Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,865,120 C→A 100% intergenic (‑30/+90) dgoT ← / ← dgoD D‑galactonate transporter/galactonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,865,1200CA98.0% 790.6 / 5.5 247intergenic (‑30/+90)dgoT/dgoDD‑galactonate transporter/galactonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (103/139);  total (105/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 251 total aligned reads displayed.

GGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTCCGG  >  CP009273/3864974‑3865266
                                                                                                                                                  |                                                                                                                                                  
    gCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGcc                                                                                                                                              >  1:2930650/1‑149 (MQ=255)
             aGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCCTAGAGAAGCCGCTCACCAt                                                                                                                                     >  2:434477/1‑149 (MQ=255)
               gTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGc                                                                                                                                   <  1:4089046/149‑1 (MQ=255)
               gTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGc                                                                                                                                   >  1:2655407/1‑149 (MQ=255)
                 tGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGccc                                                                                                                                 <  2:3767799/149‑1 (MQ=255)
                  ggCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGATCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCt                                                                                                                                >  2:1799265/1‑149 (MQ=255)
                  ggCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCt                                                                                                                                >  2:2608270/1‑149 (MQ=255)
                       ggTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAAt                                                                                                                           >  1:3293769/1‑149 (MQ=255)
                       ggTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAAt                                                                                                                           <  2:2878553/149‑1 (MQ=255)
                         tCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAAttt                                                                                                                         <  2:843134/149‑1 (MQ=255)
                          cGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTa                                                                                                                        <  1:1248292/149‑1 (MQ=255)
                          cGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTa                                                                                                                        <  1:1657983/149‑1 (MQ=255)
                            aCATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACa                                                                                                                      >  2:2941848/1‑149 (MQ=255)
                             aaTAAAAAATTACTACCTTAATAAATATAATCACCTGCTTCATATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGTAATATCAATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACag                                                                                                                     <  1:3828291/148‑1 (MQ=255)
                              aTAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACaga                                                                                                                    <  1:3327876/149‑1 (MQ=255)
                              aTAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACaga                                                                                                                    <  2:3772649/149‑1 (MQ=255)
                              aTAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACaga                                                                                                                    >  2:3729451/1‑149 (MQ=255)
                              aTAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACaga                                                                                                                    <  1:2186366/149‑1 (MQ=255)
                               tAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACagag                                                                                                                   >  1:1846843/1‑149 (MQ=255)
                                aaCAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCCTAGCTAGCTTTCTCCAGAGTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAgg                                                                                                                  <  1:4034222/149‑1 (MQ=255)
                                aaCAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAgg                                                                                                                  >  1:846189/1‑149 (MQ=255)
                                 aCAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAggg                                                                                                                 <  2:3395631/149‑1 (MQ=255)
                                  cAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGt                                                                                                                <  2:1451471/149‑1 (MQ=255)
                                    aaTGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTta                                                                                                              <  1:2493749/149‑1 (MQ=255)
                                    aaTGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTta                                                                                                              <  1:3161843/149‑1 (MQ=255)
                                     aTGACTCCCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTtat                                                                                                             <  1:3877725/149‑1 (MQ=255)
                                     aTGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTtat                                                                                                             <  2:1925338/149‑1 (MQ=255)
                                      tGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTtata                                                                                                            <  2:1163561/149‑1 (MQ=255)
                                           aCCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATAttttt                                                                                                       <  2:232907/149‑1 (MQ=255)
                                            ccGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTACTGTACAGAGGGTTATATTTTTg                                                                                                      >  2:2854249/1‑149 (MQ=255)
                                            ccGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTg                                                                                                      <  2:1994366/149‑1 (MQ=255)
                                              gTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTGATATTTTTGTa                                                                                                    >  2:1137431/1‑149 (MQ=255)
                                               taataaAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTAt                                                                                                   >  2:1295613/1‑149 (MQ=255)
                                                aataaAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTAtt                                                                                                  <  2:3499187/149‑1 (MQ=255)
                                                aataaAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGGTATATTTTTGTAtt                                                                                                  >  1:3480847/1‑149 (MQ=255)
                                                 ataaAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTg                                                                                                 >  1:2544652/1‑149 (MQ=255)
                                                  taaAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGc                                                                                                >  2:3337389/1‑149 (MQ=255)
                                                        tcatcaCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGtttt                                                                                          >  2:3878636/1‑149 (MQ=255)
                                                        tcatcaCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGtttt                                                                                          >  1:1036747/1‑149 (MQ=255)
                                                          atcaCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAg                                                                                        <  1:1359740/149‑1 (MQ=255)
                                                           tcaCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGt                                                                                       <  1:1304525/149‑1 (MQ=255)
                                                           tcaCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGt                                                                                       >  1:843891/1‑149 (MQ=255)
                                                           tcaCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGt                                                                                       <  2:129245/149‑1 (MQ=255)
                                                           tcaCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGt                                                                                       <  1:2836421/149‑1 (MQ=255)
                                                            caCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTg                                                                                      <  2:1041074/149‑1 (MQ=255)
                                                               cAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGccc                                                                                   <  1:1987357/149‑1 (MQ=255)
                                                                aGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCg                                                                                  <  1:3688276/149‑1 (MQ=255)
                                                                 gCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGa                                                                                 >  1:161525/1‑149 (MQ=255)
                                                                   gTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATg                                                                               <  1:3380964/149‑1 (MQ=255)
                                                                    tCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGa                                                                              <  2:477316/149‑1 (MQ=255)
                                                                    tCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGa                                                                              >  1:1616747/1‑149 (MQ=255)
                                                                    tCAGATAACGCCGACGCCCCGGCTTTGCTGCATGAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGa                                                                              <  2:3553191/149‑1 (MQ=255)
                                                                      aGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAgg                                                                            <  2:940093/149‑1 (MQ=255)
                                                                      aGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAgg                                                                            <  1:2472302/149‑1 (MQ=255)
                                                                       gATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCAAAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATATTTGTATTGCTGTTTTAGTGCCCGATGAggg                                                                           >  1:680287/1‑149 (MQ=255)
                                                                           aCGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCtt                                                                       <  2:2452909/149‑1 (MQ=255)
                                                                           aCGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCtt                                                                       <  1:3178631/149‑1 (MQ=255)
                                                                             gCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTAc                                                                     >  1:3110424/1‑149 (MQ=255)
                                                                             gCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTAc                                                                     <  2:1630065/149‑1 (MQ=255)
                                                                              ccGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACg                                                                    <  2:2617305/149‑1 (MQ=255)
                                                                              ccGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACg                                                                    >  1:2304531/1‑149 (MQ=255)
                                                                                gACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTg                                                                  >  2:170818/1‑149 (MQ=255)
                                                                                  cGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGc                                                                <  1:2375319/149‑1 (MQ=255)
                                                                                  cGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGc                                                                <  2:778410/149‑1 (MQ=255)
                                                                                      ccGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTGTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGa                                                            <  1:492912/149‑1 (MQ=255)
                                                                                        ggCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAAt                                                          >  2:3120428/1‑149 (MQ=255)
                                                                                        ggCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAAt                                                          <  1:1821256/149‑1 (MQ=255)
                                                                                               ctgcATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCAct                                                   >  2:1259713/1‑149 (MQ=255)
                                                                                                tgcATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCActc                                                  <  1:1920160/149‑1 (MQ=255)
                                                                                                      aaCGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTAc                                            <  2:2760190/149‑1 (MQ=255)
                                                                                                        cGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGc                                          <  1:1104113/149‑1 (MQ=255)
                                                                                                        cGGGAATATCCATAGCGAGCTGTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGc                                          <  1:1185891/149‑1 (MQ=255)
                                                                                                         gggAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCt                                         >  1:1242619/1‑149 (MQ=255)
                                                                                                          ggAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTg                                        >  1:751745/1‑149 (MQ=255)
                                                                                                          ggAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTg                                        >  1:2812769/1‑149 (MQ=255)
                                                                                                           gAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGt                                       <  2:2072626/149‑1 (MQ=255)
                                                                                                             atatCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTa                                     <  2:3698832/149‑1 (MQ=255)
                                                                                                              tatCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTAt                                    <  2:2655137/149‑1 (MQ=255)
                                                                                                              tatCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTAt                                    <  1:910799/149‑1 (MQ=255)
                                                                                                                   aTAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCa                               >  1:1009937/1‑149 (MQ=255)
                                                                                                                   aTAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCa                               <  2:4056856/149‑1 (MQ=255)
                                                                                                                    tAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCAt                              >  1:3196278/1‑149 (MQ=255)
                                                                                                                     aGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATg                             >  1:2575832/1‑149 (MQ=255)
                                                                                                                      gCGAGCTGTCTCCAGATTTTGGGCATAGAGCAGCCGCTCTCCATGCGCTGTATTTTACAGAGTGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGa                            <  1:1513267/149‑1 (MQ=255)
                                                                                                                       cGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGAc                           <  2:1059035/149‑1 (MQ=255)
                                                                                                                         aGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGc                         >  2:2904883/1‑149 (MQ=255)
                                                                                                                          gCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGcc                        >  1:660727/1‑149 (MQ=255)
                                                                                                                          gCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGcc                        >  2:2638883/1‑149 (MQ=255)
                                                                                                                              tctcCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCagag                    <  1:2111772/149‑1 (MQ=255)
                                                                                                                               ctcCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGc                   <  2:1346297/149‑1 (MQ=255)
                                                                                                                                tcCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCg                  <  2:3976769/149‑1 (MQ=255)
                                                                                                                                    gATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGAtt              <  1:3720491/149‑1 (MQ=255)
                                                                                                                                    gATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGAtt              <  1:2444044/149‑1 (MQ=255)
                                                                                                                                    gATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGAtt              <  2:2494143/149‑1 (MQ=255)
                                                                                                                                      ttttGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTAc            <  1:2783756/149‑1 (MQ=255)
                                                                                                                                      ttttGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTAc            <  1:4055767/149‑1 (MQ=255)
                                                                                                                                              aTAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTcc    >  2:185752/1‑149 (MQ=255)
                                                                                                                                              aTAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTcc    >  2:2296196/1‑149 (MQ=255)
                                                                                                                                               tAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTCCg   >  2:4036275/1‑149 (MQ=255)
                                                                                                                                               tAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTCCg   >  2:1320940/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTCCGG  >  CP009273/3864974‑3865266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑