTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGccc                    <  1:1093408/149‑1 (MQ=255)
                                                                                                                              ggTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCt                   <  1:2909989/149‑1 (MQ=255)
                                                                                                                               gTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtg                  >  1:3005702/1‑149 (MQ=255)
                                                                                                                               gTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtg                  >  1:1037422/1‑149 (MQ=255)
                                                                                                                               gTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtg                  >  2:3284398/1‑149 (MQ=255)
                                                                                                                               gTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtg                  >  1:108994/1‑149 (MQ=255)
                                                                                                                               gTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTGTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtg                  >  2:2088572/1‑149 (MQ=255)
                                                                                                                                ttAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtgt                 >  2:527340/1‑149 (MQ=255)
                                                                                                                                ttAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtgt                 >  1:1423458/1‑149 (MQ=255)
                                                                                                                                ttAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtgt                 >  1:4005266/1‑149 (MQ=255)
                                                                                                                                ttAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTGTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtgt                 >  2:1024625/1‑149 (MQ=255)
                                                                                                                                 tAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtgtg                <  1:2799522/149‑1 (MQ=255)
                                                                                                                                 tAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtgtg                >  2:1382771/1‑149 (MQ=255)
                                                                                                                                  aaaTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTgg               <  2:795842/149‑1 (MQ=255)
                                                                                                                                     tACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACTGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCga            <  1:86367/149‑1 (MQ=255)
                                                                                                                                     tACTCAACCTCGCAGCTGTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAACGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCga            <  2:4355/149‑1 (MQ=255)
                                                                                                                                          aaCCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATaa       <  2:3088664/149‑1 (MQ=255)
                                                                                                                                            ccTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGc     >  1:2079474/1‑149 (MQ=255)
                                                                                                                                            ccTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGc     <  1:3551724/149‑1 (MQ=255)
                                                                                                                                              tCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCca   <  2:3228864/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCAC  >  CP009273/3861407‑3861698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑