Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,872,006 T→A 100% S492C (AGC→TGC)  gyrB ← DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,872,0060TA96.6% 778.6 / 11.6 238S492C (AGC→TGC) gyrBDNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base T (2/6);  new base A (116/114);  total (118/120)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 245 total aligned reads displayed.

GCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGA  >  CP009273/3871860‑3872151
                                                                                                                                                  |                                                                                                                                                 
gCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCagt                                                                                                                                                 <  2:774420/149‑1 (MQ=255)
gCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCagt                                                                                                                                                 <  1:1655122/149‑1 (MQ=255)
 ccTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCagtg                                                                                                                                                >  1:1270433/1‑149 (MQ=255)
  cTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGa                                                                                                                                               >  1:959117/1‑149 (MQ=255)
  cTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGa                                                                                                                                               <  1:1411578/149‑1 (MQ=255)
    ttcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATa                                                                                                                                             >  2:847353/1‑149 (MQ=255)
     tcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATaa                                                                                                                                            <  2:2197538/149‑1 (MQ=255)
     tcttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATaa                                                                                                                                            <  2:2197459/149‑1 (MQ=255)
      cttcACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAAc                                                                                                                                           >  2:3695952/1‑149 (MQ=255)
            tttGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGtt                                                                                                                                     >  1:584406/1‑149 (MQ=255)
             ttGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGttt                                                                                                                                    >  2:549616/1‑149 (MQ=255)
                tACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTc                                                                                                                                 <  1:395252/149‑1 (MQ=255)
                 aCAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTcc                                                                                                                                >  1:2642896/1‑149 (MQ=255)
                 aCAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTcc                                                                                                                                >  1:505184/1‑149 (MQ=255)
                  cAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCg                                                                                                                               >  2:1270881/1‑149 (MQ=255)
                   aGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCgg                                                                                                                              <  1:3455724/149‑1 (MQ=255)
                    gcggcgGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCggg                                                                                                                             >  1:1144340/1‑149 (MQ=255)
                    gcggcgGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCggg                                                                                                                             >  1:3571847/1‑149 (MQ=255)
                               gCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTc                                                                                                                  <  1:3905772/149‑1 (MQ=255)
                               gCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTc                                                                                                                  <  1:3901395/149‑1 (MQ=255)
                               gCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTc                                                                                                                  <  1:2992894/149‑1 (MQ=255)
                                 gATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAc                                                                                                                <  2:363937/149‑1 (MQ=255)
                                       gACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGa                                                                                                          >  1:3915964/1‑149 (MQ=255)
                                          gTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATAc                                                                                                       <  2:3147355/149‑1 (MQ=255)
                                              ccGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAATGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACcaca                                                                                                   <  2:3130241/149‑1 (MQ=255)
                                                gcgTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGc                                                                                                 >  2:73844/1‑149 (MQ=255)
                                                   ttCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCaa                                                                                              >  1:2857461/1‑149 (MQ=255)
                                                   ttCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCaa                                                                                              <  2:406096/149‑1 (MQ=255)
                                                    tCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAg                                                                                             >  2:1109223/1‑149 (MQ=255)
                                                    tCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAg                                                                                             <  2:3382014/149‑1 (MQ=255)
                                                         gATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCgg                                                                                        <  2:2075419/149‑1 (MQ=255)
                                                         gATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCgg                                                                                        >  1:246452/1‑149 (MQ=255)
                                                          atgtCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGt                                                                                       <  2:2560607/146‑1 (MQ=255)
                                                          aTTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGt                                                                                       >  1:2027818/1‑149 (MQ=255)
                                                          aTTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGt                                                                                       >  1:946/1‑149 (MQ=255)
                                                           tttCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTg                                                                                      >  1:1687675/1‑149 (MQ=255)
                                                                ggCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAg                                                                                 <  1:1023087/149‑1 (MQ=255)
                                                                  cATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCg                                                                               <  2:2159492/149‑1 (MQ=255)
                                                                    tCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTc                                                                             >  2:204914/1‑149 (MQ=255)
                                                                    tCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTc                                                                             >  2:1177061/1‑149 (MQ=255)
                                                                    tCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTc                                                                             <  1:472203/149‑1 (MQ=255)
                                                                     cTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCg                                                                            >  2:121608/1‑149 (MQ=255)
                                                                      tGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGc                                                                           >  1:3597630/1‑149 (MQ=255)
                                                                      tGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGc                                                                           <  2:3282922/149‑1 (MQ=255)
                                                                       gACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGcc                                                                          >  1:1486929/1‑149 (MQ=255)
                                                                          gATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACt                                                                       >  2:1579878/1‑149 (MQ=255)
                                                                          gATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACt                                                                       >  1:2560722/1‑149 (MQ=255)
                                                                          gATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACt                                                                       >  2:3671108/1‑149 (MQ=255)
                                                                                agaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTga                                                                 <  1:4102974/149‑1 (MQ=255)
                                                                                 gaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTgag                                                                >  2:506293/1‑149 (MQ=255)
                                                                                 gaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTgag                                                                >  2:472664/1‑149 (MQ=255)
                                                                                      tCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagag                                                           >  1:2900861/1‑149 (MQ=255)
                                                                                       cAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagaga                                                          <  1:2950517/149‑1 (MQ=255)
                                                                                       cAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagaga                                                          >  1:262535/1‑149 (MQ=255)
                                                                                       cAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagaga                                                          >  2:396575/1‑149 (MQ=255)
                                                                                        aaCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagagag                                                         >  1:2054821/1‑149 (MQ=255)
                                                                                           agcagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCTGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCAt                                                      <  2:2916678/149‑1 (MQ=255)
                                                                                            gcagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATc                                                     <  2:566746/149‑1 (MQ=255)
                                                                                              agcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCtt                                                   <  2:3655018/149‑1 (MQ=255)
                                                                                              agcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCtt                                                   <  2:599167/149‑1 (MQ=255)
                                                                                                cGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                                 <  1:1177067/149‑1 (MQ=255)
                                                                                                cGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                                 <  1:1177060/149‑1 (MQ=255)
                                                                                                cGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                                 <  2:245149/149‑1 (MQ=255)
                                                                                                cGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                                 <  2:1469091/149‑1 (MQ=255)
                                                                                                    cGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGaa                                             <  1:2116398/149‑1 (MQ=255)
                                                                                                        tgtgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgc                                         <  1:3177184/149‑1 (MQ=255)
                                                                                                          tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgcgc                                       <  2:1360109/149‑1 (MQ=255)
                                                                                                              aGCCGTCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtc                                   <  1:311175/149‑1 (MQ=255)
                                                                                                                  gTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGa                               <  2:125042/149‑1 (MQ=255)
                                                                                                                   tCGACGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGAc                              >  2:1219848/1‑149 (MQ=255)
                                                                                                                      aCGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGtt                           <  1:1035590/149‑1 (MQ=255)
                                                                                                                       cGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTg                          <  2:2923038/149‑1 (MQ=255)
                                                                                                                       cGTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTg                          <  2:1123957/149‑1 (MQ=255)
                                                                                                                        gTCCGCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGCTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGa                         <  1:80941/149‑1 (MQ=255)
                                                                                                                            gCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                     >  1:2686736/1‑149 (MQ=255)
                                                                                                                            gCATCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                     <  2:2482009/149‑1 (MQ=255)
                                                                                                                              aTCGGTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAttt                   >  1:3990619/1‑149 (MQ=255)
                                                                                                                                 ggTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAc                <  2:1081111/149‑1 (MQ=255)
                                                                                                                                 ggTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAc                <  2:4075470/149‑1 (MQ=255)
                                                                                                                                 ggTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAc                >  1:135229/1‑149 (MQ=255)
                                                                                                                                 ggTCATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAc                >  1:2211705/1‑149 (MQ=255)
                                                                                                                                    cATGATGATGATGCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCt             <  2:3435439/149‑1 (MQ=255)
                                                                                                                                     atgatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCtt            >  1:35060/1‑149 (MQ=255)
                                                                                                                                     atgatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCtt            >  2:1410402/1‑149 (MQ=255)
                                                                                                                                     atgatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCtt            >  2:3866900/1‑149 (MQ=255)
                                                                                                                                      tgatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTc           <  1:1633884/149‑1 (MQ=255)
                                                                                                                                      tgatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTc           >  1:1159051/1‑149 (MQ=255)
                                                                                                                                      tgatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTc           >  1:1159953/1‑149 (MQ=255)
                                                                                                                                      tgatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTc           <  1:3678496/149‑1 (MQ=255)
                                                                                                                                       gatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCa          <  1:2830160/149‑1 (MQ=255)
                                                                                                                                       gatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCa          >  2:1968884/1‑149 (MQ=255)
                                                                                                                                       gatgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCa          >  2:1968910/1‑149 (MQ=255)
                                                                                                                                         tgatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGc        >  1:4044901/1‑149 (MQ=255)
                                                                                                                                          gatgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCg       >  1:3228505/1‑149 (MQ=255)
                                                                                                                                           atgatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgg      >  2:2232281/1‑149 (MQ=255)
                                                                                                                                            tgatgCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGc     <  2:1649509/149‑1 (MQ=255)
                                                                                                                                            tgatgCAGTGATAACGCAGTTTGTCCGGGTAGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgac     <  2:2155575/149‑3 (MQ=255)
                                                                                                                                             gatgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCa    <  2:2649878/149‑1 (MQ=255)
                                                                                                                                              atgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAg   <  2:1171245/149‑1 (MQ=255)
                                                                                                                                               tgCAGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGa  >  2:3777042/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGA  >  CP009273/3871860‑3872151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑