Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,863,438 G→T 100% M234I (ATG→ATT)  cbrA → colicin M resistance protein; FAD‑binding protein, putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,863,4380GT97.1% 764.0 / 10.8 243M234I (ATG→ATT) cbrAcolicin M resistance protein; FAD‑binding protein, putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (6/1);  new base T (133/103);  total (139/104)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 243 total aligned reads displayed.

TCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGC  >  CP009273/3863291‑3863583
                                                                                                                                                   |                                                                                                                                                 
tCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGa                                                                                                                                                  >  1:411197/1‑149 (MQ=255)
 ccGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAg                                                                                                                                                 <  1:3083620/149‑1 (MQ=255)
    gTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAgcgc                                                                                                                                              >  1:2182754/1‑149 (MQ=255)
           tCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAg                                                                                                                                       >  1:448454/1‑149 (MQ=255)
           tCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAg                                                                                                                                       >  1:2831062/1‑149 (MQ=255)
           tCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAg                                                                                                                                       >  1:2668669/1‑149 (MQ=255)
             tACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGtt                                                                                                                                     >  2:1163337/1‑149 (MQ=255)
               cTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTcc                                                                                                                                   <  1:1309945/149‑1 (MQ=255)
               cTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTcc                                                                                                                                   <  1:3088526/149‑1 (MQ=255)
               cTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTcc                                                                                                                                   <  2:167014/149‑1 (MQ=255)
                  cTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGt                                                                                                                                >  2:2476518/1‑149 (MQ=255)
                  cTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGt                                                                                                                                >  1:238641/1‑149 (MQ=255)
                     cATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTg                                                                                                                             >  1:3654445/1‑149 (MQ=255)
                     cATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTg                                                                                                                             <  2:3277544/149‑1 (MQ=255)
                      aTCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTgg                                                                                                                            >  1:3896248/1‑149 (MQ=255)
                      aTCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTgg                                                                                                                            >  1:144714/1‑149 (MQ=255)
                       tCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGt                                                                                                                           <  2:1762797/149‑1 (MQ=255)
                        cTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTa                                                                                                                          <  2:3654445/149‑1 (MQ=255)
                        cTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTa                                                                                                                          >  1:20481/1‑149 (MQ=255)
                           tGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGa                                                                                                                       <  2:562038/149‑1 (MQ=255)
                            gATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGAc                                                                                                                      <  1:1339041/149‑1 (MQ=255)
                             aTAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACg                                                                                                                     <  2:3002854/149‑1 (MQ=255)
                             aTAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACg                                                                                                                     >  2:2940848/1‑149 (MQ=255)
                              tAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACgg                                                                                                                    >  2:1704910/1‑149 (MQ=255)
                                aTTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTg                                                                                                                  <  1:3725688/149‑1 (MQ=255)
                                aTTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTGCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTg                                                                                                                  >  2:1839243/1‑149 (MQ=255)
                                 ttCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTATCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGa                                                                                                                 >  1:2996257/1‑149 (MQ=255)
                                    gATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                                                                                              >  2:2965456/1‑149 (MQ=255)
                                    gATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                                                                                              <  1:3728052/149‑1 (MQ=255)
                                    gATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                                                                                              <  2:3402050/149‑1 (MQ=255)
                                    gATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                                                                                              >  2:393243/1‑149 (MQ=255)
                                    gATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                                                                                              >  1:2526162/1‑149 (MQ=255)
                                       aactaactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCg                                                                                                           >  1:1733242/1‑149 (MQ=255)
                                       aactaactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCg                                                                                                           >  1:2486839/1‑149 (MQ=255)
                                       aactaactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCg                                                                                                           >  1:3778280/1‑149 (MQ=255)
                                         ctaactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGaa                                                                                                         >  1:836354/1‑149 (MQ=255)
                                               gTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGc                                                                                                   >  2:1465036/1‑149 (MQ=255)
                                               gTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGc                                                                                                   <  2:873382/149‑1 (MQ=255)
                                                 tATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCAc                                                                                                 <  1:3510832/149‑1 (MQ=255)
                                                 tATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCAc                                                                                                 <  1:2278126/149‑1 (MQ=255)
                                                  aTTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACg                                                                                                <  1:1163337/149‑1 (MQ=255)
                                                   ttCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACgg                                                                                               <  2:1296727/149‑1 (MQ=255)
                                                   ttCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACgg                                                                                               <  2:1055713/149‑1 (MQ=255)
                                                   ttCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACgg                                                                                               >  1:3263834/1‑149 (MQ=255)
                                                    tCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGt                                                                                              >  1:595057/1‑149 (MQ=255)
                                                        ggAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTg                                                                                          >  1:1961983/1‑149 (MQ=255)
                                                          aGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCCGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGtt                                                                                        >  1:1628857/1‑149 (MQ=255)
                                                            tATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTc                                                                                      >  2:1496004/1‑149 (MQ=255)
                                                            tATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTc                                                                                      >  2:3794733/1‑149 (MQ=255)
                                                            tATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTc                                                                                      >  2:3098619/1‑149 (MQ=255)
                                                            tATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTc                                                                                      >  2:3335952/1‑149 (MQ=255)
                                                            tATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTc                                                                                      <  1:2892400/149‑1 (MQ=255)
                                                             aTCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTcc                                                                                     >  2:1992916/1‑149 (MQ=255)
                                                             aTCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTcc                                                                                     >  1:1670627/1‑149 (MQ=255)
                                                             aTCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTcc                                                                                     <  2:2532168/149‑1 (MQ=255)
                                                              tCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTccc                                                                                    <  1:4006468/149‑1 (MQ=255)
                                                               cAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCt                                                                                   <  2:3313640/149‑1 (MQ=255)
                                                               cAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAACGCACGGTGCTGTTTCCCt                                                                                   <  2:2809058/149‑1 (MQ=255)
                                                                 gCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTcg                                                                                 >  2:1186244/1‑149 (MQ=255)
                                                                   aaaGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTcgcg                                                                               >  2:4014096/1‑149 (MQ=255)
                                                                     aGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCt                                                                             >  2:1909191/1‑149 (MQ=255)
                                                                     aGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCt                                                                             >  1:1659673/1‑149 (MQ=255)
                                                                        cGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGc                                                                          <  2:2157238/149‑1 (MQ=255)
                                                                              ttttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAtt                                                                    >  1:1996351/1‑149 (MQ=255)
                                                                              ttttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAtt                                                                    >  1:885079/1‑149 (MQ=255)
                                                                              ttttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAtt                                                                    >  1:3295933/1‑149 (MQ=255)
                                                                               tttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAttt                                                                   >  1:2165316/1‑149 (MQ=255)
                                                                                ttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAtttt                                                                  <  2:791671/149‑1 (MQ=255)
                                                                                 tATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTg                                                                 >  2:3597258/1‑149 (MQ=255)
                                                                                  aTCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGt                                                                <  2:3852496/149‑1 (MQ=255)
                                                                                        ggCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCgg                                                          >  1:516330/1‑149 (MQ=255)
                                                                                        ggCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAAGGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCgg                                                          <  2:902287/149‑1 (MQ=255)
                                                                                         gCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGt                                                         <  1:1496004/149‑1 (MQ=255)
                                                                                             tGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAgg                                                     <  1:3432897/149‑1 (MQ=255)
                                                                                                 tATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACaa                                                 <  2:2690489/149‑1 (MQ=255)
                                                                                                 tATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACaa                                                 >  2:2322383/1‑149 (MQ=255)
                                                                                                 tATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACaa                                                 <  2:3778280/149‑1 (MQ=255)
                                                                                                   tCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACg                                               >  2:2451928/1‑149 (MQ=255)
                                                                                                     cAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGcc                                             <  1:1819502/149‑1 (MQ=255)
                                                                                                     cAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGcc                                             <  1:629955/149‑1 (MQ=255)
                                                                                                      aaTGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCt                                            >  1:1277807/1‑149 (MQ=255)
                                                                                                      aaTGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCt                                            >  2:793680/1‑149 (MQ=255)
                                                                                                      aaTGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCt                                            <  1:3631449/149‑1 (MQ=255)
                                                                                                       aTGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCtt                                           <  2:3708756/149‑1 (MQ=255)
                                                                                                         gAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTc                                         <  1:2440955/149‑1 (MQ=255)
                                                                                                         gAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTc                                         <  2:801846/149‑1 (MQ=255)
                                                                                                           aaGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTg                                       >  1:3690388/1‑149 (MQ=255)
                                                                                                            aGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGa                                      >  1:3802314/1‑149 (MQ=255)
                                                                                                                ggTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTgg                                  <  1:771686/149‑1 (MQ=255)
                                                                                                                 gTCAGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGt                                 >  1:1219409/1‑149 (MQ=255)
                                                                                                                    aGACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGaa                              <  2:1151444/149‑1 (MQ=255)
                                                                                                                     gACGCGTTTCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAg                             >  2:437938/1‑149 (MQ=255)
                                                                                                                           tttCACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGcggcgg                       <  1:263509/149‑1 (MQ=255)
                                                                                                                              cACGACGCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGAt                    >  2:1424157/1‑149 (MQ=255)
                                                                                                                               acgacgCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGAtt                   <  2:823414/149‑1 (MQ=255)
                                                                                                                               acgacgCTGAAAGAGAAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTGTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGAtt                   <  2:2747858/149‑1 (MQ=255)
                                                                                                                                           gagaAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAg       >  2:2852972/1‑149 (MQ=255)
                                                                                                                                             gaAAATTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCt     >  1:660698/1‑149 (MQ=255)
                                                                                                                                              aaaaTTAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTc    >  2:2031268/1‑149 (MQ=255)
                                                                                                                                                aaTTAGCGCCTGTCAGTTCCAGTTTGGTAAGACGGGGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGc  <  2:3008438/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCCGGTGCCGTTCTACTCCTGCATCTTTGATAATTCGATAACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGATTTTGTCTGCGGTAAGGACAACGCCTTTCTGATTGGTGAAGCGGCGGGATTTATCAGCGCCAGCTCGC  >  CP009273/3863291‑3863583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑