Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,901,415 C→A 100% P104P (CCG→CCT)  pstB ← phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,901,4150CA97.9% 759.4 / 5.8 239P104P (CCG→CCT) pstBphosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base A (108/126);  total (111/128)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 239 total aligned reads displayed.

GAGTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTG  >  CP009273/3901269‑3901559
                                                                                                                                                  |                                                                                                                                                
gagTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAgg                                                                                                                                                >  1:2548550/1‑149 (MQ=255)
 agTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGa                                                                                                                                               <  1:2673193/149‑1 (MQ=255)
 agTAACCGCTCTGGTGCAATTTATCTTTGGTTTAGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGa                                                                                                                                               <  2:467563/149‑1 (MQ=255)
    aaCCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAAc                                                                                                                                            <  1:730399/149‑1 (MQ=255)
    aaCCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAAc                                                                                                                                            <  1:1439862/149‑1 (MQ=255)
     aCCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACg                                                                                                                                           >  1:2017012/1‑149 (MQ=255)
     aCCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACg                                                                                                                                           >  2:747287/1‑149 (MQ=255)
      ccGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACgg                                                                                                                                          >  2:2416741/1‑149 (MQ=255)
       cGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGc                                                                                                                                         <  1:361146/149‑1 (MQ=255)
         ctctGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGt                                                                                                                                       >  2:1629333/1‑149 (MQ=255)
           ctGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCg                                                                                                                                     <  1:3301407/149‑1 (MQ=255)
            tGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCgg                                                                                                                                    <  2:662650/149‑1 (MQ=255)
             ggTGCATATTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGt                                                                                                                                   <  1:4058666/149‑1 (MQ=255)
             ggTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGt                                                                                                                                   <  1:3019757/149‑1 (MQ=255)
              gTGCAATTTATCTTTGGTTTCGTTCCACATTGGGGCTTTGGTCATTGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGtt                                                                                                                                  <  1:1645266/149‑1 (MQ=255)
              gTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGtt                                                                                                                                  <  1:2953246/149‑1 (MQ=255)
              gTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGtt                                                                                                                                  <  1:3721096/149‑1 (MQ=255)
              gTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGtt                                                                                                                                  <  1:3986880/149‑1 (MQ=255)
                gCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTc                                                                                                                                <  1:3073472/149‑1 (MQ=255)
                     ttATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGaa                                                                                                                           <  1:1121294/149‑1 (MQ=255)
                        tCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAacac                                                                                                                        >  2:1004478/1‑149 (MQ=255)
                        tCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAacac                                                                                                                        >  1:2793747/1‑149 (MQ=255)
                        tCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAacac                                                                                                                        <  2:4074942/149‑1 (MQ=255)
                         cTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACAcc                                                                                                                       >  1:2316131/1‑149 (MQ=255)
                          tttGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCa                                                                                                                      <  1:3894008/149‑1 (MQ=255)
                          tttGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCa                                                                                                                      <  1:1854243/149‑1 (MQ=255)
                           ttGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCAt                                                                                                                     >  1:60540/1‑149 (MQ=255)
                           ttGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCAt                                                                                                                     >  2:2272731/1‑149 (MQ=255)
                            tGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATg                                                                                                                    <  2:2882426/149‑1 (MQ=255)
                             ggTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGc                                                                                                                   <  2:2624664/149‑1 (MQ=255)
                              gTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGcc                                                                                                                  <  2:1151959/149‑1 (MQ=255)
                               tttCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGccc                                                                                                                 >  1:3934581/1‑149 (MQ=255)
                                   gTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACtt                                                                                                             >  1:3117559/1‑149 (MQ=255)
                                   gTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACtt                                                                                                             <  1:246474/149‑1 (MQ=255)
                                    ttCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACttt                                                                                                            >  1:2150712/1‑149 (MQ=255)
                                    ttCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACttt                                                                                                            <  2:667845/149‑1 (MQ=255)
                                    ttCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACttt                                                                                                            <  2:641658/149‑1 (MQ=255)
                                     tCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTc                                                                                                           >  1:2472204/1‑149 (MQ=255)
                                        acaATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgca                                                                                                        >  2:1310275/1‑149 (MQ=255)
                                          aaTGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgcacg                                                                                                      >  1:1347112/1‑149 (MQ=255)
                                             gCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACgcag                                                                                                   >  2:2987910/1‑149 (MQ=255)
                                                gCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGcagcag                                                                                                <  1:2682649/149‑1 (MQ=255)
                                                    tGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCg                                                                                            >  2:3009228/1‑149 (MQ=255)
                                                     ggTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGa                                                                                           >  2:1104201/1‑149 (MQ=255)
                                                     ggTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGa                                                                                           <  2:3632599/149‑1 (MQ=255)
                                                      gTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGat                                                                                          >  2:3909288/1‑149 (MQ=255)
                                                       tCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGata                                                                                         <  1:1809710/149‑1 (MQ=255)
                                                        cAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGTACACCATGCCCACTTTCGCACGCAGCAGTGCGatat                                                                                        <  2:1988188/149‑1 (MQ=255)
                                                          aTGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATcc                                                                                      >  2:2363121/1‑149 (MQ=255)
                                                          aTGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATcc                                                                                      <  1:3336501/149‑1 (MQ=255)
                                                             cccACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTga                                                                                   <  2:2796203/149‑1 (MQ=255)
                                                             cccACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTga                                                                                   <  1:4107886/149‑1 (MQ=255)
                                                               cACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTgaga                                                                                 <  2:1405937/149‑1 (MQ=255)
                                                               cACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTgaga                                                                                 >  2:45636/1‑149 (MQ=255)
                                                               cACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTgaga                                                                                 <  1:681314/149‑1 (MQ=255)
                                                                aCTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGagag                                                                                >  1:1331841/1‑149 (MQ=255)
                                                                 cTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGt                                                                               <  2:244478/149‑1 (MQ=255)
                                                                    cACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTgg                                                                            <  2:3379582/149‑1 (MQ=255)
                                                                           tCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAt                                                                     >  1:2314926/1‑149 (MQ=255)
                                                                             gTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgt                                                                   <  2:2570971/149‑1 (MQ=255)
                                                                              tCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgtt                                                                  >  1:1726253/1‑149 (MQ=255)
                                                                                cATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGGACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgttgt                                                                >  1:2883322/1‑149 (MQ=255)
                                                                                cATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgttgt                                                                <  2:1579970/149‑1 (MQ=255)
                                                                                cATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgttgt                                                                >  1:823594/1‑149 (MQ=255)
                                                                                 aTGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTc                                                               >  1:3377864/1‑149 (MQ=255)
                                                                                     cGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCa                                                           <  2:3081499/149‑1 (MQ=255)
                                                                                     cGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCa                                                           <  1:807427/149‑1 (MQ=255)
                                                                                       gCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATc                                                         <  1:2533378/149‑1 (MQ=255)
                                                                                       gCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATc                                                         <  2:920567/149‑1 (MQ=255)
                                                                                         aCGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCaa                                                       <  1:465221/149‑1 (MQ=255)
                                                                                          cGGGAGAGCTTCTCAAACAGACGATCGCCAATAGCGATGTTGTCGTAGATGGACATAGGAATCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATGCTGAGAGTTGGTCAGGATGTTGTCGCCTTCAAg                                                      <  2:559401/149‑1 (MQ=255)
                                                                                              agagCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGa                                                  >  2:1070169/1‑149 (MQ=255)
                                                                                              agagCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGa                                                  >  2:416586/1‑149 (MQ=255)
                                                                                               gagCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGaa                                                 >  2:3239769/1‑149 (MQ=255)
                                                                                                 gCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAtt                                               >  1:3499098/1‑149 (MQ=255)
                                                                                                 gCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAtt                                               <  2:1543953/149‑1 (MQ=255)
                                                                                                         aaCAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTc                                       >  1:1067891/1‑149 (MQ=255)
                                                                                                           cAGACGAACGCCAAAAGCGATGTTGTCTTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCATAATTTCACCTTCCg                                     >  1:1697051/1‑149 (MQ=255)
                                                                                                            aGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGc                                    >  1:1779807/1‑149 (MQ=255)
                                                                                                              aCGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAc                                  <  1:1859121/149‑1 (MQ=255)
                                                                                                                gAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgc                                <  2:3718592/149‑1 (MQ=255)
                                                                                                                gAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgc                                <  2:1673711/149‑1 (MQ=255)
                                                                                                                   cGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctgc                             <  2:2017012/149‑1 (MQ=255)
                                                                                                                    gCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgctgct                            >  2:4002927/1‑149 (MQ=255)
                                                                                                                     ccAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                           <  2:3550054/149‑1 (MQ=255)
                                                                                                                      cAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTcc                          <  1:445705/149‑1 (MQ=255)
                                                                                                                           gCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTa                     >  2:3112813/1‑149 (MQ=255)
                                                                                                                           gCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTa                     <  1:2272731/149‑1 (MQ=255)
                                                                                                                             gATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACa                   <  1:249646/149‑1 (MQ=255)
                                                                                                                              aTGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAg                  >  2:95892/1‑149 (MQ=255)
                                                                                                                              aTGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAg                  >  2:1766319/1‑149 (MQ=255)
                                                                                                                                    tCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaaa            <  1:3236244/149‑1 (MQ=255)
                                                                                                                                    tCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaaa            <  1:1469618/149‑1 (MQ=255)
                                                                                                                                       tAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCCGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAt         >  1:902/1‑149 (MQ=255)
                                                                                                                                       tAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAt         >  2:3508986/1‑149 (MQ=255)
                                                                                                                                        aGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAtt        >  1:1502080/1‑149 (MQ=255)
                                                                                                                                         gATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAttt       <  1:1738667/149‑1 (MQ=255)
                                                                                                                                            ggACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgt    <  1:3739311/149‑1 (MQ=255)
                                                                                                                                            ggACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgt    <  2:1702043/149‑1 (MQ=255)
                                                                                                                                              aCATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgttg  >  1:126583/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GAGTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTG  >  CP009273/3901269‑3901559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑